Labshake search
Citations for Diagenode :
1 - 50 of 56 citations for 7 Azaspiro 3.4 octane 6 8 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... Upon 8 cycles of sonication with Pico Bioruptor (Diagenode) (30sec ON/ 30sec OFF) ...
-
bioRxiv - Genomics 2023Quote: Two micrograms of genomic DNA were sonicated for 6 cycles (6 s on, 90 s off) at 4 °C with a Bioruptor NGS (Diagenode), generating average fragments of 1 kb ...
-
bioRxiv - Genomics 2020Quote: ... Genomic DNA was sheared to 8 kb fragments using Megaruptor 2 (Diagenode). Standard SMRTbell libraries were prepared using Template Preparation Kit 1.0 (Pacific Biosciences ...
-
bioRxiv - Neuroscience 2022Quote: ... using 7 cycles for chromatin shearing on a Bioruptor Pico sonicator (Diagenode, Cat. No. B01060001). Prior to sonication ...
-
bioRxiv - Neuroscience 2022Quote: ... Lysates were sonicated with a Bioruptor Pico sonicator (Diagenode, total time 8 min) and 4μg of antibodies were added to sheared chromatin (from 4 million cells for Olig2 and from 1 million cells for histone marks ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1mM EDTA) and subjected to 8 cycles of sonication with Pico Bioruptor (Diagenode) (30sec ON/ 30sec OFF) ...
-
bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13.000 rpm and 4 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13,000 rpm and 4 °C ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... approximately 7 µg of DNA was sheared at 20 kb using the megaruptor system (Diagenode, Liege, Belgium). A DNA damage repair step was performed on 5 µg of DNA ...
-
bioRxiv - Molecular Biology 2024Quote: ... The DNA was sheared by sonication (4x 7 cycles, 30s on / 30s off, power high; Bioruptor, Diagenode) at 4°C and lysates cleared by centrifugation at 16,100 x g at 4°C for 15 min ...
-
bioRxiv - Genomics 2020Quote: ... 5 μg of purified DNA was sheared at 8 kb using the megaruptor1 system (Diagenode), followed by a DNA damage repair step on 2 μg of sample ...
-
bioRxiv - Cancer Biology 2021Quote: ... Chromatin corresponding to 8 μg of DNA was incubated with anti-H3K9me3 (Diagenode; Cat#: C15410193), anti-H3K27me3 (Diagenode ...
-
bioRxiv - Immunology 2022Quote: ... Sonicated chromatin was diluted ten times in ChIP Dilution Buffer and immunoprecipitation was done overnight at 4°C by incubating 2.5 μl (7 μg) of anti-H3K27ac antibody (C15410196; Diagenode) precoated on 25-μl protein A-coated magnetic beads (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2023Quote: ... After this time, samples were sonicated twice (7 cycles 30s on, 30s off) with a Bioruptor Plus sonicator (Diagenode). In between the two sonication cycles samples were incubated at 12°C with 0.8ul of Benzonase (Sigma) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The chromatin samples were sonicated for 7 cycles (30 s ON, 30 sec OFF, at High output) in a Bioruptor (Diagenode). The samples were heated to 95°C for 5 min and stored at -20°C ...
-
bioRxiv - Genetics 2021Quote: ... 3 to 7 days later individual colonies were picked and expanded and western blot was used to confirm Cas9 protein expression (Diagenode, C15200203). Cas9 expression in a high expressing line was confirmed by immunohistochemistry (5).
-
bioRxiv - Systems Biology 2023Quote: ... 100 mM Tris-HCl pH 8.5) and boiled for 7 min at 95 °C before high-energy sonication to shear DNA (Diagenode Bioruptor pico). Protein concentrations were determined via a BCA assay (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2021Quote: ... for 6 cycles (1 min on/1 min off) on a Bioruptor Pico sonicator (Diagenode). For each ChIP reaction 4 % HEK293T-derived human spike-in lysate was combined with mESC lysate and incubated in 1x IP buffer (50 mM HEPES/KOH pH 7.5 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were sonicated in 6 x 30 second intervals using a Bioruptor 300 Machine (Diagenode), then centrifuged for 10 minutes at 10,000g at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... 30 seconds off) for 7 cycles of sonication in a 4°C water bath using the Bioruptor Sonication System (Diagenode, Denville, NJ) at the high-intensity setting ...
-
bioRxiv - Biophysics 2024Quote: ... for 6 cycles (1 min on and 1 min off) on a Bioruptor Pico sonicator (Diagenode). ChIP lysates equivalent to 50 ug DNA were incubated in 1x IP buffer (50 mM HEPES/KOH pH 7.5 ...
-
bioRxiv - Genomics 2021Quote: ... 8 µg of adequately sheared chromatin was subjected to immunoprecipitation using iDeal ChIP-seq kit for Histones (Diagenode, C01010050) with 2 µg of antibodies against histone modifications or 10 µg of custom RPB1 antibody ...
-
bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
-
bioRxiv - Biochemistry 2023Quote: ... and the air-dried pellets were resuspended in 500 μl 8 M urea and sonicated in a bioruptor (Diagenode) with high amplitude for 10 min with cycles of 30 seconds on and 30 seconds off.
-
bioRxiv - Genomics 2023Quote: ... 25 ng of RNA in 8 µL water was input to the D-Plex Small RNA kit (Diagenode, C0503000) and libraries were prepared according to the manufacturer recommendations with the exception that 9 cycles of PCR were carried out ...
-
bioRxiv - Molecular Biology 2019Quote: ... Samples were sonicated with 8 cycles of 30s with 30s intervals at the low setting in a Bioruptor Plus water bath sonicator (Diagenode) and centrifuged for 15 min at 16000 g at 4°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... treated by RNase A (20 µg/mL) for 1h at 37°C in TE buffer and sonicated 8 min with BioRuptor (Diagenode) (15 sec ON / 15 sec OFF ...
