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Citations for Diagenode :
201 - 250 of 384 citations for 6 Hydroxy 2 2 4 4 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: Membranes disruption was carried by sonication at 4 °C (2x10 min, 30 sec on, 30 sec off, high setting, Diagenode Bioruptor). Extracts were cleared by centrifugation (15,000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... for a total volume of 100 μL and sonicated (4 cycles of 20sec/30sec (on/off time)) using a Bioruptor (Pico II, Diagenode, BE). The successful generation of fragments with a mean fragment size of 400 nucleotides was confirmed using a Bioanalyzer as per manufacturer’s instructions (Agilent ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Biochemistry 2023Quote: Immunoprecipitation was performed using the IP star robot at 4°C (indirect 200 µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10 μg ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... Pelleted cells were resuspended in 1 ml 50 mM Tris pH 7.5 and sonicated in a Bioruptor Twin (Diagenode) on LOW 30 sec ON ...
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bioRxiv - Developmental Biology 2019Quote: ... the amplified cDNA (2 μg per sample) was fragmented to 200 bp using a Bioruptor Sonicator (Diagenode), and barcode ligation and end repair were performed using the Ovation Rapid DR Library System (NuGEN) ...
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bioRxiv - Genetics 2020Quote: ... The DNA was sonicated for 2 cycles of 15sec ON/90 sec OFF in a bioruptor (Diagenode) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... H3K4me3 (Diagenode, C15410003-50): 2μg.
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bioRxiv - Immunology 2022Quote: ... and was sonicated for 4 rounds of 15 cycles of 30 seconds on/30 seconds off on high power (Diagenode Biorupter plus). After sonication ...
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bioRxiv - Microbiology 2019Quote: ... 30 seconds off) for 7 cycles of sonication in a 4°C water bath using the Bioruptor Sonication System (Diagenode, Denville, NJ) at the high-intensity setting ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Plant Biology 2023Quote: ... Slides were blocked with 4% BSA for 30 minutes at 37°C in a moist box followed by primary (anti-H3K9m2, C15410060, Diagenode, 1:500) and secondary (Alexa Fluor 488 Goat anti-Rabbit IgG1 Secondary Antibody ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Chromatin was broken into small pieces with an average size of 0.5-2 kb using the Bioruptor (Diagenode). The sonicated chromatin was then incubated with 5 µg of primary antibodies overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2019Quote: HMW genomic DNA was sheared to 30 kb using a Megaruptor® 2 instrument (Diagenode SA, Seraing, Belgium). DNA-damage and end repair was carried out prior to blunt adaptor ligation and exonuclease purification using ExoIII and ExoVII ...
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bioRxiv - Genomics 2024Quote: ... Clean HMW DNA samples were sheared to a mean size of 20 kb using the Megaruptor 2 (Diagenode), and size checked on the Fragment analyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Chromatin corresponding to 5 μg of DNA was incubated with 2 μg of anti-H3K4me3 (Diagenode; Cat#: C15410003) or anti-H3K9me3 (Diagenode ...
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bioRxiv - Neuroscience 2019Quote: ... Fig.2A) using a closed system ultrasonic disruptor (10 cycles of 30s ON/30s OFF, 4°C; Bioruptor Pico, Diagenode sa, Liege, Belgium). ChIP assays were then conducted using antisera to CTCF (61311 ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
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bioRxiv - Developmental Biology 2024Quote: ... 1% PIC) and sheared to 100-600bp fragments (average 250bp) at 4°C in a Bioruptor® Pico sonication device (Diagenode: Denville, NJ) for 5 cycles (30 sec on ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was fragmented with 2 rounds of 30 cycles 30sec ON-30sec OFF of sonication with Bioruptor Plus (Diagenode) set on HIGH and purified with Qiagen MinElute kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was sheared to a modal size of 10 kb using a Megaruptor 2 (Diagenode Diagnostics, Seraing (Ougrée), Belgium) ...
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bioRxiv - Immunology 2021Quote: ... Anti-GR (C15200010-50, Diagenode), Anti-GAPDH (2275-PC-100 ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-Ser2P (C15200005-50, Diagenode), or anti-Ser5P (C15200007-50 ...
