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Citations for Diagenode :
151 - 200 of 341 citations for Monoisononyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: HMW genomic DNA was sheared to 30 kb using a Megaruptor® 2 instrument (Diagenode SA, Seraing, Belgium). DNA-damage and end repair was carried out prior to blunt adaptor ligation and exonuclease purification using ExoIII and ExoVII ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM EDTA and 100 mM NaCl) were added to the nuclei pellet and sonicated with the BioRuptor UCD-200 (Diagenode, Belgium) for 25 cycles at high power and 30 s intervals ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was diluted to 100 ng/uL (10,000 ng total) and sheared to approximately 500 bp using a BioRupter (Diagenode, Denville, NJ). We performed a double-sided size selection using SPRI beads (46 ...
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bioRxiv - Microbiology 2023Quote: ... The DNA was diluted to 100 µL at a concentration of 10 ng / µL and then sheared using a Biorupter® Pico (Diagenode) with 13 cycles of 30 s on followed by 30 s off and quantified using an Agilent D1000 ScreenTape (Agilent Technologies) ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2023Quote: ... The chromatin was then sheared to 300-500 bp using a water bath sonicator (Diagenode SA, Picoruptor, BC 100, LAUDA Germany) for 30 cycles of 30 s ON / 30 s OFF at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... for a total volume of 100 μL and sonicated (4 cycles of 20sec/30sec (on/off time)) using a Bioruptor (Pico II, Diagenode, BE). The successful generation of fragments with a mean fragment size of 400 nucleotides was confirmed using a Bioanalyzer as per manufacturer’s instructions (Agilent ...
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bioRxiv - Genomics 2021Quote: ... 0.5 % NP-40) and evenly split into pre-cooled 1.5 ml Bioruptor tubes (Diagenode). After sonication (15 sec on/ 30 sec off ...
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bioRxiv - Immunology 2020Quote: 1.5 ml Bioruptor Microtubes were filled with 250 mg of Protein Extraction Beads (Diagenode) and filled with RIPA buffer (supplemented with protease and phosphatase inhibitors) ...
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bioRxiv - Neuroscience 2021Quote: ... 1mg/mL BSA) and sonicating at full strength for 15 min (Diagenode, Bioruptor Plus). Protein concentration was measured using a Bradford Assay and normalized so that all assay inputs contained the same amount of protein ...
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bioRxiv - Genetics 2022Quote: ... per 10 ml of solution and lysed by sonication in Bioruptor (UCD-200, Diagenode) with the following settings ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were transferred into 15-ml TPX tubes and sonicated using Bioruptor Plus (Diagenode) with the following parameters ...
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bioRxiv - Biochemistry 2021Quote: Cellular human and viral material in sputum deposited on glass slides was lysed in 50 μl 2% SDS dissolved in 50 mM Tris/HCl (pH 8.2) followed by sonication in a Bioruptor Pico (Diagenode). Proteins were digested using the SP3 protocol as described below.
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bioRxiv - Cell Biology 2021Quote: ... DNA was fragmented with 2 rounds of 30 cycles 30sec ON-30sec OFF of sonication with Bioruptor Plus (Diagenode) set on HIGH and purified with Qiagen MinElute kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was sheared to a modal size of 10 kb using a Megaruptor 2 (Diagenode Diagnostics, Seraing (Ougrée), Belgium) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples were lysed the same day 2 weeks before proteomics analysis in 100µL iST-NHS-Lysis buffer (PreOmics GmbH) by sonication (Bioruptor (DIagenode) 15s ON/15s OFF for 15 min) ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitation was done with 2 µg of chromatin and 4 µg of antibody against H3K9me3 (Diagenode, C15410193, lot A2217P). Sequencing libraries were prepared from 5 ng of immunoprecipitated DNA as previously described [19 ...
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bioRxiv - Cell Biology 2023Quote: ... H3K9me3 (Diagenode; #C15410193; 1:1000) and H3K27me3 (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... H4K20me1 (Diagenode; #C15410034; 1:500), H3K9me3 (Diagenode ...
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bioRxiv - Developmental Biology 2023Quote: ... H3K4me3 (Diagenode #C15410003, 1 μg), H3K27me3 (Diagenode #C15410069 ...
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bioRxiv - Cell Biology 2023Quote: ... H3K4me3 (1:1000; Diagenode C15410003), H3S10ph clone MC463 (1:2000 ...
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bioRxiv - Genetics 2023Quote: ... H3K79me1 (1:1000; Diagenode, C15410082), H3K79me2 (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sox4 (1:4000, Diagenode #C15310129), GAPDH (1:4000 ...
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bioRxiv - Neuroscience 2024Quote: ... H3K27me3 (Diagenode, C1541095, 1:2000), Total H3 (Cell Signaling ...
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bioRxiv - Biochemistry 2020Quote: ... 12 μg of genomic DNA was sonicated into fragments ranging between 100 and 500 basepairs (bp) (checked by agarose gel electrophoresis) with a BioRuptor (Diagenode, Liège, Belgium). 25% of the samples was saved as input ...
