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Citations for Diagenode :
151 - 200 of 329 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... Afterwards the lysate was sonicated for 15 cycles of 30 sec ON/OFF at 10 °C using a Bioruptor (Diagenode) and subsequently frozen at -80 °C ...
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bioRxiv - Cell Biology 2023Quote: ... beads were incubated for 30 min at RT with 5 µg of negative control antibody (C-, IgG from healthy rabbits, Diagenode). For immunoprecipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... and sonicated (6 cycles, high power for 30 s on and 30 s off at 4 °C, Bioruptor NextGen (Diagenode)) ...
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bioRxiv - Molecular Biology 2024Quote: ... for 5 min at 4°C and lysates were sonicated (1x 5 min, 30 sec on/off) with a Bioruptor (Diagenode) followed by digestion with 40U RNaseI (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... sonicated for 10 cycles with 30 ‘’ on and 30 ‘’ off at maximum power at 4 °C using a Bioruptor Pico sonication device (Diagenode). Afterwards ...
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bioRxiv - Cell Biology 2024Quote: ... by incubation for 5 min at 99°C followed by sonication (alternating 30 s on- and 30 s off-cycles at highest intensity output (Bioruptor, Diagenode)) ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were sonicated at 4°C for 10 min (30 sec ON, 30 sec OFF) at medium frequency (Bioruptor Diagenode). Then ...
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bioRxiv - Genomics 2021Quote: ... Labs 1 and 3 used Antibody 1 (Diagenode, Denville ...
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bioRxiv - Cell Biology 2020Quote: ChIP-seq experiments were carried out using the HighCell# ChIP-Protein G kit (Diagenode, Cat#: C01010063) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... The worm suspension containing fixed DNA-protein complexes was sheared using a Bioruptor (Diagenode, Denville, NJ) on the high setting for 20 cycles (30 seconds ON/30 seconds OFF) ...
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bioRxiv - Physiology 2020Quote: ... Cells were lysed and DNA-protein complexes were sheared using the Bioruptor Pico (Diagenode, ref B01060010) for 8 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... were used per ChIP and were bound to DiaMag protein G-coated magnetic beads (Diagenode, C03010021) overnight at 4°C with gentle rotation ...
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bioRxiv - Microbiology 2020Quote: ... was added to the lysate and incubated at 37 °C for 10 min before sonication (2 × 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Microbiology 2022Quote: ... were added to the lysate and incubated at 37 °C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Biochemistry 2022Quote: ... heated to 95°C for 10 min shaking (1000 rpm) followed by 2 cycles à 20 s of sonication in BioRuptor (Diagenode). Aliquots containing 50 μg protein were transferred into a cartridge and cooled ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein was extracted by incubating the islets with Laemmli buffer at 95°C for 5 mins and homogenized for another 5 mins in a sonication device (Diagenode, B01060001) at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... samples were sonicated at 4°C in 30 s on/off intervals for 15 cycles using a Bioruptor ® Plus sonication instrument (Diagenode) at high-intensity settings ...
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bioRxiv - Systems Biology 2021Quote: SARS-CoV-2 infected A549-ACE2 cell lysates were sonicated (10 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). Protein concentration was adjusted based on Pierce660 assay supplemented with ionic detergent compatibility reagent ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). HA or Streptactin beads were added to cleared lysates and samples were incubated for 3 hours at 4°C under constant rotation ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). Following normalization of protein concentrations of cleared lysates ...
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bioRxiv - Cell Biology 2021Quote: ... The homogenized samples were submitted to 10 cycles of 10 s at maximal intensity at 4⁰C in a sonicator bath (Bioruptor, Diagenode, USA). Then the homogenates were centrifuged at 10,000 g for 10 min at 4⁰C and the supernatants (NP40-soluble fraction ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: Membranes disruption was carried by sonication at 4 °C (2x10 min, 30 sec on, 30 sec off, high setting, Diagenode Bioruptor). Extracts were cleared by centrifugation (15,000 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
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bioRxiv - Systems Biology 2023Quote: ... 100 mM Tris-HCl pH 8.5) and boiled for 7 min at 95 °C before high-energy sonication to shear DNA (Diagenode Bioruptor pico). Protein concentrations were determined via a BCA assay (Thermo Fisher) ...
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bioRxiv - Biochemistry 2023Quote: Immunoprecipitation was performed using the IP star robot at 4°C (indirect 200 µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10 μg ...
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bioRxiv - Microbiology 2024Quote: ... were added to the lysate and incubated at 37°C for 10 min before sonication (2 x 8 bursts of 30 sec on ice using a Diagenode Bioruptor) to shear DNA fragments to a length of 300 to 500 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations of lysates were determined after 15 min of sonication (Bioruptor, Diagenode, cycles of 30 s) using the Pierce BCA Protein Assay (Thermo) ...
