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Citations for Diagenode :
51 - 100 of 269 citations for 6 OXO 9α 11α 15S TRIHYDROXY PROSTA 13E 17Z DIEN 1 OIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Chromatin was sheared in Bioruptor® Pico 15 mL tubes with 300 µL beads (Diagenode C30010017) by 25 cycles of 30 sec on and 30 sec off using a Bioruptor® (Diagenode B01060010) ...
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bioRxiv - Microbiology 2023Quote: ... Purification steps were performed using AMPure PB beads (PacBio).15 μg of DNA was purified then sheared at 20 kb using the Megaruptor1 system (Diagenode). Using SMRTbell Express Template prep kit 2.0 ...
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bioRxiv - Cell Biology 2023Quote: ... for 15 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode; #C30010016). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... for 15 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode; #C30010016). Finally ...
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bioRxiv - Molecular Biology 2024Quote: ... cells lysates were sonicated (15 cycles: 10 sec. on/10 sec. off) using sonicator (Bioruptor, Diagenode) and centrifuged at 12,000 g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 sec off) for 15 minutes to shear chromatin to less than 500 bp with Bioruptor (Diagenode). After pelleting the insoluble fraction ...
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bioRxiv - Developmental Biology 2019Quote: ... DNA was crosslinked in formaldehyde and sonicated in a Bioruptor for 15 minutes at high power (Diagenode). Cells were resuspended in a total volume of 250 ml of Buffer A and Buffer B mix (half of the recommended volume for 0.5×106cells) ...
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bioRxiv - Plant Biology 2019Quote: ... The extracts were sonicated in 15 ml tubes in an ice bath using Bioruptor UCD-200 (Diagenode) on high setting ...
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bioRxiv - Genetics 2020Quote: ... 30 s ON/30 s OFF for 15 min × 3 times in Bioruptor Sonication System UCD200 (Diagenode) in 15 ml polystyrene conical tubes ...
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bioRxiv - Genomics 2020Quote: ... the DNA was sheared to between 15 kb and 23 kb using the Megaruptor® 3 (Diagenode). HiFi sequencing libraries were prepared28 using SMRTbell™ Express Template Prep Kit 2.0 and followed by immediate treatment with the Enzyme Clean Up Kit (PN ...
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bioRxiv - Genomics 2022Quote: A total of 15 μg of extracted genomic DNA was sheared using a Megaruptor (Diagenode, Denville, NJ). The large DNA fragment was recycled by BluePippin (Sage Science ...
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bioRxiv - Genomics 2022Quote: ... DNA was sheared for 26 cycles of shearing (15s on, 30s chilling) using a Bioruptor sonicator (Diagenode), then size selected using SPRI beads (Beckman ...
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bioRxiv - Plant Biology 2022Quote: ... Cells were ruptured by 5 × 15 sec sonication in an ice bath in Bioruptor UCD-200 (Diagenode) at medium intensity with 2 min breaks on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... polyA-selected mRNA was adjusted to 15 ng/µL in 100ul and fragmented using Bioruptor ultrasonicator (Diagenode) with 30s on/off for 30 cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations of lysates were determined after 15 min of sonication (Bioruptor, Diagenode, cycles of 30 s) using the Pierce BCA Protein Assay (Thermo) ...
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bioRxiv - Genomics 2024Quote: ... HMW gDNA was sheared to 15-18 Kb using Diagenode’s Megaruptor 3 system (Diagenode, Belgium; cat. B06010003). The sheared gDNA was concentrated using 1x of SMRTbell cleanup beads for the repair and a-tailing incubation at 37°C for 30 minutes and 65°C for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... and sonicated with 30 cycles of 15 s ON-OFF using a BioRuptor (Diagenode, Denville, NJ-USA). The EV lysate was reduced with 10 mmol/L dithiothreitol at 56 °C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... The Mock (15 ng in 20 µl TE buffer) was randomly fragmented by BioRuptor (Diagenode, Liege, Belgium) at default intensity for 15 sec on and 90 sec off ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sonicated in 6 x 30 second intervals using a Bioruptor 300 Machine (Diagenode), then centrifuged for 10 minutes at 10,000g at 4°C ...
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bioRxiv - Genetics 2020Quote: ... or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913, Diagenode #C15410193). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... and 10 µg of DNA were sheared to appropriate size range (15-20 kb) using Megaruptor 3 (Diagenode). The sheared DNA was concentrated by bead purification using PB Beads (PacBio) ...
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bioRxiv - Molecular Biology 2021Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Genomics 2020Quote: Total genomic DNA was sheared by sonication for 15–24 min using a Bioruptor (Diagenode, Inc., New Jersey, USA). Size-selected samples (200–400 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromatin was fragmented by sonication (30 cycles of 15 s ‘on’ and 30 s ‘off’) with a Bioruptor (Diagenode) to 200 – 1000 bp ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended samples were boiled for 5 min at 95°C and sonicated for 15 min in the Bioruptor (Diagenode) at 30 sec on ...
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bioRxiv - Genetics 2024Quote: ... Cells were lysed by sonication for 15 cycles (high, 30 seconds on, 30 seconds off) using Bioruptor Plus (Diagenode). After a 15-minute incubation on ice and centrifugation (21,000 ✕ g ...
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bioRxiv - Molecular Biology 2024Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Developmental Biology 2019Quote: ... Homogenates were sonicated for 6 cycles of 30 seconds ON/30 seconds OFF on a PicoBioruptor (Diagenode), centrifuged for 10 minutes at 16,000 g at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and protease inhibitor mix HP Plus (Serva)) for 15 min on ice and finally subjected to sonication using Bioruptor Plus sonication device (Diagenode). The lysed cells were centrifuged at 16,000 x g for 30 min at 4 °C and SDS sample buffer was added to the supernatant ...
