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Citations for Diagenode :
351 - 400 of 441 citations for 5 Methoxy 2 2 4 4 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... An aliquot of 5 µg DNA was mechanically sheared with a bath sonicator (Diagenode BioRuptor, Diagenode, Liège, Belgium) and low molecular weight fragments were used for the preparation of DNA libraries.
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bioRxiv - Genomics 2019Quote: ... An aliquot of 5 µg DNA was mechanically sheared with a bath sonicator (Diagenode BioRuptor, Diagenode, Liège, Belgium) and low molecular weight fragments were used for the preparation of DNA libraries.
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bioRxiv - Cancer Biology 2021Quote: ... Adapter-ligated DNA was subjected to immunoprecipitation with a primary monoclonal antibody against 5-methyl cytosine (Diagenode C15200081) using a previously published protocol [71] ...
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bioRxiv - Genomics 2023Quote: ... 5 × 105 fixed nuclei were sonicated to a 200–500 bp length with the Bioruptor Pico sonicator (Diagenode). H3K27Ac ChIP (Diagenode ...
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bioRxiv - Immunology 2022Quote: ... Cell lysate was transferred to 1.5 ml Bioruptor Plus TPX microtube (Diagenode) and was sonicated for 4 rounds of 15 cycles of 30 seconds on/30 seconds off on high power (Diagenode Biorupter plus) ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromatin was sheared by sonication in 15 ml Bioruptor tubes (Diagenode, C01020031) with 437.5 mg sonication beads (Diagenode ...
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bioRxiv - Developmental Biology 2022Quote: ... and the lysate was sonicated in 0.65 ml Bioruptor Pico Microtubes (Diagenode). Chromatin was sheared using a Bioruptor Pico (Diagenode ...
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bioRxiv - Genetics 2021Quote: Chromatin was transferred to a 15 ml conical tube (Diagenode, cat. # C30010017) for sonication in a Bioruptor Pico instrument (Diagenode ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin was sonicated in 1.5 mL TPX microtubes (#C30010010, Diagenode, Seraing, Belgium) for 20 cycles (30 sec on/30 sec off ...
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bioRxiv - Molecular Biology 2022Quote: ... transferred to 1.5 mL sonication tubes (Diagenode C30010016, 300 µL per tube), and sonicated for five min total (10 cycles of 30 s on/off ...
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bioRxiv - Biophysics 2024Quote: ... Chromatin was sheared by sonication in 15 ml Bioruptor tubes (Diagenode, C01020031) with 437.5 mg sonication beads (Diagenode ...
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bioRxiv - Neuroscience 2021Quote: ... the adaptor-ligated DNA was used for immunoprecipitation (IP) with a mouse monoclonal anti-5-hydroxymethylcytosine antibody (Diagenode, C15200200). For this ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (5 cycles, each 30 s on and 30 s off) using a Biorupter (Diagenode). A centrifugation step (at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended samples were boiled for 5 min at 95°C and sonicated for 15 min in the Bioruptor (Diagenode) at 30 sec on ...
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bioRxiv - Molecular Biology 2023Quote: ... High Molecular Weight Genomic DNA (5 µg) was sheared with the 40 kb program using a Diagenode Megaruptor (Diagenode) to generate DNA fragments of approximately 30 kb ...
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bioRxiv - Cancer Biology 2024Quote: ... Crosslinking was terminated by the addition of 1/10 volume 1.25 M glycine for 5 min at room temperature followed by cell lysis and sonication (Bioruptor, Diagenode), resulting in an average chromatin fragment size of 200 bp ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclear pellets were lysed with lysis buffer (50 mM Tris-HCl, 10 mM EDTA, 1% SDS, pH 8) and then sonicated with Bioruptor (Diagenode, 15 cycles, 30s ON/30s OFF). The size of the DNA in the sheared chromatin fragments was checked before precipitation by a Tapestation system (Agilent 4200 ...
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bioRxiv - Immunology 2020Quote: ... chromatin was sheared using a Bioruptor Pico Sonicator (5 cycles: 30 seconds ON - 30 seconds OFF, fragments 100 - 300 bp, Diagenode) and 10 % of sheared chromatin was kept as input control whilst 90 % was immunoprecipitated overnight using antibodies against H3K27ac ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation at 95C for 5 min the sample was sonicated 3 times for 30 sec on/off with a Bioruptor (Diagenode) on high power ...
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bioRxiv - Cell Biology 2020Quote: ... assisted by sonication (5 cycles of 30 s ON, 30 s OFF at high power, Bioruptor Plus ultrasonic disintegrator, Diagenode). Protein concentration was measured by the BCA assay.
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bioRxiv - Developmental Biology 2022Quote: ... in VFG media for 10 minutes at room temperature followed by a 5-minute quench with glycine (in True MicroChIP kit, Diagenode) at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Pathology 2021Quote: ... and fragmented by 5 cycles of sonication on a Bioruptor (per cycle: power = high, interval = 30s on/30s off, 5min; Diagenode) to facilitate efficient DNA clustering onto the Illumina sequencing platform ...
