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Citations for Diagenode :
201 - 250 of 362 citations for 6 Hydroxy 2 2 4 4 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Plant Biology 2023Quote: ... Slides were blocked with 4% BSA for 30 minutes at 37°C in a moist box followed by primary (anti-H3K9m2, C15410060, Diagenode, 1:500) and secondary (Alexa Fluor 488 Goat anti-Rabbit IgG1 Secondary Antibody ...
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bioRxiv - Systems Biology 2020Quote: ... Chromatin was broken into small pieces with an average size of 0.5-2 kb using the Bioruptor (Diagenode). The sonicated chromatin was then incubated with 5 µg of primary antibodies overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2019Quote: HMW genomic DNA was sheared to 30 kb using a Megaruptor® 2 instrument (Diagenode SA, Seraing, Belgium). DNA-damage and end repair was carried out prior to blunt adaptor ligation and exonuclease purification using ExoIII and ExoVII ...
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bioRxiv - Neuroscience 2019Quote: ... Fig.2A) using a closed system ultrasonic disruptor (10 cycles of 30s ON/30s OFF, 4°C; Bioruptor Pico, Diagenode sa, Liege, Belgium). ChIP assays were then conducted using antisera to CTCF (61311 ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was fragmented with 2 rounds of 30 cycles 30sec ON-30sec OFF of sonication with Bioruptor Plus (Diagenode) set on HIGH and purified with Qiagen MinElute kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was sheared to a modal size of 10 kb using a Megaruptor 2 (Diagenode Diagnostics, Seraing (Ougrée), Belgium) ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples were lysed the same day 2 weeks before proteomics analysis in 100µL iST-NHS-Lysis buffer (PreOmics GmbH) by sonication (Bioruptor (DIagenode) 15s ON/15s OFF for 15 min) ...
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bioRxiv - Immunology 2021Quote: ... Anti-GR (C15200010-50, Diagenode), Anti-GAPDH (2275-PC-100 ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-Ser2P (C15200005-50, Diagenode), or anti-Ser5P (C15200007-50 ...
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bioRxiv - Microbiology 2019Quote: ... incubated at 95°C for 10 min and further sonicated for 15 cycles à 30 s at high intensity level and 4°C using Bioruptor®Plus (Diagenode, Liege, Belgium). Protein concentrations were determined by measuring the tryptophan fluorescence at an emission wavelength of 350 nm using 295 nm for excitation with an Infinite® M1000 PRO microplate reader (Tecan ...
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bioRxiv - Cancer Biology 2023Quote: ... boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico, Diagenode; 4 cycles of 30s ON + 90s OFF). In both cases ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a maximum of 600 ng of DNA per accession was sheared at 4°C to a target average length of 400 bp using a Bioruptor® Pico sonication device (Diagenode, Denville, NJ, USA). Illumina libraries were prepared using a modified KAPA protocol with the KAPA LTP Library Preparation Kit (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed and sonicated in the presence of 0.2% SDS for 30 cycles (30 s on, 30 s off) using the high setting on a Bioruptor sonicator (Diagenode). Cell debris were pelleted by centrifugation and SDS in the lysate supernatants was diluted to 0.07% ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ChIPseq was performed as previously described (Bouderlique et al., 2019) using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) and the ThruPLEX DNA-seq (Rubicon Genomics ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were lysed for 15 min on ice with occasional inversion every 2 min and then sonicated for 24 cycles with 30s on and 30s off using Bioruptor (Diagenode). After sonication ...
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bioRxiv - Molecular Biology 2020Quote: ... nucleic acids gently extracted from unfixed NTERA-2 cells were sheared by sonication (12 cycles at high power, 15s ON / 90s OFF) with a Bioruptor (Diagenode). 4.4 μg of sheared nucleic acids were incubated with 10 μg of S9.6 antibody for immunoprecipitation overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Sonication was performed by splitting each sample to 2 Bioruptor tubes with sonication beads and using the Bioruptor Pico (Diagenode) for 18 cycles of 30 sec on and 30 sec off into an average size of approximately 200 bp ...
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bioRxiv - Genomics 2020Quote: ... high molecular weight DNA was diluted to a concentration of 25 ng/uL and fragmented to an average peak size of 25 kb using a Megaruptor-2 instrument (Diagenode). Small fragments <1 kb were removed and the DNA concentrated using bead purification (0.4 x volumes of Ampure-XP beads ...
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bioRxiv - Immunology 2021Quote: ... 2 µg of antibody was used per reaction. H3K9me3 (Lot. A2217P) and H3K4me3 (Lot No. A1052D) antibodies were from Diagenode. Immunoprecipitated and input DNA were extracted by phenol-chloroform extraction and used for qPCR with SsoAdvanced Universal SYBR Green supermix (Biorad) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using phenol/chloroform and 2 µg of purified 3C sample was sonicated using the Bioruptor Pico (Diagenode). Biotinylated DNA was captured using MyOne Streptavidin T1 magnetic beads (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed on ice for 10 minutes prior to sonication for either 20 cycles (replicate 1) or 40 cycles (replicate 2) of 30s on / 30s off with a Bioruptor UCD-200 (Diagenode) at maximum power ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-H3K36me3 (1:50, Diagenode C15200183), anti-H3K9me3 (1:50 ...
