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Citations for Diagenode :
151 - 200 of 310 citations for 6 6 dimethyl 1 phenyl 6 7 dihydro 1H indazol 4 5H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Lysates were then subjected to five cycles (30 seconds on/30 seconds off) of sonication at 4°C on high intensity in a Bioruptor Sonication System (Diagenode) and subsequently cleared by centrifugation at 14,000g for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were sonicated using 40 cycles of 15s on/off at 4°C in the Bioruptor plus (model UCD-300, Diagenode). Further ...
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bioRxiv - Microbiology 2023Quote: ... was dissolved in 75 µL lysis buffer, boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). Lysates from the secretomes and intracellular fractions were centrifuged (10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... for 5 min at 4°C and lysates were sonicated (1x 5 min, 30 sec on/off) with a Bioruptor (Diagenode) followed by digestion with 40U RNaseI (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... sonicated for 10 cycles with 30 ‘’ on and 30 ‘’ off at maximum power at 4 °C using a Bioruptor Pico sonication device (Diagenode). Afterwards ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were sonicated at 4°C for 10 min (30 sec ON, 30 sec OFF) at medium frequency (Bioruptor Diagenode). Then ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was then de-crosslinked and eluted for 4 hours at 65C before being purified using IPure beads (Diagenode Cat# C03010014). The purified DNA was then subjected to sequencing library preparation or qPCR analysis ...
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bioRxiv - Plant Biology 2021Quote: ... The IP of the methylated DNA was performed overnight at 4°C using Protein A Dynabeads coated with anti-5mC (Diagenode, C15200081) or anti-IgG (Diagenode ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were incubated by rotating overnight at 4°C with primary antibody or irrelevant IgGs and then incubated with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 3 hr at 4 °C with rotation ...
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bioRxiv - Biochemistry 2022Quote: ... samples were sonicated at 4°C in 30 s on/off intervals for 15 cycles using a Bioruptor ® Plus sonication instrument (Diagenode) at high-intensity settings ...
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bioRxiv - Systems Biology 2021Quote: SARS-CoV-2 infected A549-ACE2 cell lysates were sonicated (10 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). Protein concentration was adjusted based on Pierce660 assay supplemented with ionic detergent compatibility reagent ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). HA or Streptactin beads were added to cleared lysates and samples were incubated for 3 hours at 4°C under constant rotation ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). Following normalization of protein concentrations of cleared lysates ...
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bioRxiv - Cell Biology 2021Quote: ... The homogenized samples were submitted to 10 cycles of 10 s at maximal intensity at 4⁰C in a sonicator bath (Bioruptor, Diagenode, USA). Then the homogenates were centrifuged at 10,000 g for 10 min at 4⁰C and the supernatants (NP40-soluble fraction ...
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bioRxiv - Genomics 2021Quote: ... pH 7.4) aided by homogenization by passing the cells through various gouge needles and sonication using the Bioruptor® sonication system (Diagenode) for 15 cycles of 30 s ON / 30 s OFF at high setting ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: Membranes disruption was carried by sonication at 4 °C (2x10 min, 30 sec on, 30 sec off, high setting, Diagenode Bioruptor). Extracts were cleared by centrifugation (15,000 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: Immunoprecipitation was performed using the IP star robot at 4°C (indirect 200 µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10 μg ...
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bioRxiv - Molecular Biology 2024Quote: ... for a total volume of 100 μL and sonicated (4 cycles of 20sec/30sec (on/off time)) using a Bioruptor (Pico II, Diagenode, BE). The successful generation of fragments with a mean fragment size of 400 nucleotides was confirmed using a Bioanalyzer as per manufacturer’s instructions (Agilent ...
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bioRxiv - Immunology 2022Quote: ... and was sonicated for 4 rounds of 15 cycles of 30 seconds on/30 seconds off on high power (Diagenode Biorupter plus). After sonication ...
