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Citations for Diagenode :
1 - 50 of 104 citations for 7 nitro 9 oxo 9H fluorene 2 sulfonylchloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... α-Synuclein PFFs were vortexed and diluted with DPBS to 2 mg/mL and sonicated in a cooled bath sonicator at 9°C (Diagenode Bioruptor® Pico ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies (AR, 9 μg, Abcam, ab74272; H3K27ac, 1 μg, Diagenode, C15410196) were incubated with 40 μL of protein A/G Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Tau was sonicated in a cooled bath sonicator at 9°C (Diagenode Bioruptor® ...
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bioRxiv - Microbiology 2021Quote: ... The DNA of each sample was sheared at 9 kb using the Megaruptor system (Diagenode) and then barcoded using the SMRTbell Barcoded Adapter Prep Kit (Pacific Bioscience ...
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bioRxiv - Molecular Biology 2023Quote: ... and chromatin was sheared by sonication using a Bioruptor (Diagenode, 9 cycles of 30 s on/off). Extracts were incubated for 2 h at room temperature with anti-V5 agarose beads (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... while Lab 2 used Antibody 2 (Diagenode, Denville ...
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bioRxiv - Genomics 2020Quote: ... nuclei were isolated via sonication using a Bioruptor Pico (Diagenode; 9 cycles of 10 sec on and 30 sec off). Chromatin was then sheared in the recommended shearing buffer (27-30 cycles ...
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bioRxiv - Immunology 2022Quote: ... with a freshly added protease inhibitor cocktail and sodium vanadate and subjected to 9 cycles (30s ON-10s OFF) of ultrasonication (Bioruptor Plus; Diagenode). The sonicated samples are then spun down ...
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bioRxiv - Neuroscience 2022Quote: ... using 7 cycles for chromatin shearing on a Bioruptor Pico sonicator (Diagenode, Cat. No. B01060001). Prior to sonication ...
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bioRxiv - Molecular Biology 2019Quote: ... added (Figure 5B-5D) or 2x 300 µl was sonicated in 2x 1.5 ml bioruptor pico microtubes (Diagenode, Figure 6-9). When using the APLP protease inhibitors ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13.000 rpm and 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13,000 rpm and 4 °C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... approximately 7 µg of DNA was sheared at 20 kb using the megaruptor system (Diagenode, Liege, Belgium). A DNA damage repair step was performed on 5 µg of DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... The DNA was sheared by sonication (4x 7 cycles, 30s on / 30s off, power high; Bioruptor, Diagenode) at 4°C and lysates cleared by centrifugation at 16,100 x g at 4°C for 15 min ...
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bioRxiv - Genomics 2023Quote: ... a Megaruptor 2 (Diagenode) was used to shear 3 μg of DNA to 15kb fragments ...
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bioRxiv - Immunology 2022Quote: ... Sonicated chromatin was diluted ten times in ChIP Dilution Buffer and immunoprecipitation was done overnight at 4°C by incubating 2.5 μl (7 μg) of anti-H3K27ac antibody (C15410196; Diagenode) precoated on 25-μl protein A-coated magnetic beads (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... After this time, samples were sonicated twice (7 cycles 30s on, 30s off) with a Bioruptor Plus sonicator (Diagenode). In between the two sonication cycles samples were incubated at 12°C with 0.8ul of Benzonase (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin samples were sonicated for 7 cycles (30 s ON, 30 sec OFF, at High output) in a Bioruptor (Diagenode). The samples were heated to 95°C for 5 min and stored at -20°C ...
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bioRxiv - Cell Biology 2023Quote: ... H3K27me3 (Diagenode; #C15410195; 2 μg) and IgG-rabbit (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... H3K9me3 (Diagenode; #C15410193; 2 μg), H3K27me3 (Diagenode ...
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bioRxiv - Genetics 2021Quote: ... 3 to 7 days later individual colonies were picked and expanded and western blot was used to confirm Cas9 protein expression (Diagenode, C15200203). Cas9 expression in a high expressing line was confirmed by immunohistochemistry (5).
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bioRxiv - Systems Biology 2023Quote: ... 100 mM Tris-HCl pH 8.5) and boiled for 7 min at 95 °C before high-energy sonication to shear DNA (Diagenode Bioruptor pico). Protein concentrations were determined via a BCA assay (Thermo Fisher) ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... 2 μg anti-H3K27me3 (Diagenode, C15410195), and 2.5 μg anti-enhancer of zeste homolog 2 (EZH2 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 µg of 5mC antibody (Diagenode) were added to the sample and incubated for 2 h at 4°C with rotation ...
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bioRxiv - Microbiology 2019Quote: ... 30 seconds off) for 7 cycles of sonication in a 4°C water bath using the Bioruptor Sonication System (Diagenode, Denville, NJ) at the high-intensity setting ...
