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Citations for Diagenode :
51 - 100 of 252 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... and sonicated at 4°C using a Bioruptor Pico sonication device (Diagenode) for 10 cycles (30s ON/30s OFF ...
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bioRxiv - Neuroscience 2023Quote: ... Tau was sonicated in a cooled bath sonicator at 9°C (Diagenode Bioruptor® ...
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bioRxiv - Biochemistry 2024Quote: ... The suspension was sonicated at 4°C with a Bioruptor (Diagenode, #B01020001) using conditions optimized to generate 200–500 bp chromatin fragments ...
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bioRxiv - Genomics 2024Quote: ... 10 μg of HMW DNA were sheared with either Megaruptor 3 (Diagenode; #B06010003) or a needle (FINEJECT ® 26Gx1” 0.45x25mm ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM NaCl) and sonicated for 30 minutes at 4°C (Diagenode Bioruptor). Anti-di-glycine peptide beads (Cell Signalling #5562 ...
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bioRxiv - Cell Biology 2022Quote: ... Chromatin was incubated overnight at 4°C using 0.25µg of H3K9me3 (C15410193-Diagenode) per IP ...
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bioRxiv - Plant Biology 2021Quote: ... Sonication of chromatin was done in 4°C water sonicator (Diagenode Bioruptor Plus). Sonicated samples were first precleared with Protein A Agarose beads (Millipore #16-157 ...
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bioRxiv - Synthetic Biology 2021Quote: ... with following antibodies at 4 °C on a rotating wheel: H3K27me3 (Diagenode, C15410195), Ring1B (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... 570 µM PMSF) for 10 min at 4 °C with sonication (Bioruptor, Diagenode, 10 min on ice ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154 ...
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bioRxiv - Genomics 2024Quote: ... Approximately 10 kb sheared DNA by the Megaruptor 3 system (Diagenode, Belgium; Cat. #B06010003) was used for removal of single-strand overhangs at 37°C for 15 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... The resuspended chromatin was sonicated in a 4°C water sonicator (Diagenode Bioruptor Plus). Serum containing anti-E2Fa antibodies was obtained from Prof ...
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bioRxiv - Biochemistry 2020Quote: ... samples were sonicated for 15 minutes (37°C) using a Bioruptor plus ultrasonicator (Diagenode). The second digestion step was done using fresh LysC (1:50 enzyme to protein ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref ...
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bioRxiv - Microbiology 2024Quote: ... before and after sonication at 4°C for 15min in a Bioruptor® (Diagenode), to determine Chain (ChFU ...
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bioRxiv - Synthetic Biology 2023Quote: ... heated at 95°C for 10 min and sonicated with the Bioruptor sonicator (Diagenode) for 15 cycles (30 s ON ...
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bioRxiv - Genomics 2023Quote: ... in the high mode at 4°C in a Bioruptor water bath sonicator (Diagenode), to obtain fragments of 500 to 1000 bp in size ...
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bioRxiv - Biochemistry 2024Quote: ... Chromatin was sheared at 4°C to 0.4-0.6 kb by sonification (Bioruptor+, Diagenode) for 12 cycles 30 sec ON 30 sec OFF on low power settings ...
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bioRxiv - Genomics 2023Quote: DNA was sheared to an appropriate size range (10-20 kb) using Megaruptor 3 (Diagenode) followed by Ampure bead purification ...
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bioRxiv - Genomics 2023Quote: DNA was sheared to an appropriate size range (10-20 kb) using Megaruptor 3 (Diagenode) followed by SMRTbell cleanup beads ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was sheared with the Megaruptor 3 system (Diagenode LLC, Denville, New Jersey, USA) using 35 cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... were washed 3 times with NETT buffer and mixed with α-Ty1 (Diagenode, Liege, Belgium), followed by incubated for 2.5 hours in this buffer ...
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bioRxiv - Genomics 2024Quote: ... the HMW DNA was sheared to approximately 18 kb fragments with a Megaruptor 3 (Diagenode), using 1 cycle 31x speed and 1 cycle at 32x speed ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was fragmented into 15-20 kb fragments using Megaruptor 3 (Diagenode, Denville, NJ, USA), and the final library was size-selected using BluePippin (Sage Science ...
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bioRxiv - Genomics 2024Quote: DNA samples were homogenized using a Diagenode Megaruptor with the 3 DNAFluid+ Kit (E07020001, Diagenode) under the following conditions ...
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bioRxiv - Plant Biology 2024Quote: ... The solution was sonicated to fragment chromatin into 1-3 kb using a BioRuptor (Diagenode) under high level intensity (30 sec ON and 50 sec OFF ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were denatured at 95°C and DNA was sonicated using a Bioruptor 300 (Diagenode) on the high setting for 8 cycles 30 sec ON / 30 sec OFF ...
