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Citations for Diagenode :
1 - 50 of 105 citations for 6 Heptynoic acid 2 amino 2S since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 50 ng/μl and sheared to 6 Kb fragments using Megaruptor® 2 (Diagenode, Seraing, Belgium). Shearing quality was estimated with TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: Two micrograms of genomic DNA were sonicated for 6 cycles (6 s on, 90 s off) at 4 °C with a Bioruptor NGS (Diagenode), generating average fragments of 1 kb ...
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bioRxiv - Genomics 2021Quote: ... while Lab 2 used Antibody 2 (Diagenode, Denville ...
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bioRxiv - Plant Biology 2021Quote: ... Nucleic acids were fragmented with a Diagenode Bioruptor® Sonicator (Diagenode, Belgium). For immunoprecipitation ...
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bioRxiv - Genomics 2023Quote: ... a Megaruptor 2 (Diagenode) was used to shear 3 μg of DNA to 15kb fragments ...
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bioRxiv - Cell Biology 2023Quote: ... H3K27me3 (Diagenode; #C15410195; 2 μg) and IgG-rabbit (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... H3K9me3 (Diagenode; #C15410193; 2 μg), H3K27me3 (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... for 6 cycles (1 min on/1 min off) on a Bioruptor Pico sonicator (Diagenode). For each ChIP reaction 4 % HEK293T-derived human spike-in lysate was combined with mESC lysate and incubated in 1x IP buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sonicated in 6 x 30 second intervals using a Bioruptor 300 Machine (Diagenode), then centrifuged for 10 minutes at 10,000g at 4°C ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... 2 μg anti-H3K27me3 (Diagenode, C15410195), and 2.5 μg anti-enhancer of zeste homolog 2 (EZH2 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 µg of 5mC antibody (Diagenode) were added to the sample and incubated for 2 h at 4°C with rotation ...
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bioRxiv - Biophysics 2024Quote: ... for 6 cycles (1 min on and 1 min off) on a Bioruptor Pico sonicator (Diagenode). ChIP lysates equivalent to 50 ug DNA were incubated in 1x IP buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-H4K20me3 (2 μl/ChIP, C15410207, Diagenode), anti-PA28γ (0.5 μl /ChIP ...
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bioRxiv - Cell Biology 2020Quote: ... anti-H3K9me3 (2 μl/ChIP, C15410056 Diagenode), anti-H3K4me3 (1 μl/ChIP ...
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bioRxiv - Microbiology 2021Quote: ... gDNA was sheared using Megaruptor 2 (Diagenode) to an average size of 8.9kb ...
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bioRxiv - Genomics 2021Quote: ... or 2 µg IgG control (Diagenode C15410296) was used ...
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bioRxiv - Developmental Biology 2019Quote: ... Homogenates were sonicated for 6 cycles of 30 seconds ON/30 seconds OFF on a PicoBioruptor (Diagenode), centrifuged for 10 minutes at 16,000 g at 4°C ...
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bioRxiv - Genomics 2021Quote: ... and sheared DNA was added to antibodies coupled to washed magnetic beads (anti-FLI1 2 µg, ab15289, Abcam; anti-ERG 2 µg, ab92513, Abcam; anti-H3K4me3 1.4 µg, C15410003, Diagenode; H3K27me3 2.9 µg ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 2% (v/v) 2-mercaptoethanol and samples were sonicated using a Bioruptor sonicator (Diagenode SA, Seraing, Belgium) at high amplitude for five 30 s on and off cycles ...
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bioRxiv - Genomics 2023Quote: ... and sonicated for 6 cycles of 30s ON/30s OFF on high power using the Bioruptor Plus (Diagenode). Insoluble material was removed by centrifugation at >16,000g for 10 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was resuspended in ice-cold lysis buffer (1xPBS, 2 mM PMSF, 2 mM DTT, 1 x protease inhibitors (Pierce)) and sonicated (Bioruptor Pico, Diagenode) at 4LJ with alternating 10 s burst/10 s break ...
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bioRxiv - Cell Biology 2020Quote: ... The dry samples were resuspended in 0.8 ml of 0.5% (v/v) trifluoroacetic acid (TFA) solution in HPLC-grade water with the assistance of sonication (Bioruptor Plus, Diagenode, 5 cycles of 30 s ON ...
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bioRxiv - Microbiology 2021Quote: ... but rather using the Megaruptor 2 system (Diagenode) with the software settings at 20Kb and following their user manual ...
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bioRxiv - Genetics 2022Quote: ... 0.5 μg (2 ul) of H3K9me3 antibody (Diagenode) and 0.5 μL of biotin-myc antibody (Abcam ...
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bioRxiv - Genomics 2023Quote: ... Size selection was performed with Megaruptor 2 (Diagenode) with default settings ...
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bioRxiv - Cell Biology 2019Quote: ... or Raji iBZLF1 cells induced for 6 h were lysed in RIPA buffer and sheared with the BioRuptor (Diagenode) four times (5 min ...
