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Citations for Diagenode :
151 - 200 of 245 citations for Potassium 3 4 difluorophenyl trifluoroborate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... were subjected to 3 cycles of sonication (30sec ON/30sec OFF for 10 min, at 200W, high setting, Bioruptor, Diagenode). Triton X-100 was added to the sonicated lysate to a final concentration of 1% ...
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bioRxiv - Microbiology 2024Quote: ... The cells were incubated for 20 min on ice and lysed by sonication (3 cycles, 30 sec high-power pulse) using a Bioruptor Plus (Diagenode). After centrifugation (10,000 x g ...
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bioRxiv - Genetics 2024Quote: ... We sheared the high molecular weight (HMW) DNA to achieve consistent and narrow size ranges to 20kb using the Megaruptor 3 Shearing kit (E07010003, Diagenode). Megaruptor 3 was performed using customized settings ...
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bioRxiv - Genomics 2020Quote: Preparation of reagents: ChIP Wash buffers 1 to 4 (from iDeal ChIP-seq kit for histones, Diagenode C01010173) are aliquoted into quarter module reservoirs divided by length ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154; H3K27me1: Diagenode: C15410045-50) diluted in 1% BSA ...
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bioRxiv - Developmental Biology 2024Quote: ... The sonication of samples was then performed at 4°C using a Bioruptor® Plus sonication device (Diagenode) at high-intensity settings ...
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bioRxiv - Molecular Biology 2024Quote: ... They were subsequently sonicated for 4 cycles (30 sec on, 30 sec off) using the Bioruptor Pico (Diagenode) and centrifuged for 15 min at 14,000 x g ...
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bioRxiv - Physiology 2024Quote: ... and 500 µl volume) in a water bath sonicator set at 4°C (Bioruptor 300. Diagenode, Denville, NJ). After centrifuging at 10,000g for 10 min at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... A HiFi library was then prepared according to the “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” manual with an initial DNA fragmentation by Megaruptor 3 (Diagenode, Belgium) and final library size binning into defined fractions by SageELF (Sage Science ...
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bioRxiv - Cancer Biology 2024Quote: ... buffer with added protease and phosphatase inhibitors and sonicated on high for 30 seconds off 30 seconds on for 3 minutes (Diagenode Bioruptor). The protein concentration was determined using the Micro BCA TM protein assay kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... An input of 50 ng of genomic DNA was sheared to 6 kb - 10 kb using the Megaruptor® 3 (Diagenode) or g-TUBE (Covaris) ...
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bioRxiv - Plant Biology 2024Quote: ... 65µg of genomic DNA was split into 5 aliquots and manually sheared with the Megaruptor 3 instrument (Diagenode, P/N B06010003), according to the Megaruptor 3 operations manual ...
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bioRxiv - Genetics 2021Quote: ... ONT libraries were prepared using the EXP-NBD103 and SQK-LSK109 kits according to the manufacturer’s instructions and using 4 µg of 40Kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Genomics 2020Quote: ... For single crosslinked cells nuclei were sonicated for 4 cycles of 30s on/30s off using a Picoruptor (Diagenode). Immunoprecipitation was carried out overnight at 4°C using 2 μg of RUNX1 antibody (ab23980 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitation was done with 2 µg of chromatin and 4 µg of antibody against H3K9me3 (Diagenode, C15410193, lot A2217P). Sequencing libraries were prepared from 5 ng of immunoprecipitated DNA as previously described [19 ...
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bioRxiv - Microbiology 2023Quote: ... 40 mM 2-Chloroacetamide, 50 mM HEPES pH 8.5), boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). For the intracellular proteomes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with mixing for 30 min at 4°C before sonicating with a Bioruptor® Pico (Diagenode). Lysates were then spun for 10 min at 15,000 g ...
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bioRxiv - Plant Biology 2024Quote: ... Primary antibody incubation was performed overnight at 4°C with gentle shaking using anti-H3K9me2 (Diagenode Cat. No. C15410060), Anti-Histone H3 (Sigma-Aldrich Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then incubated at 4°C overnight with or without 12.5 μg of anti-5hmC antibody (C15220001, Diagenode) in freshly prepared 1X Immunoprecipitation (IP ...
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bioRxiv - Immunology 2022Quote: ... Sonicated chromatin was diluted ten times in ChIP Dilution Buffer and immunoprecipitation was done overnight at 4°C by incubating 2.5 μl (7 μg) of anti-H3K27ac antibody (C15410196; Diagenode) precoated on 25-μl protein A-coated magnetic beads (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Chromatin was fragmented by sonication at 4°C using a Bioruptor 300 (Diagenode; 45 cycles, 30s on/30s off). Immunoprecipitation used 4 μg ChIP validated antibody against NF-κB p65 (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were sonicated for 5 cycles of 30 s on/off (2.5 min total sonication time) at 4°C in a Bioruptor Plus sonication system (Diagenode) and pelleted by centrifugation at 8000 RPM for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Following lysis, cell lysates were sonicated for 4 cycles (30s on/30s off, high setting) using a Bioruptor (Diagenode), followed by a 15-min incubation on ice ...