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bioRxiv - Microbiology 2019Quote: ... incubated at 95°C for 10 min and further sonicated for 15 cycles à 30 s at high intensity level and 4°C using Bioruptor®Plus (Diagenode, Liege, Belgium). Protein concentrations were determined by measuring the tryptophan fluorescence at an emission wavelength of 350 nm using 295 nm for excitation with an Infinite® M1000 PRO microplate reader (Tecan ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a maximum of 600 ng of DNA per accession was sheared at 4°C to a target average length of 400 bp using a Bioruptor® Pico sonication device (Diagenode, Denville, NJ, USA). Illumina libraries were prepared using a modified KAPA protocol with the KAPA LTP Library Preparation Kit (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico, Diagenode; 4 cycles of 30s ON + 90s OFF). In both cases ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed and sonicated in the presence of 0.2% SDS for 30 cycles (30 s on, 30 s off) using the high setting on a Bioruptor sonicator (Diagenode). Cell debris were pelleted by centrifugation and SDS in the lysate supernatants was diluted to 0.07% ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ChIPseq was performed as previously described (Bouderlique et al., 2019) using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) and the ThruPLEX DNA-seq (Rubicon Genomics ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were lysed for 15 min on ice with occasional inversion every 2 min and then sonicated for 24 cycles with 30s on and 30s off using Bioruptor (Diagenode). After sonication ...
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bioRxiv - Molecular Biology 2020Quote: ... nucleic acids gently extracted from unfixed NTERA-2 cells were sheared by sonication (12 cycles at high power, 15s ON / 90s OFF) with a Bioruptor (Diagenode). 4.4 μg of sheared nucleic acids were incubated with 10 μg of S9.6 antibody for immunoprecipitation overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Sonication was performed by splitting each sample to 2 Bioruptor tubes with sonication beads and using the Bioruptor Pico (Diagenode) for 18 cycles of 30 sec on and 30 sec off into an average size of approximately 200 bp ...
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bioRxiv - Genomics 2020Quote: ... high molecular weight DNA was diluted to a concentration of 25 ng/uL and fragmented to an average peak size of 25 kb using a Megaruptor-2 instrument (Diagenode). Small fragments <1 kb were removed and the DNA concentrated using bead purification (0.4 x volumes of Ampure-XP beads ...
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bioRxiv - Immunology 2021Quote: ... 2 µg of antibody was used per reaction. H3K9me3 (Lot. A2217P) and H3K4me3 (Lot No. A1052D) antibodies were from Diagenode. Immunoprecipitated and input DNA were extracted by phenol-chloroform extraction and used for qPCR with SsoAdvanced Universal SYBR Green supermix (Biorad) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using phenol/chloroform and 2 µg of purified 3C sample was sonicated using the Bioruptor Pico (Diagenode). Biotinylated DNA was captured using MyOne Streptavidin T1 magnetic beads (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed on ice for 10 minutes prior to sonication for either 20 cycles (replicate 1) or 40 cycles (replicate 2) of 30s on / 30s off with a Bioruptor UCD-200 (Diagenode) at maximum power ...
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bioRxiv - Cancer Biology 2024Quote: Insoluble fractions were further sonicated in 1xPBS with 2% SDS at high intensity using a Bioruptor® Sonication Device (Diagenode) for SDS-PAGE.
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed in 1 ml of extraction buffer by three freeze‒thaw cycles in liquid nitrogen and by sonication (2 cycles, 30 sec high-power pulse) using a Bioruptor Plus (Diagenode). After centrifugation (20,000 x g ...
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bioRxiv - Molecular Biology 2024Quote: ... or anti-Ser5P (C15200007-50, Diagenode). Then beads were washed twice for 15 min with Low Salt and High Salt buffers like for histones but including 1x of PhosSTOP ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-H3K36me3 (1:50, Diagenode C15200183), anti-H3K9me3 (1:50 ...
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bioRxiv - Microbiology 2020Quote: ... was added to the lysate and incubated at 37 °C for 10 min before sonication (2 × 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Microbiology 2022Quote: ... were added to the lysate and incubated at 37 °C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Biochemistry 2022Quote: ... heated to 95°C for 10 min shaking (1000 rpm) followed by 2 cycles à 20 s of sonication in BioRuptor (Diagenode). Aliquots containing 50 μg protein were transferred into a cartridge and cooled ...
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bioRxiv - Genomics 2022Quote: ... HMW-DNA (25 ng/μl) was separately sheared with 30 kb and 35 kb settings using a Megaruptor 2 instrument (Diagenode SA). However ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors) and sonicated using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). Sonicated lysates were diluted 2× in 50mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors and sonication using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). 200μg of proteins for each sample were diluted to 1 ml with CoIP Lysis Buffer and incubated with 30μl of anti-FLAG M2 Magnetic Beads (Sigma M8823 ...
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bioRxiv - Microbiology 2024Quote: ... were added to the lysate and incubated at 37°C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...