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Citations for Diagenode :
151 - 200 of 429 citations for 7 AMINO 2 TERT BUTOXYCARBONYL 1 2 3 4 TETRAHYDROISOQUINOLINE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... were added to the lysate and incubated at 37 °C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Biochemistry 2022Quote: ... heated to 95°C for 10 min shaking (1000 rpm) followed by 2 cycles à 20 s of sonication in BioRuptor (Diagenode). Aliquots containing 50 μg protein were transferred into a cartridge and cooled ...
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bioRxiv - Genomics 2022Quote: ... HMW-DNA (25 ng/μl) was separately sheared with 30 kb and 35 kb settings using a Megaruptor 2 instrument (Diagenode SA). However ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 2 ug of genomic DNA was fragmented into 200-300 bp using the Bioruptor UCD-200 sonicator (Diagenode, Denville, NJ). Libraries were constructed using the DNA library prep kit for BGI platform (BGI ...
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bioRxiv - Microbiology 2024Quote: ... were added to the lysate and incubated at 37°C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 50 ng/μl and sheared to 6 Kb fragments using Megaruptor® 2 (Diagenode, Seraing, Belgium). Shearing quality was estimated with TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... 600 ng of denatured DNA was incubated with 2 mg of 5-methylcytosine (5-mC) monoclonal antibody ([33D3] Diagenode, Cat No # C15200081) in IP buffer (0.1% SDS ...
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bioRxiv - Genomics 2021Quote: ... Abcam; anti-ERG 2 µg, ab92513, Abcam; anti-H3K4me3 1.4 µg, C15410003, Diagenode; H3K27me3 2.9 µg, C15410069, Diagenode; H3K27ac 1µg, ab4729, Abcam). As wildtype FLI1 or ERG are not expressed in EWSR1-FLI1 or EWSR1-ERG positive EwS cell lines ...
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bioRxiv - Neuroscience 2022Quote: ... using 7 cycles for chromatin shearing on a Bioruptor Pico sonicator (Diagenode, Cat. No. B01060001). Prior to sonication ...
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bioRxiv - Genetics 2021Quote: ChlPseq was performed on 20k FACS sorted MM cells as previously described 44 using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) but with minor modifications on the preparation of input controls ...
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bioRxiv - Genomics 2020Quote: Preparation of reagents: ChIP Wash buffers 1 to 4 (from iDeal ChIP-seq kit for histones, Diagenode C01010173) are aliquoted into quarter module reservoirs divided by length ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13,000 rpm and 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13.000 rpm and 4 °C ...
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bioRxiv - Genomics 2022Quote: ... and 12 μg of high molecular weight gDNA was sheared to a size distribution between 15 and 20 kbp using the Megaruptor® 2 (Diagenode, Denville, NJ, USA). For each accession ...
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bioRxiv - Plant Biology 2021Quote: ... Nucleic acids were fragmented with a Diagenode Bioruptor® Sonicator (Diagenode, Belgium). For immunoprecipitation ...
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bioRxiv - Evolutionary Biology 2019Quote: ... approximately 7 µg of DNA was sheared at 20 kb using the megaruptor system (Diagenode, Liege, Belgium). A DNA damage repair step was performed on 5 µg of DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... The DNA was sheared by sonication (4x 7 cycles, 30s on / 30s off, power high; Bioruptor, Diagenode) at 4°C and lysates cleared by centrifugation at 16,100 x g at 4°C for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... Overnight incubation with primary antibodies at 4°C was performed using the following antibodies: Cas9 (Diagenode, C15200230-100, 1:500), alpha synuclein (Abcam ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Genetics 2023Quote: ... or 4 µg H3K9me3 antibody (C15410193, Diagenode) coupled to 30µL beads was added to 400µL soluble chromatin (Diagenode H3K9me3 antibody was used in Figure 2E only ...
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bioRxiv - Molecular Biology 2023Quote: ... After this time, samples were sonicated twice (7 cycles 30s on, 30s off) with a Bioruptor Plus sonicator (Diagenode). In between the two sonication cycles samples were incubated at 12°C with 0.8ul of Benzonase (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Plant Biology 2023Quote: ... Slides were blocked with 4% BSA for 30 minutes at 37°C in a moist box followed by primary (anti-H3K9m2, C15410060, Diagenode, 1:500) and secondary (Alexa Fluor 488 Goat anti-Rabbit IgG1 Secondary Antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin samples were sonicated for 7 cycles (30 s ON, 30 sec OFF, at High output) in a Bioruptor (Diagenode). The samples were heated to 95°C for 5 min and stored at -20°C ...
