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Citations for Diagenode :
201 - 250 of 253 citations for 3 Acetyl 1H indole 4 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... were sonicated with 5 cycles of 30 s on followed by 30 s off at 4°C (Bioruptor Plus, Diagenode). After the centrifugation for 15 min at 4°C and 16,100 ×g ...
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bioRxiv - Molecular Biology 2024Quote: ... for 5 min at 4°C and lysates were sonicated (1x 5 min, 30 sec on/off) with a Bioruptor (Diagenode) followed by digestion with 40U RNaseI (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... sonicated for 10 cycles with 30 ‘’ on and 30 ‘’ off at maximum power at 4 °C using a Bioruptor Pico sonication device (Diagenode). Afterwards ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were sonicated at 4°C for 10 min (30 sec ON, 30 sec OFF) at medium frequency (Bioruptor Diagenode). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... and sonicated (6 cycles, high power for 30 s on and 30 s off at 4 °C, Bioruptor NextGen (Diagenode)) ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were sonicated using 40 cycles of 15s on/off at 4°C in the Bioruptor plus (model UCD-300, Diagenode). Further ...
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bioRxiv - Microbiology 2023Quote: ... was dissolved in 75 µL lysis buffer, boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). Lysates from the secretomes and intracellular fractions were centrifuged (10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... chromatin was sheared by sonication into 500 – 1000pb fragment (30sec ON/30sec OFF, 6 cycles, 4°C, Diagenode Bioruptor Pico). 10µl was kept aside as the input ...
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bioRxiv - Cell Biology 2022Quote: ... cells were sonicated for 10 cycles of 30 sec “on” and 30 sec “off” at 4°C with Bioruptor (Diagenode) and the protein concentration was measured using Bradford protein assay (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were centrifuged at 14000rpm for 15mins at 4 oC and precleared using with 4ml of Magnetic A Beads (Diagenode) at 4 oC for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were sonicated for 4 min on a 30 s on/30 s off cycle using a Bioruptor Pico (Diagenode). Subsequent steps were performed as in the Universal Plant ChIP-seq kit protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were sonicated using 40 cycles of 15s on/off at 4 °C in the Bioruptor plus (model UCD-300, Diagenode) after boiling at 99 °C for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were extracted by sonication executing 40 cycles of 15s on and 15 s off at 4 °C using a Bioruptor plus (model UCD-300, Diagenode). After lysis ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then subjected to five cycles (30 seconds on/30 seconds off) of sonication at 4°C on high intensity in a Bioruptor Sonication System (Diagenode) and subsequently cleared by centrifugation at 14,000g for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed overnight at +4°C with primary antibodies (H3K4me2 1:1000; Abcam ab32356) (H3K4me3 1:200; Diagenode C15410003) (H3K4me2 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... DNA was sonicated at 4°C (program: 6 cycles of 30 s on, 60 s off) on a Bioruptor Plus (Diagenode), achieving a size distribution between 200-1000 nucleotides (nt) ...
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bioRxiv - Neuroscience 2024Quote: ... on ice for 15 minutes and subjected to sonication for 4 separate cycles of 30s on and 30s off cycles at high setting (Bioruptor Plus, Diagenode). The resultant chromatin was centrifuged at 12000 rpm for 30 minutes to remove any debris ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were incubated overnight at 4°C with rotation in wash buffer with rabbit anti-Chd1 (Diagenode 15410334, 1:50). Nuclei were incubated for 1 hour at room temperature with rotation with rabbit anti-H3K4me3 (Sigma-Aldrich 05-745R ...
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bioRxiv - Cancer Biology 2024Quote: ... The suspension was sonicated for 15 cycles (30 seconds on/ 30 seconds off) at 4 ◦C (Diagenode Pico Bioruptor sonicator). Chromatin concentration was quantified using the Qubit 4 Fluorometer and Qubit dsDNA Broad Range kit ...
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bioRxiv - Neuroscience 2024Quote: ... Cell extracts were then sonicated at 4°C for 5 cycles (30 seconds on / 30 seconds off) with the Bioruptor Pico (Diagenode), prior to proceeding with protein quantification using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 x 106 flow-sorted chromosomes were pelleted at 10,000 g for 10 min at 4 °C before tagmentation in a 50 μl reaction (2.5 μl Tagmentase (loaded Tn5 transposase, Diagenode, C01070012), 25 μl 2X Tagmentation buffer (Diagenode ...
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bioRxiv - Plant Biology 2024Quote: ... Twenty µl was saved as input and the remaining supernatant was then incubated at 4°C overnight with specific antibodies (5 µg of H3K27me3 Ab, Diagenode C15410069 or Millipore 07-449 ...