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bioRxiv - Cancer Biology 2024Quote: Hi-C libraries were built from 4 µg of DNA per sample and sonicated using a BioruptorPico device (Diagenode, cat ...
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bioRxiv - Molecular Biology 2024Quote: ... native ChIP was performed using an increasing amount of H3K4me3 antibodies (for the Abcam 0, 2, 4, 6, 10μl, for the Diagenode 0 ...
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bioRxiv - Developmental Biology 2021Quote: ... and subsequently sonicated for 3 cycles (30 seconds ON, 30 seconds OFF) on low settings using a Bioruptor® Plus (Diagenode, #B01020001). Samples were then centrifuged for 10 min at 10.000 g and supernatant was collected ...
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bioRxiv - Cancer Biology 2021Quote: ... lysed in 1% SDS containing buffer supplemented with 1x protease and phosphatase inhibitors, and sonicated for 4 x 10 cycles (30 sec on, 30 sec off) in temperature-controlled Bioruptor 300 (Diagenode). Debris was removed by centrifuging at 14,000 rpm at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... was then added at a final concentration of 1 % for 15 min at RT and samples were sonicated over 20 min (10 × 30 s pulses) at 4°C to cleave genomic DNA (Bioruptor, Diagenode). Anti-MAZ antibody was from Bethyl Laboratories (A301-652A) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split into 150 μl aliquots in 0.5 ml Eppendorf tubes and sonicated at 4°C for 30 cycles (30 seconds on/off) using a Bioruptor (Diagenode). Aliquots of the same samples were pooled and spun down at 12,000xg for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitation was performed using IP star robot at 4°C (indirect 200µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10μg ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 95°C for 5 min and sonicated at 4°C for 2 cycles of 20 s at high intensity level using a BioRuptor (Diagenode). Samples were clarified by centrifugation at 16,000 x g for 15 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were sonicated at 4° C for 20 minutes using a waterbath sonicator cycling 30 seconds on and 30 seconds off (Bioruptor Pico, Diagenode). Sonicated lysates were cleared by centrifugation at 18,000 g for 10 minutes at 4° C ...
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bioRxiv - Molecular Biology 2020Quote: ... with minor modifications: Cells were resuspended in PBS at 4°C after flow cytometry sorting and incubated with ChIP Crosslink Gold (Diagenode #C01019027 ...
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bioRxiv - Genomics 2021Quote: ... Lysates were sonicated for 15 cycles (30 sec on / 30 sec off) at 4°C using a Bioruptor Pico sonicator (Diagenode). After sonication ...
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bioRxiv - Cell Biology 2023Quote: ... which were incubated overnight on a rotatory wheel at 4°C with the primary antibodies H4K20me1 (Diagenode; #C15410034; 2 μg), H3K9me3 (Diagenode ...
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bioRxiv - Cancer Biology 2023Quote: ... For protein extractions, the chromatin fraction was sonicated for 4 min (30s-on/30s-off, “high power”) on the Bioruptor Plus (Diagenode). Proteins’ content was quantified with the Thermo Fisher Scientific− BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Genomics 2023Quote: Two micrograms of genomic DNA were sonicated for 6 cycles (6 s on, 90 s off) at 4 °C with a Bioruptor NGS (Diagenode), generating average fragments of 1 kb ...
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bioRxiv - Neuroscience 2023Quote: ... Overnight incubation with primary antibodies at 4°C was performed using the following antibodies: Cas9 (Diagenode, C15200230-100, 1:500), alpha synuclein (Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell suspensions in 0.6 mL µtube were sonicated in water bath at 4°C during 12 min (15 sec. ON, 15 sec. OFF) with a Bioruptor apparatus (Diagenode) setted on high power and then clarified by 10 min centrifigation at 10 000 rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... were sonicated with 5 cycles of 30 s on followed by 30 s off at 4°C (Bioruptor Plus, Diagenode). After the centrifugation for 15 min at 4°C and 16,100 ×g ...
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bioRxiv - Molecular Biology 2024Quote: ... for 5 min at 4°C and lysates were sonicated (1x 5 min, 30 sec on/off) with a Bioruptor (Diagenode) followed by digestion with 40U RNaseI (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... sonicated for 10 cycles with 30 ‘’ on and 30 ‘’ off at maximum power at 4 °C using a Bioruptor Pico sonication device (Diagenode). Afterwards ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were sonicated at 4°C for 10 min (30 sec ON, 30 sec OFF) at medium frequency (Bioruptor Diagenode). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... and sonicated (6 cycles, high power for 30 s on and 30 s off at 4 °C, Bioruptor NextGen (Diagenode)) ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were sonicated using 40 cycles of 15s on/off at 4°C in the Bioruptor plus (model UCD-300, Diagenode). Further ...
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bioRxiv - Microbiology 2023Quote: ... was dissolved in 75 µL lysis buffer, boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). Lysates from the secretomes and intracellular fractions were centrifuged (10 min ...