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Citations for Diagenode :
101 - 150 of 365 citations for Cow Glucosidase 2 Subunit Beta PRKCSH ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... the immunoprecipitation (IP) and library preparation steps were performed following a modified µChIPmentation protocol (Diagenode, Supplementary table 2) using Diagenode antibodies for immunoprecipitation (30 ng of chromatin per IP ...
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bioRxiv - Cell Biology 2024Quote: ... Chromatin corresponding to 5 μg of DNA was incubated with 2 μg of anti-H3K4me3 (Diagenode; Cat#: C15410003) or anti-H3K9me3 (Diagenode ...
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bioRxiv - Neuroscience 2024Quote: ... Thawed samples were sonicated (2 cycles [10 s], 30 s between cycles, high setting; Bioruptor Next Gen, Diagenode) and heated (+90 °C for 7 min) ...
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bioRxiv - Neuroscience 2021Quote: ... using the MicroPlex Library Preparation Kit v2 (Diagenode Cat. C05010013). 50-bp single-end sequencing was performed on all libraries by Diagenode (Belgium ...
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bioRxiv - Molecular Biology 2021Quote: In the ChIPmentation Kit for Histones (Diagenode, Cat. No. C01011009) the suggested strategy is to sequence one sample immunoprecipitated with a control IgG and to use it for sequencing normalization instead of the classical input which cannot be treated exactly the same way as the immunoprecipitated samples ...
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bioRxiv - Neuroscience 2020Quote: ... RRBS libraries were prepared using the Premium RRBS Kit (Diagenode) and purified twice using Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... using iDeal ChIP-seq kit for Transcription Factors (Diagenode, C01010055). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared using a MicroPlex Library Preparation Kit (Diagenode) and sequenced on the NextSeq platform.
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bioRxiv - Cancer Biology 2023Quote: ChIP was performed using the Diagenode True Microchip kit (Diagenode), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ChIP was performed with True ChIP-seq Kit (Diagenode: C01010132) following manufacturers guidelines with the exception that bead bound nuclei were fixed with 36.5% formaldehyde for 1 min ...
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bioRxiv - Immunology 2023Quote: ... Libraries were prepared using MicroPlex Library Preparation Kit v3 (Diagenode) with 24 UDI for MicroPlex v3 (Diagenode) ...
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bioRxiv - Genomics 2023Quote: ... or the Diagenode MicroPlex Library Preparation kit v3 (Diagenode C05010001) following manufacturer’s instructions with few modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... following the iDeal ChIP-seq kit for Transcription Factors (Diagenode) protocol ...
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bioRxiv - Genomics 2024Quote: ... Shearing was performed with the Megaruptor shearing kit (E07010003, Diagenode) at speed 30 or 31 ...
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bioRxiv - Physiology 2024Quote: Library preparation was performed using the Premium RRBS kit (Diagenode). In brief ...
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bioRxiv - Developmental Biology 2024Quote: ... and an iDeal ChIP–seq Kit for Transcription Factors (Diagenode) as previously described20 (see Supplemental Methods) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and iDeal ChIP-seq Kit for Transcription Factors (Diagenode, #C01010055) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Cellular human and viral material in sputum deposited on glass slides was lysed in 50 μl 2% SDS dissolved in 50 mM Tris/HCl (pH 8.2) followed by sonication in a Bioruptor Pico (Diagenode). Proteins were digested using the SP3 protocol as described below.
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bioRxiv - Cell Biology 2021Quote: ... DNA was fragmented with 2 rounds of 30 cycles 30sec ON-30sec OFF of sonication with Bioruptor Plus (Diagenode) set on HIGH and purified with Qiagen MinElute kit (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitation was done with 2 µg of chromatin and 4 µg of antibody against H3K9me3 (Diagenode, C15410193, lot A2217P). Sequencing libraries were prepared from 5 ng of immunoprecipitated DNA as previously described [19 ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was sheared to a modal size of 10 kb using a Megaruptor 2 (Diagenode Diagnostics, Seraing (Ougrée), Belgium) ...
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bioRxiv - Molecular Biology 2024Quote: ... native ChIP was performed using an increasing amount of H3K4me3 antibodies (for the Abcam 0, 2, 4, 6, 10μl, for the Diagenode 0 ...
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bioRxiv - Cell Biology 2024Quote: ... After 2 × 5 cycles of sonication (30 s “ON”, 30 s “OFF”, High intensity) with a Bioruptor UCD200 (Diagenode), the chromatin solution was diluted 10-fold to a final volume of 3 mL with ChIP dilution buffer before IP ...
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bioRxiv - Immunology 2021Quote: ... Samples were split into 2 x 1.5 mL in 15 mL polypropylene tubes suitable for the Bioruptor® Pico (Diagenode). BioRuptor Sonicator settings ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed and sonicated in the presence of 0.2% SDS for 30 cycles (30 s on, 30 s off) using the high setting on a Bioruptor sonicator (Diagenode). Cell debris were pelleted by centrifugation and SDS in the lysate supernatants was diluted to 0.07% ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ChIPseq was performed as previously described (Bouderlique et al., 2019) using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) and the ThruPLEX DNA-seq (Rubicon Genomics ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were lysed for 15 min on ice with occasional inversion every 2 min and then sonicated for 24 cycles with 30s on and 30s off using Bioruptor (Diagenode). After sonication ...