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bioRxiv - Cell Biology 2020Quote: ... anti-H4K20me3 (2 μl/ChIP, C15410207, Diagenode), anti-PA28γ (0.5 μl /ChIP ...
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bioRxiv - Cell Biology 2020Quote: ... anti-H3K9me3 (2 μl/ChIP, C15410056 Diagenode), anti-H3K4me3 (1 μl/ChIP ...
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bioRxiv - Microbiology 2021Quote: ... gDNA was sheared using Megaruptor 2 (Diagenode) to an average size of 8.9kb ...
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bioRxiv - Genomics 2021Quote: ... or 2 µg IgG control (Diagenode C15410296) was used ...
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bioRxiv - Genomics 2021Quote: ... and sheared DNA was added to antibodies coupled to washed magnetic beads (anti-FLI1 2 µg, ab15289, Abcam; anti-ERG 2 µg, ab92513, Abcam; anti-H3K4me3 1.4 µg, C15410003, Diagenode; H3K27me3 2.9 µg ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 2% (v/v) 2-mercaptoethanol and samples were sonicated using a Bioruptor sonicator (Diagenode SA, Seraing, Belgium) at high amplitude for five 30 s on and off cycles ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was resuspended in ice-cold lysis buffer (1xPBS, 2 mM PMSF, 2 mM DTT, 1 x protease inhibitors (Pierce)) and sonicated (Bioruptor Pico, Diagenode) at 4LJ with alternating 10 s burst/10 s break ...
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bioRxiv - Microbiology 2021Quote: ... but rather using the Megaruptor 2 system (Diagenode) with the software settings at 20Kb and following their user manual ...
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bioRxiv - Genetics 2022Quote: ... 0.5 μg (2 ul) of H3K9me3 antibody (Diagenode) and 0.5 μL of biotin-myc antibody (Abcam ...
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bioRxiv - Genomics 2023Quote: ... Size selection was performed with Megaruptor 2 (Diagenode) with default settings ...
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bioRxiv - Genomics 2020Quote: ... genomic DNA was sheared using the Megaruptor 2 (Diagenode) with a long hydropore and a 20 kb shearing protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg DNA was sonicated in a Bioruptor Plus (Diagenode). DamID adaptors were removed by AlwI digestion ...
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bioRxiv - Developmental Biology 2019Quote: ... subtracted to 2 cycles of sonication on a bioruptor (Diagenode) with 30 sec “ON”/“OFF” at low setting and the remaining cell debris was removed by centrifugation at 21,000 g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... boiled for 5 min at 95°C 1000rpm in 2% SDC buffer (2% SDC, 100 mM Tris-HCl pH=8.5) and sonicated (Diagenode Bioruptor, 15 * 30s at high intensity). Protein concentration was determined via BCA Protein Assay (Thermo ...
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bioRxiv - Genomics 2023Quote: ... The samples were fragmented to 10 kb using Megaruptor 2 (Diagenode). For each fragmented sample ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of antibodies ChIP-grade (H3K4me2, Abcam ab32356; H3K4me3, Diagenode C15410003 ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was sheared to 20 kb fragments using Megaruptor 2 (Diagenode) and size selected using the BluePippin system (Sage Science ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was sheared to 8 kb fragments using Megaruptor 2 (Diagenode). Standard SMRTbell libraries were prepared using Template Preparation Kit 1.0 (Pacific Biosciences ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... and 2.5 μg anti-enhancer of zeste homolog 2 (EZH2) (Diagenode, C15200180). Antibody-protein complexes were immunoprecipitated with protein A/G Dynabeads for 3 hours at 4°C with rotation ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were synthesized using a MicroPlex Library Preparation Kit (v.2; Diagenode). Libraries were sequenced as 2 × 50-bp reads on an Illumina HiSeq 2500 instrument ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 ug/ul of lysates were sonicated with a Bioruptor Plus (Diagenode) for 20 cycles (30 seconds ON and 30 seconds OFF) ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was fragmented to 20 kb using Megaruptor 2 (Diagenode, Liege, Belgium) and size selected with a 10 kb cut-off using the Blue Pippin system (Sage Science ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was sheared to 20 kb fragments using the Megaruptor 2 (Diagenode) and size selected with a 10 kb cut-off using the BluePippin system (Sage Science) ...
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bioRxiv - Genomics 2021Quote: ... Binding was stopped by adding 57 µL 2 M glycine (Diagenode, cat. C01011000) and incubating the sample for 5 min at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was sheared to 20-kb fragments using Megaruptor 2 (Diagenode, Belgium) and then bead-size selected with AMPure PB beads (Pacific Biosciences ...