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Citations for Diagenode :
301 - 350 of 481 citations for SpectraDye Antibody Labeling Kit 550 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The sample was then incubated with antibodies (H3K27ac, Diagenode, C15410196; H3K27me3, Cell Signaling 9733S; H3K4me3, Diagenode C15410003 premium) coupled with protein A and protein G beads (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154; H3K27me1: Diagenode: C15410045-50) diluted in 1% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... the adaptor-ligated DNA was used for immunoprecipitation (IP) with a mouse monoclonal anti-5-hydroxymethylcytosine antibody (Diagenode, C15200200). For this ...
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bioRxiv - Genomics 2020Quote: ... then incubated with specific antibodies (anti-H3K27me3 Cell Signaling Technology 9733, anti-H3K36me2 CST 2901, anti-H3K27ac Diagenode C15410196), 1.5 million cells of sonicated cell lysate ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-cleared chromatin lysate was immunoprecipitated by a ChIP-grade rabbit polyclonal antibody against histone H3K9me3 (Cat. # C15410193, Diagenode) with end-over-end rotation (4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and DNA fragments (200 - 500 bp) were immunoprecipitated with a mouse monoclonal anti-5hmC antibody (2.5 μg per immunoprecipitation) following the protocol from Diagenode. The DNA-antibody mixture was incubated with magnetic beads overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then incubated at 4°C overnight with or without 12.5 μg of anti-5hmC antibody (C15220001, Diagenode) in freshly prepared 1X Immunoprecipitation (IP ...
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bioRxiv - Immunology 2022Quote: ... Sonicated chromatin was diluted ten times in ChIP Dilution Buffer and immunoprecipitation was done overnight at 4°C by incubating 2.5 μl (7 μg) of anti-H3K27ac antibody (C15410196; Diagenode) precoated on 25-μl protein A-coated magnetic beads (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Molecular Biology 2023Quote: ... and were directly subjected to immunoprecipitation with corresponding antibodies (H3K4me3: Diagenode, C15410003; H3K27me3: Diagenode, C15410195; CTCF: Millipore 07-792). Library preparation was carried out using NEBNext Ultra II DNA Library Prep kit (NEB E7645) ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated nuclei from primary fallopian tube secretory epithelial cells bound to concalavin A beads were incubated with primary antibody that detects H3K27ac (0.7μg, Diagenode, RRID:AB_2637079) at 4℃ overnight ...
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bioRxiv - Plant Biology 2024Quote: ... Primary antibody incubation was performed overnight at 4°C with gentle shaking using anti-H3K9me2 (Diagenode Cat. No. C15410060), Anti-Histone H3 (Sigma-Aldrich Cat ...
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bioRxiv - Genomics 2023Quote: ... The sample was then incubated with antibodies (AR: Abcam ab133273, FOXA1: Abcam ab23738, H3K27ac: Diagenode 3C15410196; H3K4me3: Diagenode 3C15410003) coupled with Dynabeads protein A and protein G beads (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitation was done with 2 µg of chromatin and 4 µg of antibody against H3K9me3 (Diagenode, C15410193, lot A2217P). Sequencing libraries were prepared from 5 ng of immunoprecipitated DNA as previously described [19 ...
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bioRxiv - Molecular Biology 2021Quote: ... The Low Cell# ChIP kit protein A × 48 was from Diagenode (Denville, NJ, USA) and the Chemiluminescence western blot detection reagent was from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2021Quote: Ribosome profiling libraries were prepared with the D-Plex Small RNA-Seq Kit (Diagenode). This method incorporates a 3′ end dephosphorylation step ...
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bioRxiv - Plant Biology 2020Quote: ... Library for Illumina sequencing was prepared using either MicroPlex Library Preparation™ Kit (Diagenode) according manufacturer instruction ...
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bioRxiv - Immunology 2020Quote: Chromatin immunoprecipitation was performed using the iDeal ChIP-seq kit for Transcription Factors (Diagenode), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: DNA sequencing libraries were prepared using the Diagenode MicroPlex Library Preparation kit (Diagenode # C05010014) according to the manufacturer’s instructions and sequenced on an Illumina NextSeq 500 platform with a 1×150 bp single-end read layout ...
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bioRxiv - Immunology 2021Quote: ... Chromatin immunoprecipitation was performed using the iDeal ChIP-seq kit for Transcription Factors (Diagenode) as per the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: ... RRBS libraries were prepared using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode) which uses the Mspl restriction enzyme and size selection to enrich for CpG-rich regions (coverage of about 4 million CpGs) ...
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bioRxiv - Immunology 2020Quote: ... The ChIP assay was performed using the iDeal ChIP kit for Transcription Factors (Diagenode) and sonication was performed using the Picoruptor™ (Diagenode ...
