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Citations for Diagenode :
251 - 300 of 438 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... chromatin was sheared by sonication into 500 – 1000pb fragment (30sec ON/30sec OFF, 6 cycles, 4°C, Diagenode Bioruptor Pico). 10µl was kept aside as the input ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were centrifuged at 14000rpm for 15mins at 4 oC and precleared using with 4ml of Magnetic A Beads (Diagenode) at 4 oC for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were sonicated for 4 min on a 30 s on/30 s off cycle using a Bioruptor Pico (Diagenode). Subsequent steps were performed as in the Universal Plant ChIP-seq kit protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were sonicated using 40 cycles of 15s on/off at 4 °C in the Bioruptor plus (model UCD-300, Diagenode) after boiling at 99 °C for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were extracted by sonication executing 40 cycles of 15s on and 15 s off at 4 °C using a Bioruptor plus (model UCD-300, Diagenode). After lysis ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then subjected to five cycles (30 seconds on/30 seconds off) of sonication at 4°C on high intensity in a Bioruptor Sonication System (Diagenode) and subsequently cleared by centrifugation at 14,000g for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed overnight at +4°C with primary antibodies (H3K4me2 1:1000; Abcam ab32356) (H3K4me3 1:200; Diagenode C15410003) (H3K4me2 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... DNA was sonicated at 4°C (program: 6 cycles of 30 s on, 60 s off) on a Bioruptor Plus (Diagenode), achieving a size distribution between 200-1000 nucleotides (nt) ...
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bioRxiv - Neuroscience 2024Quote: ... on ice for 15 minutes and subjected to sonication for 4 separate cycles of 30s on and 30s off cycles at high setting (Bioruptor Plus, Diagenode). The resultant chromatin was centrifuged at 12000 rpm for 30 minutes to remove any debris ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were incubated overnight at 4°C with rotation in wash buffer with rabbit anti-Chd1 (Diagenode 15410334, 1:50). Nuclei were incubated for 1 hour at room temperature with rotation with rabbit anti-H3K4me3 (Sigma-Aldrich 05-745R ...
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bioRxiv - Cancer Biology 2024Quote: ... The suspension was sonicated for 15 cycles (30 seconds on/ 30 seconds off) at 4 ◦C (Diagenode Pico Bioruptor sonicator). Chromatin concentration was quantified using the Qubit 4 Fluorometer and Qubit dsDNA Broad Range kit ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT) and sonicated for 3x 10 seconds using a Bioruptor Pico (Diagenode). Cells were then rotated at 4°C for 20 mins before the lysates were diluted with 500 µl of no salt buffer (50 mM Tris-HCl ...
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bioRxiv - Genomics 2020Quote: ... Chromatin was sheared 10 × 30 sec on/30 sec off with a Bioruptor Pico (Diagenode). Supernatant with chromatin extract was isolated after a 10 minutes centrifugation at 14.000g at 4°C ...
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bioRxiv - Physiology 2021Quote: ... 10% glycerol) supplemented with protease and phosphatase inhibitor cocktails and sonicated in a Bioruptor (Diagenode). Protein lysates were quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: DNA was sheared to an appropriate size range (10-20 kb) using Megaruptor 3 (Diagenode) followed by SMRTbell cleanup beads ...
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bioRxiv - Genomics 2023Quote: DNA was sheared to an appropriate size range (10-20 kb) using Megaruptor 3 (Diagenode) followed by Ampure bead purification ...
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bioRxiv - Genomics 2022Quote: ... and fragmented by a Bioruptor Pico (10 cycles, 30 sec on/30 sec off; Diagenode). The sonicated solution was diluted (1:5 ratio ...
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bioRxiv - Plant Biology 2024Quote: ... Sheared chromatin (10 cycles, 30 s ON/ 30 s OFF using a Bioruptor® (Diagenode)) extracted from 100 mg material was equally divided between input and samples immunoprecipitated with anti-histone H3 (Merc ...
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bioRxiv - Immunology 2024Quote: ... Samples were boiled for 10 min at 95 °C and sonicated in a Bioruptor (Diagenode) for 10 cycles of 30 sec on/30 sec off ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysis and nuclei isolation were carried out in 10 mL of LB1 buffer (Diagenode) by rotating 20 min at 4 C ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was then de-crosslinked and eluted for 4 hours at 65C before being purified using IPure beads (Diagenode Cat# C03010014). The purified DNA was then subjected to sequencing library preparation or qPCR analysis ...
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bioRxiv - Plant Biology 2021Quote: ... The IP of the methylated DNA was performed overnight at 4°C using Protein A Dynabeads coated with anti-5mC (Diagenode, C15200081) or anti-IgG (Diagenode ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were incubated by rotating overnight at 4°C with primary antibody or irrelevant IgGs and then incubated with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 3 hr at 4 °C with rotation ...
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bioRxiv - Biochemistry 2022Quote: ... samples were sonicated at 4°C in 30 s on/off intervals for 15 cycles using a Bioruptor ® Plus sonication instrument (Diagenode) at high-intensity settings ...
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bioRxiv - Genomics 2021Quote: ... pH 7.4) aided by homogenization by passing the cells through various gouge needles and sonication using the Bioruptor® sonication system (Diagenode) for 15 cycles of 30 s ON / 30 s OFF at high setting ...
