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Citations for Diagenode :
151 - 200 of 307 citations for 7 CHLOROMETHYL 2 2 DIMETHYL 2 3 DIHYDRO 1 BENZOFURAN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... we obtained 5 μg of high molecular weight genomic DNA (> 50% of fragments ≥ 40 kb) and sheared fragments to ~10 kb using the Megaruptor 3 (B06010003; Diagenode), followed by DNA repair and ligation of PacBio adapters using the SMRTbell Template Prep Kit v1.0 (100-991-900) ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation at 95C for 5 min the sample was sonicated 3 times for 30 sec on/off with a Bioruptor (Diagenode) on high power ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... in the presence of protease inhibitors and subjected to 3 × 10 cycles (30 seconds on and 30 seconds off) of sonication (Bioruptor, Diagenode) to generate chromatin fragments of ∼300 bp in length ...
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bioRxiv - Molecular Biology 2021Quote: ... the nuclear lysate was sonicated for 3 cycles of 30 seconds ON / 30 seconds OFF using a Bioruptor Pico (Diagenode) to ensure solubilisation of chromatin and then spun 18,000g for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... the remaining pellet was resuspended in 50μl RIPA supplemented with 1x Protease Inhibitor Cocktail and sonicated for 3-5 min with Picoruptor® (Diagenode). 10μg of proteins were then separated through precast 4-12% NuPAGE® Bis-Tris gels (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were sonicated briefly (3 cycles, 12 s on, 12 s off) at a high setting in a Bioruptor (Diagenode) sonicator ...
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bioRxiv - Genomics 2023Quote: ... Additional samples HG02554 and HG02953 were prepared for sequencing in the same way but with the following modifications: isolated DNA was sheared using the Megaruptor 3 instrument (Diagenode) twice using settings 31 and 32 to achieve a peak size of ∼15-20 kbp ...
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bioRxiv - Genomics 2023Quote: 15 μg of DNA was cleaned with a 1X AMPure PB beads (Pacific Biosciences) cleanup and sheared to a target size of 14 kilobases (kb) using the Megaruptor 3 instrument (Diagenode) with the following settings ...
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bioRxiv - Molecular Biology 2023Quote: ... 2mM EDTA) and briefly sonicated to remove flagella (ON 5 sec – OFF 30 sec x 3 Cycles, bioruptor Pico, Diagenode). The samples were transferred in low retention tubes ...
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bioRxiv - Genomics 2024Quote: HMW DNA was sheared to 20 kb using Covaris G-Tubes (for shallow-depth sequencing with MinION) or using the Megaruptor 3 (Diagenode; for high-depth sequencing with PromethION) ...
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bioRxiv - Plant Biology 2022Quote: ... A HiFi library was then prepared according to the “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” manual with an initial DNA fragmentation by Megaruptor 3 (Diagenode, Belgium) and final library size binning into defined fractions by SageELF (Sage Science ...
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bioRxiv - Cancer Biology 2024Quote: ... buffer with added protease and phosphatase inhibitors and sonicated on high for 30 seconds off 30 seconds on for 3 minutes (Diagenode Bioruptor). The protein concentration was determined using the Micro BCA TM protein assay kit (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... and subsequently sonicated for 3 cycles (30 seconds ON, 30 seconds OFF) on low settings using a Bioruptor® Plus (Diagenode, #B01020001). Samples were then centrifuged for 10 min at 10.000 g and supernatant was collected ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Genetics 2023Quote: ... H3K79me3 (1:1000; Diagenode, C15410068, or 1:1000; Abcam, ab2621), H3K27me3 (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ChIP assays were performed on leaves and axillary buds enriched samples of 3 weeks old plants using anti-H3K27me3 (Diagenode pAb-195-050) and anti-IgG (Abcam ab6702) ...
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bioRxiv - Genomics 2021Quote: ... H3K27me3 (1:100, C15410069, Diagenode), and SOX9 (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... H3K9me3 (Diagenode; #C15410193; 1:1000) and H3K27me3 (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... H4K20me1 (Diagenode; #C15410034; 1:500), H3K9me3 (Diagenode ...
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bioRxiv - Developmental Biology 2023Quote: ... H3K4me3 (Diagenode #C15410003, 1 μg), H3K27me3 (Diagenode #C15410069 ...
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bioRxiv - Cell Biology 2023Quote: ... H3K4me3 (1:1000; Diagenode C15410003), H3S10ph clone MC463 (1:2000 ...