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bioRxiv - Microbiology 2022Quote: ... Chromatin was prepared for ChIPseq using the True MicroChIP Kit (Diagenode cat no. C01010130) and was sheared using Bioruptor® Pico sonication device (Diagenode cat no ...
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bioRxiv - Cancer Biology 2023Quote: The ChIP reaction was performed using the iDeal ChIP-seq kit (Diagenode, Cat# C01010055). Samples were pre-cleared using the beads provided in the kit and incubated overnight at 4°C with 5 µg of commercially available antibodies against lamin A/C (Abcam ...
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bioRxiv - Developmental Biology 2023Quote: For ChIP-seq the Diagenode iDeal ChIP-seq kit for Transcription Factors (Diagenode, C01010055) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... ChIP-seq libraries were made using the MicroPlex Library Preparation kit V2 (C05010012, Diagenode); and 50 bp single-end sequencing was performed at the Duke Sequencing and Analysis Core Resource on a Hi-Seq 2000 machine ...
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bioRxiv - Cancer Biology 2023Quote: ... from input and ChIP’d DNA using MicroPlex Library Preparation Kit v3 (Diagenode Cat# C05010001). Optimal library amplification was assessed by qPCR using KAPA SYBR® FAST (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... libraries for sequencing were prepared using the MicroPlex library preparation kit v2 (C05010014, Diagenode). Libraries were purified using AMPure XP purification beads (A63880 ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ChIPseq was performed as previously described (Bouderlique et al., 2019) using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) and the ThruPLEX DNA-seq (Rubicon Genomics ...
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bioRxiv - Evolutionary Biology 2019Quote: ... single male larvae were selected and processed following the low-input native chromatin immunoprecipitation protocol [72] with H3K9me3 and H4K16ac antibodies from Diagenode. The libraries were prepared using the SMARTer Universal Low Input DNA-seq Kit (Takara ...
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bioRxiv - Plant Biology 2019Quote: ... For 5-mC immunolocalization slides were first incubated over night at 4°C with a monoclonal anti-5-mC-antibody (Diagenode C15200003 ...
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was done on chromatin corresponding to the remaining 10 000 cells using antibodies against H3K4me3 (Diagenode, cat no. C15410003) – 10% of the chromatin was used for input ...
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bioRxiv - Genetics 2023Quote: ... We assessed two histone marks H3K4me3 and H3K27me3 and the ChIP grade antibodies against for these marks procured from Diagenode (H3K4me3 ...
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bioRxiv - Cell Biology 2023Quote: ... which were incubated overnight on a rotatory wheel at 4°C with the primary antibodies H4K20me1 (Diagenode; #C15410034; 2 μg), H3K9me3 (Diagenode ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed overnight at +4°C with primary antibodies (H3K4me2 1:1000; Abcam ab32356) (H3K4me3 1:200; Diagenode C15410003) (H3K4me2 1:1000 ...
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bioRxiv - Genomics 2023Quote: ... and the appropriate amount of antibody (1ug of α-H3K27ac and 0.5ug of α-H3K4me1; Diagenode #C15410196 and #C15410194 respectively). Samples were incubated rotating at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... beads were incubated for 30 min at RT with 5 µg of negative control antibody (C-, IgG from healthy rabbits, Diagenode). For immunoprecipitation ...
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bioRxiv - Biophysics 2024Quote: ... Click or tap here to enter text.Both strained culture plates and unstrained control plates were incubated with primary antibodies to detect H3K9me3 (Diagenode, C15200146) and HP1a (Abcam ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitated DNA was de-crosslinked and purified using the iPure Kit V2 (Diagenode, Cat#: C03010015) using the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... 73 µl of 1X buffer iC1 (from iDeal ChIP-seq kit for histones, Diagenode C01010173) supplemented with Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Immunology 2019Quote: ... Poly(A)+ RNA-seq libraries were constructed using CATS RNA-seq kit v2 (Diagenode, C05010041). Pooled libraries were sequenced on an Illumina HiSeq 2500 in rapid-run mode with paired-end reads.
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bioRxiv - Genomics 2021Quote: ... The RRBS libraries were prepared using the Diagenode Premium RRBS Kit (#C02030032, Diagenode, Denville, NJ) with the following exceptions ...
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bioRxiv - Neuroscience 2021Quote: ... The next steps were performed using iDeal ChIP-Seq kit for Transcription Factors (C01010055, Diagenode). Briefly ...
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bioRxiv - Genetics 2019Quote: ... using the iDeal ChIP-seq kit for Transcription Factors (catalogue number C01010055, Diagenode, Liege, Belgium). For each ChIP ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunoprecipitated DNA along with genomic DNA (Inputs) were purified using the IPure v2 kit (Diagenode). IP efficiency was confirmed by qPCR using primers provided in the kits ...
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bioRxiv - Plant Biology 2022Quote: ... Chromatin extraction and chromatin shearing were performed using a Universal Plant ChIP-seq kit (Diagenode) following the manufacturer’s instructions ...