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bioRxiv - Molecular Biology 2021Quote: ... with following appropriate antibodies at 4 °C o/n a rotating wheel: H3K27me3 (Diagenode, C15410195), RING1B (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 s on / 30 s off) at 4°C using a water bath-sonicator (Diagenode). Chromatin fragments were reverse cross-linked overnight at 65°C ...
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bioRxiv - Genomics 2023Quote: ... 6μL DNA extract were incubated (55°C) with 5μL of 2X Tagmentation Buffer (Diagenode, C01019043) and 1μL of Loaded Tn5 Tagmentase (Diagenode ...
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bioRxiv - Immunology 2024Quote: ... Samples were boiled for 10 min at 95 °C and sonicated in a Bioruptor (Diagenode) for 10 cycles of 30 sec on/30 sec off ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were kept on ice for one hour before sonication at 4°C (Bioruptor, Diagenode) to an average DNA length of 200–500 bp by 15 pulses of 10 sec on and 10 sec off at high intensity ...
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bioRxiv - Neuroscience 2024Quote: ... The lysates were then sonicated with 3 × 10 cycles (30 s on and off) (Bioruptor, Diagenode) to generate chromatin fragments of ∼500 bp in length ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2–5.5 µg of each sample was sheared using the Megaruptor 3 instrument (Diagenode, Liege, Belgium) at 18-20ng/µl and speed setting 31 ...
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bioRxiv - Genomics 2023Quote: ... 12 μg of DNA was prepared for sequencing using Megaruptor 3 shearing (Diagenode, settings 19/31) and the Express Template Prep Kit v2 and SMRTbell Cleanup Kit v2 (PacBio) ...
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bioRxiv - Molecular Biology 2021Quote: ... shear DNA and enhance cell disruption by using a water-bath (4°C) sonicator Bioruptor (Diagenode) at High Power for 40 min (sonication cycle ...
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bioRxiv - Genomics 2021Quote: ... After sonication (15 sec on/ 30 sec off, 5 cycles, 4 C) using Bioruptor 300 (Diagenode) 5 µl were stained with DAPI to verify isolation of intact Macs ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were heated at 72 °C for 5 min and sonicated using a Bioruptor (Diagenode Inc) with fifteen 30 s on/off cycles at maximum intensity ...
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bioRxiv - Genomics 2022Quote: ... Cell lysates were then sonicated 20 times at 4°C (Bioruptor Plus, Diagenode, 30/30 cycles) and centrifuged at 14000 rpm for 1 minute at 4°C to remove cellular debris ...
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: ... and subsequently lysed by 5min boiling at 95°C and sonication (Bioruptor, model UCD-200, Diagenode) for 15min ...
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bioRxiv - Molecular Biology 2022Quote: ... After sonication for 5 cycles of 30 seconds ON/OFF at 4 °C (Bioruptor Pico, Diagenode), the mixture was centrifuged (13 000xg ...
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bioRxiv - Physiology 2023Quote: ... 500µl volume) in a water bath sonicator set at 4°C (Bioruptor 300; Diagenode, Denville, NJ). After centrifuging at 10000g for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated chromatin was fragmented by sonication at 4°C for 10 minutes using Bioruptor (Diagenode). Run-on reactions were performed at 37°C for 5 minutes using biotinylated nucleotides (5 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated 10 minutes at 4°C and then sheared using a Bioruptor (Diagenode, Belgium) for 4 times at 5 cycles and followed by 2 cycles at 30″ ON/OFF high power ...
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bioRxiv - Cell Biology 2024Quote: ... the reverse crosslinking (5 h at 65°C) and elution were performed using IPure kit (Diagenode). The final elution was performed in 60 μL ...
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bioRxiv - Genomics 2020Quote: ... The latter aliquot was processed for ChIP using 3 μg anti-H3K4me3 antibody (pAb-003-050, Diagenode) as indicated in the user manual ...
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bioRxiv - Genetics 2020Quote: ... 30 s ON/30 s OFF for 15 min × 3 times in Bioruptor Sonication System UCD200 (Diagenode) in 15 ml polystyrene conical tubes ...
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bioRxiv - Genomics 2020Quote: ... the DNA was sheared to between 15 kb and 23 kb using the Megaruptor® 3 (Diagenode). HiFi sequencing libraries were prepared28 using SMRTbell™ Express Template Prep Kit 2.0 and followed by immediate treatment with the Enzyme Clean Up Kit (PN ...
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bioRxiv - Molecular Biology 2021Quote: ... then the sample was sonicated at a high intensity (3 × 5 min) using a Bioruptor sonicator (Diagenode). To obtain organoid-MPF cocultures ...
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bioRxiv - Genomics 2024Quote: ... HMW gDNA was sheared to 15-18 Kb using Diagenode’s Megaruptor 3 system (Diagenode, Belgium; cat. B06010003). The sheared gDNA was concentrated using 1x of SMRTbell cleanup beads for the repair and a-tailing incubation at 37°C for 30 minutes and 65°C for 5 minutes ...