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bioRxiv - Genetics 2023Quote: ... H3K79me1 (1:1000; Diagenode, C15410082), H3K79me2 (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sox4 (1:4000, Diagenode #C15310129), GAPDH (1:4000 ...
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bioRxiv - Neuroscience 2024Quote: ... H3K27me3 (Diagenode, C1541095, 1:2000), Total H3 (Cell Signaling ...
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bioRxiv - Molecular Biology 2021Quote: ... for 6 cycles (1 min on/1 min off) on a Bioruptor Pico sonicator (Diagenode). For each ChIP reaction 4 % HEK293T-derived human spike-in lysate was combined with mESC lysate and incubated in 1x IP buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... for 6 cycles (1 min on and 1 min off) on a Bioruptor Pico sonicator (Diagenode). ChIP lysates equivalent to 50 ug DNA were incubated in 1x IP buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... and H3K27me3 (Diagenode; #C15410195; 1:200). After washing ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-H3K36me3 (1:50, Diagenode C15200183), anti-H3K9me3 (1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-CTCF (1:400, Diagenode C15410210), anti-SMC1 (1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-H3K27ac (1:100, Diagenode C15410196), anti-H3K36me2 (1:50 ...
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bioRxiv - Plant Biology 2021Quote: ... anti-GT1 (1:75) and anti-YSPTSPS repeat S2Pho (RNA Pol II, B1 subunit; 1:200, Diagenode). The samples were washed for 8h at 4°C with gentle agitation with PBS (0.2% v/v Tween-20 ...
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bioRxiv - Neuroscience 2020Quote: ... H3K4me3 (Diagenode C15410003-50; rabbit; 1 µg), H3K27ac (Abcam ab177178 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Ty1 antibody (Diagenode, #C15200054, 1:3000) and Peroxidase AffiniPure donkey anti-rabbit IgG (Jackson ImmunoResearch 711-035-152 ...
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bioRxiv - Genomics 2020Quote: ... H3K27ac Antibody (C15410196, Diagenode, 1:600 ratio) were incubated with 40 µl of Dynabeads protein A/G (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... anti-H3K4me3 (1 μl/ChIP, C15410003 Diagenode) anti-H4K20me3 (2 μl/ChIP ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-H3K27me3 (Diagenode, dilution 1:1,000).
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bioRxiv - Cell Biology 2023Quote: ... 1:2000 C15200203 CRISPR/Cas9 7A9 (Diagenode), 1:1000 ab181602 GAPDH (Abcam)) ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed overnight at +4°C with primary antibodies (H3K4me2 1:1000; Abcam ab32356) (H3K4me3 1:200; Diagenode C15410003) (H3K4me2 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... H3K9me3 (Diagenode: Catalogue# pAb-056-050 1:1000) and H2AK119Ub (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... Rabbit anti-Cas9 antibody (Diagenode, C15310258, 1:1000), Mouse anti-β actin antibody (HRP conjugated ...
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bioRxiv - Genomics 2020Quote: ... rabbit polyclonal anti-H3K27me3 (1:1000; Diagenode C15410069); mouse monoclonal anti-p21 (1:500 ...
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bioRxiv - Bioengineering 2019Quote: ... Anti-Cas9 antibody (Diagenode, C15200203, 1:1000 dilution), and HRP-conjugated anti-mouse antibody (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... and mouse anti-pSer2 RNAPII (Diagenode, 1:1000). The secondary antibodies used were Cy2-donkey anti-rabbit ...
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bioRxiv - Developmental Biology 2021Quote: ... or mouse anti-H3 (Diagenode; C15200011; 1/1000) as primary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used: anti-Cas9 (Diagenode, C15200229, 1:10,000) and anti-ERK (Cell Signaling Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-H3K4me3 (Diagenode, C15410003-50; 1:300). Immunocomplexes were captures using Protein-A Sepharose beads CL-4B (GE Healthcare) ...
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bioRxiv - Genomics 2023Quote: ... rabbit polyclonal anti-H3K27me3 (1:1,000; Diagenode C15410069); rabbit polyclonal anti-p21 (1:500 ...
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bioRxiv - Developmental Biology 2019Quote: ... 2.5 μ 10% Triton X-100 and 1 l of anti-methylcytidine antibody (1 mg/ml Diagenode # MAb-081-μ 100) overnight ...