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bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Neuroscience 2019Quote: ... Fig.2A) using a closed system ultrasonic disruptor (10 cycles of 30s ON/30s OFF, 4°C; Bioruptor Pico, Diagenode sa, Liege, Belgium). ChIP assays were then conducted using antisera to CTCF (61311 ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
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bioRxiv - Genomics 2021Quote: ... H3K27me3 (1:100, C15410069, Diagenode), and SOX9 (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... H3K9me3 (Diagenode; #C15410193; 1:1000) and H3K27me3 (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... H4K20me1 (Diagenode; #C15410034; 1:500), H3K9me3 (Diagenode ...
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bioRxiv - Developmental Biology 2023Quote: ... H3K4me3 (Diagenode #C15410003, 1 μg), H3K27me3 (Diagenode #C15410069 ...
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bioRxiv - Cell Biology 2023Quote: ... H3K4me3 (1:1000; Diagenode C15410003), H3S10ph clone MC463 (1:2000 ...
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bioRxiv - Genetics 2023Quote: ... H3K79me1 (1:1000; Diagenode, C15410082), H3K79me2 (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sox4 (1:4000, Diagenode #C15310129), GAPDH (1:4000 ...
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bioRxiv - Neuroscience 2024Quote: ... H3K27me3 (Diagenode, C1541095, 1:2000), Total H3 (Cell Signaling ...
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bioRxiv - Microbiology 2019Quote: ... incubated at 95°C for 10 min and further sonicated for 15 cycles à 30 s at high intensity level and 4°C using Bioruptor®Plus (Diagenode, Liege, Belgium). Protein concentrations were determined by measuring the tryptophan fluorescence at an emission wavelength of 350 nm using 295 nm for excitation with an Infinite® M1000 PRO microplate reader (Tecan ...
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bioRxiv - Cancer Biology 2023Quote: ... boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico, Diagenode; 4 cycles of 30s ON + 90s OFF). In both cases ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a maximum of 600 ng of DNA per accession was sheared at 4°C to a target average length of 400 bp using a Bioruptor® Pico sonication device (Diagenode, Denville, NJ, USA). Illumina libraries were prepared using a modified KAPA protocol with the KAPA LTP Library Preparation Kit (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... and H3K27me3 (Diagenode; #C15410195; 1:200). After washing ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-H3K36me3 (1:50, Diagenode C15200183), anti-H3K9me3 (1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-CTCF (1:400, Diagenode C15410210), anti-SMC1 (1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-H3K27ac (1:100, Diagenode C15410196), anti-H3K36me2 (1:50 ...
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bioRxiv - Plant Biology 2021Quote: ... anti-GT1 (1:75) and anti-YSPTSPS repeat S2Pho (RNA Pol II, B1 subunit; 1:200, Diagenode). The samples were washed for 8h at 4°C with gentle agitation with PBS (0.2% v/v Tween-20 ...
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bioRxiv - Neuroscience 2020Quote: ... H3K4me3 (Diagenode C15410003-50; rabbit; 1 µg), H3K27ac (Abcam ab177178 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Ty1 antibody (Diagenode, #C15200054, 1:3000) and Peroxidase AffiniPure donkey anti-rabbit IgG (Jackson ImmunoResearch 711-035-152 ...
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bioRxiv - Genomics 2020Quote: ... H3K27ac Antibody (C15410196, Diagenode, 1:600 ratio) were incubated with 40 µl of Dynabeads protein A/G (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... anti-H3K4me3 (1 μl/ChIP, C15410003 Diagenode) anti-H4K20me3 (2 μl/ChIP ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-H3K27me3 (Diagenode, dilution 1:1,000).
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bioRxiv - Cell Biology 2023Quote: ... 1:2000 C15200203 CRISPR/Cas9 7A9 (Diagenode), 1:1000 ab181602 GAPDH (Abcam)) ...
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bioRxiv - Cell Biology 2020Quote: ... H3K9me3 (Diagenode: Catalogue# pAb-056-050 1:1000) and H2AK119Ub (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... Rabbit anti-Cas9 antibody (Diagenode, C15310258, 1:1000), Mouse anti-β actin antibody (HRP conjugated ...
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bioRxiv - Genomics 2020Quote: ... rabbit polyclonal anti-H3K27me3 (1:1000; Diagenode C15410069); mouse monoclonal anti-p21 (1:500 ...