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Citations for Diagenode :
251 - 300 of 341 citations for DNA Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... An aliquot of 5 µg DNA was mechanically sheared with a bath sonicator (Diagenode BioRuptor, Diagenode, Liège, Belgium) and low molecular weight fragments were used for the preparation of DNA libraries.
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bioRxiv - Genomics 2019Quote: ... An aliquot of 5 µg DNA was mechanically sheared with a bath sonicator (Diagenode BioRuptor, Diagenode, Liège, Belgium) and low molecular weight fragments were used for the preparation of DNA libraries.
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bioRxiv - Genomics 2021Quote: ... A total of 10 μg of DNA was purified then sheared at 40kb using the Megaruptor system (Diagenode). Using SMRTBell template Prep Kit 1.0 (PacBio) ...
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bioRxiv - Cancer Biology 2021Quote: ... Adapter-ligated DNA was subjected to immunoprecipitation with a primary monoclonal antibody against 5-methyl cytosine (Diagenode C15200081) using a previously published protocol [71] ...
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bioRxiv - Cancer Biology 2022Quote: ... sonicated until the lysates were clear of long DNA and other visible cell debris (Diagenode Bioruptor Plus sonicator). Lysates were processed by the Multi-Enzyme Digestion Filter Aided Sample Preparation (MED FASP ...
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bioRxiv - Developmental Biology 2021Quote: ... the synthetic DNA was fragmented into 350-bp pieces by a Bioruptor® Sonication System (UCD300, Diagenode Inc.), and the reactions were purified by incubation with Ampure XP beads (A63880 ...
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bioRxiv - Evolutionary Biology 2019Quote: HMW genomic DNA was sheared to 30 kb using a Megaruptor® 2 instrument (Diagenode SA, Seraing, Belgium). DNA-damage and end repair was carried out prior to blunt adaptor ligation and exonuclease purification using ExoIII and ExoVII ...
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bioRxiv - Genomics 2019Quote: ... Purified DNA was used as input for library preparation with MicroPlex Library Preparation Kit v2 (Diagenode, cat. C05010012) and processed according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2019Quote: ... Purified DNA was used as input for library preparation with MicroPlex Library Preparation Kit v2 (Diagenode, cat. C05010012) and processed according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... Phage DNA was sheared to fragments of approximately 350 bp using a Diagenode Bioruptor® Pico (Diagenode Diagnostics). One μg of DNA for each phage isolate was used for Illumina Truseq library preparation using the manufacturer’s PCR-free library preparation protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... To make sequencing libraries the PCR enriched genomic DNA sample was sonicated using a Bioruptor bath sonicator (Diagenode), purified using AMPure XP beads ...
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bioRxiv - Genomics 2023Quote: ... and 1 µg of DNA was purified and then sheared at 13 kb using the Megaruptor1 system (Diagenode). The library was size-selected ...
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bioRxiv - Genomics 2023Quote: 10 ug of C5 genomic DNA was sheared to 30 kb - 50 kb using the Megaruptor 3 (Diagenode). The sheared DNA was treated with exonuclease to remove single-stranded ends ...
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bioRxiv - Genomics 2023Quote: ... 15μg of DNA was purified then sheared at 15kb (speed 31 and 32) with the Megaruptor3 system (Diagenode). Using SMRTbell Express Template prep kit 2.0 ...
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bioRxiv - Plant Biology 2023Quote: ... approximately 200 ng of Arabidopsis genomic DNA was fragmented by sonication using a Bioruptor Pico (Diagenode, Denville, USA) for 6 cycles of 5 sec on and 30 sec off in 20 µl ddH2O ...
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bioRxiv - Genomics 2023Quote: ... and 10 µg of DNA were sheared to appropriate size range (15-20 kb) using Megaruptor 3 (Diagenode). The sheared DNA was concentrated by bead purification using PB Beads (PacBio) ...
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bioRxiv - Genomics 2024Quote: ... A total of 9.4μg of DNA was purified and then sheared at 20 kb using the Megaruptor system (Diagenode). Using SMRTbell® prep kit 3.0 ...
