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Citations for Diagenode :
201 - 250 of 521 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the extracted DNA was sheared to a target size of 18-20 kb using the Megaruptor 3 (Diagenode, B060100003). After confirmation of correct sizing ...
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bioRxiv - Cell Biology 2020Quote: ... 600 ng of denatured DNA was incubated with 2 mg of 5-methylcytosine (5-mC) monoclonal antibody ([33D3] Diagenode, Cat No # C15200081) in IP buffer (0.1% SDS ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... The eluates were diluted 1:50 with ChIP buffer from the One Day ChIP kit (Diagenode) and subsequently subjected to a second ChIP in accordance with the One Day ChIP kit manual but performing an overnight antibody incubation under rotation at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 30 min at 4°C with rocking and then sonicated (3 x 30 sec interval, high setting in a Bioruptor water bath sonicator, Diagenode). Lysates were cleared by centrifugation at 13,000 g for 15 min at 4°C ...
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bioRxiv - Genetics 2020Quote: ... The top 100 µl sperm suspension was sonicated at the medium level for five times with 3 seconds each (Bioruptor UCD-200; Diagenode). An aliquot of 2 µl sperm HTF suspension was mixed immediately with 50 µl of 4% PVP (Sigma ...
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bioRxiv - Genomics 2021Quote: ... we obtained 5 μg of high molecular weight genomic DNA (> 50% of fragments ≥ 40 kb) and sheared fragments to ~10 kb using the Megaruptor 3 (B06010003; Diagenode), followed by DNA repair and ligation of PacBio adapters using the SMRTbell Template Prep Kit v1.0 (100-991-900) ...
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bioRxiv - Cell Biology 2019Quote: ... in the presence of protease inhibitors and subjected to 3 × 10 cycles (30 seconds on and 30 seconds off) of sonication (Bioruptor, Diagenode) to generate chromatin fragments of ∼300 bp in length ...
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bioRxiv - Molecular Biology 2021Quote: ... the nuclear lysate was sonicated for 3 cycles of 30 seconds ON / 30 seconds OFF using a Bioruptor Pico (Diagenode) to ensure solubilisation of chromatin and then spun 18,000g for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were sonicated briefly (3 cycles, 12 s on, 12 s off) at a high setting in a Bioruptor (Diagenode) sonicator ...
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bioRxiv - Genomics 2023Quote: ... Additional samples HG02554 and HG02953 were prepared for sequencing in the same way but with the following modifications: isolated DNA was sheared using the Megaruptor 3 instrument (Diagenode) twice using settings 31 and 32 to achieve a peak size of ∼15-20 kbp ...
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bioRxiv - Genomics 2023Quote: 15 μg of DNA was cleaned with a 1X AMPure PB beads (Pacific Biosciences) cleanup and sheared to a target size of 14 kilobases (kb) using the Megaruptor 3 instrument (Diagenode) with the following settings ...
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bioRxiv - Genomics 2024Quote: HMW DNA was sheared to 20 kb using Covaris G-Tubes (for shallow-depth sequencing with MinION) or using the Megaruptor 3 (Diagenode; for high-depth sequencing with PromethION) ...
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bioRxiv - Plant Biology 2022Quote: ... A HiFi library was then prepared according to the “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” manual with an initial DNA fragmentation by Megaruptor 3 (Diagenode, Belgium) and final library size binning into defined fractions by SageELF (Sage Science ...
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bioRxiv - Cancer Biology 2024Quote: ... buffer with added protease and phosphatase inhibitors and sonicated on high for 30 seconds off 30 seconds on for 3 minutes (Diagenode Bioruptor). The protein concentration was determined using the Micro BCA TM protein assay kit (Thermo Fisher) ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 50 ng/μl and sheared to 6 Kb fragments using Megaruptor® 2 (Diagenode, Seraing, Belgium). Shearing quality was estimated with TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... and subsequently sonicated for 3 cycles (30 seconds ON, 30 seconds OFF) on low settings using a Bioruptor® Plus (Diagenode, #B01020001). Samples were then centrifuged for 10 min at 10.000 g and supernatant was collected ...
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bioRxiv - Plant Biology 2023Quote: ChIP assays were performed on leaves and axillary buds enriched samples of 3 weeks old plants using anti-H3K27me3 (Diagenode pAb-195-050) and anti-IgG (Abcam ab6702) ...
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bioRxiv - Genomics 2023Quote: ... 7μL Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Cell Signalling Technology #9733) or 1μg negative control rabbit IgG (Diagenode) per ChIP reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 % Triton X-100) supplemented with 1 x HALT in a 15 ml Bioruptor tube containing one scoop of sonication beads (Diagenode) and sonicated in Bioruptor Pico (Diagenode ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... For indicated experiments the One Day ChIP kit (Diagenode) was used according to the protocol of the manufacturer.
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was resuspended in ice-cold lysis buffer (1xPBS, 2 mM PMSF, 2 mM DTT, 1 x protease inhibitors (Pierce)) and sonicated (Bioruptor Pico, Diagenode) at 4LJ with alternating 10 s burst/10 s break ...