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bioRxiv - Plant Biology 2023Quote: ChIP assays were performed on leaves and axillary buds enriched samples of 3 weeks old plants using anti-H3K27me3 (Diagenode pAb-195-050) and anti-IgG (Abcam ab6702) ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mM TEAB and 1 x Halt protease inhibitor cocktail) and sonication (3 × 5 min cycles of 30s on/off; Diagenode Bioruptor sonication device). Extracted proteins protein were digested to peptides with in-solution digestion performed at the Turku Proteomics Facility ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was then de-crosslinked and eluted for 4 hours at 65C before being purified using IPure beads (Diagenode Cat# C03010014). The purified DNA was then subjected to sequencing library preparation or qPCR analysis ...
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bioRxiv - Plant Biology 2021Quote: ... The IP of the methylated DNA was performed overnight at 4°C using Protein A Dynabeads coated with anti-5mC (Diagenode, C15200081) or anti-IgG (Diagenode ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were incubated by rotating overnight at 4°C with primary antibody or irrelevant IgGs and then incubated with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 3 hr at 4 °C with rotation ...
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bioRxiv - Biochemistry 2022Quote: ... samples were sonicated at 4°C in 30 s on/off intervals for 15 cycles using a Bioruptor ® Plus sonication instrument (Diagenode) at high-intensity settings ...
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bioRxiv - Systems Biology 2021Quote: SARS-CoV-2 infected A549-ACE2 cell lysates were sonicated (10 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). Protein concentration was adjusted based on Pierce660 assay supplemented with ionic detergent compatibility reagent ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). HA or Streptactin beads were added to cleared lysates and samples were incubated for 3 hours at 4°C under constant rotation ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). Following normalization of protein concentrations of cleared lysates ...
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bioRxiv - Cell Biology 2021Quote: ... The homogenized samples were submitted to 10 cycles of 10 s at maximal intensity at 4⁰C in a sonicator bath (Bioruptor, Diagenode, USA). Then the homogenates were centrifuged at 10,000 g for 10 min at 4⁰C and the supernatants (NP40-soluble fraction ...
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bioRxiv - Genomics 2021Quote: ... pH 7.4) aided by homogenization by passing the cells through various gouge needles and sonication using the Bioruptor® sonication system (Diagenode) for 15 cycles of 30 s ON / 30 s OFF at high setting ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... for a total volume of 100 μL and sonicated (4 cycles of 20sec/30sec (on/off time)) using a Bioruptor (Pico II, Diagenode, BE). The successful generation of fragments with a mean fragment size of 400 nucleotides was confirmed using a Bioanalyzer as per manufacturer’s instructions (Agilent ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Biochemistry 2023Quote: Immunoprecipitation was performed using the IP star robot at 4°C (indirect 200 µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10 μg ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: Membranes disruption was carried by sonication at 4 °C (2x10 min, 30 sec on, 30 sec off, high setting, Diagenode Bioruptor). Extracts were cleared by centrifugation (15,000 rpm ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 10.0 μg of high molecular weight purified genomic DNA (final volume of 120 μl) was sheared using the Diagenode Megaruptor 3 instrument (Diagenode Inc., Denville, NJ, USA) and a two-cycle shear method at speed 33 followed by speed 34 ...
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bioRxiv - Immunology 2022Quote: ... and was sonicated for 4 rounds of 15 cycles of 30 seconds on/30 seconds off on high power (Diagenode Biorupter plus). After sonication ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Slides were blocked with 4% BSA for 30 minutes at 37°C in a moist box followed by primary (anti-H3K9m2, C15410060, Diagenode, 1:500) and secondary (Alexa Fluor 488 Goat anti-Rabbit IgG1 Secondary Antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
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bioRxiv - Genomics 2024Quote: ... at 4°C for 13 cycles with 30 sec on and 30 sec rest in 15 ml Bioruptor Tubes (Diagenode Cat #C30010017) with 650 mg of beads (Diagenode Cat #C03070001) ...
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bioRxiv - Neuroscience 2024Quote: ... and sonicated for 15 min (cycles of 30 sec ON and 30 sec OFF, 4°C, low frequency) in a Bioruptor Pico (Diagenode, Seraing, Belgium). Samples were subsequently incubated for 15 min at 70°C ...
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bioRxiv - Immunology 2024Quote: ... by sonication for 15 min (30 s on/off cycles) at 4 °C with high power in a Bioruptor device (Diagenode, Liège, Belgium). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... We transferred cells into 1.5 ml sonication tubes (200 µL/tube) and sonicated 30 sec on/30 sec off for 6 cycles at 4 °C using Bioruptor® Pico (Diagenode, Denville, NJ). We centrifuged fragmented chromatin at 20,800 g for 10 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
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bioRxiv - Developmental Biology 2024Quote: ... 1% PIC) and sheared to 100-600bp fragments (average 250bp) at 4°C in a Bioruptor® Pico sonication device (Diagenode: Denville, NJ) for 5 cycles (30 sec on ...