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bioRxiv - Genetics 2023Quote: ... H3K79me3 (1:1000; Diagenode, C15410068, or 1:1000; Abcam, ab2621), H3K27me3 (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... boiled at 95°C for 10 min and sonicated for 5 min using a Bioruptor® Plus sonication device (Diagenode, Liège, Belgium). Protein lysates were then run on a precast NuPAGE Bis-Tris 4–12% SDS–PAGE (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... 30 seconds off) for 7 cycles of sonication in a 4°C water bath using the Bioruptor Sonication System (Diagenode, Denville, NJ) at the high-intensity setting ...
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bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
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bioRxiv - Microbiology 2024Quote: ... and the samples were heated for 5 min at 95°C prior to sonication with a Bioruptor UCD-200 (Diagenode, Seraing, Belgium) device for ten cycles of 1 min pulse and 30 sec pause at high power ...
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bioRxiv - Molecular Biology 2021Quote: ... Commercial pA-Tn5 (protein A-Tn5) was purchased pre-loaded with sequence adapters from Diagenode (Denville, NJ, USA), and diluted to a working concentration of 1:50 with dilution buffer (40 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were sonicated for 10 minutes using a Bioruptor device together with protein extraction beads (Diagenode, Denville, NJ). Each lysate was diluted with 1080µl 50mM Tris pH7.4 ...
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bioRxiv - Neuroscience 2024Quote: ... we included a preclearing step by incubating the chromatin lysate with DiaMag Protein A-coated magnetic beads (Diagenode) at 4°C for 1.5 - 2 hours ...
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bioRxiv - Neuroscience 2019Quote: ... Fig.2A) using a closed system ultrasonic disruptor (10 cycles of 30s ON/30s OFF, 4°C; Bioruptor Pico, Diagenode sa, Liege, Belgium). ChIP assays were then conducted using antisera to CTCF (61311 ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
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bioRxiv - Immunology 2024Quote: ... We transferred cells into 1.5 ml sonication tubes (200 µL/tube) and sonicated 30 sec on/30 sec off for 6 cycles at 4 °C using Bioruptor® Pico (Diagenode, Denville, NJ). We centrifuged fragmented chromatin at 20,800 g for 10 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were taken up in 100 μL LYSE buffer and mixed with 50 mg of protein extraction beads (Diagenode). Samples were sonicated with 10-20 cycles at 30sec ON/OFF in a Diagenode Bioruptor Plus until complete tissue disruption was observed ...
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bioRxiv - Microbiology 2019Quote: ... incubated at 95°C for 10 min and further sonicated for 15 cycles à 30 s at high intensity level and 4°C using Bioruptor®Plus (Diagenode, Liege, Belgium). Protein concentrations were determined by measuring the tryptophan fluorescence at an emission wavelength of 350 nm using 295 nm for excitation with an Infinite® M1000 PRO microplate reader (Tecan ...
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bioRxiv - Cancer Biology 2023Quote: ... boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico, Diagenode; 4 cycles of 30s ON + 90s OFF). In both cases ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a maximum of 600 ng of DNA per accession was sheared at 4°C to a target average length of 400 bp using a Bioruptor® Pico sonication device (Diagenode, Denville, NJ, USA). Illumina libraries were prepared using a modified KAPA protocol with the KAPA LTP Library Preparation Kit (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... A modified protocol for on-beads digest (sonication for 10*30s in a Bioruptor sonicator in presence of 50 mg Protein Extraction beads, C20000021, Diagenode) was used for tissue samples followed by a 3.5h digestion step ...
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bioRxiv - Molecular Biology 2023Quote: ... boiled for 5 min at 95°C 1000rpm in 2% SDC buffer (2% SDC, 100 mM Tris-HCl pH=8.5) and sonicated (Diagenode Bioruptor, 15 * 30s at high intensity). Protein concentration was determined via BCA Protein Assay (Thermo ...
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bioRxiv - Genetics 2021Quote: ... 3 to 7 days later individual colonies were picked and expanded and western blot was used to confirm Cas9 protein expression (Diagenode, C15200203). Cas9 expression in a high expressing line was confirmed by immunohistochemistry (5).
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bioRxiv - Genomics 2021Quote: ... H3K27me3 (1:100, C15410069, Diagenode), and SOX9 (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... H3K9me3 (Diagenode; #C15410193; 1:1000) and H3K27me3 (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... H4K20me1 (Diagenode; #C15410034; 1:500), H3K9me3 (Diagenode ...
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bioRxiv - Developmental Biology 2023Quote: ... H3K4me3 (Diagenode #C15410003, 1 μg), H3K27me3 (Diagenode #C15410069 ...
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bioRxiv - Cell Biology 2023Quote: ... H3K4me3 (1:1000; Diagenode C15410003), H3S10ph clone MC463 (1:2000 ...