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Citations for Diagenode :
1 - 50 of 521 citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 2µg of antibody (Methyl cytosine: Diagenode MAb-006-100 ...
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bioRxiv - Cancer Biology 2021Quote: ... Adapter-ligated DNA was subjected to immunoprecipitation with a primary monoclonal antibody against 5-methyl cytosine (Diagenode C15200081) using a previously published protocol [71] ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Neuroscience 2021Quote: ... sonicated for 3×5 minutes in a 4°C Bioruptor ultrasonicator (UCD-200, Diagenode), vortexed ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequently disrupted by 2 × 20” high intensity sonication cycles at 4°C in a BioRuptor (Diagenode). Protein concentration was determined using the Micro BCA™ protein assay kit according to manufacturer’s instructions (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were sonicated (settings: 3 – 4 x 30 sec on /30 sec off, 4°C, power high; Bioruptor, Diagenode) and lysates were cleared by centrifugation at 16,000 x g at 4°C for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... and sonication bath (3×10 sec at 4°C) (Bioruptor, Diagenode). Lysates were centrifuged (14.000x g ...
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bioRxiv - Genomics 2023Quote: ... 7μL Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Cell Signalling Technology #9733) or 1μg negative control rabbit IgG (Diagenode) per ChIP reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... with following appropriate antibodies at 4 °C o/n a rotating wheel: H3K27me3 (Diagenode, C15410195), RING1B (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitation was done with 2 µg of chromatin and 4 µg of antibody against H3K9me3 (Diagenode, C15410193, lot A2217P). Sequencing libraries were prepared from 5 ng of immunoprecipitated DNA as previously described [19 ...
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bioRxiv - Genomics 2023Quote: Two micrograms of genomic DNA were sonicated for 6 cycles (6 s on, 90 s off) at 4 °C with a Bioruptor NGS (Diagenode), generating average fragments of 1 kb ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 95°C for 5 min and sonicated at 4°C for 2 cycles of 20 s at high intensity level using a BioRuptor (Diagenode). Samples were clarified by centrifugation at 16,000 x g for 15 min ...
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bioRxiv - Developmental Biology 2019Quote: ... and sonication for 2 cycles of 15s ON and 15s OFF on medium power at 4°C on a PicoBioruptor (Diagenode). After 10 minutes incubation at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... and sonicated for 2 cycles of 15s ON and 15s OFF on full power at 4°C on a Bioruptor (Diagenode). Samples were incubated for 1 hour on a rotating wheel at 4°C and centrifuged 10 minutes at 12,000 g and 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... which were incubated overnight on a rotatory wheel at 4°C with the primary antibodies H4K20me1 (Diagenode; #C15410034; 2 μg), H3K9me3 (Diagenode ...
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bioRxiv - Cell Biology 2020Quote: ... 4 × 5 min cycle (30 s on/ 30 s off; Diagenode Bioruptor) with 4 min incubation on ice between sonication cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Genomics 2021Quote: ... while Lab 2 used Antibody 2 (Diagenode, Denville ...
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bioRxiv - Plant Biology 2019Quote: ... For 5-mC immunolocalization slides were first incubated over night at 4°C with a monoclonal anti-5-mC-antibody (Diagenode C15200003 ...
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bioRxiv - Molecular Biology 2024Quote: ... for 5 min at 4°C and lysates were sonicated (1x 5 min, 30 sec on/off) with a Bioruptor (Diagenode) followed by digestion with 40U RNaseI (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... anti-Histone 4 rabbit (1:2,000; Diagenode Cat. No C15410156-50). HRP-conjugated anti-mouse and anti-rabbit antibodies were used (1:5,000 ...
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bioRxiv - Genomics 2021Quote: ... After sonication (15 sec on/ 30 sec off, 5 cycles, 4 C) using Bioruptor 300 (Diagenode) 5 µl were stained with DAPI to verify isolation of intact Macs ...
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bioRxiv - Molecular Biology 2022Quote: ... After sonication for 5 cycles of 30 seconds ON/OFF at 4 °C (Bioruptor Pico, Diagenode), the mixture was centrifuged (13 000xg ...
