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Citations for Diagenode :
1 - 50 of 88 citations for 7 Bromo 1H indazole 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... treated by RNase A (20 µg/mL) for 1h at 37°C in TE buffer and sonicated 8 min with BioRuptor (Diagenode) (15 sec ON / 15 sec OFF ...
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bioRxiv - Neuroscience 2022Quote: ... using 7 cycles for chromatin shearing on a Bioruptor Pico sonicator (Diagenode, Cat. No. B01060001). Prior to sonication ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13.000 rpm and 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13,000 rpm and 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Nucleic acids were fragmented with a Diagenode Bioruptor® Sonicator (Diagenode, Belgium). For immunoprecipitation ...
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bioRxiv - Evolutionary Biology 2019Quote: ... approximately 7 µg of DNA was sheared at 20 kb using the megaruptor system (Diagenode, Liege, Belgium). A DNA damage repair step was performed on 5 µg of DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... The DNA was sheared by sonication (4x 7 cycles, 30s on / 30s off, power high; Bioruptor, Diagenode) at 4°C and lysates cleared by centrifugation at 16,100 x g at 4°C for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of antibody per IP (Cas9 – Diagenode C15310258 ...
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bioRxiv - Genomics 2021Quote: ... Labs 1 and 3 used Antibody 1 (Diagenode, Denville ...
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bioRxiv - Genomics 2024Quote: ... DNA was then sheared with Megaruptor 3 (Diagenode) (45/200/30 ...
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bioRxiv - Immunology 2022Quote: ... Sonicated chromatin was diluted ten times in ChIP Dilution Buffer and immunoprecipitation was done overnight at 4°C by incubating 2.5 μl (7 μg) of anti-H3K27ac antibody (C15410196; Diagenode) precoated on 25-μl protein A-coated magnetic beads (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... After this time, samples were sonicated twice (7 cycles 30s on, 30s off) with a Bioruptor Plus sonicator (Diagenode). In between the two sonication cycles samples were incubated at 12°C with 0.8ul of Benzonase (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 µg of either IgG control antibody (Diagenode; C15410206) or of a specific antibody were applied ...
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bioRxiv - Cell Biology 2023Quote: ... sonicated 3 × 30 s (power: middle, Bioruptor Plus, Diagenode) and then centrifuged at 16000 g for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin samples were sonicated for 7 cycles (30 s ON, 30 sec OFF, at High output) in a Bioruptor (Diagenode). The samples were heated to 95°C for 5 min and stored at -20°C ...
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bioRxiv - Immunology 2019Quote: ... 3 µL protease inhibitor cocktail 1µg of H3K27ac antibody (Diagenode) and incubated overnight at 4 ºC with rotation ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was sheared with the Megaruptor 3 system (Diagenode) using 35-40 cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... sheared to 3-5kb in length with Megaruptor (Diagenode Diagnostics), and used to generate sequencing libraries for the Sequel II System ...
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bioRxiv - Genomics 2023Quote: ... Lysates were sonicated for 3 cycles using Bioruptor Pico (Diagenode) and dialyzed against RIPA buffer for 3 hours at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... sonicated a minimum of 3 x 30 seconds (Diagenode sonicator), were optionally reduced with 100 mM dithiothreitol ...
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bioRxiv - Genomics 2024Quote: ... HMW DNA was sheared with Megaruptor 3 (Diagenode, B06010003 & E07010003) using settings 28/31 and used to generate PacBio HiFi libraries via the SMRTbell Prep Kit 3.0 (PacBio ...
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bioRxiv - Genetics 2021Quote: ... 3 to 7 days later individual colonies were picked and expanded and western blot was used to confirm Cas9 protein expression (Diagenode, C15200203). Cas9 expression in a high expressing line was confirmed by immunohistochemistry (5).
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bioRxiv - Systems Biology 2023Quote: ... 100 mM Tris-HCl pH 8.5) and boiled for 7 min at 95 °C before high-energy sonication to shear DNA (Diagenode Bioruptor pico). Protein concentrations were determined via a BCA assay (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... and sonication bath (3×10 sec at 4°C) (Bioruptor, Diagenode). Lysates were centrifuged (14.000x g ...
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bioRxiv - Genomics 2022Quote: ... We sheared the genomic DNA with the Megaruptor® 3 (Diagenode) and purified them using AMPure PB magnetic beads (Pacific Biosciences ...
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bioRxiv - Genomics 2023Quote: ... cerevisiae DNA S288C were sheared using a Megaruptor 3 (Diagenode, NJ); aliquot 1 ...
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bioRxiv - Microbiology 2023Quote: High molecular weight gDNA was sheared with the Megaruptor 3 (Diagenode) to obtain 10- 15 kbp fragments ...
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bioRxiv - Microbiology 2019Quote: ... 30 seconds off) for 7 cycles of sonication in a 4°C water bath using the Bioruptor Sonication System (Diagenode, Denville, NJ) at the high-intensity setting ...
