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Citations for Diagenode :
201 - 250 of 439 citations for 6 BROMO 4 4 DIETHYL 1H BENZO D 1 3 OXAZINE 2 4H THIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... rRNA depleted RPFs were directly used as input for the D-plex small RNA-seq kit (Diagenode cat#C05030001), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 25 ng of RNA in 8 µL water was input to the D-Plex Small RNA kit (Diagenode, C0503000) and libraries were prepared according to the manufacturer recommendations with the exception that 9 cycles of PCR were carried out ...
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bioRxiv - Plant Biology 2020Quote: ... samples were placed on ice for 10 min and sonicated for 6 cycles 15 sec “on” – 15 sec “off” using Bioruptor Plus (Diagenode). After that ...
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bioRxiv - Genomics 2022Quote: ... Cell lysis was facilitated by passing the lysates through 26G needle 10 times during the incubation on ice, and by 6 cycles of sonication (30 sec “on”/30 sec “off”, low input) on a Bioruptor Pico (Diagenode). Lysates were then treated with 40 U DNase I (Worthington ...
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bioRxiv - Genomics 2023Quote: DNA was sheared to an appropriate size range (10-20 kb) using Megaruptor 3 (Diagenode) followed by Ampure bead purification ...
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bioRxiv - Genomics 2023Quote: DNA was sheared to an appropriate size range (10-20 kb) using Megaruptor 3 (Diagenode) followed by SMRTbell cleanup beads ...
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bioRxiv - Neuroscience 2023Quote: ... and 8 μl was used for small RNA libraries construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). Indexes were attached using the D-Plex Single Indexes for Illumina - Set A (Diagenode C05030010 ...
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bioRxiv - Molecular Biology 2023Quote: Libraries were then prepared from 10 ng of RPFs using the D-Plex Small RNA-Seq kit for Illumina (Diagenode) and the D-Plex Unique Dual Indexes for Illumina – set A (Diagenode) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg DNA was sonicated in a Bioruptor Plus (Diagenode). DamID adaptors were removed by AlwI digestion ...
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bioRxiv - Developmental Biology 2019Quote: ... subtracted to 2 cycles of sonication on a bioruptor (Diagenode) with 30 sec “ON”/“OFF” at low setting and the remaining cell debris was removed by centrifugation at 21,000 g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... added (Figure 5B-5D) or 2x 300 µl was sonicated in 2x 1.5 ml bioruptor pico microtubes (Diagenode, Figure 6-9). When using the APLP protease inhibitors ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were first sonicated for 6 cycles of 30 sec on/30 sec off under low power setting with Bioruptor™ Twin (Diagenode), and then centrifuged at 17,000 × g for 10 min at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... 12 μg of DNA was prepared for sequencing using Megaruptor 3 shearing (Diagenode, settings 19/31) and the Express Template Prep Kit v2 and SMRTbell Cleanup Kit v2 (PacBio) ...
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bioRxiv - Neuroscience 2024Quote: ... The lysates were then sonicated with 3 × 10 cycles (30 s on and off) (Bioruptor, Diagenode) to generate chromatin fragments of ∼500 bp in length ...
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bioRxiv - Molecular Biology 2023Quote: ... boiled for 5 min at 95°C 1000rpm in 2% SDC buffer (2% SDC, 100 mM Tris-HCl pH=8.5) and sonicated (Diagenode Bioruptor, 15 * 30s at high intensity). Protein concentration was determined via BCA Protein Assay (Thermo ...
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bioRxiv - Neuroscience 2023Quote: ... and 20 ng of BH RNAs were used for small RNA library construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). D-Plex Single Indexes for Illumina - Set A (DiagenodeC05030010 ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed from 500 pg total RNA using D-Plex Small RNA-seq Kit for Illumina and Single Indexes for Illumina - Set B (Diagenode, C05030001). RIN aXer cell sorting was determined to be between 1.2 and 7.2 (Bioanalyzer 6000 ...
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bioRxiv - Genomics 2023Quote: ... The samples were fragmented to 10 kb using Megaruptor 2 (Diagenode). For each fragmented sample ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of antibodies ChIP-grade (H3K4me2, Abcam ab32356; H3K4me3, Diagenode C15410003 ...
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bioRxiv - Genomics 2020Quote: ... The latter aliquot was processed for ChIP using 3 μg anti-H3K4me3 antibody (pAb-003-050, Diagenode) as indicated in the user manual ...
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bioRxiv - Genetics 2020Quote: ... 30 s ON/30 s OFF for 15 min × 3 times in Bioruptor Sonication System UCD200 (Diagenode) in 15 ml polystyrene conical tubes ...
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bioRxiv - Genomics 2020Quote: ... the DNA was sheared to between 15 kb and 23 kb using the Megaruptor® 3 (Diagenode). HiFi sequencing libraries were prepared28 using SMRTbell™ Express Template Prep Kit 2.0 and followed by immediate treatment with the Enzyme Clean Up Kit (PN ...
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bioRxiv - Molecular Biology 2021Quote: ... then the sample was sonicated at a high intensity (3 × 5 min) using a Bioruptor sonicator (Diagenode). To obtain organoid-MPF cocultures ...
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bioRxiv - Cancer Biology 2023Quote: ... The pellets were resuspended in 300 μL Lysis Buffer 3 and sonicated by a Bioruptor Pico (Diagenode), resulting in chromatin fragments of around 300 to 500 bps ...
