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Citations for Diagenode :
351 - 400 of 426 citations for 6 Isoquinolinol 1 2 3 4 tetrahydro 1 4 methylphenyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was sheared to 20-kb fragments using Megaruptor 2 (Diagenode, Belgium) and then bead-size selected with AMPure PB beads (Pacific Biosciences ...
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bioRxiv - Zoology 2022Quote: ... 2 μg genomic DNA was sonicated using a Picoruptor (Diagenode s.a., Liège, Belgium) to produce 300–500-bp fragments ...
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bioRxiv - Plant Biology 2022Quote: HMW DNA was sheared using Megaruptor 2 system (Diagenode, Liège Science Park, Belgium) to obtain a 40 Kb average size ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μl of precleared chromatin was added to 98 μl buffer iE1 (Diagenode). To samples and inputs ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 × 107 cells / immunoprecipitation were fixed for 30 minutes (min) at room temperature (RT) using Diagenode Crosslink Gold (Diagenode, Liège, Belgium). After two washing steps with PBS (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA (1 μg) was fragmented to 300–600 bp after 25 cycles of 30 s of pulsed sonication in Bioruptor (Diagenode, Belgium). Fragmented DNA was end-repaired and adapter ligation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were kept on ice and processed directly or frozen at −80C in 20 ml of RNAse/DNAse free PBS supplemented with 1× protein inhibitor (Diagenode, Belgium). Embryos younger than late gastrula (younger than E7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF, pH 8.5) and sonicated for 5 min (30 sec on, 1 min off) with Bioruptor UCD-200TM (Diagenode, Liège, Belgium). The resulting cell lysates were centrifuged ...
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bioRxiv - Cell Biology 2023Quote: ... Each chromatin sample containing about 6,000 cells was incubated with 1 µg of anti-H3K27me3 antibody (Diagenode, C15410195, lot. A1811-001P). ChIP was performed using the automated platform SX-8G IP-Star platform (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... in 10 µl nuclease-free H2O and amplified with NEBNext® High-Fidelity 2X PCR Master Mix and 1 ul of primer pair from Diagenode (24 SI for tagmented libraries ...
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bioRxiv - Plant Biology 2023Quote: ... and cOmplete protease inhibitor cocktail tablets) and proceeded for sonication to shear the chromatin (∼ 500 bp to 1 kb size fragments) using Bioruptor Plus (Diagenode, USA) set at low mode and 45 cycles of 30 sec ON ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 30 min at 4°C with rocking and then sonicated (3 x 30 sec interval, high setting in a Bioruptor water bath sonicator, Diagenode). Lysates were cleared by centrifugation at 13,000 g for 15 min at 4°C ...
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bioRxiv - Genetics 2020Quote: ... The top 100 µl sperm suspension was sonicated at the medium level for five times with 3 seconds each (Bioruptor UCD-200; Diagenode). An aliquot of 2 µl sperm HTF suspension was mixed immediately with 50 µl of 4% PVP (Sigma ...
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bioRxiv - Genomics 2021Quote: ... we obtained 5 μg of high molecular weight genomic DNA (> 50% of fragments ≥ 40 kb) and sheared fragments to ~10 kb using the Megaruptor 3 (B06010003; Diagenode), followed by DNA repair and ligation of PacBio adapters using the SMRTbell Template Prep Kit v1.0 (100-991-900) ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation at 95C for 5 min the sample was sonicated 3 times for 30 sec on/off with a Bioruptor (Diagenode) on high power ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... in the presence of protease inhibitors and subjected to 3 × 10 cycles (30 seconds on and 30 seconds off) of sonication (Bioruptor, Diagenode) to generate chromatin fragments of ∼300 bp in length ...
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bioRxiv - Molecular Biology 2021Quote: ... the nuclear lysate was sonicated for 3 cycles of 30 seconds ON / 30 seconds OFF using a Bioruptor Pico (Diagenode) to ensure solubilisation of chromatin and then spun 18,000g for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... the remaining pellet was resuspended in 50μl RIPA supplemented with 1x Protease Inhibitor Cocktail and sonicated for 3-5 min with Picoruptor® (Diagenode). 10μg of proteins were then separated through precast 4-12% NuPAGE® Bis-Tris gels (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were sonicated briefly (3 cycles, 12 s on, 12 s off) at a high setting in a Bioruptor (Diagenode) sonicator ...
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bioRxiv - Genomics 2023Quote: ... Additional samples HG02554 and HG02953 were prepared for sequencing in the same way but with the following modifications: isolated DNA was sheared using the Megaruptor 3 instrument (Diagenode) twice using settings 31 and 32 to achieve a peak size of ∼15-20 kbp ...
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bioRxiv - Genomics 2023Quote: 15 μg of DNA was cleaned with a 1X AMPure PB beads (Pacific Biosciences) cleanup and sheared to a target size of 14 kilobases (kb) using the Megaruptor 3 instrument (Diagenode) with the following settings ...
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bioRxiv - Molecular Biology 2023Quote: ... 2mM EDTA) and briefly sonicated to remove flagella (ON 5 sec – OFF 30 sec x 3 Cycles, bioruptor Pico, Diagenode). The samples were transferred in low retention tubes ...
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bioRxiv - Genomics 2024Quote: HMW DNA was sheared to 20 kb using Covaris G-Tubes (for shallow-depth sequencing with MinION) or using the Megaruptor 3 (Diagenode; for high-depth sequencing with PromethION) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 mM PMSF) and chromatin was sheared by sonication to approximately 500-100 bp fragments using the Bioruptor® sonicator (Diagenode, Belgium). Immunoprecipitation was done with the anti-H3K27me3 (Active-motif ...
