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Citations for Diagenode :
101 - 123 of 123 citations for n4 Benzoyl 5 methyldeoxycytidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... beads were incubated for 30 min at RT with 5 µg of negative control antibody (C-, IgG from healthy rabbits, Diagenode). For immunoprecipitation ...
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bioRxiv - Neuroscience 2024Quote: ... Cell extracts were then sonicated at 4°C for 5 cycles (30 seconds on / 30 seconds off) with the Bioruptor Pico (Diagenode), prior to proceeding with protein quantification using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Cortex nuclei were purified as above and collected by centrifugation (500 g; 5 min) for immediate use in ATAC Library preparation (Tagmentase–loaded; Diagenode). The nuclei pellets were suspended in 25 μL 2xTagmentation Buffer (Diagenode ...
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bioRxiv - Genomics 2024Quote: A starting amount of 4-5 ug HMW gDNA was sheared to a target size of 20-30 kbp using a Megaruptor 3 instrument (Diagenode). The sheared DNA underwent size selection using a Pippin HT instrument (Sage Science ...
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bioRxiv - Plant Biology 2024Quote: ... Twenty µl was saved as input and the remaining supernatant was then incubated at 4°C overnight with specific antibodies (5 µg of H3K27me3 Ab, Diagenode C15410069 or Millipore 07-449 ...
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bioRxiv - Molecular Biology 2024Quote: ... and then sonicated with series of 30 s ON / 30 s OFF for 5 min on a Bioruptor device (Diagenode) and centrifuged at 16,000 g for 15 min at 4 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Molecular Biology 2021Quote: ... A portion of sheared DNA (10%) was kept as input and the rest of the sheared DNA was immunoprecipitated with α-5-methylcytosine antibody (Diagenode), bound to magnetic beads ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). HA or Streptactin beads were added to cleared lysates and samples were incubated for 3 hours at 4°C under constant rotation ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). Following normalization of protein concentrations of cleared lysates ...
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bioRxiv - Cancer Biology 2021Quote: ... sodium deoxycholate and 0.5% (vol/vol) N-lauroylsarcosine) and the chromatin was sheared to 300 – 800 base pair (bp) fragments by Bioruptor (Diagenode, Belgium) 12 cycles of 30 second “ON” and 30 second “OFF” intervals ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF, pH 8.5) and sonicated for 5 min (30 sec on, 1 min off) with Bioruptor UCD-200TM (Diagenode, Liège, Belgium). The resulting cell lysates were centrifuged ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
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bioRxiv - Genomics 2023Quote: ... A portion of sheared DNA (10 %) was kept as input and the rest of the sheared DNA was immunoprecipitated with α-5-methylcytosine antibody (Diagenode), bound to magnetic beads ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1% Tween-20) and lysed by pulsed ultrasonication (5 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300). Cell lysates were subjected to tandem affinity purification as described above ...
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bioRxiv - Plant Biology 2024Quote: ... 65µg of genomic DNA was split into 5 aliquots and manually sheared with the Megaruptor 3 instrument (Diagenode, P/N B06010003), according to the Megaruptor 3 operations manual ...
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bioRxiv - Microbiology 2021Quote: ... boiled at 95°C for 10 min and sonicated for 5 min using a Bioruptor® Plus sonication device (Diagenode, Liège, Belgium). Protein lysates were then run on a precast NuPAGE Bis-Tris 4–12% SDS–PAGE (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and the samples were heated for 5 min at 95°C prior to sonication with a Bioruptor UCD-200 (Diagenode, Seraing, Belgium) device for ten cycles of 1 min pulse and 30 sec pause at high power ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were incubated with blocking buffer (5% milk powder in TBST) for one hour at room temperature and afterwards incubated with primary antibody (anti-H3K27me3, Diagenode # C15410195, 1:1000) in blocking buffer overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 30 s by 30 s at mild intensity and 5 times for differentiating myoblasts using a Bioruptor UCD-200 (Diagenode, Denville, NJ, USA). Then the samples were incubated with the anti-MTF1 antibody or IgG as a negative control[52] ...
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bioRxiv - Microbiology 2022Quote: ... resuspended in 1 ml PBS and sonicated at high intensity for 5 min in 30 s on 30 s off cycles using a Bioruptor (Diagenode, Denville, NJ, USA). Protein was determined in all samples by the Bradford method and a known mass of sample was mineralized in concentrated HNO3 trace metal grade (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mM TEAB and 1 x Halt protease inhibitor cocktail) and sonication (3 × 5 min cycles of 30s on/off; Diagenode Bioruptor sonication device). Extracted proteins protein were digested to peptides with in-solution digestion performed at the Turku Proteomics Facility ...
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bioRxiv - Neuroscience 2024Quote: ... The pellets were solubilized by resuspension in 6M Guanidine-HCl and sonication using a water-bath sonicator (Bioruptor, 5 min max output (Diagenode, Denville, NJ, USA)) ...