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bioRxiv - Genomics 2024Quote: ... HMW gDNA was sheared to 15-18 Kb using Diagenode’s Megaruptor 3 system (Diagenode, Belgium; cat. B06010003). The sheared gDNA was concentrated using 1x of SMRTbell cleanup beads for the repair and a-tailing incubation at 37°C for 30 minutes and 65°C for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Then the solution was sonicated at 4 °C for 6 cycles (1cycle = 30 s sonication and 30 s cooldown with “High” energy intensity) using a Bioruptor® Plus sonication (Diagenode, UCD-300). After sonication ...
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bioRxiv - Cancer Biology 2022Quote: ... Pellets were resuspended in 1.8mL of lysis buffer 3 (10mM Tris-HCl pH 7.5, 100mM NaCl, 1mM EDTA) and split in 6x 1.5mL sonication tubes (Diagenode). Upon 8 cycles of sonication with Pico Bioruptor (Diagenode ...
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bioRxiv - Cancer Biology 2021Quote: ... and sonicated (3 cycles of the 30 sec pulse with 30 sec interval on ice) using Bioruptor (Diagenode). The mixture was then boiled at 95 °C for 5 min to denature the proteins ...
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bioRxiv - Genomics 2023Quote: 10 ug of C5 genomic DNA was sheared to 30 kb - 50 kb using the Megaruptor 3 (Diagenode). The sheared DNA was treated with exonuclease to remove single-stranded ends ...
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bioRxiv - Genomics 2023Quote: ... and 10 µg of DNA were sheared to appropriate size range (15-20 kb) using Megaruptor 3 (Diagenode). The sheared DNA was concentrated by bead purification using PB Beads (PacBio) ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was sheared to 20 kb fragments using Megaruptor 2 (Diagenode) and size selected using the BluePippin system (Sage Science ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was sheared to 8 kb fragments using Megaruptor 2 (Diagenode). Standard SMRTbell libraries were prepared using Template Preparation Kit 1.0 (Pacific Biosciences ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... and 2.5 μg anti-enhancer of zeste homolog 2 (EZH2) (Diagenode, C15200180). Antibody-protein complexes were immunoprecipitated with protein A/G Dynabeads for 3 hours at 4°C with rotation ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were synthesized using a MicroPlex Library Preparation Kit (v.2; Diagenode). Libraries were sequenced as 2 × 50-bp reads on an Illumina HiSeq 2500 instrument ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 ug/ul of lysates were sonicated with a Bioruptor Plus (Diagenode) for 20 cycles (30 seconds ON and 30 seconds OFF) ...
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bioRxiv - Cancer Biology 2020Quote: ... chromatin was sheared to 100-500bp fragments using the Bioruptor Pico sonicator for 3 cycles of 30 sec on/off in 0.5ml tubes (Diagenode). Quantitation of chromatin was performed using a qubit fluorimeter where at least 10 ng of chromatin were used per experiment ...
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bioRxiv - Plant Biology 2022Quote: ... the protocol involves fragmenting the HMW DNA to a mean fragment length of 20 kb using Megaruptor 3 (Diagenode), followed by DNA damage repair ...
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bioRxiv - Molecular Biology 2019Quote: The soluble fraction was isolated by centrifugation at 2,000 rpm for 3 min then sonicated using a bioruptor (Diagenode) for 30 min in bursts of 30 s on/30 s off at high level in a 4°C water bath to produce sheared chromatin with a size range of 200-1,000 bp ...
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bioRxiv - Pathology 2022Quote: ... library was prepared using the EXP-NBD103 and SQK-LSK108 kits according to the manufacturer’s instructions and starting with 3 μg of 20 kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Microbiology 2022Quote: ... July 2020 release using the SMRTbell Express Template Prep Kit v2.0 with the following modifications: genomic DNA was sheared using Speed 34 on the Megaruptor 3 (Diagenode) and an additional size selection step of 6-50kb fragments on the Blue Pippin (Sage Science ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... by 30-min incubation on ice followed by sonicating for 3 min at medium power using a Bioruptor (Diagenode). Protein concentration was measured by Bradford assay (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: ... 3) around 400 μl of the slurry were transferred to 0.6 ml tubes and sonicated in the Bioruptor (Diagenode) at + 4°C for 1 hour on the “high” setting with sonication intervals set to 45 seconds on/15 seconds off ...
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bioRxiv - Neuroscience 2024Quote: ... the extracted DNA was sheared to a target size of 18-20 kb using the Megaruptor 3 (Diagenode, B060100003). After confirmation of correct sizing ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was fragmented to 20 kb using Megaruptor 2 (Diagenode, Liege, Belgium) and size selected with a 10 kb cut-off using the Blue Pippin system (Sage Science ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was sheared to 20 kb fragments using the Megaruptor 2 (Diagenode) and size selected with a 10 kb cut-off using the BluePippin system (Sage Science) ...
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bioRxiv - Genomics 2021Quote: ... Binding was stopped by adding 57 µL 2 M glycine (Diagenode, cat. C01011000) and incubating the sample for 5 min at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was sheared to 20-kb fragments using Megaruptor 2 (Diagenode, Belgium) and then bead-size selected with AMPure PB beads (Pacific Biosciences ...
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bioRxiv - Zoology 2022Quote: ... 2 μg genomic DNA was sonicated using a Picoruptor (Diagenode s.a., Liège, Belgium) to produce 300–500-bp fragments ...
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bioRxiv - Plant Biology 2022Quote: HMW DNA was sheared using Megaruptor 2 system (Diagenode, Liège Science Park, Belgium) to obtain a 40 Kb average size ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μl of precleared chromatin was added to 98 μl buffer iE1 (Diagenode). To samples and inputs ...