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bioRxiv - Genetics 2023Quote: ... Briefly, DNA from activated B cells (day 4 with IL-4, anti-CD40) was sonicated to ∼1000 bp (Diagenode Bioruptor) before amplification via an Sµ-specific biotinylated primer (5’/5BiosG/CAGACCTGGGAATGTATGGT-3’) ...
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bioRxiv - Microbiology 2020Quote: ... high power at 4°C (Bioruptor Plus, Diagenode)] ...
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bioRxiv - Genetics 2020Quote: ... Resuspended pellets were shaken for 10 minutes at 4°C and lysed by sonication in a 4°C water bath (Diagenode Biorupter) for five minutes (30 seconds on/30 seconds off) ...
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bioRxiv - Genetics 2021Quote: ... 3 to 7 days later individual colonies were picked and expanded and western blot was used to confirm Cas9 protein expression (Diagenode, C15200203). Cas9 expression in a high expressing line was confirmed by immunohistochemistry (5).
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bioRxiv - Systems Biology 2023Quote: ... 100 mM Tris-HCl pH 8.5) and boiled for 7 min at 95 °C before high-energy sonication to shear DNA (Diagenode Bioruptor pico). Protein concentrations were determined via a BCA assay (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... in a 4°C-cooled water bath sonicator (Diagenode Bioruptor ...
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bioRxiv - Physiology 2020Quote: ... and sonicated at 4 degrees using Bioruptor sonicator (Diagenode) for 20 cycles on high setting ...
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bioRxiv - Genomics 2021Quote: ... Per ChIP 4 µl RARα antibody (serum, Diagenode C15310155) or 2 µg IgG control (Diagenode C15410296 ...
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bioRxiv - Systems Biology 2020Quote: ... Pellets were sonicated at 4°C using a Bioruptor (Diagenode) to shear the chromatin to a mean length of 300 bp by repeated cycles (16 cycles of 30 s ON and 30 s OFF) ...
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bioRxiv - Immunology 2020Quote: ... 30 s OFF at 4°C using a Bioruptor (Diagenode) with intermittent quick vortex and centrifugation using polystyrene tubes ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were sonicated at 4°C in Bioraptor Pico (Diagenode) for 10 cycles ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 s on/30 s off at 4° C (Bioruptor, Diagenode). One tenth of the sample was taken as input control ...
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bioRxiv - Genomics 2019Quote: ... cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were sonicated at 4°C using the Bioruptor Sonicator (Diagenode), with 6 cycles of 10 seconds on and 10 seconds off ...
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bioRxiv - Bioengineering 2019Quote: ... 20 minute rotation at 4 °C followed by sonication (Bioruptor, Diagenode; two cycles on low power setting ...
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bioRxiv - Neuroscience 2019Quote: ... Neurons were lysed at 4°C with the Bioruptor Plus (Diagenode) by sonicating for 15 cycles of 30 sec ...
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bioRxiv - Neuroscience 2019Quote: Neurons were lysed at 4°C with the Bioruptor Plus (Diagenode) by sonicating for 10 cycles of 30 sec ...
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bioRxiv - Plant Biology 2019Quote: ... Isolated nuclei were sonicated at 4°C using Bioruptor® (Diagenode) at high intensity for 2 cycles (30 sec ON/30 sec OFF) ...
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bioRxiv - Biochemistry 2021Quote: ... protease inhibitor cocktail) and sonicated in 4°C using Bioruptor (Diagenode) until lysate is clear ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were sonicated at 4°C using Bioruptor Pico (Diagenode B01080010) on high mode ...
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bioRxiv - Cell Biology 2020Quote: ... The dry samples were resuspended in 0.8 ml of 0.5% (v/v) trifluoroacetic acid (TFA) solution in HPLC-grade water with the assistance of sonication (Bioruptor Plus, Diagenode, 5 cycles of 30 s ON ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were sonicated in a 4 ⁰C water bath ultrasonicator (Bioruptor, Diagenode). The time of sonication was adjusted to yield short DNA fragments <500 bp (total 8 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were sonicated at 4 °C in a Bioruptor Waterbath Sonicator (Diagenode) for 5 cycles (30 sec on ...
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bioRxiv - Cell Biology 2021Quote: ... then sonicated at 4 °C in a Bioruptor Twin sonicating device (Diagenode) on HIGH 30 sec ON ...
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bioRxiv - Genomics 2021Quote: ... sonicated for 10 cycles at 4°C using BioruptorPico device from Diagenode. For immunoprecipitation of H3K4me1 ...