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Citations for Diagenode :
101 - 150 of 437 citations for Pregnanediol 3 Glucuronide PDG ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 xg ...
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bioRxiv - Molecular Biology 2019Quote: The soluble fraction was isolated by centrifugation at 2,000 rpm for 3 min then sonicated using a bioruptor (Diagenode) for 30 min in bursts of 30 s on/30 s off at high level in a 4°C water bath to produce sheared chromatin with a size range of 200-1,000 bp ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... by 30-min incubation on ice followed by sonicating for 3 min at medium power using a Bioruptor (Diagenode). Protein concentration was measured by Bradford assay (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... the extracted DNA was sheared to a target size of 18-20 kb using the Megaruptor 3 (Diagenode, B060100003). After confirmation of correct sizing ...
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bioRxiv - Genetics 2023Quote: ... 3) around 400 μl of the slurry were transferred to 0.6 ml tubes and sonicated in the Bioruptor (Diagenode) at + 4°C for 1 hour on the “high” setting with sonication intervals set to 45 seconds on/15 seconds off ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were sonicated (settings: 3 – 4 x 30 sec on /30 sec off, 4°C, power high; Bioruptor, Diagenode) and lysates were cleared by centrifugation at 16,000 x g at 4°C for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Genomics 2020Quote: ... 5 μg of purified DNA was sheared at 8 kb using the megaruptor1 system (Diagenode), followed by a DNA damage repair step on 2 μg of sample ...
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bioRxiv - Plant Biology 2021Quote: Methylated DNA immunoprecipitation (MedIP) assays were performed using anti 5-mC (Diagenode Mab-081-100) and anti-IgG (Abcam ab6702) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were sonicated (5× cycles of 30 s on and 30 s off; Bioruptor, Diagenode) and clarified by centrifugation (16,000 × g for 15 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... sonicated (5 cycles of 30 s ON, 30 s OFF, high power, Bioruptor Plus, Diagenode), spun for 10 min at 16,000 × gmax to remove any insoluble material ...
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bioRxiv - Genomics 2022Quote: ... 5 ug of genomic DNA was sheared to approximately 15-20 kb using Megaruptor3 (Diagenode). The sheared DNA was treated with DNA Prep to remove single-stranded ends ...
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bioRxiv - Cell Biology 2023Quote: ... nuclei were sonicated in ice water for 5×30 s (power: middle, Bioruptor Plus, Diagenode). Samples were centrifuged for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 30 min at 4°C with rocking and then sonicated (3 x 30 sec interval, high setting in a Bioruptor water bath sonicator, Diagenode). Lysates were cleared by centrifugation at 13,000 g for 15 min at 4°C ...
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bioRxiv - Genetics 2020Quote: ... The top 100 µl sperm suspension was sonicated at the medium level for five times with 3 seconds each (Bioruptor UCD-200; Diagenode). An aliquot of 2 µl sperm HTF suspension was mixed immediately with 50 µl of 4% PVP (Sigma ...
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bioRxiv - Genomics 2021Quote: ... we obtained 5 μg of high molecular weight genomic DNA (> 50% of fragments ≥ 40 kb) and sheared fragments to ~10 kb using the Megaruptor 3 (B06010003; Diagenode), followed by DNA repair and ligation of PacBio adapters using the SMRTbell Template Prep Kit v1.0 (100-991-900) ...
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bioRxiv - Cell Biology 2019Quote: ... in the presence of protease inhibitors and subjected to 3 × 10 cycles (30 seconds on and 30 seconds off) of sonication (Bioruptor, Diagenode) to generate chromatin fragments of ∼300 bp in length ...
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bioRxiv - Molecular Biology 2021Quote: ... the nuclear lysate was sonicated for 3 cycles of 30 seconds ON / 30 seconds OFF using a Bioruptor Pico (Diagenode) to ensure solubilisation of chromatin and then spun 18,000g for 10 minutes ...
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... Sonification was performed for 10 sec at level 1 using “constant” mode with a 3 mm sonification tip (Branson) or in a Bioruptor Pico (Diagenode) for 2 cycles 30 sec each ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were sonicated briefly (3 cycles, 12 s on, 12 s off) at a high setting in a Bioruptor (Diagenode) sonicator ...
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bioRxiv - Genomics 2023Quote: ... Additional samples HG02554 and HG02953 were prepared for sequencing in the same way but with the following modifications: isolated DNA was sheared using the Megaruptor 3 instrument (Diagenode) twice using settings 31 and 32 to achieve a peak size of ∼15-20 kbp ...
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bioRxiv - Genomics 2023Quote: 15 μg of DNA was cleaned with a 1X AMPure PB beads (Pacific Biosciences) cleanup and sheared to a target size of 14 kilobases (kb) using the Megaruptor 3 instrument (Diagenode) with the following settings ...
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bioRxiv - Genomics 2024Quote: HMW DNA was sheared to 20 kb using Covaris G-Tubes (for shallow-depth sequencing with MinION) or using the Megaruptor 3 (Diagenode; for high-depth sequencing with PromethION) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1μL SYBR green 10X (Diagenode kit), 1.3 μL of molecular biology grade water and 5μL of 2xPCR NEB master mix ...