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bioRxiv - Genetics 2021Quote: ... ONT libraries were prepared using the EXP-NBD103 and SQK-LSK109 kits according to the manufacturer’s instructions and using 4 µg of 40Kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Neuroscience 2021Quote: ... the adaptor-ligated DNA was used for immunoprecipitation (IP) with a mouse monoclonal anti-5-hydroxymethylcytosine antibody (Diagenode, C15200200). For this ...
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bioRxiv - Molecular Biology 2022Quote: ... and chromosomal DNA sheared to 100 to 500 bp using Bioruptor Plus water bath sonication system (Diagenode, Liège, Belgium). After centrifugation to remove cell debris ...
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bioRxiv - Cell Biology 2022Quote: ... The amplified DNA (40 ng) was fragmented into ∼350 bp fragments using a Bioruptor® Sonication System (Diagenode Inc.). Illumina library construction was performed and qualified libraries were sequenced on the Illumina HiSeq platform ...
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bioRxiv - Genomics 2020Quote: Total genomic DNA was sheared by sonication for 15–24 min using a Bioruptor (Diagenode, Inc., New Jersey, USA). Size-selected samples (200–400 bp ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was fragmented with 2 rounds of 30 cycles 30sec ON-30sec OFF of sonication with Bioruptor Plus (Diagenode) set on HIGH and purified with Qiagen MinElute kit (Qiagen) ...
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bioRxiv - Plant Biology 2022Quote: ... the protocol involves fragmenting the HMW DNA to a mean fragment length of 20 kb using Megaruptor 3 (Diagenode), followed by DNA damage repair ...
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bioRxiv - Cancer Biology 2019Quote: ... immunoprecipitation and DNA purification was performed with reagents from iDeal ChIP-seq kit for Transcription Factors (Diagenode, ref: C01010054). Twenty cycles of sonication (30s high ...
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared from 10 ng of double-stranded purified DNA using the MicroPlex Library Preparation kit v2 (Diagenode) following the manufacturer’s protocol with some modifications ...
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bioRxiv - Genomics 2021Quote: ... Global DNA methylation profiling was generated with the Infinium MethylationEPIC BeadChip Kit (Queensland University of Technology and Diagenode, Austria). The first batch contained only males and were randomized for timepoint and age ...
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bioRxiv - Pathology 2022Quote: ... library was prepared using the EXP-NBD103 and SQK-LSK108 kits according to the manufacturer’s instructions and starting with 3 μg of 20 kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was sheared to a modal size of 10 kb using a Megaruptor 2 (Diagenode Diagnostics, Seraing (Ougrée), Belgium) ...
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bioRxiv - Microbiology 2022Quote: ... July 2020 release using the SMRTbell Express Template Prep Kit v2.0 with the following modifications: genomic DNA was sheared using Speed 34 on the Megaruptor 3 (Diagenode) and an additional size selection step of 6-50kb fragments on the Blue Pippin (Sage Science ...
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bioRxiv - Cancer Biology 2023Quote: ... ATAC-seq library preparation involved PCR amplification of the purified DNA fragments using Diagenode primer indices (Diagenode, Cat# C01011034). Amplification cycles were adjusted and libraries were purified using AMPure beads ...
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bioRxiv - Neuroscience 2024Quote: ... the extracted DNA was sheared to a target size of 18-20 kb using the Megaruptor 3 (Diagenode, B060100003). After confirmation of correct sizing ...
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bioRxiv - Molecular Biology 2023Quote: ... High Molecular Weight Genomic DNA (5 µg) was sheared with the 40 kb program using a Diagenode Megaruptor (Diagenode) to generate DNA fragments of approximately 30 kb ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The chromatin lysate was sonicated to a DNA fragment size range of 100-200bp using a Biorupter sonicator (Diagenode). CDK9 was immunoprecipitated with 15μg of an anti-CDK9 antibody (D-7 ...