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bioRxiv - Microbiology 2020Quote: ... supernatant aspirated and suspended in chromatin shearing buffer for sonication (10 cycles of 15 sec ‘on’ and 30 sec ‘off’) with a Bioruptor (Diagenode) to 200-900bp size ...
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bioRxiv - Immunology 2021Quote: ... Samples were split into 2 x 1.5 mL in 15 mL polypropylene tubes suitable for the Bioruptor® Pico (Diagenode). BioRuptor Sonicator settings ...
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bioRxiv - Molecular Biology 2020Quote: ... lysed with RIPA lysis buffer with protease inhibitors on ice for 15 minutes and then sonicated with a Bioruptor (Diagenode) for 10 minutes on high with 30 seconds on and 30 seconds off ...
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bioRxiv - Cell Biology 2022Quote: ... The cell lysate was transferred to 15 mL Bioruptor Pico tubes along with ~200μL of the corresponding sonication beads (Diagenode C010200031) and sonicated using a Bioruptor Pico (Diagenode B01060010 ...
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bioRxiv - Genomics 2022Quote: ... DNase I treatment was replaced by a step of chromatin shredding by sonication in an ice-cold bath (15 min, High, 45 sec ON/OFF, Diagenode). The GelFree fractionation was omitted to avoid loss of material because the eluate after the second purification step was judged sufficiently pure.
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bioRxiv - Molecular Biology 2020Quote: ... nucleic acids gently extracted from unfixed NTERA-2 cells were sheared by sonication (12 cycles at high power, 15s ON / 90s OFF) with a Bioruptor (Diagenode). 4.4 μg of sheared nucleic acids were incubated with 10 μg of S9.6 antibody for immunoprecipitation overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... The lysate was sonicated on ice at 30% amplitude for 3 minutes (15 seconds on 45 seconds off) followed by 40% amplitude for 30 seconds (15 seconds on 45 seconds off) in a Bioruptor (Diagenode). The sonicated lysate was supplemented with 1% NP-40 alternative and incubated rotating for 1 hour at 4C ...
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bioRxiv - Molecular Biology 2019Quote: ... Either the whole extract was sonicated in a 15 ml bioruptor pico tube with 300 µl of sonication beads (Diagenode) added (Figure 5B-5D ...
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bioRxiv - Molecular Biology 2019Quote: ... the WCL was fragmented as well by sonicating for 3 cycles 30 seconds on / 30 seconds off in 15 ml bioruptor pico tubes containing 300 µl of sonication beads (Diagenode). After sonication ...
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bioRxiv - Molecular Biology 2021Quote: ... vortexed briefly and sonicated for 10 cycles x (30’’ on /30’’ off, high setting condition) in 15 ml conical polystyrene tubes using a Bioruptor TM (Diagenode) sonicator with a 4°C water bath cold circulation system ...
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bioRxiv - Cell Biology 2020Quote: ... protease inhibitor cocktail), and sonicated 3x (15 sec on/30 sec off cycle, low power mode) in a Bioruptor (Diagenode). Nuclei were purified by sedimentation at 1,000 rpm for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... samples from multiple ChIP experiments were pooled and sonicated for 15 cycles (30 sec ON, 30 sec OFF, high setting) with a Bioruptor (Diagenode) then concentrated with a vacuum concentrator (Eppendorf) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10-15 cycles of sonication (30 sec ON, 30 sec OFF) were carried using the Bioruptor Pico sonicator device (Diagenode). H3k27ac antibody was obtained from Abcam (ab4729 ...
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bioRxiv - Neuroscience 2019Quote: ... followed by vortexing and sonication (20 cycles of 15 seconds ON/OFF, 10 minutes in total; Bioruptor plus (model UCD-300, Diagenode)) ...
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bioRxiv - Cell Biology 2020Quote: ... the lysate was diluted 10 times with lysis buffer and subjected to sonication for 15 min in a refrigerated water bath (Bioruptor, Diagenode). Part of the sonicated chromatin was treated with proteinase K and RNase A and analyzed by electrophoresis in a 1.5% agarose gel to control the size of the DNA fragments (200-500 bp) ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was spun at 13,000 rpm for 30 min at 4°C and the pellet was resuspended in 300 μl ChIP lysis buffer + 1mM PMSF and sonicated for 15 min (30 sec ON - 60 sec OFF) in a Bioruptor (Diagenode). The lysate was spun at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Genomics 2021Quote: ... Lysates were sonicated for 15 cycles (30 sec on / 30 sec off) at 4°C using a Bioruptor Pico sonicator (Diagenode). After sonication ...
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bioRxiv - Biophysics 2022Quote: ... samples were sonicated for 15 min (30 sec on, 30 sec off at 10°C) with a Bioruptor Pico (Diagenode). Then ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were sonicated using 40 cycles of 15s on/off at 4 °C in the Bioruptor plus (model UCD-300, Diagenode) after boiling at 99 °C for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then shaken again at RT for 5 minutes at 1000rpm before being sonicated for 15 cycles of 30 seconds on/ 30 seconds off with a BioRuptor (Diagenode). Benzonase was added to each sample and incubated at 37°C for 15 minutes to digest DNA ...
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bioRxiv - Cell Biology 2023Quote: ... and lysed by sonication at high power for 8 cycles (15 sec on, 60 sec off) using a Bioruptor (Diagenode). The lysates were collected after centrifugation at 13,000 rpm for 10 min at 4 °C and the supernatants were prepared for nuclear protein analysis ...