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Citations for Diagenode :
201 - 250 of 460 citations for 6 4 METHOXY PHENYL 2 PHENYL PYRAZOLO 1 5 A PYRIMIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed using 75 µg of chromatin and 5 µg of Cas9 antibody (Diagenode #C15200229-100) or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913 ...
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bioRxiv - Cancer Biology 2019Quote: ... and subsequently lysed by boiling for 5 min at 95°Cand sonication (Bioruptor, model ACD-200, Diagenode) for 15 min at level 5 (30 sec ON ...
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bioRxiv - Developmental Biology 2021Quote: 5×105 fixed nuclei were sonicated to a 200-500bp length with the Bioruptor Pico sonicator (Diagenode). H3K27Ac ChIP was (Diagenode C15410174 ...
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bioRxiv - Molecular Biology 2021Quote: ... then the sample was sonicated at a high intensity (3 × 5 min) using a Bioruptor sonicator (Diagenode). To obtain organoid-MPF cocultures ...
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bioRxiv - Plant Biology 2022Quote: ... Cells were ruptured by 5 × 15 sec sonication in an ice bath in Bioruptor UCD-200 (Diagenode) at medium intensity with 2 min breaks on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... and then sonicated for 5 min at high setting with 30sON/30sOFF in a Bioruptor sonicator (Diagenode) and centrifuged for 5 min at 19000g at 4°C ...
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bioRxiv - Genetics 2024Quote: ... 5 μg of genomic DNA from each treatment condition was sheared using a bioruptor sonication device (Diagenode) in low mode for two cycles (30s on + 60s off ...
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bioRxiv - Genetics 2021Quote: ... ONT libraries were prepared using the EXP-NBD103 and SQK-LSK109 kits according to the manufacturer’s instructions and using 4 µg of 40Kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Molecular Biology 2021Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Immunology 2021Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 × g ...
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bioRxiv - Genomics 2020Quote: ... For single crosslinked cells nuclei were sonicated for 4 cycles of 30s on/30s off using a Picoruptor (Diagenode). Immunoprecipitation was carried out overnight at 4°C using 2 μg of RUNX1 antibody (ab23980 ...
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bioRxiv - Immunology 2020Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 xg ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then incubated at 4°C overnight with or without 12.5 μg of anti-5hmC antibody (C15220001, Diagenode) in freshly prepared 1X Immunoprecipitation (IP ...
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bioRxiv - Immunology 2022Quote: ... Sonicated chromatin was diluted ten times in ChIP Dilution Buffer and immunoprecipitation was done overnight at 4°C by incubating 2.5 μl (7 μg) of anti-H3K27ac antibody (C15410196; Diagenode) precoated on 25-μl protein A-coated magnetic beads (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Chromatin was fragmented by sonication at 4°C using a Bioruptor 300 (Diagenode; 45 cycles, 30s on/30s off). Immunoprecipitation used 4 μg ChIP validated antibody against NF-κB p65 (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were sonicated for 5 cycles of 30 s on/off (2.5 min total sonication time) at 4°C in a Bioruptor Plus sonication system (Diagenode) and pelleted by centrifugation at 8000 RPM for 15 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with mixing for 30 min at 4°C before sonicating with a Bioruptor® Pico (Diagenode). Lysates were then spun for 10 min at 15,000 g ...
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bioRxiv - Plant Biology 2024Quote: ... Primary antibody incubation was performed overnight at 4°C with gentle shaking using anti-H3K9me2 (Diagenode Cat. No. C15410060), Anti-Histone H3 (Sigma-Aldrich Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Genomics 2019Quote: ... 10 mM EDTA (2 x 107 cell/ml) and sonicated in a Bioruptor device (Diagenode) for 25 min at 30 s intervals ...
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bioRxiv - Genetics 2020Quote: ... 2 cycles of sonication (30s ON/30s OFF) were performed using a Bioruptor Pico (Diagenode) to remove contaminants while minimising chromatin shearing74 ...
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bioRxiv - Genomics 2023Quote: ... The extracted DNA was sheared into 30 kb fragments using Megaruptor 2 (Diagenode, Seraing, Belgium), and then subjected to HiFi SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio) ...
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bioRxiv - Genetics 2023Quote: ... H3K79me3 (1:1000; Diagenode, C15410068, or 1:1000; Abcam, ab2621), H3K27me3 (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended nuclei were sonicated 5 times 30 sec on followed by 30 sec off using the Bioruptor (Diagenode). Insoluble proteins were removed by centrifugation for 10 min at 14,000 rpm at 4 °C.
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bioRxiv - Genomics 2019Quote: ... An aliquot of 5 µg DNA was mechanically sheared with a bath sonicator (Diagenode BioRuptor, Diagenode, Liège, Belgium) and low molecular weight fragments were used for the preparation of DNA libraries.
