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Citations for Diagenode :
351 - 400 of 536 citations for 7 3S 5S 3 Amino 5 methyl 1 piperidinyl 1 cyclopropyl 1 4 dihydro 8 methoxy 4 oxo 3 quinolinecarboxylic acid with 2 hydroxybutanedioic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Lysates were sonicated with a Bioruptor Pico sonicator (Diagenode, total time 8 min) and 4μg of antibodies were added to sheared chromatin (from 4 million cells for Olig2 and from 1 million cells for histone marks ...
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bioRxiv - Cancer Biology 2022Quote: ... 1mM EDTA) and subjected to 8 cycles of sonication with Pico Bioruptor (Diagenode) (30sec ON/ 30sec OFF) ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13,000 rpm and 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13.000 rpm and 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 × 107 cells / immunoprecipitation were fixed for 30 minutes (min) at room temperature (RT) using Diagenode Crosslink Gold (Diagenode, Liège, Belgium). After two washing steps with PBS (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA (1 μg) was fragmented to 300–600 bp after 25 cycles of 30 s of pulsed sonication in Bioruptor (Diagenode, Belgium). Fragmented DNA was end-repaired and adapter ligation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were kept on ice and processed directly or frozen at −80C in 20 ml of RNAse/DNAse free PBS supplemented with 1× protein inhibitor (Diagenode, Belgium). Embryos younger than late gastrula (younger than E7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Each chromatin sample containing about 6,000 cells was incubated with 1 µg of anti-H3K27me3 antibody (Diagenode, C15410195, lot. A1811-001P). ChIP was performed using the automated platform SX-8G IP-Star platform (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... in 10 µl nuclease-free H2O and amplified with NEBNext® High-Fidelity 2X PCR Master Mix and 1 ul of primer pair from Diagenode (24 SI for tagmented libraries ...
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bioRxiv - Plant Biology 2023Quote: ... and cOmplete protease inhibitor cocktail tablets) and proceeded for sonication to shear the chromatin (∼ 500 bp to 1 kb size fragments) using Bioruptor Plus (Diagenode, USA) set at low mode and 45 cycles of 30 sec ON ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 min total (Diagenode).
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bioRxiv - Developmental Biology 2021Quote: ... 1 mM PMSF) and chromatin was sheared by sonication to approximately 500-100 bp fragments using the Bioruptor® sonicator (Diagenode, Belgium). Immunoprecipitation was done with the anti-H3K27me3 (Active-motif ...
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bioRxiv - Evolutionary Biology 2019Quote: ... approximately 7 µg of DNA was sheared at 20 kb using the megaruptor system (Diagenode, Liege, Belgium). A DNA damage repair step was performed on 5 µg of DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... The DNA was sheared by sonication (4x 7 cycles, 30s on / 30s off, power high; Bioruptor, Diagenode) at 4°C and lysates cleared by centrifugation at 16,100 x g at 4°C for 15 min ...
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bioRxiv - Genomics 2020Quote: ... C15200211 (Diagenode, 5 μl/ChIP); H3K4me1 ...
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bioRxiv - Genomics 2022Quote: ... libraries were constructed with 200 ng input DNA from each sample (n = 24; Table 1) using the Premium RRBS Kit (Diagenode, Cat. No. C02030032) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... PFFs were diluted in 1× PBS to a final concentration of 100 μL/Ml and sonicated with Bioruptor sonication device (Diagenode, Liege, Belgium, #B01020001) at the following settings ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin corresponding to 8 μg of DNA was incubated with anti-H3K9me3 (Diagenode; Cat#: C15410193), anti-H3K27me3 (Diagenode ...
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bioRxiv - Cancer Biology 2021Quote: ... sonicated 5 minutes (Bioruptor plus, Diagenode, 5 cycles high, 30 s on, 30 s off) and incubated at 37 °C for 60 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Genomics 2023Quote: ... a Megaruptor 2 (Diagenode) was used to shear 3 μg of DNA to 15kb fragments ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were crosslinked with 1% PFA at room temperature for 10 minutes and sonicated for 11 cycles (30 s ON/OFF, Diagenode Bioruptor Pico bath sonicator). 5% of the lysate was reserved as Input ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS and 0.5% N-lauroylsarcosine) and the chromatin was fragmented by sonication for 10 cycles on Bioruptor (Diagenode high settings, 30’’ on/off). Chromatin was separated from cell debris using centrifugation and stored at -80C for maximum of 2 months.
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bioRxiv - Molecular Biology 2023Quote: ... After this time, samples were sonicated twice (7 cycles 30s on, 30s off) with a Bioruptor Plus sonicator (Diagenode). In between the two sonication cycles samples were incubated at 12°C with 0.8ul of Benzonase (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... for 5 min (Bioruptor Sonicator, Diagenode)73–75 ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin samples were sonicated for 7 cycles (30 s ON, 30 sec OFF, at High output) in a Bioruptor (Diagenode). The samples were heated to 95°C for 5 min and stored at -20°C ...
