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Citations for Diagenode :
151 - 200 of 430 citations for Rat Small Nuclear Ribonucleoprotein U5 Subunit 200 SNRNP200 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: MeDIP-Seq libraries for Ion Torrent semiconductor sequencing were performed on the Ion Torrent PGM using a modified protocol from Ion Plus Fragment Library kit (Catalog # 4471252) combined with a 5-hydroxymethylcytosine (5hmC Kit, Catalog # AF-110–0016) immunoprecipitation kit (Diagenode) according to a previously optimized protocol (Guerrero-Bosagna & Jensen ...
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bioRxiv - Immunology 2022Quote: ... iPure kit v2 (Diagenode) was used ...
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bioRxiv - Immunology 2021Quote: ... iPure kit v2 (Diagenode) was used ...
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bioRxiv - Immunology 2020Quote: ... Cellular lysates were sonicated for 40 minutes (30sec ON, 30sec OFF) on high in a Bioruptor UCD-200 (Diagenode). After sonication ...
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bioRxiv - Developmental Biology 2024Quote: ... Chromatin was sonicated in a size range of 200– 500 bp by using the Bioruptor UCD-300 system (Diagenode). CTCF-bound chromatin was immunoprecipitated by using the iDeal Kit for Transcription Factors (C01010055 ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was purified according to the IPure kit v2 Kit manual (Diagenode, C03010015). The amount of immunoprecipitated DNA was quantified by qPCR ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then sonicated until DNA was fragmented to an average distribution of about 200–300 bp using a Bioruptor (Diagenode) for a total of 30 cycles (30s on and 30s off) ...
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bioRxiv - Genomics 2020Quote: ... samples were sonicated 23 cycles (each cycle: 30 seconds on and 30 seconds off) with a Bioruptor UCD-200 (Diagenode). Lysates were precleared with agarose beads (BBI no ...
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bioRxiv - Genetics 2020Quote: ... The top 100 µl sperm suspension was sonicated at the medium level for five times with 3 seconds each (Bioruptor UCD-200; Diagenode). An aliquot of 2 µl sperm HTF suspension was mixed immediately with 50 µl of 4% PVP (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was transferred to a sterile 2 ml microcentrifuge tube and sonicated with the Bioruptor UCD-200 sonicator (Diagenode) for 1 min on the low setting (160W at 20kHz ...
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bioRxiv - Biochemistry 2022Quote: ... and chromatin was sheared to an average size of 200-600 bp by sonication for 20 cycles 30 sec on/30 sec off using Bioruptor Pico (Diagenode). 1% Triton X-100 was added after sonication ...
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bioRxiv - Neuroscience 2022Quote: ... cells were cross-linked with 1% paraformaldehyde for 15 minutes and Chromatins were sheared into an average 200–400 bp in length by sonication (Diagenode) and immunoprecipitated with following antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells and nuclei were subsequently lysed and chromatin was sonicated to fragment DNA to about 200-500 bp on a Bioruptor Pico sonication device (Diagenode). Sonicated chromatin was pre-cleared with same-host IgG and protein G Dynabeads (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromatin was then sonicated using 30sec on/off cycles for 25 minutes in the BiorupterTM UCD-200 (Diagenode, Liège, Belgium) sonicator to a mean sheared length of ~500kb ...
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bioRxiv - Molecular Biology 2021Quote: ... by sonication for four cycles of 30 s pulses with low power and 60 s cooling between pulses using a Bioruptor UCD-200 (Diagenode). Following centrifugation (16,100g for 10 min at 4°C) ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was purified by phenol-chloroform treatment followed by ethanol precipitation and then fragmented by sonication with a model UCD-200 Bioruptor (Diagenode). After the biotin-labeled DNA was pulled down with Dynabeads Streptavidin T1 (65601 ...
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bioRxiv - Molecular Biology 2023Quote: ... and sonicated for 10 min (medium intensity, 30 s on/30 s off) in a Bioruptor UCD-200 system (Diagenode), and centrifuged at 21,000 x g for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Cross-linked cell lysates were solubilized using a multi-beads shocker (Yasui Kikai) and sonicated using a Bioruptor UCD-200 system (Diagenode). The sheared samples were centrifuged at 20,000 g for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... were sonicated 8 pulses (30 seconds ON / 30 seconds OFF) to 200–500 bp fragments using a Bioruptor Pico sonicator (Diagenode) in 200ul of L3 buffer ...
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bioRxiv - Plant Biology 2023Quote: ... pH 8.0 and protease inhibitor cocktail) and sheared into 200-500 bp fragments with a sonicator (Diagenode, Bioruptor Plus, Belgium). The sonicated chromatin complex was immunoprecipitated by anti-H2A.Z and isolated by binding to Dynabeads Protein A/G (26162 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed on ice for 10 minutes prior to sonication for either 20 cycles (replicate 1) or 40 cycles (replicate 2) of 30s on / 30s off with a Bioruptor UCD-200 (Diagenode) at maximum power ...
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bioRxiv - Cell Biology 2022Quote: 10 μg of genomic DNA were sonicated during 40 cycles (30 s ON/60 s OFF, low intensity) into 200-400 bp fragments using a Bioruptor Plus sonicator (Diagenode). Sonication efficiency was verified by electrophoresis in a 1.5% agarose gel ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed overnight at +4°C with primary antibodies (H3K4me2 1:1000; Abcam ab32356) (H3K4me3 1:200; Diagenode C15410003) (H3K4me2 1:1000 ...