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bioRxiv - Genetics 2019Quote: ... 2 μl of IP material were resuspended in 30 ul ChIP-seq buffer (Diagenode) supplemented with 1 μl Tn5 (Illumina) ...
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bioRxiv - Neuroscience 2022Quote: ... Genomic DNA was sheared at 20kb target size with a Megaruptor 2 instrument (Diagenode). BluePippin size selection (Sage Science ...
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bioRxiv - Systems Biology 2023Quote: ... and 2 mM EDTA at pH 8.0) with H3K4me3 (0.005μg/μl, C15410003-50, Diagenode), H3K9me3 (0.005μg/μl ...
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bioRxiv - Genomics 2023Quote: ... 2 µg of genomic DNA were sheared on a Megaruptor3 instrument (Diagenode, Seraing, Belgium) to a fragment size of about 18 kb ...
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bioRxiv - Plant Biology 2022Quote: ... A HiFi library was then prepared according to the “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” manual with an initial DNA fragmentation by Megaruptor 3 (Diagenode, Belgium) and final library size binning into defined fractions by SageELF (Sage Science ...
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bioRxiv - Cancer Biology 2024Quote: ... buffer with added protease and phosphatase inhibitors and sonicated on high for 30 seconds off 30 seconds on for 3 minutes (Diagenode Bioruptor). The protein concentration was determined using the Micro BCA TM protein assay kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were incubated with blocking buffer (5% milk powder in TBST) for one hour at room temperature and afterwards incubated with primary antibody (anti-H3K27me3, Diagenode # C15410195, 1:1000) in blocking buffer overnight at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... libraries were constructed with 200 ng input DNA from each sample (n = 24; Table 1) using the Premium RRBS Kit (Diagenode, Cat. No. C02030032) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... PFFs were diluted in 1× PBS to a final concentration of 100 μL/Ml and sonicated with Bioruptor sonication device (Diagenode, Liege, Belgium, #B01020001) at the following settings ...
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bioRxiv - Genomics 2019Quote: ... 10 mM EDTA (2 x 107 cell/ml) and sonicated in a Bioruptor device (Diagenode) for 25 min at 30 s intervals ...
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bioRxiv - Genetics 2020Quote: ... 2 cycles of sonication (30s ON/30s OFF) were performed using a Bioruptor Pico (Diagenode) to remove contaminants while minimising chromatin shearing74 ...
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bioRxiv - Genomics 2023Quote: ... The extracted DNA was sheared into 30 kb fragments using Megaruptor 2 (Diagenode, Seraing, Belgium), and then subjected to HiFi SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio) ...
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bioRxiv - Developmental Biology 2021Quote: ... and subsequently sonicated for 3 cycles (30 seconds ON, 30 seconds OFF) on low settings using a Bioruptor® Plus (Diagenode, #B01020001). Samples were then centrifuged for 10 min at 10.000 g and supernatant was collected ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were crosslinked with 1% PFA at room temperature for 10 minutes and sonicated for 11 cycles (30 s ON/OFF, Diagenode Bioruptor Pico bath sonicator). 5% of the lysate was reserved as Input ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS and 0.5% N-lauroylsarcosine) and the chromatin was fragmented by sonication for 10 cycles on Bioruptor (Diagenode high settings, 30’’ on/off). Chromatin was separated from cell debris using centrifugation and stored at -80C for maximum of 2 months.
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bioRxiv - Plant Biology 2019Quote: ... and sonicated 2 cycle x 30 second ON/ 30 second OFF using Bioruptor® Plus (Diagenode). After removal of cell debris by centrifugation (5 min ...
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bioRxiv - Microbiology 2023Quote: ... About 2 µg of genomic DNA was sheared by sonication with a Picoruptor (Diagenode, S.A., Belgium) instrument to a size of 300–400 bp in length ...
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bioRxiv - Plant Biology 2023Quote: ChIP assays were performed on leaves and axillary buds enriched samples of 3 weeks old plants using anti-H3K27me3 (Diagenode pAb-195-050) and anti-IgG (Abcam ab6702) ...
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bioRxiv - Developmental Biology 2019Quote: ... the amplified cDNA (2 μg per sample) was fragmented to 200 bp using a Bioruptor Sonicator (Diagenode), and barcode ligation and end repair were performed using the Ovation Rapid DR Library System (NuGEN) ...
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bioRxiv - Genetics 2020Quote: ... The DNA was sonicated for 2 cycles of 15sec ON/90 sec OFF in a bioruptor (Diagenode) at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and the nuclei were resuspended in 50 µL transposition mixture (25 µL 2× Tagmentation buffer (Diagenode, C01019043), 2.5 µL Tagmentase loaded (Diagnode ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclear pellets were lysed with lysis buffer (50 mM Tris-HCl, 10 mM EDTA, 1% SDS, pH 8) and then sonicated with Bioruptor (Diagenode, 15 cycles, 30s ON/30s OFF). The size of the DNA in the sheared chromatin fragments was checked before precipitation by a Tapestation system (Agilent 4200 ...
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bioRxiv - Systems Biology 2020Quote: ... Chromatin was broken into small pieces with an average size of 0.5-2 kb using the Bioruptor (Diagenode). The sonicated chromatin was then incubated with 5 µg of primary antibodies overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2019Quote: HMW genomic DNA was sheared to 30 kb using a Megaruptor® 2 instrument (Diagenode SA, Seraing, Belgium). DNA-damage and end repair was carried out prior to blunt adaptor ligation and exonuclease purification using ExoIII and ExoVII ...