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Citations for Diagenode :
1251 - 1300 of 2740 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and chromosomal DNA was sheared to 100-500 bp fragments using the Bioruptor Pluswater bath sonication system (Diagenode, Liège, Belgium). Lysates were incubated with 40 μL Anti-FLAG M2 affinity gel (cat A2220 ...
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bioRxiv - Molecular Biology 2023Quote: ... The chromatin was sheared in a Bioruptor Plus (Diagenode) and then immunoprecipitated using Dynabeads coupled to the corresponding antibody ...
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bioRxiv - Microbiology 2023Quote: ... Chromatin was sonicated with Bioruptor (Diagenode) using 15mL probes for 12-15 cycles of 30 seconds ON 30 seconds OFF intervals at high intensity to achieve fragments in between 100 and 500bp ...
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bioRxiv - Molecular Biology 2023Quote: ... Sonication of the samples was carried out for ten minutes (30s ON – 30s OFF intervals) on Bioruptor® (Diagenode Diagnostics). The chromatins were checked with gel electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... carterae gDNA was first sonicated to 200-400 bp using the Bioruptor (Diagenode). A portion of sonicated DNA were retained as input sample ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: ... 50mg patient tissue RNA was extracted in an RNase free environment using RNA Extraction kit for Bioruptor Plus (Diagenode Cat# C20000010). The additional protocol listed for DNaseI treatment was followed for 10ug of extracted RNA per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... The chromatin was sonicated using a BioRuptor Pico sonicator (Diagenode; 5 cycles ...
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bioRxiv - Microbiology 2023Quote: ... The pellet corresponding to the nuclear fraction is dissolved in 300 µL of 1x SDS loading buffer with 100 mM DTT and sonicated with Bioruptor (Diagenode) 5 cycles of 30 seconds ON 30 seconds OFF intervals at high intensity ...
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bioRxiv - Cancer Biology 2023Quote: The ChIP-seq experiment has been conducted by Diagenode ChIP-seq/ChIP-qPCR Profiling service (Diagenode Cat# G02010000) ...
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bioRxiv - Cancer Biology 2023Quote: ... The chromatin was prepared by Diagenode ChIP-seq/ChIP-qPCR Profiling service (Diagenode Cat# G02010000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transposed DNA was then purified on Diapure columns (Diagenode, C03040001). The nuclei preparations were prepared using our standard protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... using the iDeal ChIP-seq kit for Histones (Diagenode Cat# C01010059). Chromatin was sheared using Bioruptor® Pico sonication device (Diagenode Cat# B01060001 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transposed DNA was then purified on Diapure columns (Diagenode, Transposed purified DNA was then pre-amplified for 5 PCR Cycles using NEBNext High-Fidelity PCR MasterMix (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... and sequencing libraries were prepared using a MicroPlex Library Preparation Kit (both Diagenode). The libraries were purified using AMPure® XP beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromatin was sheared using Bioruptor® Pico sonication device (Diagenode Cat# B01060001) combined with the Bioruptor® Water cooler for 8 cycles using a 30’’ [ON] 30’’ [OFF] settings ...
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bioRxiv - Cancer Biology 2023Quote: ... and sonicated by Bioruptor® (Diagenode) to yield DNA fragments around 500 base pairs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... High molecular weight DNA was extracted using a Circulomics Nanobind Tissue Big DNA Kit (NB-900-701-001) and sized to 15-25 kb using Bioruptor (Diagenode, Denville, NJ, USA). Sequencing libraries were constructed according to the manufacturers’ protocols and each sample was sequenced on one PacBio Sequel II 8M SMRT Cell for 30 hours in circular consensus sequencing mode to generate about 20 Gb of HiFi sequence data ...
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bioRxiv - Cell Biology 2023Quote: ... sonicated a minimum of 3 x 30 seconds (Diagenode sonicator), were optionally reduced with 100 mM dithiothreitol ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were sonicated on a Bioruptor Pico (Diagenode) for 3-8 cycles with 30 seconds ON/OFF ...
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bioRxiv - Cancer Biology 2023Quote: The library preparation has been conducted by Diagenode ChIP-seq/ChIP-qPCR Profiling service (Diagenode Cat# G02010000). Libraries were prepared using IP-Star® Compact Automated System (Diagenode Cat# B03000002 ...
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bioRxiv - Cancer Biology 2023Quote: ... The chromatin was prepared by Diagenode ChIP-seq/ChIP-qPCR Profiling service (Diagenode Cat# G02010000) using the iDeal ChIP-seq kit for Histones (Diagenode Cat# C01010059) ...
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bioRxiv - Cancer Biology 2023Quote: The ATAC-seq experiment was conducted by Diagenode ATAC-seq Profiling service (Diagenode Cat# G02060000) ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared using IP-Star® Compact Automated System (Diagenode Cat# B03000002) from input and ChIP’d DNA using MicroPlex Library Preparation Kit v3 (Diagenode Cat# C05010001) ...