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bioRxiv - Immunology 2023Quote: ... or ChIP proceeded by sonicating with for 40 min (30 sec on/off 10 min cycles) in a Bioruptor UCD-200 (Diagenode). Lysates were cleared and 10% taken for input samples.
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bioRxiv - Cancer Biology 2023Quote: ... Cross-linked chromatin was isolated and sonicated to generate DNA fragments averaging 200-500 bp in length by Bioruptor plus sonication device (Diagenode). Chromatin fragments were immunoprecipitated with antibodies directed against ZEB1 (1 μg ...
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bioRxiv - Developmental Biology 2023Quote: ... was added per sample to digest cell walls and samples were incubated for another 15 min at 4°C before sonicated in an ice bath 4x for 30 s on high setting using a Bioruptor UCD-200 (Diagenode) with 90 sec breaks on ice ...
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bioRxiv - Plant Biology 2024Quote: ... The extracts were then distributed into 1.5 ml reaction tubes and sonicated in an ice bath four times for 30 seconds on a high setting using a Bioruptor UCD-200 (Diagenode) with 1.5 minutes breaks ...
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bioRxiv - Developmental Biology 2024Quote: ... Chromatin was then isolated by lysis and sonicated to an average size of 200– 500 bp using a Bioruptor (Diagenode). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... or hMeDIP kit (Diagenode, C02010031). Quantification of precipitated DNA was determined using real-time PCR and the delta Ct method for relative quantitation (ABI 7900HT Fast Real-Time PCR System).
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bioRxiv - Genetics 2024Quote: ... Premium Bisulfite kit (Diagenode, USA), Fast BC kit (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was passed through an 18–21-gauge needle and submitted to sonication using the Bioruptor VCD-200 (Diagenode, Belgium). After centrifugation for 10 min at 20,000 x g at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 200 mM NaCl) supplemented with 100 μg/ml ssDNA and PI and sonicated using Bioruptor Pico sonicator (Diagenode, Denville, NJ, USA). In order to reach DNA fragments in the range of 200-700 bp ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM EDTA and 100 mM NaCl) were added to the nuclei pellet and sonicated with the BioRuptor UCD-200 (Diagenode, Belgium) for 25 cycles at high power and 30 s intervals ...
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bioRxiv - Developmental Biology 2024Quote: ... The TRIM66 antibody (Tif1δ, PG124) (Khetchoumian et al, 2004) dilution was 1:250 and for all histone modifications was 1:200 (H3K4me3 (Diagenode, C15410003), H3K18Ac (Abcam ...
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bioRxiv - Biochemistry 2023Quote: Immunoprecipitation was performed using the IP star robot at 4°C (indirect 200 µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10 μg ...
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bioRxiv - Microbiology 2024Quote: ... and the samples were heated for 5 min at 95°C prior to sonication with a Bioruptor UCD-200 (Diagenode, Seraing, Belgium) device for ten cycles of 1 min pulse and 30 sec pause at high power ...
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bioRxiv - Molecular Biology 2021Quote: ... 1μL SYBR green 10X (Diagenode kit), 1.3 μL of molecular biology grade water and 5μL of 2xPCR NEB master mix ...
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bioRxiv - Genetics 2020Quote: ... using the LowCell# ChIP kit (Diagenode). Illumina sequencing libraries were generated using the Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences) ...
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bioRxiv - Developmental Biology 2022Quote: ... and the iDeal ChIP-kit (Diagenode) for MED1 according to the manufacturer’s protocols with some modifications as described below ...
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bioRxiv - Genomics 2024Quote: ... using a Microplex v3 kit (Diagenode).
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bioRxiv - Genomics 2024Quote: ... the µChipmentation kit for histones (Diagenode) was used for chromatin immunoprecipitation and ChIP-Seq library preparation (Supplementary table 2).
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bioRxiv - Developmental Biology 2023Quote: ... the iDeal ChIP-Seq kit (Diagenode) was used according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 30 s by 30 s at mild intensity and 5 times for differentiating myoblasts using a Bioruptor UCD-200 (Diagenode, Denville, NJ, USA). Then the samples were incubated with the anti-MTF1 antibody or IgG as a negative control[52] ...
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bioRxiv - Molecular Biology 2021Quote: ... the Microplex Library Preparation kit V2 (Diagenode) was used for ChIP-Seq library construction following the manufacturer recommendations ...
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bioRxiv - Genomics 2021Quote: The protocol for Premium RRBS kit (Diagenode) library preparation included enzymatic digestion of 100 ng of gDNA with MspI ...
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bioRxiv - Immunology 2020Quote: ... DNA was purified using IPure kit (Diagenode) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... using the iDeal ChIP-seq kit (Diagenode). Libraries were prepared using the Ovation Ultralow V2 DNA-Seq library preparation kit (NuGen ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries prepared using the MicroPlex kit (Diagenode) were pooled and sequenced at a depth of approximately 30 M on an Illumina HiSeq 2500 at the Michigan State University (MSU ...
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bioRxiv - Developmental Biology 2024Quote: ... and Chromatin EasyShear Kit - High SDS (Diagenode) and the Fish’n ChIPs (Chromatin Immunoprecipitation in the Zebrafish Embryo ...
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bioRxiv - Genomics 2024Quote: ... and one of two U2OS libraries were prepared by first treating 3 μg of DNA with the Short Fragment Eliminator Kit (ONT) according to the manufacturer’s protocol before shearing with the DNA Fluid+ Kit on a Megaruptor 3 (Diagenode) to a mean size of approximately 50k bp ...