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bioRxiv - Genetics 2020Quote: ... using the LowCell# ChIP kit (Diagenode). Illumina sequencing libraries were generated using the Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences) ...
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bioRxiv - Developmental Biology 2022Quote: ... and the iDeal ChIP-kit (Diagenode) for MED1 according to the manufacturer’s protocols with some modifications as described below ...
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bioRxiv - Developmental Biology 2023Quote: ... the iDeal ChIP-Seq kit (Diagenode) was used according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... using a Microplex v3 kit (Diagenode).
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bioRxiv - Genomics 2024Quote: ... the µChipmentation kit for histones (Diagenode) was used for chromatin immunoprecipitation and ChIP-Seq library preparation (Supplementary table 2).
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bioRxiv - Molecular Biology 2019Quote: ... lysates were sonicated for 5 min using a Bioruptor® Plus sonication device (Diagenode, Liège, Belgium). Protein concentration was assayed using the Micro bca protein assay kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... After sonication (15 sec on/ 30 sec off, 5 cycles, 4 C) using Bioruptor 300 (Diagenode) 5 µl were stained with DAPI to verify isolation of intact Macs ...
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bioRxiv - Molecular Biology 2021Quote: ... sonicated with a series of 30s ON / 30s OFF for 5 min on a Bioruptor (Diagenode), and centrifuged at 16,000 g for 5 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... pH 8.0) and sonicated for 5 min (30 sec ON/ 30 sec off) with Bioruptor (Diagenode). Total protein was measured with a Bradford assay (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were heated at 72 °C for 5 min and sonicated using a Bioruptor (Diagenode Inc) with fifteen 30 s on/off cycles at maximum intensity ...
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bioRxiv - Molecular Biology 2022Quote: ... After sonication for 5 cycles of 30 seconds ON/OFF at 4 °C (Bioruptor Pico, Diagenode), the mixture was centrifuged (13 000xg ...
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bioRxiv - Systems Biology 2023Quote: ... single-stranded DNA mixture was incubated with 5-mC antibody provided by MagMeDIP-seq Package (Diagenode), followed by 14-16 cycles of library amplification ...
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bioRxiv - Genomics 2022Quote: ... Proximity ligated chromatin pellet was sonicated and immunoprecipitated using 5 μg of H3K27ac antibodies (Diagenode, C15410196). Immunoprecipitated fragments were Biotin labeled (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... was used and 5 cycles sonicated then (Bioruptor® Plus and Bioruptor® Next Gen, Diagenode). Then supernatant was diluted to dilution buffer 2.5 times ...
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bioRxiv - Neuroscience 2020Quote: PFF transduction was conducted at 10 DIV by adding 2 ug/ml (96 well plate and coverslips) or 10 ug/ml (biochemistry) of PFFs diluted in PBS and sonicated with a BioRuptor (Diagenode) bath sonicator for 10 cycles of 30 seconds on ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors) and sonicated using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). Sonicated lysates were diluted 2× in 50mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% Triton-100 with protease inhibitors and sonication using a Bioruptor Pico (Diagenode, 3× cycles 30 sec on / 30 sec off). 200μg of proteins for each sample were diluted to 1 ml with CoIP Lysis Buffer and incubated with 30μl of anti-FLAG M2 Magnetic Beads (Sigma M8823 ...
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bioRxiv - Cancer Biology 2024Quote: ... buffer with added protease and phosphatase inhibitors and sonicated on high for 30 seconds off 30 seconds on for 3 minutes (Diagenode Bioruptor). The protein concentration was determined using the Micro BCA TM protein assay kit (Thermo Fisher) ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed using 75 µg of chromatin and 5 µg of Cas9 antibody (Diagenode #C15200229-100) or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913 ...
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bioRxiv - Cancer Biology 2019Quote: ... and subsequently lysed by boiling for 5 min at 95°Cand sonication (Bioruptor, model ACD-200, Diagenode) for 15 min at level 5 (30 sec ON ...
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bioRxiv - Developmental Biology 2021Quote: 5×105 fixed nuclei were sonicated to a 200-500bp length with the Bioruptor Pico sonicator (Diagenode). H3K27Ac ChIP was (Diagenode C15410174 ...
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bioRxiv - Genomics 2022Quote: ... the nuclei were briefly sonicated for 5 seconds at 4°C in a Bioruptor Pico sonicator (Diagenode). Permeabilized nuclei were incubated for 10 minutes at 37°C in a thermoshaker ...
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bioRxiv - Plant Biology 2022Quote: ... Cells were ruptured by 5 × 15 sec sonication in an ice bath in Bioruptor UCD-200 (Diagenode) at medium intensity with 2 min breaks on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... and then sonicated for 5 min at high setting with 30sON/30sOFF in a Bioruptor sonicator (Diagenode) and centrifuged for 5 min at 19000g at 4°C ...
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bioRxiv - Genetics 2024Quote: ... 5 μg of genomic DNA from each treatment condition was sheared using a bioruptor sonication device (Diagenode) in low mode for two cycles (30s on + 60s off ...
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bioRxiv - Molecular Biology 2021Quote: ... the Microplex Library Preparation kit V2 (Diagenode) was used for ChIP-Seq library construction following the manufacturer recommendations ...