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Citations for Diagenode :
1 - 50 of 79 citations for Collagen Binding Fragment since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... we obtained 5 μg of high molecular weight genomic DNA (> 50% of fragments ≥ 40 kb) and sheared fragments to ~10 kb using the Megaruptor 3 (B06010003; Diagenode), followed by DNA repair and ligation of PacBio adapters using the SMRTbell Template Prep Kit v1.0 (100-991-900) ...
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bioRxiv - Genomics 2021Quote: ... Binding was stopped by adding 57 µL 2 M glycine (Diagenode, cat. C01011000) and incubating the sample for 5 min at room temperature ...
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bioRxiv - Evolutionary Biology 2022Quote: ... sheared DNA fragments with a Bioruptor Standard UCD-200 (Diagenode), and selected 150–750 bp fragments (excluding adapters) ...
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bioRxiv - Cancer Biology 2020Quote: ... and sonicated into 200-300bp fragments using a Biorupter (Diagenode). A 10% portion of the sheared chromatin was set aside as input DNA ...
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bioRxiv - Plant Biology 2021Quote: ... and sheared to generate 200-500bp fragments with Bioruptor (Diagenode) for 30 cycles (30s/on ...
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bioRxiv - Neuroscience 2022Quote: ... DNA fragments were then purified using IPure beads v2 (Diagenode, C01010055). Input (non-immunoprecipitated chromatin ...
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bioRxiv - Developmental Biology 2021Quote: ... Chromatin was sheared to 200–500-bp fragments using Bioruptor (Diagenode), and DNA fragments were precipitated with appropriate antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... and sonicated into 200–300 bp fragments using a Biorupter (Diagenode). A portion of the sheared chromatin (10% ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was sheared to 20 kb fragments using Megaruptor 2 (Diagenode) and size selected using the BluePippin system (Sage Science ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was sheared to 8 kb fragments using Megaruptor 2 (Diagenode). Standard SMRTbell libraries were prepared using Template Preparation Kit 1.0 (Pacific Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin was sheared to 200-500-bp fragments on a Bioruptor Plus (Diagenode; 2x 20-26 cycles of 30s “on” and 30s “off” at the highest power setting) ...
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bioRxiv - Genomics 2020Quote: ... chromatin was sheared to 200-500 bp fragments on a Bioruptor Plus (Diagenode; 2x 9 cycles of 30 sec on and 30 sec off at the highest power setting) ...
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bioRxiv - Cancer Biology 2020Quote: ... Chromatin was sheared into 200-400 bp fragments using a Bioruptor sonicator (Diagenode), clarified by centrifugation at max speed for 10 minutes ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was sheared to 20 kb fragments using the Megaruptor 2 (Diagenode) and size selected with a 10 kb cut-off using the BluePippin system (Sage Science) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 15-20 kb DNA fragments were obtained with Megaruptor (Diagenode, Denville, NJ, USA) and checked on a Fragment Analyzer (Advanced Analytical Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromatin was sheared to 200-500 bp fragments on a Bioruptor Plus (Diagenode; 5 × 5 cycles of 30 s on and 30 s off ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was sheared to 20-kb fragments using Megaruptor 2 (Diagenode, Belgium) and then bead-size selected with AMPure PB beads (Pacific Biosciences ...
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bioRxiv - Genomics 2023Quote: ... Chromatin was sheared to 200–500-bp fragments on a Bioruptor Plus (Diagenode; 2× 20–26 cycles of 30 s “on” and 30 s “off” at the highest power setting) ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin was sheared into ∼50–300 bp fragments by sonication using Bioruptor (Diagenode).
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bioRxiv - Genomics 2024Quote: ... proteinase inhibitor cocktail) and sonicated to fragments about 300 - 500 bp (Bioruptor, Diagenode). Dynabeads Protein A was washed twice with ChIP Buffer (10mM Tris-HCl pH7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Fixed cells were resuspended in 1 ml RIPA Binding Buffer and placed in the Bioruptor Pico (B01060010, Diagenode) for 10 cycles of 30 seconds on ...
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bioRxiv - Genetics 2019Quote: Purified DNA was fragmented into 500 bp fragments by sonication using Bioruptor Pico (Diagenode). Sequencing libraries were prepared using DNA Ultra II library preparation kit (NEB ...
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bioRxiv - Genomics 2019Quote: ... DNA from each sample was shared to 300 bp fragments using Bioruptor Pico (Diagenode), followed by end-repair and dA-tailing ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was sheared into ∼200 bp fragments by ultra-sonication (Bioruptor Plus Diagenode). Fragmented DNA samples were prepared for Illumina sequencing using the NEBNext Ultra I DNA Library Prep Kit for Illumina according to manufacturer’s instructions with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin was sheared to approximately 200bp-500bp fragments by sonication using a Bioruptor Plus (Diagenode) at high power ...
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bioRxiv - Genomics 2023Quote: ... The extracted DNA was sheared into 30 kb fragments using Megaruptor 2 (Diagenode, Seraing, Belgium), and then subjected to HiFi SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% SDS and sonicated to obtain approximately 200–500 bp chromatin fragments using a Bioruptor (Diagenode). Chromatin fragments were precleared with salmon sperm DNA (Trevigen ...
