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Citations for Diagenode :
251 - 300 of 492 citations for 2 Methoxy 2 3 4 5 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... sample DNA was ethanol precipitated and sheared in 500 µL sonication buffer (50 mM Tris pH 8.0, 0.1% SDS, 10 mM EDTA) on a Bioruptor Pico sonicator (Diagenode). DNA was then concentrated on Amicon ultra 0.5 30K filter units (Millipore) ...
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bioRxiv - Plant Biology 2020Quote: ... Beads were incubated for 10h with 50 µL of sheared chromatin diluted in ChIP dilution buffer (Diagenode, Belgium). One tenth of the diluted chromatin was collected and kept aside as an input sample before incubation with antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... and aliquoted into 1.5 mL microtubes (Diagenode). All of the above lysis buffers were supplemented with 250 μM Na3VO4 ...
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bioRxiv - Molecular Biology 2023Quote: ... in 15 ml sonication tubes (Diagenode, C01020031) using 350 mg/mL of sonication beads (Diagenode ...
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bioRxiv - Microbiology 2024Quote: ... in a 1.5 mL TPX microtube (Diagenode) and incubated on ice for 15 minutes ...
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bioRxiv - Genomics 2020Quote: ... The latter aliquot was processed for ChIP using 3 μg anti-H3K4me3 antibody (pAb-003-050, Diagenode) as indicated in the user manual ...
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bioRxiv - Genetics 2020Quote: ... 30 s ON/30 s OFF for 15 min × 3 times in Bioruptor Sonication System UCD200 (Diagenode) in 15 ml polystyrene conical tubes ...
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bioRxiv - Genomics 2020Quote: ... the DNA was sheared to between 15 kb and 23 kb using the Megaruptor® 3 (Diagenode). HiFi sequencing libraries were prepared28 using SMRTbell™ Express Template Prep Kit 2.0 and followed by immediate treatment with the Enzyme Clean Up Kit (PN ...
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bioRxiv - Cancer Biology 2023Quote: ... The pellets were resuspended in 300 μL Lysis Buffer 3 and sonicated by a Bioruptor Pico (Diagenode), resulting in chromatin fragments of around 300 to 500 bps ...
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bioRxiv - Genomics 2024Quote: ... HMW gDNA was sheared to 15-18 Kb using Diagenode’s Megaruptor 3 system (Diagenode, Belgium; cat. B06010003). The sheared gDNA was concentrated using 1x of SMRTbell cleanup beads for the repair and a-tailing incubation at 37°C for 30 minutes and 65°C for 5 minutes ...
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bioRxiv - Genomics 2020Quote: ... We sheared each BAC DNA (5 µg) separately using a Biorupter Pico (Diagenode) to 100-500 bp size ...
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bioRxiv - Cancer Biology 2021Quote: ... for sonication for 5 min (30 sec ON, 30 sec OFF) (Bioruptor Diagenode), yielding genomic DNA fragments with a bulk size of 150-600 bp ...
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bioRxiv - Neuroscience 2022Quote: ... before 5 cycles of 20 s on/off sonication using a Bioruptor (Diagenode). Samples were centrifuged for 15 min at 14,000 g to pellet insoluble debris and lipids ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... 30 µg of chromatin and 4 μl of mouse anti-ERalpha monoclonal antibody (C15100066, Diagenode) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... For each sample the Ab coating mix was done with 4 μl anti-H3K4me3 (Diagenode, Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... with following appropriate antibodies at 4 °C o/n a rotating wheel: H3K27me3 (Diagenode, C15410195), RING1B (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... and then sonicated for 4 minutes (30 sec on / off) using a Biorruptor Pico (Diagenode). Protein concentrations were determined by the BCA method (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 s on / 30 s off) at 4°C using a water bath-sonicator (Diagenode). Chromatin fragments were reverse cross-linked overnight at 65°C ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in 100 μL resuspension buffer (50 mM HEPES-NaOH pH 7.2, 150 mM KCl) and lysed for 10 minutes using the Bioruptor (Diagenode, 10 cycles ...
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bioRxiv - Microbiology 2019Quote: ... 50 mM Tris-HCl) by multiple pipetting and chromatin was fragmented to approximately nucleosome size using a BioruptorTM (Diagenode). After addition of 100 µl 10% Triton X-100 Cell debris was pelleted (20,000 x g ...
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bioRxiv - Cell Biology 2021Quote: ... and 50 mM Tris-HCl (pH 8.1)] in the presence of protease inhibitors and subjected to sonication (Bioruptor, Diagenode) to generate chromatin fragments of ~300 bp in length ...
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bioRxiv - Microbiology 2021Quote: Harvested cells were resuspended in a lysis buffer (50 mM Tris-HCl pH 8.0 and 150 mM NaCl) and were disrupted by a Bioruptor Pico device (Diagenode) through 12 cycles of sonication and intermittent rest at 10 sec and 45 sec ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were taken up in 100 μL LYSE buffer and mixed with 50 mg of protein extraction beads (Diagenode). Samples were sonicated with 10-20 cycles at 30sec ON/OFF in a Diagenode Bioruptor Plus until complete tissue disruption was observed ...
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bioRxiv - Cancer Biology 2022Quote: ... Pellets were resuspended in 1.8mL of lysis buffer 3 (10mM Tris-HCl pH 7.5, 100mM NaCl, 1mM EDTA) and split in 6x 1.5mL sonication tubes (Diagenode). Upon 8 cycles of sonication with Pico Bioruptor (Diagenode ...
