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Citations for Diagenode :
151 - 200 of 239 citations for 7 ACETOXY 6 METHOXY 3 4 DIHYDROQUINAZODIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The DNA was sheared by sonication (4x 7 cycles, 30s on / 30s off, power high; Bioruptor, Diagenode) at 4°C and lysates cleared by centrifugation at 16,100 x g at 4°C for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of antibody per IP (Cas9 – Diagenode C15310258 ...
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bioRxiv - Genomics 2021Quote: ... Labs 1 and 3 used Antibody 1 (Diagenode, Denville ...
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bioRxiv - Genomics 2024Quote: ... DNA was then sheared with Megaruptor 3 (Diagenode) (45/200/30 ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... The eluates were diluted 1:50 with ChIP buffer from the One Day ChIP kit (Diagenode) and subsequently subjected to a second ChIP in accordance with the One Day ChIP kit manual but performing an overnight antibody incubation under rotation at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... After this time, samples were sonicated twice (7 cycles 30s on, 30s off) with a Bioruptor Plus sonicator (Diagenode). In between the two sonication cycles samples were incubated at 12°C with 0.8ul of Benzonase (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 µg of either IgG control antibody (Diagenode; C15410206) or of a specific antibody were applied ...
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bioRxiv - Cell Biology 2023Quote: ... sonicated 3 × 30 s (power: middle, Bioruptor Plus, Diagenode) and then centrifuged at 16000 g for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin samples were sonicated for 7 cycles (30 s ON, 30 sec OFF, at High output) in a Bioruptor (Diagenode). The samples were heated to 95°C for 5 min and stored at -20°C ...
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bioRxiv - Genomics 2020Quote: ... One µg per 100,000 cells of the desired antibody (H3K4me3 C15410003, H3K27ac C15410196, H3K27me3 C15410195, all from Diagenode) is added into each well ...
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bioRxiv - Molecular Biology 2023Quote: Chromatin-immunoprecipitation (ChIP) for ChIP-qPCR was performed according to the One-day ChIP kit protocol (Diagenode; C01010080). Beads were exchanged to Dynabeads™ Protein A (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Chromatin immunoprecipitation (ChIP) for ChIP-qPCR was performed according to the one-day ChIP kit protocol (Diagenode; C01010080). For each ChIP ...
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bioRxiv - Immunology 2019Quote: ... 3 µL protease inhibitor cocktail 1µg of H3K27ac antibody (Diagenode) and incubated overnight at 4 ºC with rotation ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was sheared with the Megaruptor 3 system (Diagenode) using 35-40 cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... sheared to 3-5kb in length with Megaruptor (Diagenode Diagnostics), and used to generate sequencing libraries for the Sequel II System ...
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bioRxiv - Genomics 2023Quote: ... Lysates were sonicated for 3 cycles using Bioruptor Pico (Diagenode) and dialyzed against RIPA buffer for 3 hours at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... sonicated a minimum of 3 x 30 seconds (Diagenode sonicator), were optionally reduced with 100 mM dithiothreitol ...
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bioRxiv - Genomics 2024Quote: ... HMW DNA was sheared with Megaruptor 3 (Diagenode, B06010003 & E07010003) using settings 28/31 and used to generate PacBio HiFi libraries via the SMRTbell Prep Kit 3.0 (PacBio ...
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bioRxiv - Genetics 2021Quote: ... 3 to 7 days later individual colonies were picked and expanded and western blot was used to confirm Cas9 protein expression (Diagenode, C15200203). Cas9 expression in a high expressing line was confirmed by immunohistochemistry (5).
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bioRxiv - Systems Biology 2023Quote: ... 100 mM Tris-HCl pH 8.5) and boiled for 7 min at 95 °C before high-energy sonication to shear DNA (Diagenode Bioruptor pico). Protein concentrations were determined via a BCA assay (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... One hundred fifty microliters of Hank’s buffered salt solution with 1x protease inhibitor cocktail (in True MicroChIP kit, Diagenode) was added ...
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bioRxiv - Molecular Biology 2021Quote: ... for 6 cycles (1 min on/1 min off) on a Bioruptor Pico sonicator (Diagenode). For each ChIP reaction 4 % HEK293T-derived human spike-in lysate was combined with mESC lysate and incubated in 1x IP buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sonicated in 6 x 30 second intervals using a Bioruptor 300 Machine (Diagenode), then centrifuged for 10 minutes at 10,000g at 4°C ...
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bioRxiv - Genomics 2022Quote: ... We sheared the genomic DNA with the Megaruptor® 3 (Diagenode) and purified them using AMPure PB magnetic beads (Pacific Biosciences ...
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bioRxiv - Genomics 2023Quote: ... cerevisiae DNA S288C were sheared using a Megaruptor 3 (Diagenode, NJ); aliquot 1 ...
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bioRxiv - Microbiology 2023Quote: High molecular weight gDNA was sheared with the Megaruptor 3 (Diagenode) to obtain 10- 15 kbp fragments ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 % Triton X-100) supplemented with 1 x HALT in a 15 ml Bioruptor tube containing one scoop of sonication beads (Diagenode) and sonicated in Bioruptor Pico (Diagenode ...