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bioRxiv - Molecular Biology 2024Quote: ... or anti-Ser5P (C15200007-50, Diagenode). Then beads were washed twice for 15 min with Low Salt and High Salt buffers like for histones but including 1x of PhosSTOP ...
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bioRxiv - Microbiology 2020Quote: ... was added to the lysate and incubated at 37 °C for 10 min before sonication (2 × 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Microbiology 2022Quote: ... were added to the lysate and incubated at 37 °C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Biochemistry 2022Quote: ... heated to 95°C for 10 min shaking (1000 rpm) followed by 2 cycles à 20 s of sonication in BioRuptor (Diagenode). Aliquots containing 50 μg protein were transferred into a cartridge and cooled ...
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bioRxiv - Genomics 2022Quote: ... HMW-DNA (25 ng/μl) was separately sheared with 30 kb and 35 kb settings using a Megaruptor 2 instrument (Diagenode SA). However ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors) and sonicated using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). Sonicated lysates were diluted 2× in 50mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors and sonication using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). 200μg of proteins for each sample were diluted to 1 ml with CoIP Lysis Buffer and incubated with 30μl of anti-FLAG M2 Magnetic Beads (Sigma M8823 ...
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bioRxiv - Microbiology 2024Quote: ... were added to the lysate and incubated at 37°C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Cell Biology 2020Quote: ... 600 ng of denatured DNA was incubated with 2 mg of 5-methylcytosine (5-mC) monoclonal antibody ([33D3] Diagenode, Cat No # C15200081) in IP buffer (0.1% SDS ...
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bioRxiv - Genomics 2021Quote: ... Abcam; anti-ERG 2 µg, ab92513, Abcam; anti-H3K4me3 1.4 µg, C15410003, Diagenode; H3K27me3 2.9 µg, C15410069, Diagenode; H3K27ac 1µg, ab4729, Abcam). As wildtype FLI1 or ERG are not expressed in EWSR1-FLI1 or EWSR1-ERG positive EwS cell lines ...
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bioRxiv - Neuroscience 2020Quote: ... H3K4me3 (Diagenode C15410003-50; rabbit; 1 µg), H3K27ac (Abcam ab177178 ...
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bioRxiv - Cancer Biology 2020Quote: ... and H3K4me3 (Cat. No. C15410003-50, Diagenode). Next generation sequencing libraries were prepared using the Microplex Library Preparation kit v2 (Diagenode ...
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bioRxiv - Genetics 2021Quote: ChlPseq was performed on 20k FACS sorted MM cells as previously described 44 using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) but with minor modifications on the preparation of input controls ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-H3K4me3 (Diagenode, C15410003-50; 1:300). Immunocomplexes were captures using Protein-A Sepharose beads CL-4B (GE Healthcare) ...
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bioRxiv - Genomics 2022Quote: N6-methyladenosine (m6A) Antibody (Diagenode C15200082-50 – RRID:AB_2892212), referred hereafter as “DG1”.
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bioRxiv - Genomics 2022Quote: ... and 12 μg of high molecular weight gDNA was sheared to a size distribution between 15 and 20 kbp using the Megaruptor® 2 (Diagenode, Denville, NJ, USA). For each accession ...
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bioRxiv - Neuroscience 2021Quote: ... The following antibodies were used: H3K4me3 (Diagenode C15410003-50), H3K4me1 (Abcam ab8895) ...
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bioRxiv - Immunology 2021Quote: ... and 5 µl Anti-GR antibody C15200010-50 (Diagenode), in a buffer with 1% Triton X-100 and 150 mM NaCl.
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bioRxiv - Cancer Biology 2022Quote: ... and (1:50) delusion of RUNX1/ETO (#C15310197, Diagenode), RUNX1 (#ab35962 ...
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bioRxiv - Neuroscience 2023Quote: ... in presence of 50 mg Protein Extraction beads (C20000021, Diagenode); samples were trypsin digested for 2 h 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and rabbit anti-H3K27me1 (Diagenode, C15410045-50 or Abcam, ab195492). Enrichment of the specific histone mark was quantified by qPCR using SYBR Green Master Mix (Roche ...
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bioRxiv - Systems Biology 2020Quote: ... 50 mM NaCl) and sonicated for 2.5 min (Bioruptor plus, Diagenode). Peptide concentration was estimated by tryptophan assay ...
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bioRxiv - Molecular Biology 2021Quote: ... for 6 cycles (1 min on/1 min off) on a Bioruptor Pico sonicator (Diagenode). For each ChIP reaction 4 % HEK293T-derived human spike-in lysate was combined with mESC lysate and incubated in 1x IP buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sonicated in 6 x 30 second intervals using a Bioruptor 300 Machine (Diagenode), then centrifuged for 10 minutes at 10,000g at 4°C ...
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bioRxiv - Genomics 2021Quote: ... the DNA was sheared to approximately 50 kb using a BioRuptor (Diagenode) and pre-sized using the >20-kb high-pass broad-range protocol on a PippinHT (Sage Science) ...