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Citations for Diagenode :
301 - 350 of 369 citations for 7 Fluoro 6 amino 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Chromatin was sonicated to approximately 1 nucleosome using a Bioruptor waterbath sonicator (Diagenode). Chromatin of ∼5×106 cells was incubated with 3ug antibody (RNAPII ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... Overnight IP was performed using 1 μg of anti-H3K4me3 (Diagenode, C1541003-50), 2 μg anti-H3K27me3 (Diagenode ...
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bioRxiv - Genomics 2020Quote: ... and probed with rabbit pAb CRISPR/Cas9 (C15310258, Diagenode, Liège, Belgium, 1:5000), rabbit pAb GFP (ab290 ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were applied: H3K4me1 (1:100, C15410037, Diagenode), H3K27ac (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of gDNA was fragmented by mechanical shearing using a bioruptor (Diagenode) using the shearing profile 30 s ON ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and sheared between 15kb to 20 kb using the Megaruptor 1 system (Diagenode). For Morpho amathonte ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by immunostaining with Cas9 monoclonal antibody 4G10 (Diagenode cat#: C15200216; 1:200 dilution) with gentle shaking for 2h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... The following antibodies were used for western blot analysis: Cas9 (Diagenode C15310258, 1:1000) TCF4 (Abcam ab217668 ...
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The epigenomic landscape regulating organogenesis in human embryos linked to developmental disordersbioRxiv - Genomics 2019Quote: ... 1% SDS and protease inhibitor cocktail) and sonicated under prior optimised conditions (Diagenode Bioruptor). Sufficient sample was prepared to allow in parallel immunoprecipitation for H3K4me3 ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei diluted in 1 ml RIPA buffer were sonicated using Bioruptor (Diagenode, Denville, NJ) for 6 cycles of 5 minutes (60 seconds on/30 seconds off ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were bath-sonicated for 1 min at medium amplitude on a Bioruptor (Diagenode) to break bacterial chains and plated on plates with suitable antibiotics to select for transformants.
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bioRxiv - Molecular Biology 2021Quote: ... Chromatins were sheared 4x 20 sec ON/1 min OFF with Bioruptor Pico sonicator (Diagenode), then diluted four times with dilution buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT) and sonicated for 3x 10 seconds using a Bioruptor Pico (Diagenode). Cells were then rotated at 4°C for 20 mins before the lysates were diluted with 500 µl of no salt buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... Loose chromatin was removed with 1% SDS prior to sonication on a Bioruptor™ (Diagenode). The immunoprecipitation step involved incubation with different antibodies (Flag M2 beads (sigma ...
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bioRxiv - Systems Biology 2023Quote: ... ∼400 ng DNA-containing chromatin was incubated with 1 µg antibody (H3K9/14ac (Diagenode, C15410005); H3K9me2 (in house ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunolabeling of Cas9 protein by monoclonal anti-mouse Cas9 antibody at 1:500 (4G10; Diagenode) was blocked in PBS Super Block (37580 ...
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bioRxiv - Genomics 2023Quote: ... Crosslinked chromatin resuspended in 1 ml LB3 was then sonicated using a BioRuptor Pico (Diagenode) for 30 cycles of 30 seconds on/off ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunoprecipitation was done using 15-20 μg of DNA and 1 μg of CTCF Ab (Diagenode:C15410210) and all steps were performed following the manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% SDS and sonicated to obtain approximately 200–500 bp chromatin fragments using a Bioruptor (Diagenode). Chromatin fragments were precleared with salmon sperm DNA (Trevigen ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM MgCl2,) and sonicated 10x with an interval of 30’’ on “medium” setting (Bioruptor300; Diagenode). Protein concentrations were measured using Quick Start™ Bradford 1x Dye Reagent from BIO RAD according to the manufacturers manual and equal amounts loaded for each lane.
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bioRxiv - Cancer Biology 2020Quote: ... using 1 µg antibody (anti-H3K4me1, #ab8895, Abcam; anti-H3K27ac, #ab4729, Abcam; anti-H3K4me3, #c15410003, Diagenode). 10% of the sheared chromatins were kept aside as input samples ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% SDS was then added followed by 3x 30sec sonication steps with a bioruptor sonicator (Diagenode) to solubilize the chromatin ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin of each replicate was incubated with 1 μg of antibody against H3K27ac (Diagenode, C15410196, RRID:AB_2637079) or 1 μg of antibody against ONECUT2 (Proteintech ...
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bioRxiv - Microbiology 2023Quote: ... 1 ug of genomic DNA was sheared by sonication using a Picorupter (Diagenode s.a., Liege, Belgium) to obtain fragments with an average size of 300 bp ...
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bioRxiv - Immunology 2024Quote: ... Beads were blocked with 1 µL of antibody (α-H3K27ac, Diagenode, C15410196; α-H3K4me3, Diagenode, C15410003) in antibody binding buffer for 3-4h at 4°C with rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were generated from 1 ng of DNA using the MicroPlex Library Preparation Kit v2 (C05010012, Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was adjusted to about 10Lng μl−1 in 100Lμl and fragmented using a Bioruptor ultrasonicator (Diagenode) with 30Ls on/off for 30 cycles ...
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bioRxiv - Molecular Biology 2021Quote: ... Fixed cells were resuspended in 1 ml RIPA Binding Buffer and placed in the Bioruptor Pico (B01060010, Diagenode) for 10 cycles of 30 seconds on ...
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was resuspended in 400µL of lysis buffer (1% SDS, 50mM Tris-HCl pH 7.5) and sonicated with the Pico Bioruptor (Diagenode) for 20 cycles (30sec ON/ 30sec OFF ...