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bioRxiv - Genomics 2020Quote: ... genomic DNA was sheared using the Megaruptor 2 (Diagenode) with a long hydropore and a 20 kb shearing protocol ...
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bioRxiv - Plant Biology 2020Quote: ... samples were placed on ice for 10 min and sonicated for 6 cycles 15 sec “on” – 15 sec “off” using Bioruptor Plus (Diagenode). After that ...
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bioRxiv - Genomics 2022Quote: ... Cell lysis was facilitated by passing the lysates through 26G needle 10 times during the incubation on ice, and by 6 cycles of sonication (30 sec “on”/30 sec “off”, low input) on a Bioruptor Pico (Diagenode). Lysates were then treated with 40 U DNase I (Worthington ...
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bioRxiv - Microbiology 2023Quote: ... DNA was sonicated at 4°C (program: 6 cycles of 30 s on, 60 s off) on a Bioruptor Plus (Diagenode), achieving a size distribution between 200-1000 nucleotides (nt) ...
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bioRxiv - Molecular Biology 2023Quote: ... chromatin was sheared by sonication into 500 – 1000pb fragment (30sec ON/30sec OFF, 6 cycles, 4°C, Diagenode Bioruptor Pico). 10µl was kept aside as the input ...
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bioRxiv - Molecular Biology 2024Quote: ... and sonicated (6 cycles, high power for 30 s on and 30 s off at 4 °C, Bioruptor NextGen (Diagenode)) ...
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bioRxiv - Cell Biology 2021Quote: ... To shear any remaining nucleic acids, samples were sonicated in high mode (30 sec OFF, 30 sec ON) for 12 cycles (Bioruptor® Plus; Diagenode). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg DNA was sonicated in a Bioruptor Plus (Diagenode). DamID adaptors were removed by AlwI digestion ...
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bioRxiv - Developmental Biology 2019Quote: ... subtracted to 2 cycles of sonication on a bioruptor (Diagenode) with 30 sec “ON”/“OFF” at low setting and the remaining cell debris was removed by centrifugation at 21,000 g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... added (Figure 5B-5D) or 2x 300 µl was sonicated in 2x 1.5 ml bioruptor pico microtubes (Diagenode, Figure 6-9). When using the APLP protease inhibitors ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were first sonicated for 6 cycles of 30 sec on/30 sec off under low power setting with Bioruptor™ Twin (Diagenode), and then centrifuged at 17,000 × g for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... boiled for 5 min at 95°C 1000rpm in 2% SDC buffer (2% SDC, 100 mM Tris-HCl pH=8.5) and sonicated (Diagenode Bioruptor, 15 * 30s at high intensity). Protein concentration was determined via BCA Protein Assay (Thermo ...
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bioRxiv - Genomics 2023Quote: ... The samples were fragmented to 10 kb using Megaruptor 2 (Diagenode). For each fragmented sample ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of antibodies ChIP-grade (H3K4me2, Abcam ab32356; H3K4me3, Diagenode C15410003 ...
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bioRxiv - Cancer Biology 2021Quote: ... Then the solution was sonicated at 4 °C for 6 cycles (1cycle = 30 s sonication and 30 s cooldown with “High” energy intensity) using a Bioruptor® Plus sonication (Diagenode, UCD-300). After sonication ...
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bioRxiv - Immunology 2024Quote: ... We transferred cells into 1.5 ml sonication tubes (200 µL/tube) and sonicated 30 sec on/30 sec off for 6 cycles at 4 °C using Bioruptor® Pico (Diagenode, Denville, NJ). We centrifuged fragmented chromatin at 20,800 g for 10 min at 4 °C ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was sheared to 20 kb fragments using Megaruptor 2 (Diagenode) and size selected using the BluePippin system (Sage Science ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was sheared to 8 kb fragments using Megaruptor 2 (Diagenode). Standard SMRTbell libraries were prepared using Template Preparation Kit 1.0 (Pacific Biosciences ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... and 2.5 μg anti-enhancer of zeste homolog 2 (EZH2) (Diagenode, C15200180). Antibody-protein complexes were immunoprecipitated with protein A/G Dynabeads for 3 hours at 4°C with rotation ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were synthesized using a MicroPlex Library Preparation Kit (v.2; Diagenode). Libraries were sequenced as 2 × 50-bp reads on an Illumina HiSeq 2500 instrument ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 ug/ul of lysates were sonicated with a Bioruptor Plus (Diagenode) for 20 cycles (30 seconds ON and 30 seconds OFF) ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was fragmented to 20 kb using Megaruptor 2 (Diagenode, Liege, Belgium) and size selected with a 10 kb cut-off using the Blue Pippin system (Sage Science ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was sheared to 20 kb fragments using the Megaruptor 2 (Diagenode) and size selected with a 10 kb cut-off using the BluePippin system (Sage Science) ...
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bioRxiv - Genomics 2021Quote: ... Binding was stopped by adding 57 µL 2 M glycine (Diagenode, cat. C01011000) and incubating the sample for 5 min at room temperature ...