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bioRxiv - Molecular Biology 2024Quote: ... for a total volume of 100 μL and sonicated (4 cycles of 20sec/30sec (on/off time)) using a Bioruptor (Pico II, Diagenode, BE). The successful generation of fragments with a mean fragment size of 400 nucleotides was confirmed using a Bioanalyzer as per manufacturer’s instructions (Agilent ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Biochemistry 2023Quote: Immunoprecipitation was performed using the IP star robot at 4°C (indirect 200 µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10 μg ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: Membranes disruption was carried by sonication at 4 °C (2x10 min, 30 sec on, 30 sec off, high setting, Diagenode Bioruptor). Extracts were cleared by centrifugation (15,000 rpm ...
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bioRxiv - Neuroscience 2021Quote: ... the adaptor-ligated DNA was used for immunoprecipitation (IP) with a mouse monoclonal anti-5-hydroxymethylcytosine antibody (Diagenode, C15200200). For this ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (5 cycles, each 30 s on and 30 s off) using a Biorupter (Diagenode). A centrifugation step (at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended samples were boiled for 5 min at 95°C and sonicated for 15 min in the Bioruptor (Diagenode) at 30 sec on ...
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bioRxiv - Molecular Biology 2023Quote: ... High Molecular Weight Genomic DNA (5 µg) was sheared with the 40 kb program using a Diagenode Megaruptor (Diagenode) to generate DNA fragments of approximately 30 kb ...
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Spirolactone, an unprecedented antifungal β-lactone spiroketal macrolide from Streptomyces iranensisbioRxiv - Biochemistry 2024Quote: ... sonicating on high for 5 x 60 sec on/30sec off using the bioruptor pico sonication water bath (Diagenode) and finally disrupting twice with the tissuelyser (QIAGEN ...
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bioRxiv - Cell Biology 2024Quote: ... After 2 × 5 cycles of sonication (30 s “ON”, 30 s “OFF”, High intensity) with a Bioruptor UCD200 (Diagenode), the chromatin solution was diluted 10-fold to a final volume of 3 mL with ChIP dilution buffer before IP ...
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bioRxiv - Immunology 2022Quote: ... and was sonicated for 4 rounds of 15 cycles of 30 seconds on/30 seconds off on high power (Diagenode Biorupter plus). After sonication ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Slides were blocked with 4% BSA for 30 minutes at 37°C in a moist box followed by primary (anti-H3K9m2, C15410060, Diagenode, 1:500) and secondary (Alexa Fluor 488 Goat anti-Rabbit IgG1 Secondary Antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
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bioRxiv - Genomics 2024Quote: ... at 4°C for 13 cycles with 30 sec on and 30 sec rest in 15 ml Bioruptor Tubes (Diagenode Cat #C30010017) with 650 mg of beads (Diagenode Cat #C03070001) ...
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bioRxiv - Neuroscience 2024Quote: ... and sonicated for 15 min (cycles of 30 sec ON and 30 sec OFF, 4°C, low frequency) in a Bioruptor Pico (Diagenode, Seraing, Belgium). Samples were subsequently incubated for 15 min at 70°C ...
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bioRxiv - Immunology 2024Quote: ... by sonication for 15 min (30 s on/off cycles) at 4 °C with high power in a Bioruptor device (Diagenode, Liège, Belgium). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... chromatin was sheared using a Bioruptor Pico Sonicator (5 cycles: 30 seconds ON - 30 seconds OFF, fragments 100 - 300 bp, Diagenode) and 10 % of sheared chromatin was kept as input control whilst 90 % was immunoprecipitated overnight using antibodies against H3K27ac ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation at 95C for 5 min the sample was sonicated 3 times for 30 sec on/off with a Bioruptor (Diagenode) on high power ...
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bioRxiv - Cell Biology 2020Quote: ... assisted by sonication (5 cycles of 30 s ON, 30 s OFF at high power, Bioruptor Plus ultrasonic disintegrator, Diagenode). Protein concentration was measured by the BCA assay.
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bioRxiv - Genomics 2022Quote: ... and chromatin was fragmented into 1–5-kb fragments by sonication (high level, 33 cycles, 30 s ON, 50 s OFF) using a Bioruptor (Diagenode). Chromatin immunoprecipitation was used to enrich the complex fragments with magnetic beads of protein G (Thermo ...
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bioRxiv - Pathology 2021Quote: ... and fragmented by 5 cycles of sonication on a Bioruptor (per cycle: power = high, interval = 30s on/30s off, 5min; Diagenode) to facilitate efficient DNA clustering onto the Illumina sequencing platform ...
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bioRxiv - Cancer Biology 2021Quote: ... the remaining pellet was resuspended in 50μl RIPA supplemented with 1x Protease Inhibitor Cocktail and sonicated for 3-5 min with Picoruptor® (Diagenode). 10μg of proteins were then separated through precast 4-12% NuPAGE® Bis-Tris gels (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to the supernatant and sonicated for 30 s ON/OFF for 5 min at high power in Bioruptor (Diagenode). After centrifugation at 16,000 × g for 10 min at 4 °C ...