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bioRxiv - Cancer Biology 2023Quote: ... from input and ChIP’d DNA using MicroPlex Library Preparation Kit v3 (Diagenode Cat# C05010001). Optimal library amplification was assessed by qPCR using KAPA SYBR® FAST (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Shearing was performed in 1.5 ml Bioruptor® Pico Microtubes with Caps (Diagenode Cat# C30010016) with the following cell number ...
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bioRxiv - Cancer Biology 2023Quote: The ChIP-seq experiment has been conducted by Diagenode ChIP-seq/ChIP-qPCR Profiling service (Diagenode Cat# G02010000). The chromatin was prepared by Diagenode ChIP-seq/ChIP-qPCR Profiling service (Diagenode Cat# G02010000 ...
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bioRxiv - Cancer Biology 2023Quote: ... ChIP was performed using IP-Star® Compact Automated System (Diagenode Cat# B03000002) following the protocol of the aforementioned kit ...
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bioRxiv - Cancer Biology 2023Quote: Cells were cross-linked with ChIP Cross-link Gold (Diagenode, # C01019027). After incubation at RT for 30min ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then sonicated (Bioruptor plus, Diagenode, 10 x 30s/30s ON/OFF cycles) and heated up to 37°C for 2 h while shaking (800 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... samples were thawed and sonicated (Bioruptor® Plus, Diagenode) for 10 cycles (30 s ON/60 s OFF ...
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bioRxiv - Cell Biology 2023Quote: ... sonicated 3 × 30 s (power: middle, Bioruptor Plus, Diagenode) and then centrifuged at 16000 g for 15 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... nuclei were sonicated in ice water for 5×30 s (power: middle, Bioruptor Plus, Diagenode). Samples were centrifuged for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were sonicated at 4°C in Bioraptor Pico (Diagenode) for 10 cycles ...
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bioRxiv - Cancer Biology 2023Quote: ... and sonicated (Bioruptor Pico, Diagenode, USA). Protein concentration was measured using the BCA assay (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... beads were incubated for 30 min at RT with 5 µg of negative control antibody (C-, IgG from healthy rabbits, Diagenode). For immunoprecipitation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Several libraries were constructed using the Ligation Sequencing Kit (Nanopore LSK109) using DNA sheared to different size using a megaRuptor (Diagenode) to optimise yield and contiguity ...
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bioRxiv - Genetics 2023Quote: ... and H3K9me3 antibody (C15410193, Diagenode)21 ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were rabbit anti-Cas9 (Diagenode C15310258, 1:100) and rabbit anti-HA (Cell Signaling 3724S ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then sonicated (Bioruptor Twin, Diagenode). Protein concentration of the cell lysis was assessed with BCA protein assay (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... and the air-dried pellets were resuspended in 500 μl 8 M urea and sonicated in a bioruptor (Diagenode) with high amplitude for 10 min with cycles of 30 seconds on and 30 seconds off.
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bioRxiv - Biochemistry 2023Quote: ... After glass-bead beating, chromatin was sonicated for 24 cycles (high output, 30 s-on, 30 s-off) using the Bioruptor Plus (Diagenode, Belgium) to produce fragments of ∼ 250 bp ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were taken up in 100 μL LYSE buffer and mixed with 50 mg of protein extraction beads (Diagenode). Samples were sonicated with 10-20 cycles at 30sec ON/OFF in a Diagenode Bioruptor Plus until complete tissue disruption was observed ...
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bioRxiv - Plant Biology 2023Quote: ... and sonicated four times (BioruptorTM, Diagenode, 30s on/1min off on High). They were quantified at 205 nm using the Nanodrop 2000 according to the manufacturer’s instruction (Thermoscientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromatin was sonicated in Shearing Buffer (0.1% SDS, 1mM EDTA and 10mM Tris pH 7.5) using Bioruptor sonicator (Diagenode; high power setting) to obtain fragments of about 500-800 bp ...
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bioRxiv - Cancer Biology 2023Quote: ... were lysed in buffer (50 mM Tris-HCl, pH 7.8) containing 1% SDS and 0.1 M dithiothreitol and sonicated (Diagenode Bioruptor Plus). Number of sonication cycles was established based on a visual sample clarity assessment ...
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bioRxiv - Cancer Biology 2023Quote: ... sonicated using Bioruptor Plus (Diagenode), and further processed according to standard phenol-chloroform RNA isolation protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was then incubated at 37 °C for 120 hours with constant agitation at 200 rpm and the product sonicated 10 min at 10 °C (0.5 min on/0.5 min off) using a Biorupter Pico (Diagenode). Aggregates were flash-frozen with liquid nitrogen in single-use aliquots for storage at -80 °C.
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bioRxiv - Cell Biology 2023Quote: ... a total of 10 ng of dsDNA was purified using the MicroPlex Library Preparation kit v3 (C05010001, Diagenode s.a., Seraing, Belgium), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and Bioruptor (Diagenode) was used to shear chromatin ...
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bioRxiv - Genetics 2023Quote: ... H3K79me1 (1:1000; Diagenode, C15410082), H3K79me2 (1:1000 ...