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bioRxiv - Developmental Biology 2019Quote: ... DNA was sheared into fragments ranging from 200 bp to 1000 bp using a sonicator (Diagenode Bioruptor Water Bath Sonicator ...
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bioRxiv - Immunology 2019Quote: ... followed by sonication to fragment chromatin to an average size of 200–500bp (Bioruptor NGS, Diagenode). Protein-DNA complexes were immuno-precipitated using 3μg of H3K27ac antibody from the same lot for all ChIP experiments (Catalogue #39133 ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were sonicated on ice to yield 200-600 bp DNA fragments using a Bioruptor (Diagenode).
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bioRxiv - Cancer Biology 2022Quote: ... and randomly fragmented (<500 bp fragments) using a Bioruptor® Pico sonication device (Diagenode, Denville, NJ). The fragments were ligated to adapters ...
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bioRxiv - Genomics 2023Quote: ... RNAse treated HMW gDNA was sheared to 20 kb fragments on the MegaRuptor™ device (Diagenode) and 10 μg sheared gDNA was used for library preparation ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10 ng sheared to an average fragment size of ∼300 bp (Diagenode Bioruptor Pico; B01060010) for enzymatic conversion / library preparation ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclear extracts were collected and sonicated for 10 minutes to obtain 300bp chromatin fragments (Diagenode Bioruptor Pico). Equal volumes of sheared chromatin were immunoprecipitated with rabbit AR-V7 antibody (RevMab 31-1109-00) ...
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bioRxiv - Genomics 2022Quote: ... 10 μg of DNA was purified then sheared to 20 kb fragments using the megaruptor1 system (Diagenode). For this library ...
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bioRxiv - Genomics 2022Quote: ... 90 μg of DNA was purified then sheared to 20 kb fragments using the megaruptor1 system (Diagenode). For each library ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA from this lysate was sheared to 300 bp fragments using a BioRuptor UCD-200 sonicator (Diagenode). Cell debris were removed by high-speed centrifugation and DNA from the supernatant was isolated by ethanol precipitation and resuspension in TE buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... These were sonicated to an average size of 200-500bp fragments on a Bioruptor Pico Sonicator (Diagenode) for 8 minutes 30 seconds ON/OFF at 4°C ...
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bioRxiv - Genomics 2020Quote: ... The chromatin was sheared to fragment size in the range of 200–500 bp using a Bioruptor (Diagenode). The solubilized chromatin fraction was cleared by centrifugation and used for immunoprecipitation after diluting 5 times with sonication buffer ...
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bioRxiv - Genomics 2019Quote: ... DNA was extracted using phenol:chloroform and sheared to 150bp fragments on a Bioruptor® Plus sonication device (Diagenode).
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bioRxiv - Genomics 2020Quote: ... Phage DNA was sheared to fragments of approximately 350 bp using a Diagenode Bioruptor® Pico (Diagenode Diagnostics). One μg of DNA for each phage isolate was used for Illumina Truseq library preparation using the manufacturer’s PCR-free library preparation protocol ...
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bioRxiv - Genomics 2022Quote: ... Sonication was carried out to shear chromatin into 200-500 bp size fragments using the Bioruptor Pico (Diagenode). 20 to 50 cycles of 30 seconds/cycle ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin was sheared to approximately 200 bp to 500 bp fragments by sonication using a Bioruptor Plus (Diagenode) at high power ...
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bioRxiv - Cell Biology 2022Quote: ... The amplified DNA (40 ng) was fragmented into ∼350 bp fragments using a Bioruptor® Sonication System (Diagenode Inc.). Illumina library construction was performed and qualified libraries were sequenced on the Illumina HiSeq platform ...
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bioRxiv - Cancer Biology 2020Quote: ... chromatin was sheared to 100-500bp fragments using the Bioruptor Pico sonicator for 3 cycles of 30 sec on/off in 0.5ml tubes (Diagenode). Quantitation of chromatin was performed using a qubit fluorimeter where at least 10 ng of chromatin were used per experiment ...
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bioRxiv - Plant Biology 2022Quote: ... the protocol involves fragmenting the HMW DNA to a mean fragment length of 20 kb using Megaruptor 3 (Diagenode), followed by DNA damage repair ...
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bioRxiv - Cancer Biology 2020Quote: ... which where lysed in a high-salt buffer (50mM Tris, 600mM NaCl, 10% glycerol, 0.2% NP40, 0.5mM DTT) followed by sonication to fragment chromatin (Diagenode Bioruptor). Buffers were supplemented with phosphatase and Complete ULTRA protease inhibitor cocktails (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... ATAC-seq library preparation involved PCR amplification of the purified DNA fragments using Diagenode primer indices (Diagenode, Cat# C01011034). Amplification cycles were adjusted and libraries were purified using AMPure beads ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The chromatin lysate was sonicated to a DNA fragment size range of 100-200bp using a Biorupter sonicator (Diagenode). CDK9 was immunoprecipitated with 15μg of an anti-CDK9 antibody (D-7 ...
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bioRxiv - Immunology 2020Quote: ... chromatin was sheared using a Bioruptor Pico Sonicator (5 cycles: 30 seconds ON - 30 seconds OFF, fragments 100 - 300 bp, Diagenode) and 10 % of sheared chromatin was kept as input control whilst 90 % was immunoprecipitated overnight using antibodies against H3K27ac ...