Labshake search
Citations for Diagenode :
251 - 300 of 346 citations for ADAM Metallopeptidase With Thrombospondin Type 1 Motif 9 ADAMTS9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... A portion of sheared DNA (10%) was kept as input and the rest of the sheared DNA was immunoprecipitated with α-5-methylcytosine antibody (Diagenode), bound to magnetic beads ...
-
bioRxiv - Genomics 2022Quote: ... and 800ul of antibody buffer containing 1ul of primary antibodies (normal rabbit IgG, Santa Cruz Cat no sc-2027, H3K27me3 (Millipore, Catalogue number 07-449, Lot DAM1703508 and H3K9me3 (Diagenode, Catalogue number C15410193 ...
-
bioRxiv - Cancer Biology 2020Quote: ... samples were incubated by rotating overnight at 4°C with primary antibody or irrelevant IgGs and then incubated with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 3 hr at 4 °C with rotation ...
-
bioRxiv - Plant Biology 2020Quote: ... the chromatin was sheared to an average length of 500 bp by sonication and then immunoprecipitated with the H3K9ac antibody (Diagenode, C15410004). The cross-linking was then reversed ...
-
bioRxiv - Microbiology 2021Quote: ... The presence of various histone methylation marks with PfCD bound nucleosome complexes were analyzed using modification specific antibodies against H3K9me3 (Diagenode # C15410193), H3K36me3 (Diagenode # C15410192) ...
-
bioRxiv - Developmental Biology 2021Quote: ... 10% goat serum in PBST was used to block embryos before addition of pre-blocked Pol II S2P monoclonal antibody (Diagenode, C15200005) at 1:1000 dilution ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
-
bioRxiv - Genomics 2023Quote: ... A portion of sheared DNA (10 %) was kept as input and the rest of the sheared DNA was immunoprecipitated with α-5-methylcytosine antibody (Diagenode), bound to magnetic beads ...
-
bioRxiv - Genomics 2023Quote: ... Approximately 10 μg chromatin was aliquoted and diluted to 500 μL of 1x ChIP Buffer and immunoprecipitated using 1.4 μg anti-H3K4me3 antibody (pAb-003-050, Diagenode, Denville, NJ). In some cases ...
-
bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref: 901503; anti-H3K4me3 Cell Signaling, ref. 9751S; anti-H3K36me3 Diagenode, ref ...
-
Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... and mouse anti-ERalpha (1:200; # C15100066, Diagenode, Liège, Belgium); negative controls were proceeded without one primary antibody or both ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1% SDS and chromatin was fragmented using a Bioruptor (Diagenode) sonicator to obtain an average size of 250–300 base pairs ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1 mM PMSF) and lysed using Bioruptor Plus sonicator (Diagenode). Protein purification was carried out at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... 600 ng of denatured DNA was incubated with 2 mg of 5-methylcytosine (5-mC) monoclonal antibody ([33D3] Diagenode, Cat No # C15200081) in IP buffer (0.1% SDS ...
-
bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
-
bioRxiv - Bioengineering 2020Quote: ... rabbit polyclonal anti-Cas9 (C15310258-100, 1:1000, Diagenode, Denville, NJ) and rabbit monoclonal anti-Gapdh (#2118 ...
-
bioRxiv - Microbiology 2021Quote: ... anti-Histone 4 rabbit (1:2,000; Diagenode Cat. No C15410156-50). HRP-conjugated anti-mouse and anti-rabbit antibodies were used (1:5,000 ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1% SDS) and then sonicated on ice using a Bioruptor (Diagenode) to obtain sheared chromatin ranging between 100–500 bp ...
-
bioRxiv - Molecular Biology 2022Quote: ... incubated in blocking buffer with 1:500 rat anti-5hmC (Diagenode) overnight at 4°C and followed by incubation with 1:1000 anti-rat IgG HRP (ab6734 ...
-
bioRxiv - Genomics 2023Quote: ... or pA-Tn5 Transposase - loaded (Diagenode C01070001; Lot 1/b/b).
-
bioRxiv - Neuroscience 2021Quote: ... equal volume of sheared chromatin was incubated overnight with 3 μg of antibody in iC1b buffer with protease inhibitors and BSA and washed DiaMag Protein A-coated magnetic beads (Diagenode Cat# C03010020-220) on a tube rotator at 4°C overnight ...
-
bioRxiv - Genetics 2021Quote: ChlPseq was performed on 20k FACS sorted MM cells as previously described 44 using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) but with minor modifications on the preparation of input controls ...
-
bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1.2 μg total protein was analysed with Cas9 (Diagenode C15310258, 1:400) and GAPDH (EMD MAB374 ...
-
bioRxiv - Cell Biology 2021Quote: ... 1% Sodium Deoxycholate and 0.5 mM EGTA) using the Bioruptor Sonicator (Diagenode) with 14 cycles of 15s on/15s off using the highest amplitude ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1% Triton X-100) and subjected to sonication using Bioruptor Standard (Diagenode). After centrifugation ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1% SDS) and sonicated using the Bioruptor plus water bath system (Diagenode, 16 cycles at high power ...
-
bioRxiv - Systems Biology 2022Quote: 1 μg of purified DNA was sonicated using the Bioruptor Pico (Diagenode) for 15 cycles of 30 second ON / 90 seconds OFF ...