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bioRxiv - Genomics 2023Quote: ... and 1 µg of DNA was purified and then sheared at 13 kb using the Megaruptor1 system (Diagenode). The library was size-selected ...
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bioRxiv - Biochemistry 2024Quote: ... 1 x Halt Protease Inhibitor Cocktail) and water-bath sonicated (Diagenode; 25 cycles, 30 sec on and off). Sheard chromatin was diluted to achieve 0.1 % SDS and incubated with FOXA1 antibody (Novus Biologicals ...
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bioRxiv - Genomics 2019Quote: ... at 0.3J/cm2, and then lysed (1% SDS, 50mM Tris pH7.5, 500 mM NaCl) and sonicated using a Bioruptor (Diagenode) (six cycles 30” on 45” off) ...
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bioRxiv - Cell Biology 2021Quote: ... Pelleted cells were resuspended in 1 ml 50 mM Tris pH 7.5 and sonicated in a Bioruptor Twin (Diagenode) on LOW 30 sec ON ...
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bioRxiv - Immunology 2020Quote: ... The cells were cross-linked with 1% formaldehyde for 10 minutes at RT and quenched with 2.5 M glycine (Diagenode) for 5 minutes at RT ...
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bioRxiv - Neuroscience 2023Quote: ... 1:100) followed by sonication for 16 cycles (bursts and intervals of 30s each) with the Bioruptor® (Diagenode), which produced 200-1000 bp genomic DNA fragments ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease and phosphatase inhibitor cocktails and sonicated for 1 min with the Bioruptor sonication device (Diagenode). Cell lysates were centrifuged at 13,000 × g for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... the pellets were resuspended in 300 μL ChIP lysis buffer containing 1 mM PMSF and sonicated for 15 min (30s ON - 60s OFF) in a Bioruptor (Diagenode). The lysates were centrifuged at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed in 1 ml extraction buffer by three freeze-thaw cycles using liquid nitrogen and by sonication using a Bioruptor (Diagenode) with one 30 s high power pulse ...
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bioRxiv - Cell Biology 2021Quote: ... and for 15 minutes at 100°C in a proteinase K/elution buffer mix (1 µl of proteinase K in 100 µl DIB-buffer; Diagenode). Capture efficiency was determined by qPCR against spiked-in Lambda-DNA fragments in precapture and postcapture library samples ...
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bioRxiv - Genomics 2022Quote: ... and chromatin was fragmented into 1–5-kb fragments by sonication (high level, 33 cycles, 30 s ON, 50 s OFF) using a Bioruptor (Diagenode). Chromatin immunoprecipitation was used to enrich the complex fragments with magnetic beads of protein G (Thermo ...
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bioRxiv - Neuroscience 2022Quote: ... cells were cross-linked with 1% paraformaldehyde for 15 minutes and Chromatins were sheared into an average 200–400 bp in length by sonication (Diagenode) and immunoprecipitated with following antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... rested for 1 min on ice and sonicated (20 cycles: 15 seconds on/off; Bioruptor plus model UCD-300, Diagenode). Reduction and alkylation of disulfide bridges was performed by incubating the samples at 95 °C for 5 minutes ...
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bioRxiv - Physiology 2020Quote: ... Resulting nuclei were centrifuged at 12,000 RPM and resuspended in shearing buffer (1% SDS, 10mM EDTA pH 8, 50mM Tris pH8) and sheared in 1.5ml Bioruptor Microtubes (Diagenode, C30010016) in a Bioruptor (Diagenode ...
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bioRxiv - Genetics 2020Quote: S2 cells transfected with either mCherry-Nnk or Odj-mCherry for 72 hours were fixed with 1% paraformaldehyde for 10 minutes and sheared with Bioruptor sonicator (Diagenode) to obtain chromatin ...
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bioRxiv - Molecular Biology 2019Quote: ... The digestion was then stopped with 5mM EDTA and the digested chromatin was solubilized in 1% SDS and three pulses of 30sec sonication using a Bioruptor ultrasonic cell disruptor (Diagenode). 200µg of pre-cleared chromatin was immunopurified with incubation of 30µl of Anti-FLAG M2 Affinity Gel (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... SDS was then added to a final concentration of 1% followed by a 3x 30sec sonication steps with a bioruptor sonicator (Diagenode). Insoluble precipitates were removed by centrifugation and soluble crosslinked chromatin extracts were diluted 10x in IP buffer (0.3M Sucrose ...
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bioRxiv - Neuroscience 2020Quote: ... PFFs are diluted in PBS and sonicated for 10 cycles (1 sec ON, 30 sec OFF, high intensity) in a bath sonicator at 10°C (BioRuptor; Diagenode).
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bioRxiv - Molecular Biology 2021Quote: ... cells were cross-linked for 10 min at room temperature with 1% formaldehyde before lysis followed by sonication of their chromatin (Bioruptor Plus, Diagenode) to obtain DNA fragments of 200–400 bp ...
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bioRxiv - Plant Biology 2023Quote: ... Nuclei were lysed in NLB (50 mM Tris-HCl pH8, 10 mM EDTA, 1% SDS and 1x Proteases Inhibitor cocktail. Chromatin was sonicated using a Diagenode Bioruptor (40 Hz ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed on ice for 10 minutes prior to sonication for either 20 cycles (replicate 1) or 40 cycles (replicate 2) of 30s on / 30s off with a Bioruptor UCD-200 (Diagenode) at maximum power ...