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Citations for Diagenode :
151 - 200 of 369 citations for 7 Fluoro 6 amino 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and subsequently disrupted by 2 × 20” high intensity sonication cycles at 4°C in a BioRuptor (Diagenode). Protein concentration was determined using the Micro BCA™ protein assay kit according to manufacturer’s instructions (Thermo Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were disrupted by sonication (two cycles à 20 s at 4 °C) in a BioRuptor (Diagenode), and extracts were cleared by centrifugation at 15,000 x g for 1 min ...
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bioRxiv - Genetics 2021Quote: ... incubated on ice for 10 min and subsequently sonicated at 4 °C with a Bioruptor sonicator (Diagenode) at high power for 5 cycles with 30 s ON and 30 s OFF ...
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bioRxiv - Genomics 2019Quote: Immunoprecipitations from 105 cells were performed using IP star robot at 4°C (indirect 200µl method, Diagenode) with an anti-BrdU antibody (10μg ...
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bioRxiv - Genomics 2022Quote: ... the nuclei were briefly sonicated for 5 seconds at 4°C in a Bioruptor Pico sonicator (Diagenode). Permeabilized nuclei were incubated for 10 minutes at 37°C in a thermoshaker ...
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bioRxiv - Microbiology 2023Quote: ... the different antibodies were previously incubated at 4°C with protein G-coated magnetic beads (DiaMag, Diagenode), protease inhibitor cocktail and 0.1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were sonicated for 4 min (20 seconds on, 40 seconds off) using a Bioruptor Pico (Diagenode). After sonication samples were cleared with 1% triton X-100 and pelleted (16000 x g for 5 min at 4 °C) ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 30 min at 4°C with rocking and then sonicated (3 x 30 sec interval, high setting in a Bioruptor water bath sonicator, Diagenode). Lysates were cleared by centrifugation at 13,000 g for 15 min at 4°C ...
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bioRxiv - Genetics 2020Quote: ... The top 100 µl sperm suspension was sonicated at the medium level for five times with 3 seconds each (Bioruptor UCD-200; Diagenode). An aliquot of 2 µl sperm HTF suspension was mixed immediately with 50 µl of 4% PVP (Sigma ...
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bioRxiv - Genomics 2021Quote: ... we obtained 5 μg of high molecular weight genomic DNA (> 50% of fragments ≥ 40 kb) and sheared fragments to ~10 kb using the Megaruptor 3 (B06010003; Diagenode), followed by DNA repair and ligation of PacBio adapters using the SMRTbell Template Prep Kit v1.0 (100-991-900) ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation at 95C for 5 min the sample was sonicated 3 times for 30 sec on/off with a Bioruptor (Diagenode) on high power ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... in the presence of protease inhibitors and subjected to 3 × 10 cycles (30 seconds on and 30 seconds off) of sonication (Bioruptor, Diagenode) to generate chromatin fragments of ∼300 bp in length ...
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bioRxiv - Molecular Biology 2021Quote: ... the nuclear lysate was sonicated for 3 cycles of 30 seconds ON / 30 seconds OFF using a Bioruptor Pico (Diagenode) to ensure solubilisation of chromatin and then spun 18,000g for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... the remaining pellet was resuspended in 50μl RIPA supplemented with 1x Protease Inhibitor Cocktail and sonicated for 3-5 min with Picoruptor® (Diagenode). 10μg of proteins were then separated through precast 4-12% NuPAGE® Bis-Tris gels (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were sonicated briefly (3 cycles, 12 s on, 12 s off) at a high setting in a Bioruptor (Diagenode) sonicator ...
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bioRxiv - Genomics 2023Quote: ... Additional samples HG02554 and HG02953 were prepared for sequencing in the same way but with the following modifications: isolated DNA was sheared using the Megaruptor 3 instrument (Diagenode) twice using settings 31 and 32 to achieve a peak size of ∼15-20 kbp ...
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bioRxiv - Genomics 2023Quote: 15 μg of DNA was cleaned with a 1X AMPure PB beads (Pacific Biosciences) cleanup and sheared to a target size of 14 kilobases (kb) using the Megaruptor 3 instrument (Diagenode) with the following settings ...
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bioRxiv - Molecular Biology 2023Quote: ... 2mM EDTA) and briefly sonicated to remove flagella (ON 5 sec – OFF 30 sec x 3 Cycles, bioruptor Pico, Diagenode). The samples were transferred in low retention tubes ...
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bioRxiv - Genomics 2024Quote: HMW DNA was sheared to 20 kb using Covaris G-Tubes (for shallow-depth sequencing with MinION) or using the Megaruptor 3 (Diagenode; for high-depth sequencing with PromethION) ...
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bioRxiv - Physiology 2023Quote: ... and 500-µl volume) in a water bath sonicator set at 4°C (Bioruptor 300. Diagenode, Denville, NJ). After centrifuging at 10,000g for 10 mins at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154; H3K27me1: Diagenode: C15410045-50) diluted in 1% BSA ...