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bioRxiv - Cancer Biology 2021Quote: ... and sonicated (3 cycles of the 30 sec pulse with 30 sec interval on ice) using Bioruptor (Diagenode). The mixture was then boiled at 95 °C for 5 min to denature the proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...
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bioRxiv - Genomics 2023Quote: ... and 10 µg of DNA were sheared to appropriate size range (15-20 kb) using Megaruptor 3 (Diagenode). The sheared DNA was concentrated by bead purification using PB Beads (PacBio) ...
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bioRxiv - Molecular Biology 2019Quote: ... changed to 1.5 ml Bioruptor Pico Microtubes (Diagenode) and sonicated in a Bioruptor Pico sonication device (Diagenode ...
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bioRxiv - Biophysics 2022Quote: ... Cells were lysed 1.5 mL Micro Tubes (Diagenode) by sonication using a Bioruptor® sonication system (Diagenode ...
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bioRxiv - Molecular Biology 2019Quote: ... or 5 μl H3K27me3 (Cell signaling 9733 or Diagenode C15410195 or Millipore 07-449). Beads were then washed quickly with RIPA buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... and then sonicated for 5 min at high intermittent pulses (30/30) (Diagenode, Bioruptor). Samples were centrifuged for 10 min at 10,000 g at 4°C to remove any cell debris and typically 25 μg proteins were separated on SDS-polyacrylamide gels and analysed by Western blotting ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed on ice for 5 minutes and sonicated by Bioruptor (Diagenode, B01020001) at the high setting for 5 minutes with 30 seconds intervals at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... and sonicated on ice for 5-10 min using a Bioruptor® sonicator (Diagenode). The cell lysate was centrifuged at high speed (16,000 g ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were sonicated (5x 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 5 µg of genomic DNA were sheared on a Megaruptor3 instrument (Diagenode, Seraing, Belgium) to a fragment size of about 13-16 kb ...
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bioRxiv - Molecular Biology 2021Quote: ... shear DNA and enhance cell disruption by using a water-bath (4°C) sonicator Bioruptor (Diagenode) at High Power for 40 min (sonication cycle ...
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bioRxiv - Genomics 2022Quote: ... Cell lysates were then sonicated 20 times at 4°C (Bioruptor Plus, Diagenode, 30/30 cycles) and centrifuged at 14000 rpm for 1 minute at 4°C to remove cellular debris ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated chromatin was fragmented by sonication at 4°C for 10 minutes using Bioruptor (Diagenode). Run-on reactions were performed at 37°C for 5 minutes using biotinylated nucleotides (5 mM Tris-HCl pH 8.0 ...
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bioRxiv - Physiology 2023Quote: ... 500µl volume) in a water bath sonicator set at 4°C (Bioruptor 300; Diagenode, Denville, NJ). After centrifuging at 10000g for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... A modified protocol for on-beads digest (sonication for 10*30s in a Bioruptor sonicator in presence of 50 mg Protein Extraction beads, C20000021, Diagenode) was used for tissue samples followed by a 3.5h digestion step ...
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bioRxiv - Cancer Biology 2023Quote: ... were lysed in buffer (50 mM Tris-HCl, pH 7.8) containing 1% SDS and 0.1 M dithiothreitol and sonicated (Diagenode Bioruptor Plus). Number of sonication cycles was established based on a visual sample clarity assessment ...
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bioRxiv - Genomics 2021Quote: ... 1 mL RIPA and 1 mL RIPA containing resuspended liver was placed into a 15 mL tube with 800 mg of Diagenode sonication beads (Diagenode, C01020031). This tube was sonicated for 30 cycles of 30s on and 30s off using a Bioruptor Pico ...
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bioRxiv - Cancer Biology 2020Quote: ... chromatin was sheared to 100-500bp fragments using the Bioruptor Pico sonicator for 3 cycles of 30 sec on/off in 0.5ml tubes (Diagenode). Quantitation of chromatin was performed using a qubit fluorimeter where at least 10 ng of chromatin were used per experiment ...
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bioRxiv - Plant Biology 2022Quote: ... the protocol involves fragmenting the HMW DNA to a mean fragment length of 20 kb using Megaruptor 3 (Diagenode), followed by DNA damage repair ...
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bioRxiv - Molecular Biology 2019Quote: The soluble fraction was isolated by centrifugation at 2,000 rpm for 3 min then sonicated using a bioruptor (Diagenode) for 30 min in bursts of 30 s on/30 s off at high level in a 4°C water bath to produce sheared chromatin with a size range of 200-1,000 bp ...
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bioRxiv - Pathology 2022Quote: ... library was prepared using the EXP-NBD103 and SQK-LSK108 kits according to the manufacturer’s instructions and starting with 3 μg of 20 kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Microbiology 2022Quote: ... July 2020 release using the SMRTbell Express Template Prep Kit v2.0 with the following modifications: genomic DNA was sheared using Speed 34 on the Megaruptor 3 (Diagenode) and an additional size selection step of 6-50kb fragments on the Blue Pippin (Sage Science ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... by 30-min incubation on ice followed by sonicating for 3 min at medium power using a Bioruptor (Diagenode). Protein concentration was measured by Bradford assay (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... the extracted DNA was sheared to a target size of 18-20 kb using the Megaruptor 3 (Diagenode, B060100003). After confirmation of correct sizing ...
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bioRxiv - Genomics 2020Quote: ... 5 μg of purified DNA was sheared at 8 kb using the megaruptor1 system (Diagenode), followed by a DNA damage repair step on 2 μg of sample ...