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bioRxiv - Cancer Biology 2019Quote: ... one for IgG control and other for H3K27 acetylated antibody from Diagenode. Downstream processing of the chromatin bound antibody was done as per the manufacturer’s protocol for EZ-magna ChIP A/G (Millipore ...
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bioRxiv - Genomics 2021Quote: ... while Lab 2 used Antibody 2 (Diagenode, Denville ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 min total (Diagenode).
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bioRxiv - Genomics 2020Quote: ... C15200211 (Diagenode, 5 μl/ChIP); H3K4me1 ...
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bioRxiv - Cancer Biology 2021Quote: ... sonicated 5 minutes (Bioruptor plus, Diagenode, 5 cycles high, 30 s on, 30 s off) and incubated at 37 °C for 60 min ...
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bioRxiv - Genomics 2020Quote: ... The lysates were sonicated with 5 × 5 min with 5s on/off intervals (Bioruptor, Diagenode) to get the DNA lengths between 200 and 1000 bp ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated (5 times 5 min, 30 sec on/off) with a Bioruptor (Diagenode) and pelleted (13000 rpm ...
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bioRxiv - Genomics 2023Quote: ... a Megaruptor 2 (Diagenode) was used to shear 3 μg of DNA to 15kb fragments ...
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bioRxiv - Genomics 2022Quote: ... and chromatin was fragmented into 1–5-kb fragments by sonication (high level, 33 cycles, 30 s ON, 50 s OFF) using a Bioruptor (Diagenode). Chromatin immunoprecipitation was used to enrich the complex fragments with magnetic beads of protein G (Thermo ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Biochemistry 2023Quote: ... for 5 min (Bioruptor Sonicator, Diagenode)73–75 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF, pH 8.5) and sonicated for 5 min (30 sec on, 1 min off) with Bioruptor UCD-200TM (Diagenode, Liège, Belgium). The resulting cell lysates were centrifuged ...
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bioRxiv - Cell Biology 2023Quote: ... H3K27me3 (Diagenode; #C15410195; 2 μg) and IgG-rabbit (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... H3K9me3 (Diagenode; #C15410193; 2 μg), H3K27me3 (Diagenode ...
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bioRxiv - Genomics 2020Quote: ... One µg per 100,000 cells of the desired antibody (H3K4me3 C15410003, H3K27ac C15410196, H3K27me3 C15410195, all from Diagenode) is added into each well ...
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bioRxiv - Molecular Biology 2023Quote: Chromatin-immunoprecipitation (ChIP) for ChIP-qPCR was performed according to the One-day ChIP kit protocol (Diagenode; C01010080). Beads were exchanged to Dynabeads™ Protein A (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Chromatin immunoprecipitation (ChIP) for ChIP-qPCR was performed according to the one-day ChIP kit protocol (Diagenode; C01010080). For each ChIP ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed on ice for 10 minutes prior to sonication for either 20 cycles (replicate 1) or 40 cycles (replicate 2) of 30s on / 30s off with a Bioruptor UCD-200 (Diagenode) at maximum power ...
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bioRxiv - Molecular Biology 2019Quote: ... About 5 μg Ty1 antibody (C15200054; Diagenode), mouse IgG (12-371 ...
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bioRxiv - Developmental Biology 2022Quote: ... One hundred fifty microliters of Hank’s buffered salt solution with 1x protease inhibitor cocktail (in True MicroChIP kit, Diagenode) was added ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sonicated in 6 x 30 second intervals using a Bioruptor 300 Machine (Diagenode), then centrifuged for 10 minutes at 10,000g at 4°C ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... 2 μg anti-H3K27me3 (Diagenode, C15410195), and 2.5 μg anti-enhancer of zeste homolog 2 (EZH2 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 µg of 5mC antibody (Diagenode) were added to the sample and incubated for 2 h at 4°C with rotation ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed from 500 pg total RNA using D-Plex Small RNA-seq Kit for Illumina and Single Indexes for Illumina - Set B (Diagenode, C05030001). RIN aXer cell sorting was determined to be between 1.2 and 7.2 (Bioanalyzer 6000 ...
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bioRxiv - Genomics 2022Quote: ... by boiling at 95°C for 5 minutes followed by 5 sonication cycles of 30 s ON / 30 s OFF on the Bioruptor Pico sonicator (Diagenode) at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 % SDS in PBS) and sonicated 5 times for 5 min (30s ON/ 30s OFF) at high mode in a Bioruptor Pico (Diagenode). IP were performed overnight at 4°C using 1.5 mg of pre-coated epoxy Epoxy Dynabeads (M-270 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 5 minutes with a Bioruptor Pico (Diagenode) to an average fragment size of ∼ 200 bp ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein was extracted by incubating the islets with Laemmli buffer at 95°C for 5 mins and homogenized for another 5 mins in a sonication device (Diagenode, B01060001) at 4°C ...