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Citations for Diagenode :
201 - 250 of 516 citations for 6 METHYL 4 4 NITRO PHENYL 2 OXO 1 2 3 4 TETRAHYDRO PYRIMIDINE 5 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... the remaining pellet was resuspended in 50μl RIPA supplemented with 1x Protease Inhibitor Cocktail and sonicated for 3-5 min with Picoruptor® (Diagenode). 10μg of proteins were then separated through precast 4-12% NuPAGE® Bis-Tris gels (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 2mM EDTA) and briefly sonicated to remove flagella (ON 5 sec – OFF 30 sec x 3 Cycles, bioruptor Pico, Diagenode). The samples were transferred in low retention tubes ...
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bioRxiv - Plant Biology 2019Quote: ... and sonicated 2 cycle x 30 second ON/ 30 second OFF using Bioruptor® Plus (Diagenode). After removal of cell debris by centrifugation (5 min ...
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bioRxiv - Microbiology 2023Quote: ... About 2 µg of genomic DNA was sheared by sonication with a Picoruptor (Diagenode, S.A., Belgium) instrument to a size of 300–400 bp in length ...
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bioRxiv - Genomics 2023Quote: ... 7μL Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Cell Signalling Technology #9733) or 1μg negative control rabbit IgG (Diagenode) per ChIP reaction ...
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bioRxiv - Developmental Biology 2019Quote: ... the amplified cDNA (2 μg per sample) was fragmented to 200 bp using a Bioruptor Sonicator (Diagenode), and barcode ligation and end repair were performed using the Ovation Rapid DR Library System (NuGEN) ...
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bioRxiv - Genetics 2020Quote: ... The DNA was sonicated for 2 cycles of 15sec ON/90 sec OFF in a bioruptor (Diagenode) at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and the nuclei were resuspended in 50 µL transposition mixture (25 µL 2× Tagmentation buffer (Diagenode, C01019043), 2.5 µL Tagmentase loaded (Diagnode ...
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bioRxiv - Systems Biology 2020Quote: ... Chromatin was broken into small pieces with an average size of 0.5-2 kb using the Bioruptor (Diagenode). The sonicated chromatin was then incubated with 5 µg of primary antibodies overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2019Quote: HMW genomic DNA was sheared to 30 kb using a Megaruptor® 2 instrument (Diagenode SA, Seraing, Belgium). DNA-damage and end repair was carried out prior to blunt adaptor ligation and exonuclease purification using ExoIII and ExoVII ...
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bioRxiv - Biochemistry 2021Quote: Cellular human and viral material in sputum deposited on glass slides was lysed in 50 μl 2% SDS dissolved in 50 mM Tris/HCl (pH 8.2) followed by sonication in a Bioruptor Pico (Diagenode). Proteins were digested using the SP3 protocol as described below.
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bioRxiv - Cell Biology 2021Quote: ... DNA was fragmented with 2 rounds of 30 cycles 30sec ON-30sec OFF of sonication with Bioruptor Plus (Diagenode) set on HIGH and purified with Qiagen MinElute kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was sheared to a modal size of 10 kb using a Megaruptor 2 (Diagenode Diagnostics, Seraing (Ougrée), Belgium) ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples were lysed the same day 2 weeks before proteomics analysis in 100µL iST-NHS-Lysis buffer (PreOmics GmbH) by sonication (Bioruptor (DIagenode) 15s ON/15s OFF for 15 min) ...
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bioRxiv - Immunology 2021Quote: ... Samples were split into 2 x 1.5 mL in 15 mL polypropylene tubes suitable for the Bioruptor® Pico (Diagenode). BioRuptor Sonicator settings ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed and sonicated in the presence of 0.2% SDS for 30 cycles (30 s on, 30 s off) using the high setting on a Bioruptor sonicator (Diagenode). Cell debris were pelleted by centrifugation and SDS in the lysate supernatants was diluted to 0.07% ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ChIPseq was performed as previously described (Bouderlique et al., 2019) using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) and the ThruPLEX DNA-seq (Rubicon Genomics ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were lysed for 15 min on ice with occasional inversion every 2 min and then sonicated for 24 cycles with 30s on and 30s off using Bioruptor (Diagenode). After sonication ...
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bioRxiv - Molecular Biology 2020Quote: ... nucleic acids gently extracted from unfixed NTERA-2 cells were sheared by sonication (12 cycles at high power, 15s ON / 90s OFF) with a Bioruptor (Diagenode). 4.4 μg of sheared nucleic acids were incubated with 10 μg of S9.6 antibody for immunoprecipitation overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Sonication was performed by splitting each sample to 2 Bioruptor tubes with sonication beads and using the Bioruptor Pico (Diagenode) for 18 cycles of 30 sec on and 30 sec off into an average size of approximately 200 bp ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was transferred to a sterile 2 ml microcentrifuge tube and sonicated with the Bioruptor UCD-200 sonicator (Diagenode) for 1 min on the low setting (160W at 20kHz ...
