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Citations for Diagenode :
51 - 100 of 432 citations for Mouse WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... in presence of 50 mg Protein Extraction beads (C20000021, Diagenode); samples were trypsin digested for 2 h 30 min ...
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bioRxiv - Bioengineering 2024Quote: Unloaded Tn5 transposase proteins were acquired from Diagenode (#C01070010-10). Tn5 transposase was then loaded with oligos containing mosaic ends incorporating 13 base-pair unique molecular identifiers (UMIs ...
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bioRxiv - Genomics 2020Quote: ... genomic DNA was sheared using the Megaruptor 2 (Diagenode) with a long hydropore and a 20 kb shearing protocol ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... and mouse anti-ERalpha (1:200; # C15100066, Diagenode, Liège, Belgium); negative controls were proceeded without one primary antibody or both ...
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bioRxiv - Genomics 2021Quote: ... Mouse anti-Cas9 (Encor MCA-3F9 and Diagenode C15200229-100) antibodies were used ...
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bioRxiv - Systems Biology 2023Quote: ... ∼400 ng DNA-containing chromatin was incubated with 1 µg antibody (H3K9/14ac (Diagenode, C15410005); H3K9me2 (in house ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg DNA was sonicated in a Bioruptor Plus (Diagenode). DamID adaptors were removed by AlwI digestion ...
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bioRxiv - Developmental Biology 2019Quote: ... subtracted to 2 cycles of sonication on a bioruptor (Diagenode) with 30 sec “ON”/“OFF” at low setting and the remaining cell debris was removed by centrifugation at 21,000 g for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: Antibodies used in this study were mouse anti-H3K27ac (Diagenode, C15200184) and rabbit anti-H3K4me3 (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were resuspended in RIPA Buffer containing complete protease inhibitors (EDTA-free) and sonicated (Bioruptor, Diagenode). Cellular debris was pelleted at 10 000 rpm for 10 min at 4°C and protein concentration determined ...
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bioRxiv - Molecular Biology 2019Quote: ... 1.2 μg total protein was analysed with Cas9 (Diagenode C15310258, 1:400) and GAPDH (EMD MAB374 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by immunoprecipation of immunocomplexes with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 1 hr at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Sheared chromatin was thereafter immunoprecipitated with Protein A-coated magnetic beads (Diagenode) preincubated with 4.5 μg/IP MED1 antibody (Bethyl A300-793A ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody-bound chromatin was then coupled to protein A beads (Diagenode, C03020002) for three hours at 4° C ...
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bioRxiv - Molecular Biology 2023Quote: ... boiled for 5 min at 95°C 1000rpm in 2% SDC buffer (2% SDC, 100 mM Tris-HCl pH=8.5) and sonicated (Diagenode Bioruptor, 15 * 30s at high intensity). Protein concentration was determined via BCA Protein Assay (Thermo ...
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bioRxiv - Developmental Biology 2021Quote: ... 3µm-sections were immunostained using an antibody anti-histone H3 containing the trimethylated lysine 27 (H3K27me3) (Diagenode). Heat induced antigen retrieval was done using CC1 basic buffer (Ventana) ...
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bioRxiv - Molecular Biology 2023Quote: MeDIP-Seq libraries for Ion Torrent semiconductor sequencing were performed on the Ion Torrent PGM using a modified protocol from Ion Plus Fragment Library kit (Catalog # 4471252) combined with a 5-hydroxymethylcytosine (5hmC Kit, Catalog # AF-110–0016) immunoprecipitation kit (Diagenode) according to a previously optimized protocol (Guerrero-Bosagna & Jensen ...
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bioRxiv - Genetics 2023Quote: ... pH 8.1) and 2.5 µg of mouse antibodies to H3K9me2 (Diagenode C15410060) and H3K9me3 (Diagenode C15410193) ...
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bioRxiv - Genomics 2023Quote: ... The samples were fragmented to 10 kb using Megaruptor 2 (Diagenode). For each fragmented sample ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of antibodies ChIP-grade (H3K4me2, Abcam ab32356; H3K4me3, Diagenode C15410003 ...
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bioRxiv - Immunology 2022Quote: ... iPure kit v2 (Diagenode) was used ...
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bioRxiv - Genomics 2019Quote: ... MethylCap kit (Diagenode, C02020010) was employed to obtain the methylated DNA ...
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bioRxiv - Immunology 2021Quote: ... iPure kit v2 (Diagenode) was used ...
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bioRxiv - Molecular Biology 2020Quote: ... The Low cell# ChIPkit protein A × 48 was from Diagenode (Denville, NJ, USA). TaqMan probes for mRNA expression and Chemiluminescence western blot detection reagent were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were applied: H3K4me1 (1:100, C15410037, Diagenode), H3K27ac (1:100 ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was purified according to the IPure kit v2 Kit manual (Diagenode, C03010015). The amount of immunoprecipitated DNA was quantified by qPCR ...