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bioRxiv - Biophysics 2024Quote: ... for 6 cycles (1 min on and 1 min off) on a Bioruptor Pico sonicator (Diagenode). ChIP lysates equivalent to 50 ug DNA were incubated in 1x IP buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... and sonicated using a Bioruptor (Diagenode, medium power, 3 x 15 sec). Protein concentration was measured using the Bradford assay (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The DNA was sheared twice with the Megaruptor® 3 from Diagenode with a velocity setting of 30 and 31 ...
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bioRxiv - Genomics 2024Quote: ... High molecular weight genomic DNA was sheared mechanically on Megaruptor 3 (Diagenode) to generate 30 Kb fragments ...
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bioRxiv - Genomics 2020Quote: ... The sheared chromatin derived from one million cells was subjected to magnetic immunoprecipitation and tagmentation using an SX-8G IP-STAR® Compact Automated System (Diagenode) with the anti-GATA6 antibody (D61E4 ...
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bioRxiv - Developmental Biology 2019Quote: ... Homogenates were sonicated for 6 cycles of 30 seconds ON/30 seconds OFF on a PicoBioruptor (Diagenode), centrifuged for 10 minutes at 16,000 g at 4°C ...
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bioRxiv - Genomics 2023Quote: ... and sonicated for 6 cycles of 30s ON/30s OFF on high power using the Bioruptor Plus (Diagenode). Insoluble material was removed by centrifugation at >16,000g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were incubated with blocking buffer (5% milk powder in TBST) for one hour at room temperature and afterwards incubated with primary antibody (anti-H3K27me3, Diagenode # C15410195, 1:1000) in blocking buffer overnight at 4 °C ...
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bioRxiv - Genomics 2019Quote: ... 3 µg of genomic DNA from each sample were fragmented using a Bioruptor (Diagenode), purified ...
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bioRxiv - Cell Biology 2019Quote: ... or Raji iBZLF1 cells induced for 6 h were lysed in RIPA buffer and sheared with the BioRuptor (Diagenode) four times (5 min ...
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bioRxiv - Plant Biology 2020Quote: ... samples were placed on ice for 10 min and sonicated for 6 cycles 15 sec “on” – 15 sec “off” using Bioruptor Plus (Diagenode). After that ...
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bioRxiv - Genomics 2022Quote: ... Cell lysis was facilitated by passing the lysates through 26G needle 10 times during the incubation on ice, and by 6 cycles of sonication (30 sec “on”/30 sec “off”, low input) on a Bioruptor Pico (Diagenode). Lysates were then treated with 40 U DNase I (Worthington ...
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bioRxiv - Genomics 2023Quote: DNA was sheared to an appropriate size range (10-20 kb) using Megaruptor 3 (Diagenode) followed by Ampure bead purification ...
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bioRxiv - Genomics 2023Quote: DNA was sheared to an appropriate size range (10-20 kb) using Megaruptor 3 (Diagenode) followed by SMRTbell cleanup beads ...
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bioRxiv - Molecular Biology 2019Quote: ... added (Figure 5B-5D) or 2x 300 µl was sonicated in 2x 1.5 ml bioruptor pico microtubes (Diagenode, Figure 6-9). When using the APLP protease inhibitors ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were first sonicated for 6 cycles of 30 sec on/30 sec off under low power setting with Bioruptor™ Twin (Diagenode), and then centrifuged at 17,000 × g for 10 min at 4 °C ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 50 ng/μl and sheared to 6 Kb fragments using Megaruptor® 2 (Diagenode, Seraing, Belgium). Shearing quality was estimated with TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: ... 12 μg of DNA was prepared for sequencing using Megaruptor 3 shearing (Diagenode, settings 19/31) and the Express Template Prep Kit v2 and SMRTbell Cleanup Kit v2 (PacBio) ...
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bioRxiv - Neuroscience 2024Quote: ... The lysates were then sonicated with 3 × 10 cycles (30 s on and off) (Bioruptor, Diagenode) to generate chromatin fragments of ∼500 bp in length ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2–5.5 µg of each sample was sheared using the Megaruptor 3 instrument (Diagenode, Liege, Belgium) at 18-20ng/µl and speed setting 31 ...
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bioRxiv - Genomics 2020Quote: ... The latter aliquot was processed for ChIP using 3 μg anti-H3K4me3 antibody (pAb-003-050, Diagenode) as indicated in the user manual ...
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bioRxiv - Genetics 2020Quote: ... 30 s ON/30 s OFF for 15 min × 3 times in Bioruptor Sonication System UCD200 (Diagenode) in 15 ml polystyrene conical tubes ...
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bioRxiv - Genomics 2020Quote: ... the DNA was sheared to between 15 kb and 23 kb using the Megaruptor® 3 (Diagenode). HiFi sequencing libraries were prepared28 using SMRTbell™ Express Template Prep Kit 2.0 and followed by immediate treatment with the Enzyme Clean Up Kit (PN ...