-
bioRxiv - Plant Biology 2024Quote: ... The chromatin was incubated with 1 μg of α-H3K27me3 (C15410195 Diagenode), α-H3K4me3 (C15410003 ...
-
bioRxiv - Cell Biology 2020Quote: ... Chromatin was sonicated to approximately 1 nucleosome using a Bioruptor waterbath sonicator (Diagenode). Chromatin of ∼5×106 cells was incubated with 3ug antibody (RNAPII ...
-
The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... Overnight IP was performed using 1 μg of anti-H3K4me3 (Diagenode, C1541003-50), 2 μg anti-H3K27me3 (Diagenode ...
-
bioRxiv - Genomics 2020Quote: ... and probed with rabbit pAb CRISPR/Cas9 (C15310258, Diagenode, Liège, Belgium, 1:5000), rabbit pAb GFP (ab290 ...
-
bioRxiv - Microbiology 2021Quote: ... 1 μg of gDNA was fragmented by mechanical shearing using a bioruptor (Diagenode) using the shearing profile 30 s ON ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... and sheared between 15kb to 20 kb using the Megaruptor 1 system (Diagenode). For Morpho amathonte ...
-
bioRxiv - Genomics 2023Quote: ... The resulting chromatin was incubated overnight with the following antibodies (H3K27ac, Diagenode, Catalog No: C15410196 LOT: A1723-0041D; H3K4me2, Diagenode, Catalog No: C15410035 LOT: A936-0023) coupled with 40 μl protein A and protein G beads (Invitrogen ...
-
The epigenomic landscape regulating organogenesis in human embryos linked to developmental disordersbioRxiv - Genomics 2019Quote: ... 1% SDS and protease inhibitor cocktail) and sonicated under prior optimised conditions (Diagenode Bioruptor). Sufficient sample was prepared to allow in parallel immunoprecipitation for H3K4me3 ...
-
bioRxiv - Neuroscience 2019Quote: ... Nuclei diluted in 1 ml RIPA buffer were sonicated using Bioruptor (Diagenode, Denville, NJ) for 6 cycles of 5 minutes (60 seconds on/30 seconds off ...
-
bioRxiv - Microbiology 2023Quote: ... bacteria were bath-sonicated for 1 min at medium amplitude on a Bioruptor (Diagenode) to break bacterial chains and plated on plates with suitable antibiotics to select for transformants.
-
bioRxiv - Molecular Biology 2021Quote: ... Chromatins were sheared 4x 20 sec ON/1 min OFF with Bioruptor Pico sonicator (Diagenode), then diluted four times with dilution buffer (10 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT) and sonicated for 3x 10 seconds using a Bioruptor Pico (Diagenode). Cells were then rotated at 4°C for 20 mins before the lysates were diluted with 500 µl of no salt buffer (50 mM Tris-HCl ...
-
bioRxiv - Plant Biology 2019Quote: ... Loose chromatin was removed with 1% SDS prior to sonication on a Bioruptor™ (Diagenode). The immunoprecipitation step involved incubation with different antibodies (Flag M2 beads (sigma ...
-
bioRxiv - Genomics 2023Quote: ... Crosslinked chromatin resuspended in 1 ml LB3 was then sonicated using a BioRuptor Pico (Diagenode) for 30 cycles of 30 seconds on/off ...
-
bioRxiv - Developmental Biology 2021Quote: ... Immunoprecipitation was done using 15-20 μg of DNA and 1 μg of CTCF Ab (Diagenode:C15410210) and all steps were performed following the manufacturer instructions ...
-
Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... The eluates were diluted 1:50 with ChIP buffer from the One Day ChIP kit (Diagenode) and subsequently subjected to a second ChIP in accordance with the One Day ChIP kit manual but performing an overnight antibody incubation under rotation at 4°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1% SDS and sonicated to obtain approximately 200–500 bp chromatin fragments using a Bioruptor (Diagenode). Chromatin fragments were precleared with salmon sperm DNA (Trevigen ...
-
bioRxiv - Cell Biology 2019Quote: ... 1 mM MgCl2,) and sonicated 10x with an interval of 30’’ on “medium” setting (Bioruptor300; Diagenode). Protein concentrations were measured using Quick Start™ Bradford 1x Dye Reagent from BIO RAD according to the manufacturers manual and equal amounts loaded for each lane.
-
bioRxiv - Developmental Biology 2022Quote: ... 1% SDS was then added followed by 3x 30sec sonication steps with a bioruptor sonicator (Diagenode) to solubilize the chromatin ...
-
bioRxiv - Microbiology 2023Quote: ... 1 ug of genomic DNA was sheared by sonication using a Picorupter (Diagenode s.a., Liege, Belgium) to obtain fragments with an average size of 300 bp ...
-
bioRxiv - Molecular Biology 2020Quote: ... Libraries were generated from 1 ng of DNA using the MicroPlex Library Preparation Kit v2 (C05010012, Diagenode) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... mRNA was adjusted to about 10Lng μl−1 in 100Lμl and fragmented using a Bioruptor ultrasonicator (Diagenode) with 30Ls on/off for 30 cycles ...