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bioRxiv - Genomics 2019Quote: ... An aliquot of 5 µg DNA was mechanically sheared with a bath sonicator (Diagenode BioRuptor, Diagenode, Liège, Belgium) and low molecular weight fragments were used for the preparation of DNA libraries.
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bioRxiv - Cancer Biology 2021Quote: ... Adapter-ligated DNA was subjected to immunoprecipitation with a primary monoclonal antibody against 5-methyl cytosine (Diagenode C15200081) using a previously published protocol [71] ...
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bioRxiv - Genomics 2023Quote: ... 5 × 105 fixed nuclei were sonicated to a 200–500 bp length with the Bioruptor Pico sonicator (Diagenode). H3K27Ac ChIP (Diagenode ...
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bioRxiv - Cancer Biology 2021Quote: ... lysed in 1% SDS containing buffer supplemented with 1x protease and phosphatase inhibitors, and sonicated for 4 x 10 cycles (30 sec on, 30 sec off) in temperature-controlled Bioruptor 300 (Diagenode). Debris was removed by centrifuging at 14,000 rpm at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... was then added at a final concentration of 1 % for 15 min at RT and samples were sonicated over 20 min (10 × 30 s pulses) at 4°C to cleave genomic DNA (Bioruptor, Diagenode). Anti-MAZ antibody was from Bethyl Laboratories (A301-652A) ...
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bioRxiv - Developmental Biology 2021Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitation was performed using IP star robot at 4°C (indirect 200µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10μg ...
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bioRxiv - Developmental Biology 2020Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were sonicated at 4° C for 20 minutes using a waterbath sonicator cycling 30 seconds on and 30 seconds off (Bioruptor Pico, Diagenode). Sonicated lysates were cleared by centrifugation at 18,000 g for 10 minutes at 4° C ...
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bioRxiv - Molecular Biology 2020Quote: ... with minor modifications: Cells were resuspended in PBS at 4°C after flow cytometry sorting and incubated with ChIP Crosslink Gold (Diagenode #C01019027 ...
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bioRxiv - Genomics 2021Quote: ... Lysates were sonicated for 15 cycles (30 sec on / 30 sec off) at 4°C using a Bioruptor Pico sonicator (Diagenode). After sonication ...
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bioRxiv - Cell Biology 2022Quote: ... cells were sonicated for 10 cycles of 30 sec “on” and 30 sec “off” at 4°C with Bioruptor (Diagenode) and the protein concentration was measured using Bradford protein assay (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were centrifuged at 14000rpm for 15mins at 4 oC and precleared using with 4ml of Magnetic A Beads (Diagenode) at 4 oC for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were sonicated for 4 min on a 30 s on/30 s off cycle using a Bioruptor Pico (Diagenode). Subsequent steps were performed as in the Universal Plant ChIP-seq kit protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were sonicated using 40 cycles of 15s on/off at 4 °C in the Bioruptor plus (model UCD-300, Diagenode) after boiling at 99 °C for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were extracted by sonication executing 40 cycles of 15s on and 15 s off at 4 °C using a Bioruptor plus (model UCD-300, Diagenode). After lysis ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell suspensions in 0.6 mL µtube were sonicated in water bath at 4°C during 12 min (15 sec. ON, 15 sec. OFF) with a Bioruptor apparatus (Diagenode) setted on high power and then clarified by 10 min centrifigation at 10 000 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... For protein extractions, the chromatin fraction was sonicated for 4 min (30s-on/30s-off, “high power”) on the Bioruptor Plus (Diagenode). Proteins’ content was quantified with the Thermo Fisher Scientific− BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Lysates were then subjected to five cycles (30 seconds on/30 seconds off) of sonication at 4°C on high intensity in a Bioruptor Sonication System (Diagenode) and subsequently cleared by centrifugation at 14,000g for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were sonicated using 40 cycles of 15s on/off at 4°C in the Bioruptor plus (model UCD-300, Diagenode). Further ...
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bioRxiv - Biochemistry 2023Quote: ... sonicated for 10 cycles with 30 ‘’ on and 30 ‘’ off at maximum power at 4 °C using a Bioruptor Pico sonication device (Diagenode). Afterwards ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were sonicated at 4°C for 10 min (30 sec ON, 30 sec OFF) at medium frequency (Bioruptor Diagenode). Then ...
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bioRxiv - Plant Biology 2019Quote: ... and sonicated 2 cycle x 30 second ON/ 30 second OFF using Bioruptor® Plus (Diagenode). After removal of cell debris by centrifugation (5 min ...
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bioRxiv - Microbiology 2023Quote: ... About 2 µg of genomic DNA was sheared by sonication with a Picoruptor (Diagenode, S.A., Belgium) instrument to a size of 300–400 bp in length ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2–5.5 µg of each sample was sheared using the Megaruptor 3 instrument (Diagenode, Liege, Belgium) at 18-20ng/µl and speed setting 31 ...