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bioRxiv - Molecular Biology 2021Quote: ... for 6 cycles (1 min on/1 min off) on a Bioruptor Pico sonicator (Diagenode). For each ChIP reaction 4 % HEK293T-derived human spike-in lysate was combined with mESC lysate and incubated in 1x IP buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed and sonicated in the presence of 0.2% SDS for 30 cycles (30 s on, 30 s off) using the high setting on a Bioruptor sonicator (Diagenode). Cell debris were pelleted by centrifugation and SDS in the lysate supernatants was diluted to 0.07% ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ChIPseq was performed as previously described (Bouderlique et al., 2019) using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) and the ThruPLEX DNA-seq (Rubicon Genomics ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were lysed for 15 min on ice with occasional inversion every 2 min and then sonicated for 24 cycles with 30s on and 30s off using Bioruptor (Diagenode). After sonication ...
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bioRxiv - Molecular Biology 2020Quote: ... nucleic acids gently extracted from unfixed NTERA-2 cells were sheared by sonication (12 cycles at high power, 15s ON / 90s OFF) with a Bioruptor (Diagenode). 4.4 μg of sheared nucleic acids were incubated with 10 μg of S9.6 antibody for immunoprecipitation overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Sonication was performed by splitting each sample to 2 Bioruptor tubes with sonication beads and using the Bioruptor Pico (Diagenode) for 18 cycles of 30 sec on and 30 sec off into an average size of approximately 200 bp ...
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bioRxiv - Genomics 2020Quote: ... high molecular weight DNA was diluted to a concentration of 25 ng/uL and fragmented to an average peak size of 25 kb using a Megaruptor-2 instrument (Diagenode). Small fragments <1 kb were removed and the DNA concentrated using bead purification (0.4 x volumes of Ampure-XP beads ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 95°C for 5 min and sonicated at 4°C for 2 cycles of 20 s at high intensity level using a BioRuptor (Diagenode). Samples were clarified by centrifugation at 16,000 x g for 15 min ...
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bioRxiv - Developmental Biology 2019Quote: ... and sonication for 2 cycles of 15s ON and 15s OFF on medium power at 4°C on a PicoBioruptor (Diagenode). After 10 minutes incubation at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... and sonicated for 2 cycles of 15s ON and 15s OFF on full power at 4°C on a Bioruptor (Diagenode). Samples were incubated for 1 hour on a rotating wheel at 4°C and centrifuged 10 minutes at 12,000 g and 4°C ...
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bioRxiv - Immunology 2021Quote: ... 2 µg of antibody was used per reaction. H3K9me3 (Lot. A2217P) and H3K4me3 (Lot No. A1052D) antibodies were from Diagenode. Immunoprecipitated and input DNA were extracted by phenol-chloroform extraction and used for qPCR with SsoAdvanced Universal SYBR Green supermix (Biorad) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using phenol/chloroform and 2 µg of purified 3C sample was sonicated using the Bioruptor Pico (Diagenode). Biotinylated DNA was captured using MyOne Streptavidin T1 magnetic beads (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... which were incubated overnight on a rotatory wheel at 4°C with the primary antibodies H4K20me1 (Diagenode; #C15410034; 2 μg), H3K9me3 (Diagenode ...
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bioRxiv - Cancer Biology 2022Quote: ... Shearing was performed in 1.5 ml Bioruptor® Pico Microtubes with Caps (Diagenode Cat# C30010016) with the following cell number ...
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bioRxiv - Molecular Biology 2022Quote: ... The liquid was distributed into 1.5 mL sonication tubes (Diagenode C30010016, 300 µL per tube) and sonicated for 12 min total (24 cycles of 30 s on/off ...
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bioRxiv - Cancer Biology 2023Quote: ... Shearing was performed in 1.5 ml Bioruptor® Pico Microtubes with Caps (Diagenode Cat# C30010016) with the following cell number ...
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bioRxiv - Genetics 2024Quote: ... Cell lysates were transferred to Diagenode 15 ml sonication tubes and sonicated (Bioruptor pico, Diagenode) 30 sec ON/ 45 sec OFF at 4°C for 60 cycles (6x10) ...
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bioRxiv - Biophysics 2024Quote: ... for 6 cycles (1 min on and 1 min off) on a Bioruptor Pico sonicator (Diagenode). ChIP lysates equivalent to 50 ug DNA were incubated in 1x IP buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... and H3K27me3 (Diagenode; #C15410195; 1:200). After washing ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-H3K36me3 (1:50, Diagenode C15200183), anti-H3K9me3 (1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-CTCF (1:400, Diagenode C15410210), anti-SMC1 (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... was added to the lysate and incubated at 37 °C for 10 min before sonication (2 × 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Microbiology 2022Quote: ... were added to the lysate and incubated at 37 °C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Biochemistry 2022Quote: ... heated to 95°C for 10 min shaking (1000 rpm) followed by 2 cycles à 20 s of sonication in BioRuptor (Diagenode). Aliquots containing 50 μg protein were transferred into a cartridge and cooled ...
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bioRxiv - Genomics 2022Quote: ... HMW-DNA (25 ng/μl) was separately sheared with 30 kb and 35 kb settings using a Megaruptor 2 instrument (Diagenode SA). However ...