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bioRxiv - Cell Biology 2023Quote: ... H3K27me3 (Diagenode; #C15410195; 2 μg) and IgG-rabbit (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... H3K9me3 (Diagenode; #C15410193; 2 μg), H3K27me3 (Diagenode ...
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bioRxiv - Molecular Biology 2019Quote: ... About 5 μg Ty1 antibody (C15200054; Diagenode), mouse IgG (12-371 ...
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bioRxiv - Genetics 2021Quote: ... 3 to 7 days later individual colonies were picked and expanded and western blot was used to confirm Cas9 protein expression (Diagenode, C15200203). Cas9 expression in a high expressing line was confirmed by immunohistochemistry (5).
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bioRxiv - Systems Biology 2023Quote: ... 100 mM Tris-HCl pH 8.5) and boiled for 7 min at 95 °C before high-energy sonication to shear DNA (Diagenode Bioruptor pico). Protein concentrations were determined via a BCA assay (Thermo Fisher) ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... 2 μg anti-H3K27me3 (Diagenode, C15410195), and 2.5 μg anti-enhancer of zeste homolog 2 (EZH2 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 µg of 5mC antibody (Diagenode) were added to the sample and incubated for 2 h at 4°C with rotation ...
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bioRxiv - Genomics 2022Quote: ... by boiling at 95°C for 5 minutes followed by 5 sonication cycles of 30 s ON / 30 s OFF on the Bioruptor Pico sonicator (Diagenode) at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 % SDS in PBS) and sonicated 5 times for 5 min (30s ON/ 30s OFF) at high mode in a Bioruptor Pico (Diagenode). IP were performed overnight at 4°C using 1.5 mg of pre-coated epoxy Epoxy Dynabeads (M-270 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 5 minutes with a Bioruptor Pico (Diagenode) to an average fragment size of ∼ 200 bp ...
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bioRxiv - Genomics 2021Quote: ... 8 µg of adequately sheared chromatin was subjected to immunoprecipitation using iDeal ChIP-seq kit for Histones (Diagenode, C01010050) with 2 µg of antibodies against histone modifications or 10 µg of custom RPB1 antibody ...
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bioRxiv - Biochemistry 2023Quote: ... and the air-dried pellets were resuspended in 500 μl 8 M urea and sonicated in a bioruptor (Diagenode) with high amplitude for 10 min with cycles of 30 seconds on and 30 seconds off.
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bioRxiv - Genomics 2023Quote: ... 25 ng of RNA in 8 µL water was input to the D-Plex Small RNA kit (Diagenode, C0503000) and libraries were prepared according to the manufacturer recommendations with the exception that 9 cycles of PCR were carried out ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein was extracted by incubating the islets with Laemmli buffer at 95°C for 5 mins and homogenized for another 5 mins in a sonication device (Diagenode, B01060001) at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... anti-H4K20me3 (2 μl/ChIP, C15410207, Diagenode), anti-PA28γ (0.5 μl /ChIP ...
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bioRxiv - Cell Biology 2020Quote: ... anti-H3K9me3 (2 μl/ChIP, C15410056 Diagenode), anti-H3K4me3 (1 μl/ChIP ...
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bioRxiv - Microbiology 2021Quote: ... gDNA was sheared using Megaruptor 2 (Diagenode) to an average size of 8.9kb ...
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bioRxiv - Genomics 2021Quote: ... or 2 µg IgG control (Diagenode C15410296) was used ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were sonicated with 8 cycles of 30s with 30s intervals at the low setting in a Bioruptor Plus water bath sonicator (Diagenode) and centrifuged for 15 min at 16000 g at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... treated by RNase A (20 µg/mL) for 1h at 37°C in TE buffer and sonicated 8 min with BioRuptor (Diagenode) (15 sec ON / 15 sec OFF ...
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bioRxiv - Molecular Biology 2019Quote: ... mixed by rotation at 4°C for 30 min, and then sonicated for 8 cycles (ON for 30 s, OFF for 30 s) using a Picoruptor Sonicator (Diagenode). Each tube of lysate was incubated overnight at 37°C in hybridization buffer (750 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... ChIP from S2 cells was carried out using the same protocol from 2 x 107 cells with a modification to the chromatin preparation procedure to sonicate chromatin for 8 cycles of 30s on/30s off using a Bioruptor Pico (Diagenode). Recovered DNA was quantified on an Agilent TapeStation System using a High Sensitivity DNA ScreenTape (Agilent) ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclei were suspended in the shearing buffer and sonicated for 8 cycles of 30sec on/off using Bioruptor Pico (Diagenode). The sheared chromatin was reverse-crosslinked and DNA fragment size was ensured by Agilent TapeStation ...