-
bioRxiv - Physiology 2020Quote: ... Resulting nuclei were centrifuged at 12,000 RPM and resuspended in shearing buffer (1% SDS, 10mM EDTA pH 8, 50mM Tris pH8) and sheared in 1.5ml Bioruptor Microtubes (Diagenode, C30010016) in a Bioruptor (Diagenode ...
-
bioRxiv - Molecular Biology 2019Quote: ... mixed by rotation at 4°C for 30 min, and then sonicated for 8 cycles (ON for 30 s, OFF for 30 s) using a Picoruptor Sonicator (Diagenode). Each tube of lysate was incubated overnight at 37°C in hybridization buffer (750 mM NaCl ...
-
bioRxiv - Molecular Biology 2021Quote: ... ChIP from S2 cells was carried out using the same protocol from 2 x 107 cells with a modification to the chromatin preparation procedure to sonicate chromatin for 8 cycles of 30s on/30s off using a Bioruptor Pico (Diagenode). Recovered DNA was quantified on an Agilent TapeStation System using a High Sensitivity DNA ScreenTape (Agilent) ...
-
bioRxiv - Microbiology 2019Quote: ... washed cell pellets were lysed in 50 mM HEPES pH 8 with 5% SDS followed by 10 min (30 sec on/off) sonication in a Bioruptor Pico sonicator (Diagenode) at 18 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... The nuclei were suspended in the shearing buffer and sonicated for 8 cycles of 30sec on/off using Bioruptor Pico (Diagenode). The sheared chromatin was reverse-crosslinked and DNA fragment size was ensured by Agilent TapeStation ...
-
bioRxiv - Cell Biology 2023Quote: ... and lysed by sonication at high power for 8 cycles (15 sec on, 60 sec off) using a Bioruptor (Diagenode). The lysates were collected after centrifugation at 13,000 rpm for 10 min at 4 °C and the supernatants were prepared for nuclear protein analysis ...
-
bioRxiv - Neuroscience 2023Quote: ... and 8 μl was used for small RNA libraries construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). Indexes were attached using the D-Plex Single Indexes for Illumina - Set A (Diagenode C05030010 ...
-
bioRxiv - Developmental Biology 2024Quote: ... were sonicated 8 pulses (30 seconds ON / 30 seconds OFF) to 200–500 bp fragments using a Bioruptor Pico sonicator (Diagenode) in 200ul of L3 buffer ...
-
bioRxiv - Immunology 2024Quote: ... Nuclear extracts were prepared 21 and chromatin DNA was sonicated using Bioruptor Pico (Diagenode, 8 cycles, 30s on/off bursts) to generate DNA fragments ∼200-700 bp ...
-
bioRxiv - Developmental Biology 2019Quote: ... Homogenates were sonicated for 6 cycles of 30 seconds ON/30 seconds OFF on a PicoBioruptor (Diagenode), centrifuged for 10 minutes at 16,000 g at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... was added to the lysate and incubated at 37 °C for 10 min before sonication (2 × 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
-
bioRxiv - Microbiology 2022Quote: ... were added to the lysate and incubated at 37 °C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
-
bioRxiv - Biochemistry 2022Quote: ... Crosslinked proteins were denatured in 8 M urea 50 mM Tris and sonicated for 15 cycles of 30 s with a Bioruptor Plus (Diagenode, SA) at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... were added to the lysate and incubated at 37°C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
-
bioRxiv - Genomics 2023Quote: ... and sonicated for 6 cycles of 30s ON/30s OFF on high power using the Bioruptor Plus (Diagenode). Insoluble material was removed by centrifugation at >16,000g for 10 min at 4°C ...
-
bioRxiv - Cell Biology 2019Quote: ... or Raji iBZLF1 cells induced for 6 h were lysed in RIPA buffer and sheared with the BioRuptor (Diagenode) four times (5 min ...
-
bioRxiv - Plant Biology 2020Quote: ... samples were placed on ice for 10 min and sonicated for 6 cycles 15 sec “on” – 15 sec “off” using Bioruptor Plus (Diagenode). After that ...
-
bioRxiv - Genomics 2022Quote: ... Cell lysis was facilitated by passing the lysates through 26G needle 10 times during the incubation on ice, and by 6 cycles of sonication (30 sec “on”/30 sec “off”, low input) on a Bioruptor Pico (Diagenode). Lysates were then treated with 40 U DNase I (Worthington ...
-
bioRxiv - Microbiology 2023Quote: ... DNA was sonicated at 4°C (program: 6 cycles of 30 s on, 60 s off) on a Bioruptor Plus (Diagenode), achieving a size distribution between 200-1000 nucleotides (nt) ...
-
bioRxiv - Molecular Biology 2023Quote: ... chromatin was sheared by sonication into 500 – 1000pb fragment (30sec ON/30sec OFF, 6 cycles, 4°C, Diagenode Bioruptor Pico). 10µl was kept aside as the input ...
-
bioRxiv - Molecular Biology 2024Quote: ... and sonicated (6 cycles, high power for 30 s on and 30 s off at 4 °C, Bioruptor NextGen (Diagenode)) ...
-
bioRxiv - Molecular Biology 2019Quote: ... added (Figure 5B-5D) or 2x 300 µl was sonicated in 2x 1.5 ml bioruptor pico microtubes (Diagenode, Figure 6-9). When using the APLP protease inhibitors ...
-
bioRxiv - Biochemistry 2021Quote: ... Lysates were first sonicated for 6 cycles of 30 sec on/30 sec off under low power setting with Bioruptor™ Twin (Diagenode), and then centrifuged at 17,000 × g for 10 min at 4 °C ...