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bioRxiv - Developmental Biology 2024Quote: ... The sonication of samples was then performed at 4°C using a Bioruptor® Plus sonication device (Diagenode) at high-intensity settings ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors) and sonicated using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). Sonicated lysates were diluted 2× in 50mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors and sonication using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). 200μg of proteins for each sample were diluted to 1 ml with CoIP Lysis Buffer and incubated with 30μl of anti-FLAG M2 Magnetic Beads (Sigma M8823 ...
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bioRxiv - Plant Biology 2022Quote: ... A HiFi library was then prepared according to the “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” manual with an initial DNA fragmentation by Megaruptor 3 (Diagenode, Belgium) and final library size binning into defined fractions by SageELF (Sage Science ...
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bioRxiv - Cancer Biology 2024Quote: ... buffer with added protease and phosphatase inhibitors and sonicated on high for 30 seconds off 30 seconds on for 3 minutes (Diagenode Bioruptor). The protein concentration was determined using the Micro BCA TM protein assay kit (Thermo Fisher) ...
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bioRxiv - Genetics 2021Quote: ... ONT libraries were prepared using the EXP-NBD103 and SQK-LSK109 kits according to the manufacturer’s instructions and using 4 µg of 40Kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Genomics 2020Quote: ... For single crosslinked cells nuclei were sonicated for 4 cycles of 30s on/30s off using a Picoruptor (Diagenode). Immunoprecipitation was carried out overnight at 4°C using 2 μg of RUNX1 antibody (ab23980 ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then incubated at 4°C overnight with or without 12.5 μg of anti-5hmC antibody (C15220001, Diagenode) in freshly prepared 1X Immunoprecipitation (IP ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Cell Biology 2023Quote: ... Chromatin was fragmented by sonication at 4°C using a Bioruptor 300 (Diagenode; 45 cycles, 30s on/30s off). Immunoprecipitation used 4 μg ChIP validated antibody against NF-κB p65 (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were sonicated for 5 cycles of 30 s on/off (2.5 min total sonication time) at 4°C in a Bioruptor Plus sonication system (Diagenode) and pelleted by centrifugation at 8000 RPM for 15 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... 40 mM 2-Chloroacetamide, 50 mM HEPES pH 8.5), boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). For the intracellular proteomes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with mixing for 30 min at 4°C before sonicating with a Bioruptor® Pico (Diagenode). Lysates were then spun for 10 min at 15,000 g ...
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bioRxiv - Plant Biology 2024Quote: ... Primary antibody incubation was performed overnight at 4°C with gentle shaking using anti-H3K9me2 (Diagenode Cat. No. C15410060), Anti-Histone H3 (Sigma-Aldrich Cat ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitation was done with 2 µg of chromatin and 4 µg of antibody against H3K9me3 (Diagenode, C15410193, lot A2217P). Sequencing libraries were prepared from 5 ng of immunoprecipitated DNA as previously described [19 ...
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bioRxiv - Developmental Biology 2021Quote: ... and subsequently sonicated for 3 cycles (30 seconds ON, 30 seconds OFF) on low settings using a Bioruptor® Plus (Diagenode, #B01020001). Samples were then centrifuged for 10 min at 10.000 g and supernatant was collected ...
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bioRxiv - Genetics 2023Quote: ... H3K79me3 (1:1000; Diagenode, C15410068, or 1:1000; Abcam, ab2621), H3K27me3 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... lysed in 1% SDS containing buffer supplemented with 1x protease and phosphatase inhibitors, and sonicated for 4 x 10 cycles (30 sec on, 30 sec off) in temperature-controlled Bioruptor 300 (Diagenode). Debris was removed by centrifuging at 14,000 rpm at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... was then added at a final concentration of 1 % for 15 min at RT and samples were sonicated over 20 min (10 × 30 s pulses) at 4°C to cleave genomic DNA (Bioruptor, Diagenode). Anti-MAZ antibody was from Bethyl Laboratories (A301-652A) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split into 150 μl aliquots in 0.5 ml Eppendorf tubes and sonicated at 4°C for 30 cycles (30 seconds on/off) using a Bioruptor (Diagenode). Aliquots of the same samples were pooled and spun down at 12,000xg for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitation was performed using IP star robot at 4°C (indirect 200µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10μg ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 95°C for 5 min and sonicated at 4°C for 2 cycles of 20 s at high intensity level using a BioRuptor (Diagenode). Samples were clarified by centrifugation at 16,000 x g for 15 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were sonicated at 4° C for 20 minutes using a waterbath sonicator cycling 30 seconds on and 30 seconds off (Bioruptor Pico, Diagenode). Sonicated lysates were cleared by centrifugation at 18,000 g for 10 minutes at 4° C ...
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bioRxiv - Molecular Biology 2020Quote: ... with minor modifications: Cells were resuspended in PBS at 4°C after flow cytometry sorting and incubated with ChIP Crosslink Gold (Diagenode #C01019027 ...
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bioRxiv - Plant Biology 2019Quote: ... For 5-mC immunolocalization slides were first incubated over night at 4°C with a monoclonal anti-5-mC-antibody (Diagenode C15200003 ...