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bioRxiv - Microbiology 2019Quote: ... washed cell pellets were lysed in 50 mM HEPES pH 8 with 5% SDS followed by 10 min (30 sec on/off) sonication in a Bioruptor Pico sonicator (Diagenode) at 18 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... the remaining pellet was resuspended in 50μl RIPA supplemented with 1x Protease Inhibitor Cocktail and sonicated for 3-5 min with Picoruptor® (Diagenode). 10μg of proteins were then separated through precast 4-12% NuPAGE® Bis-Tris gels (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: MeDIP-Seq libraries for Ion Torrent semiconductor sequencing were performed on the Ion Torrent PGM using a modified protocol from Ion Plus Fragment Library kit (Catalog # 4471252) combined with a 5-hydroxymethylcytosine (5hmC Kit, Catalog # AF-110–0016) immunoprecipitation kit (Diagenode) according to a previously optimized protocol (Guerrero-Bosagna & Jensen ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2mM EDTA) and briefly sonicated to remove flagella (ON 5 sec – OFF 30 sec x 3 Cycles, bioruptor Pico, Diagenode). The samples were transferred in low retention tubes ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysis was obtained by pulsed ultrasonication (5 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300). Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to the supernatant and sonicated for 30 s ON/OFF for 5 min at high power in Bioruptor (Diagenode). After centrifugation at 16,000 × g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... beads were incubated for 30 min at RT with 5 µg of negative control antibody (C-, IgG from healthy rabbits, Diagenode). For immunoprecipitation ...
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bioRxiv - Bioengineering 2024Quote: ... The cell lysates were heated at 95[ for 5 minutes and sonicated for 15 min using a Bioruptor Plus (Diagenode). The supernatant was collected after centrifugation at 18,000 g for 30 min at 4[ ...
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bioRxiv - Cell Biology 2020Quote: ... The DNA was then transferred to 1.5 ml TPX tubes (Diagenode, C30010010-1000) and sonicated for 4 rounds of 10 minutes (high power ...
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bioRxiv - Genomics 2019Quote: ... The DNA was then transferred to 1.5 ml TPX tubes (Diagenode, C30010010-1000) and sonicated for 4 rounds of 10 minutes (high power ...
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bioRxiv - Genomics 2022Quote: ... shearing was performed in 0.65 ml Bioruptor® Pico Microtubes (Diagenode Cat# C30010011). 25μl of this chromatin was used to assess the size of the DNA fragments obtained by High Sensitivity NGS Fragment Analysis Kit (DNF-474 ...
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bioRxiv - Microbiology 2024Quote: ... by repeated slow-pipetting after transfer to 1.5 mL Bioruptor Micro tubes (Diagenode) on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... A portion of sheared DNA (10%) was kept as input and the rest of the sheared DNA was immunoprecipitated with α-5-methylcytosine antibody (Diagenode), bound to magnetic beads ...
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bioRxiv - Molecular Biology 2019Quote: ... and sonicated for 10 cycles with 30 s (on/off) intervals (5 min total sonication time) in a Diagenode Bioruptor Pico (Diagenode # B01060010). This nuclear lysate was cleared with centrifugation (13,500 g ...
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bioRxiv - Cancer Biology 2021Quote: ... sodium deoxycholate and 0.5% (vol/vol) N-lauroylsarcosine) and the chromatin was sheared to 300 – 800 base pair (bp) fragments by Bioruptor (Diagenode, Belgium) 12 cycles of 30 second “ON” and 30 second “OFF” intervals ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF, pH 8.5) and sonicated for 5 min (30 sec on, 1 min off) with Bioruptor UCD-200TM (Diagenode, Liège, Belgium). The resulting cell lysates were centrifuged ...
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bioRxiv - Genomics 2023Quote: ... A portion of sheared DNA (10 %) was kept as input and the rest of the sheared DNA was immunoprecipitated with α-5-methylcytosine antibody (Diagenode), bound to magnetic beads ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1% Tween-20) and lysed by pulsed ultrasonication (5 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300). Cell lysates were subjected to tandem affinity purification as described above ...
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bioRxiv - Genomics 2021Quote: ... 0.5 % NP-40) and evenly split into pre-cooled 1.5 ml Bioruptor tubes (Diagenode). After sonication (15 sec on/ 30 sec off ...
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bioRxiv - Immunology 2020Quote: 1.5 ml Bioruptor Microtubes were filled with 250 mg of Protein Extraction Beads (Diagenode) and filled with RIPA buffer (supplemented with protease and phosphatase inhibitors) ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei diluted in 1 ml RIPA buffer were sonicated using Bioruptor (Diagenode, Denville, NJ) for 6 cycles of 5 minutes (60 seconds on/30 seconds off ...
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bioRxiv - Neuroscience 2021Quote: ... 1mg/mL BSA) and sonicating at full strength for 15 min (Diagenode, Bioruptor Plus). Protein concentration was measured using a Bradford Assay and normalized so that all assay inputs contained the same amount of protein ...
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bioRxiv - Genetics 2022Quote: ... per 10 ml of solution and lysed by sonication in Bioruptor (UCD-200, Diagenode) with the following settings ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were transferred into 15-ml TPX tubes and sonicated using Bioruptor Plus (Diagenode) with the following parameters ...
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bioRxiv - Microbiology 2021Quote: ... boiled at 95°C for 10 min and sonicated for 5 min using a Bioruptor® Plus sonication device (Diagenode, Liège, Belgium). Protein lysates were then run on a precast NuPAGE Bis-Tris 4–12% SDS–PAGE (Thermo Fisher Scientific ...