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bioRxiv - Molecular Biology 2024Quote: ... was sheered using a Bioruptor® and the size of the sheered DNA was determined using a TapeStation 4200 according to the manufacturer’s instructions in the UTSA Genomics Core (Diagenode Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... was then added at a final concentration of 1 % for 15 min at RT and samples were sonicated over 20 min (10 × 30 s pulses) at 4°C to cleave genomic DNA (Bioruptor, Diagenode). Anti-MAZ antibody was from Bethyl Laboratories (A301-652A) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then sonicated until DNA was fragmented to an average distribution of about 200–300 bp using a Bioruptor (Diagenode) for a total of 30 cycles (30s on and 30s off) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total DNA was sheared to a mean target size of 350 bp with a Bioruptor® Pico sonication device (Diagenode). DNA were repaired ...
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bioRxiv - Genomics 2020Quote: DNA obtained from agarose plugs was sheared down to ~ 40 kbp fragment size with a MegaRuptor™ device (Diagenode, Belgium), fragmented using Covaris g-tubes (#520079 ...
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bioRxiv - Genetics 2020Quote: ... 50ng of immunoprecipitated DNA or input sample were used to prepare sequencing libraries using the iDeal Library Preparation kit (Diagenode), according to manufacturer instructions ...
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bioRxiv - Genomics 2019Quote: ... crosslinks were reversed and the free DNA was column purified and sonicated to approximately 600 bp peak fragment size (Bioruptor, Diagenode). Hi-C junctions were bound to streptavidin beads and washed to remove unbound DNA ...
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bioRxiv - Genomics 2021Quote: ... we obtained 5 μg of high molecular weight genomic DNA (> 50% of fragments ≥ 40 kb) and sheared fragments to ~10 kb using the Megaruptor 3 (B06010003; Diagenode), followed by DNA repair and ligation of PacBio adapters using the SMRTbell Template Prep Kit v1.0 (100-991-900) ...
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bioRxiv - Cancer Biology 2020Quote: ... 50mM Tris-Cl and protease inhibitor (Genetix)] followed by sonication for DNA fragmentation to an average fragment length of ∼500bp using Bioruptor (Diagenode). Sheared chromatin was incubated at 4°C overnight with 4μg of primary or isotype control antibodies ...
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bioRxiv - Genomics 2022Quote: ... genomic DNA was extracted and fragmented to a mean size of 250 bp by sonication using a Bioruptor (Diagenode, Belgium), followed by adding an “A” base to the 3’ terminal ends of DNA fragments and ligating methylated adaptors to both ends of DNA fragments ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells and nuclei were subsequently lysed and chromatin was sonicated to fragment DNA to about 200-500 bp on a Bioruptor Pico sonication device (Diagenode). Sonicated chromatin was pre-cleared with same-host IgG and protein G Dynabeads (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were resuspended in 1 mL of FA lysis buffer and subjected to DNA sonication for 20 cycles of 30 secs in a Bioruptor Twin (Diagenode). Following pelleting by 15 min centrifugation at 13000 rpm ...
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bioRxiv - Neuroscience 2019Quote: ... Libraries for reduced representation bisulfite sequencing (RRBS) were prepared from 100 ng DNA per sample using the Premium RRBS Kit (Diagenode). Final libraries were purified twice using Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Systems Biology 2020Quote: ... 140mM NaCl,0.1% Sodium deoxy cholate,10mM EDTA) and lysis and DNA fragmentation were carried out using a bioruptor (Diagenode, Nextgen) for 30 cycles (30 sec on and off cycles) ...
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bioRxiv - Molecular Biology 2019Quote: ... was added to ∼1ug yeast DNA and fragmented using a Bioruptor Pico instrument (Diagenode-13 cycles, 30s/30s On/Off). The DNA was end-repaired and ligated to methylated adapters ...
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bioRxiv - Microbiology 2020Quote: ... the whole genomic DNA was isolated and subjected to fragmentation by sonication using Diagenode Bioruptor Pico System (Diagenode Inc. USA). The fragmented DNA was run on 1% agarose gel and the 150 to 350 bp fragments were obtained and purified ...