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bioRxiv - Neuroscience 2020Quote: ... the crosslinked cells were resuspended in 2 mL of lysis buffer and sonicated (in a cold room) by using a Bioruptor (Diagenode) with the following parameters ...
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bioRxiv - Genomics 2020Quote: ... high molecular weight DNA was diluted to a concentration of 25 ng/uL and fragmented to an average peak size of 25 kb using a Megaruptor-2 instrument (Diagenode). Small fragments <1 kb were removed and the DNA concentrated using bead purification (0.4 x volumes of Ampure-XP beads ...
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bioRxiv - Immunology 2021Quote: ... 2 µg of antibody was used per reaction. H3K9me3 (Lot. A2217P) and H3K4me3 (Lot No. A1052D) antibodies were from Diagenode. Immunoprecipitated and input DNA were extracted by phenol-chloroform extraction and used for qPCR with SsoAdvanced Universal SYBR Green supermix (Biorad) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using phenol/chloroform and 2 µg of purified 3C sample was sonicated using the Bioruptor Pico (Diagenode). Biotinylated DNA was captured using MyOne Streptavidin T1 magnetic beads (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... α-Synuclein PFFs were vortexed and diluted with DPBS to 2 mg/mL and sonicated in a cooled bath sonicator at 9°C (Diagenode Bioruptor® Pico ...
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bioRxiv - Microbiology 2020Quote: ... was added to the lysate and incubated at 37 °C for 10 min before sonication (2 × 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Microbiology 2022Quote: ... were added to the lysate and incubated at 37 °C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Biochemistry 2022Quote: ... heated to 95°C for 10 min shaking (1000 rpm) followed by 2 cycles à 20 s of sonication in BioRuptor (Diagenode). Aliquots containing 50 μg protein were transferred into a cartridge and cooled ...
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bioRxiv - Genomics 2022Quote: ... HMW-DNA (25 ng/μl) was separately sheared with 30 kb and 35 kb settings using a Megaruptor 2 instrument (Diagenode SA). However ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 2 ug of genomic DNA was fragmented into 200-300 bp using the Bioruptor UCD-200 sonicator (Diagenode, Denville, NJ). Libraries were constructed using the DNA library prep kit for BGI platform (BGI ...
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bioRxiv - Microbiology 2024Quote: ... were added to the lysate and incubated at 37°C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...
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bioRxiv - Genomics 2021Quote: ... Abcam; anti-ERG 2 µg, ab92513, Abcam; anti-H3K4me3 1.4 µg, C15410003, Diagenode; H3K27me3 2.9 µg, C15410069, Diagenode; H3K27ac 1µg, ab4729, Abcam). As wildtype FLI1 or ERG are not expressed in EWSR1-FLI1 or EWSR1-ERG positive EwS cell lines ...
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bioRxiv - Genetics 2021Quote: ChlPseq was performed on 20k FACS sorted MM cells as previously described 44 using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) but with minor modifications on the preparation of input controls ...
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... Sonification was performed for 10 sec at level 1 using “constant” mode with a 3 mm sonification tip (Branson) or in a Bioruptor Pico (Diagenode) for 2 cycles 30 sec each ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 min total (Diagenode).
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bioRxiv - Genomics 2022Quote: ... and 12 μg of high molecular weight gDNA was sheared to a size distribution between 15 and 20 kbp using the Megaruptor® 2 (Diagenode, Denville, NJ, USA). For each accession ...
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bioRxiv - Plant Biology 2021Quote: ... Nucleic acids were fragmented with a Diagenode Bioruptor® Sonicator (Diagenode, Belgium). For immunoprecipitation ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of antibody per IP (Cas9 – Diagenode C15310258 ...
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bioRxiv - Genomics 2024Quote: ... DNA was then sheared with Megaruptor 3 (Diagenode) (45/200/30 ...
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bioRxiv - Genomics 2020Quote: ... C15200211 (Diagenode, 5 μl/ChIP); H3K4me1 ...
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bioRxiv - Cancer Biology 2021Quote: ... sonicated 5 minutes (Bioruptor plus, Diagenode, 5 cycles high, 30 s on, 30 s off) and incubated at 37 °C for 60 min ...
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bioRxiv - Genomics 2020Quote: ... The lysates were sonicated with 5 × 5 min with 5s on/off intervals (Bioruptor, Diagenode) to get the DNA lengths between 200 and 1000 bp ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Genomics 2022Quote: ... and chromatin was fragmented into 1–5-kb fragments by sonication (high level, 33 cycles, 30 s ON, 50 s OFF) using a Bioruptor (Diagenode). Chromatin immunoprecipitation was used to enrich the complex fragments with magnetic beads of protein G (Thermo ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 µg of either IgG control antibody (Diagenode; C15410206) or of a specific antibody were applied ...