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bioRxiv - Microbiology 2023Quote: ... DNA was sonicated at 4°C (program: 6 cycles of 30 s on, 60 s off) on a Bioruptor Plus (Diagenode), achieving a size distribution between 200-1000 nucleotides (nt) ...
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bioRxiv - Molecular Biology 2023Quote: ... chromatin was sheared by sonication into 500 – 1000pb fragment (30sec ON/30sec OFF, 6 cycles, 4°C, Diagenode Bioruptor Pico). 10µl was kept aside as the input ...
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bioRxiv - Molecular Biology 2024Quote: ... and sonicated (6 cycles, high power for 30 s on and 30 s off at 4 °C, Bioruptor NextGen (Diagenode)) ...
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bioRxiv - Genomics 2022Quote: ... the nuclei were briefly sonicated for 5 seconds at 4°C in a Bioruptor Pico sonicator (Diagenode). Permeabilized nuclei were incubated for 10 minutes at 37°C in a thermoshaker ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2–5.5 µg of each sample was sheared using the Megaruptor 3 instrument (Diagenode, Liege, Belgium) at 18-20ng/µl and speed setting 31 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was resuspended in ice-cold lysis buffer (1xPBS, 2 mM PMSF, 2 mM DTT, 1 x protease inhibitors (Pierce)) and sonicated (Bioruptor Pico, Diagenode) at 4LJ with alternating 10 s burst/10 s break ...
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bioRxiv - Molecular Biology 2021Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Immunology 2021Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 × g ...
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bioRxiv - Immunology 2020Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 xg ...
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bioRxiv - Molecular Biology 2024Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Genomics 2023Quote: ... a Megaruptor 2 (Diagenode) was used to shear 3 μg of DNA to 15kb fragments ...
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bioRxiv - Microbiology 2023Quote: ... 40 mM 2-Chloroacetamide, 50 mM HEPES pH 8.5), boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). For the intracellular proteomes ...
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bioRxiv - Cell Biology 2020Quote: ... 600 ng of denatured DNA was incubated with 2 mg of 5-methylcytosine (5-mC) monoclonal antibody ([33D3] Diagenode, Cat No # C15200081) in IP buffer (0.1% SDS ...
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bioRxiv - Genetics 2023Quote: ... or 4 µg H3K9me3 antibody (C15410193, Diagenode) coupled to 30µL beads was added to 400µL soluble chromatin (Diagenode H3K9me3 antibody was used in Figure 2E only ...
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bioRxiv - Immunology 2024Quote: ... We transferred cells into 1.5 ml sonication tubes (200 µL/tube) and sonicated 30 sec on/30 sec off for 6 cycles at 4 °C using Bioruptor® Pico (Diagenode, Denville, NJ). We centrifuged fragmented chromatin at 20,800 g for 10 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split into 150 μl aliquots in 0.5 ml Eppendorf tubes and sonicated at 4°C for 30 cycles (30 seconds on/off) using a Bioruptor (Diagenode). Aliquots of the same samples were pooled and spun down at 12,000xg for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... were sonicated with 5 cycles of 30 s on followed by 30 s off at 4°C (Bioruptor Plus, Diagenode). After the centrifugation for 15 min at 4°C and 16,100 ×g ...
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bioRxiv - Microbiology 2023Quote: ... was dissolved in 75 µL lysis buffer, boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). Lysates from the secretomes and intracellular fractions were centrifuged (10 min ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 50 ng/μl and sheared to 6 Kb fragments using Megaruptor® 2 (Diagenode, Seraing, Belgium). Shearing quality was estimated with TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Genetics 2023Quote: ... Briefly, DNA from activated B cells (day 4 with IL-4, anti-CD40) was sonicated to ∼1000 bp (Diagenode Bioruptor) before amplification via an Sµ-specific biotinylated primer (5’/5BiosG/CAGACCTGGGAATGTATGGT-3’) ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Cell Biology 2023Quote: ... H3K27me3 (Diagenode; #C15410195; 2 μg) and IgG-rabbit (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... H3K9me3 (Diagenode; #C15410193; 2 μg), H3K27me3 (Diagenode ...
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bioRxiv - Microbiology 2020Quote: ... high power at 4°C (Bioruptor Plus, Diagenode)] ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). HA or Streptactin beads were added to cleared lysates and samples were incubated for 3 hours at 4°C under constant rotation ...