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bioRxiv - Molecular Biology 2020Quote: ... nucleic acids gently extracted from unfixed NTERA-2 cells were sheared by sonication (12 cycles at high power, 15s ON / 90s OFF) with a Bioruptor (Diagenode). 4.4 μg of sheared nucleic acids were incubated with 10 μg of S9.6 antibody for immunoprecipitation overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Sonication was performed by splitting each sample to 2 Bioruptor tubes with sonication beads and using the Bioruptor Pico (Diagenode) for 18 cycles of 30 sec on and 30 sec off into an average size of approximately 200 bp ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was transferred to a sterile 2 ml microcentrifuge tube and sonicated with the Bioruptor UCD-200 sonicator (Diagenode) for 1 min on the low setting (160W at 20kHz ...
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bioRxiv - Neuroscience 2020Quote: ... the crosslinked cells were resuspended in 2 mL of lysis buffer and sonicated (in a cold room) by using a Bioruptor (Diagenode) with the following parameters ...
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bioRxiv - Genomics 2020Quote: ... high molecular weight DNA was diluted to a concentration of 25 ng/uL and fragmented to an average peak size of 25 kb using a Megaruptor-2 instrument (Diagenode). Small fragments <1 kb were removed and the DNA concentrated using bead purification (0.4 x volumes of Ampure-XP beads ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 95°C for 5 min and sonicated at 4°C for 2 cycles of 20 s at high intensity level using a BioRuptor (Diagenode). Samples were clarified by centrifugation at 16,000 x g for 15 min ...
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bioRxiv - Immunology 2021Quote: ... 2 µg of antibody was used per reaction. H3K9me3 (Lot. A2217P) and H3K4me3 (Lot No. A1052D) antibodies were from Diagenode. Immunoprecipitated and input DNA were extracted by phenol-chloroform extraction and used for qPCR with SsoAdvanced Universal SYBR Green supermix (Biorad) ...
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bioRxiv - Neuroscience 2023Quote: ... α-Synuclein PFFs were vortexed and diluted with DPBS to 2 mg/mL and sonicated in a cooled bath sonicator at 9°C (Diagenode Bioruptor® Pico ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed on ice for 10 minutes prior to sonication for either 20 cycles (replicate 1) or 40 cycles (replicate 2) of 30s on / 30s off with a Bioruptor UCD-200 (Diagenode) at maximum power ...
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bioRxiv - Cell Biology 2023Quote: ... which were incubated overnight on a rotatory wheel at 4°C with the primary antibodies H4K20me1 (Diagenode; #C15410034; 2 μg), H3K9me3 (Diagenode ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using phenol/chloroform and 2 µg of purified 3C sample was sonicated using the Bioruptor Pico (Diagenode). Biotinylated DNA was captured using MyOne Streptavidin T1 magnetic beads (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: Insoluble fractions were further sonicated in 1xPBS with 2% SDS at high intensity using a Bioruptor® Sonication Device (Diagenode) for SDS-PAGE.
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed in 1 ml of extraction buffer by three freeze‒thaw cycles in liquid nitrogen and by sonication (2 cycles, 30 sec high-power pulse) using a Bioruptor Plus (Diagenode). After centrifugation (20,000 x g ...
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bioRxiv - Neuroscience 2024Quote: ... α-Synuclein PFFs were vortexed and diluted with DPBS to 2 mg/mL and sonicated in a cooled bath sonicator at 9°C (Diagenode Bioruptor® Pico ...
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bioRxiv - Genomics 2020Quote: ChIPmentation was performed using a ChIPmentation for Transcription Factor kit (Diagenode) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Libraries were prepared using the MicroPlex v2 Library Preparation Kit (Diagenode) and amplification was monitored in real-time on a LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2021Quote: ... ChIP was performed using the True Micro ChiP kit (Diagenode C01010130) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... using the iDeal ChIP-seq kit for Histones (Diagenode Cat# C01010059).
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bioRxiv - Cell Biology 2021Quote: ... Libraries were constructed using the MicroPlex Library Preparation Kit v2 (Diagenode) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and samples were purified using the iPure v2 bead kit (Diagenode). Libraries were constructed using the MicroPlex Library Preparation Kit v2 (Diagenode ...
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bioRxiv - Genomics 2020Quote: ... The immunoprecipitated DNA was purified with the iPure kit (Diagenode, c03010014). Purified DNA was slot blotted onto a Hybond N+ membrane with the help of a dot-blot apparatus (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ChIP was performed with the iDeal ChIP-qPCR kit (Diagenode, #C01010180) according to the recommendation of the manufacturer ...