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bioRxiv - Genomics 2023Quote: ... following the manufacturer’s protocol with a mouse antibody against 5hmC (Diagenode, C15200200-50) or 5mC (Diagenode ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was sheared to 20 kb fragments using Megaruptor 2 (Diagenode) and size selected using the BluePippin system (Sage Science ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was sheared to 8 kb fragments using Megaruptor 2 (Diagenode). Standard SMRTbell libraries were prepared using Template Preparation Kit 1.0 (Pacific Biosciences ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... and 2.5 μg anti-enhancer of zeste homolog 2 (EZH2) (Diagenode, C15200180). Antibody-protein complexes were immunoprecipitated with protein A/G Dynabeads for 3 hours at 4°C with rotation ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 ug/ul of lysates were sonicated with a Bioruptor Plus (Diagenode) for 20 cycles (30 seconds ON and 30 seconds OFF) ...
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bioRxiv - Molecular Biology 2019Quote: ... The immune complexes were purified with DiaMag Protein G coated magnetic beads (C03010021, Diagenode). De-crosslinked DNA was eluted for qPCR to assess changes in Sox2 recruitment to specific areas of interest following 7SK knock down ...
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bioRxiv - Immunology 2020Quote: 1.5 ml Bioruptor Microtubes were filled with 250 mg of Protein Extraction Beads (Diagenode) and filled with RIPA buffer (supplemented with protease and phosphatase inhibitors) ...
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bioRxiv - Molecular Biology 2024Quote: ... The sheared chromatin was immunoprecipitated using the DiaMag protein A-coated magnetic beads (Diagenode) and 2 µg of anti-HvHsfA6a antibodies ...
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bioRxiv - Genomics 2019Quote: ... Auto hMeDIP kit (Diagenode C02010033) was used according to manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... using LowCell# ChIP kit (Diagenode), with 1μg of antibody against ARNTL (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... or hMeDIP kit (Diagenode, C02010031). Quantification of precipitated DNA was determined using real-time PCR and the delta Ct method for relative quantitation (ABI 7900HT Fast Real-Time PCR System).
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bioRxiv - Molecular Biology 2019Quote: ... The pellet was washed with 1 ml lysis buffer and gently resuspended in 300 µl lysis buffer containing 0.2% SDS and sheared by sonication with Bioruptor (Diagenode) for 30 minutes (30s On ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested in lysis buffer containing 0.1% Rapigest and sonicated (10 cycles of 30 seconds on, 30 seconds off in Bioruper, Diagenode). Approximately 30 μg of protein was resolved on a 4-20% Miniprotean TGX gel ...
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bioRxiv - Genetics 2020Quote: ... Mouse chromatin was ChIPped using the following amounts of antibody: 1µg H3K4me3 (Diagenode C1540003), 0.2µg H3K4me1 (Diagenode C1540194) ...
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bioRxiv - Genetics 2022Quote: ... The antibodies used were mouse anti-RNA Pol II (Diagenode C15100055-100 RRID: AB_2750842) and goat anti-KLF1 (Origene TA305808) ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was fragmented to 20 kb using Megaruptor 2 (Diagenode, Liege, Belgium) and size selected with a 10 kb cut-off using the Blue Pippin system (Sage Science ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was sheared to 20 kb fragments using the Megaruptor 2 (Diagenode) and size selected with a 10 kb cut-off using the BluePippin system (Sage Science) ...
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bioRxiv - Genomics 2021Quote: ... Binding was stopped by adding 57 µL 2 M glycine (Diagenode, cat. C01011000) and incubating the sample for 5 min at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was sheared to 20-kb fragments using Megaruptor 2 (Diagenode, Belgium) and then bead-size selected with AMPure PB beads (Pacific Biosciences ...
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bioRxiv - Zoology 2022Quote: ... 2 μg genomic DNA was sonicated using a Picoruptor (Diagenode s.a., Liège, Belgium) to produce 300–500-bp fragments ...
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bioRxiv - Plant Biology 2022Quote: HMW DNA was sheared using Megaruptor 2 system (Diagenode, Liège Science Park, Belgium) to obtain a 40 Kb average size ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μl of precleared chromatin was added to 98 μl buffer iE1 (Diagenode). To samples and inputs ...
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bioRxiv - Genomics 2022Quote: ... Cells were lysed and DNA-protein complexes were sheared using the Bioruptor Pico (Diagenode, #B01060010) for 8 minutes ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease and phosphatase inhibitor cocktails and sonicated for 1 min with the Bioruptor sonication device (Diagenode). Cell lysates were centrifuged at 13,000 × g for 10 min at 4 °C ...