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bioRxiv - Molecular Biology 2021Quote: ... and sonicated using a Bioruptor (Diagenode, medium power, 3 x 15 sec). Protein concentration was measured using the Bradford assay (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The DNA was sheared twice with the Megaruptor® 3 from Diagenode with a velocity setting of 30 and 31 ...
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bioRxiv - Genomics 2024Quote: ... High molecular weight genomic DNA was sheared mechanically on Megaruptor 3 (Diagenode) to generate 30 Kb fragments ...
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bioRxiv - Cell Biology 2020Quote: ... The dry samples were resuspended in 0.8 ml of 0.5% (v/v) trifluoroacetic acid (TFA) solution in HPLC-grade water with the assistance of sonication (Bioruptor Plus, Diagenode, 5 cycles of 30 s ON ...
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bioRxiv - Genomics 2019Quote: ... 3 µg of genomic DNA from each sample were fragmented using a Bioruptor (Diagenode), purified ...
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bioRxiv - Neuroscience 2021Quote: ... sonicated for 3×5 minutes in a 4°C Bioruptor ultrasonicator (UCD-200, Diagenode), vortexed ...
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bioRxiv - Cell Biology 2021Quote: ... To shear any remaining nucleic acids, samples were sonicated in high mode (30 sec OFF, 30 sec ON) for 12 cycles (Bioruptor® Plus; Diagenode). Then ...
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bioRxiv - Genomics 2023Quote: DNA was sheared to an appropriate size range (10-20 kb) using Megaruptor 3 (Diagenode) followed by Ampure bead purification ...
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bioRxiv - Genomics 2023Quote: DNA was sheared to an appropriate size range (10-20 kb) using Megaruptor 3 (Diagenode) followed by SMRTbell cleanup beads ...
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bioRxiv - Genomics 2023Quote: ... 12 μg of DNA was prepared for sequencing using Megaruptor 3 shearing (Diagenode, settings 19/31) and the Express Template Prep Kit v2 and SMRTbell Cleanup Kit v2 (PacBio) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2–5.5 µg of each sample was sheared using the Megaruptor 3 instrument (Diagenode, Liege, Belgium) at 18-20ng/µl and speed setting 31 ...
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bioRxiv - Neuroscience 2024Quote: ... The lysates were then sonicated with 3 × 10 cycles (30 s on and off) (Bioruptor, Diagenode) to generate chromatin fragments of ∼500 bp in length ...
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bioRxiv - Genomics 2020Quote: ... The latter aliquot was processed for ChIP using 3 μg anti-H3K4me3 antibody (pAb-003-050, Diagenode) as indicated in the user manual ...
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bioRxiv - Genetics 2020Quote: ... 30 s ON/30 s OFF for 15 min × 3 times in Bioruptor Sonication System UCD200 (Diagenode) in 15 ml polystyrene conical tubes ...
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bioRxiv - Genomics 2020Quote: ... the DNA was sheared to between 15 kb and 23 kb using the Megaruptor® 3 (Diagenode). HiFi sequencing libraries were prepared28 using SMRTbell™ Express Template Prep Kit 2.0 and followed by immediate treatment with the Enzyme Clean Up Kit (PN ...
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bioRxiv - Molecular Biology 2021Quote: ... then the sample was sonicated at a high intensity (3 × 5 min) using a Bioruptor sonicator (Diagenode). To obtain organoid-MPF cocultures ...
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bioRxiv - Cancer Biology 2023Quote: ... The pellets were resuspended in 300 μL Lysis Buffer 3 and sonicated by a Bioruptor Pico (Diagenode), resulting in chromatin fragments of around 300 to 500 bps ...
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bioRxiv - Genomics 2024Quote: ... HMW gDNA was sheared to 15-18 Kb using Diagenode’s Megaruptor 3 system (Diagenode, Belgium; cat. B06010003). The sheared gDNA was concentrated using 1x of SMRTbell cleanup beads for the repair and a-tailing incubation at 37°C for 30 minutes and 65°C for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Pellets were resuspended in 1.8mL of lysis buffer 3 (10mM Tris-HCl pH 7.5, 100mM NaCl, 1mM EDTA) and split in 6x 1.5mL sonication tubes (Diagenode). Upon 8 cycles of sonication with Pico Bioruptor (Diagenode ...
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bioRxiv - Cancer Biology 2021Quote: ... and sonicated (3 cycles of the 30 sec pulse with 30 sec interval on ice) using Bioruptor (Diagenode). The mixture was then boiled at 95 °C for 5 min to denature the proteins ...
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bioRxiv - Genomics 2023Quote: 10 ug of C5 genomic DNA was sheared to 30 kb - 50 kb using the Megaruptor 3 (Diagenode). The sheared DNA was treated with exonuclease to remove single-stranded ends ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...