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Citations for Diagenode :
351 - 364 of 364 citations for 7 Oxabicyclo 4.1.0 heptane 2 carboxylicacid 6 ethyl 1 methyl 5 oxo ethylester 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA (1 μg) was fragmented to 300–600 bp after 25 cycles of 30 s of pulsed sonication in Bioruptor (Diagenode, Belgium). Fragmented DNA was end-repaired and adapter ligation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were kept on ice and processed directly or frozen at −80C in 20 ml of RNAse/DNAse free PBS supplemented with 1× protein inhibitor (Diagenode, Belgium). Embryos younger than late gastrula (younger than E7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Each chromatin sample containing about 6,000 cells was incubated with 1 µg of anti-H3K27me3 antibody (Diagenode, C15410195, lot. A1811-001P). ChIP was performed using the automated platform SX-8G IP-Star platform (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... in 10 µl nuclease-free H2O and amplified with NEBNext® High-Fidelity 2X PCR Master Mix and 1 ul of primer pair from Diagenode (24 SI for tagmented libraries ...
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bioRxiv - Plant Biology 2023Quote: ... and cOmplete protease inhibitor cocktail tablets) and proceeded for sonication to shear the chromatin (∼ 500 bp to 1 kb size fragments) using Bioruptor Plus (Diagenode, USA) set at low mode and 45 cycles of 30 sec ON ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 mM PMSF) and chromatin was sheared by sonication to approximately 500-100 bp fragments using the Bioruptor® sonicator (Diagenode, Belgium). Immunoprecipitation was done with the anti-H3K27me3 (Active-motif ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Plant Biology 2023Quote: ... Slides were blocked with 4% BSA for 30 minutes at 37°C in a moist box followed by primary (anti-H3K9m2, C15410060, Diagenode, 1:500) and secondary (Alexa Fluor 488 Goat anti-Rabbit IgG1 Secondary Antibody ...
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bioRxiv - Genomics 2022Quote: ... libraries were constructed with 200 ng input DNA from each sample (n = 24; Table 1) using the Premium RRBS Kit (Diagenode, Cat. No. C02030032) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... PFFs were diluted in 1× PBS to a final concentration of 100 μL/Ml and sonicated with Bioruptor sonication device (Diagenode, Liege, Belgium, #B01020001) at the following settings ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were crosslinked with 1% PFA at room temperature for 10 minutes and sonicated for 11 cycles (30 s ON/OFF, Diagenode Bioruptor Pico bath sonicator). 5% of the lysate was reserved as Input ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS and 0.5% N-lauroylsarcosine) and the chromatin was fragmented by sonication for 10 cycles on Bioruptor (Diagenode high settings, 30’’ on/off). Chromatin was separated from cell debris using centrifugation and stored at -80C for maximum of 2 months.
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bioRxiv - Neuroscience 2024Quote: ... The nuclear pellets were lysed with lysis buffer (50 mM Tris-HCl, 10 mM EDTA, 1% SDS, pH 8) and then sonicated with Bioruptor (Diagenode, 15 cycles, 30s ON/30s OFF). The size of the DNA in the sheared chromatin fragments was checked before precipitation by a Tapestation system (Agilent 4200 ...