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Citations for Diagenode :
51 - 100 of 164 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Samples were heated at 72 °C for 5 min and sonicated using a Bioruptor (Diagenode Inc) with fifteen 30 s on/off cycles at maximum intensity ...
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bioRxiv - Genomics 2022Quote: ... Cell lysates were then sonicated 20 times at 4°C (Bioruptor Plus, Diagenode, 30/30 cycles) and centrifuged at 14000 rpm for 1 minute at 4°C to remove cellular debris ...
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: ... and subsequently lysed by 5min boiling at 95°C and sonication (Bioruptor, model UCD-200, Diagenode) for 15min ...
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bioRxiv - Molecular Biology 2022Quote: ... After sonication for 5 cycles of 30 seconds ON/OFF at 4 °C (Bioruptor Pico, Diagenode), the mixture was centrifuged (13 000xg ...
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bioRxiv - Physiology 2023Quote: ... 500µl volume) in a water bath sonicator set at 4°C (Bioruptor 300; Diagenode, Denville, NJ). After centrifuging at 10000g for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated chromatin was fragmented by sonication at 4°C for 10 minutes using Bioruptor (Diagenode). Run-on reactions were performed at 37°C for 5 minutes using biotinylated nucleotides (5 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated 10 minutes at 4°C and then sheared using a Bioruptor (Diagenode, Belgium) for 4 times at 5 cycles and followed by 2 cycles at 30″ ON/OFF high power ...
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bioRxiv - Cell Biology 2024Quote: ... the reverse crosslinking (5 h at 65°C) and elution were performed using IPure kit (Diagenode). The final elution was performed in 60 μL ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequently disrupted by 2 × 20” high intensity sonication cycles at 4°C in a BioRuptor (Diagenode). Protein concentration was determined using the Micro BCA™ protein assay kit according to manufacturer’s instructions (Thermo Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were disrupted by sonication (two cycles à 20 s at 4 °C) in a BioRuptor (Diagenode), and extracts were cleared by centrifugation at 15,000 x g for 1 min ...
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bioRxiv - Genetics 2021Quote: ... incubated on ice for 10 min and subsequently sonicated at 4 °C with a Bioruptor sonicator (Diagenode) at high power for 5 cycles with 30 s ON and 30 s OFF ...
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bioRxiv - Genomics 2022Quote: ... the nuclei were briefly sonicated for 5 seconds at 4°C in a Bioruptor Pico sonicator (Diagenode). Permeabilized nuclei were incubated for 10 minutes at 37°C in a thermoshaker ...
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bioRxiv - Microbiology 2023Quote: ... the different antibodies were previously incubated at 4°C with protein G-coated magnetic beads (DiaMag, Diagenode), protease inhibitor cocktail and 0.1% BSA ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was then placed in a thermostated (50°C) ultrasonic bath (Bioruptor Plus, Diagenode, Liège, Belgium); ultrasounds were applied at low power for 10 cycles (30 seconds on – 30 seconds off) ...
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bioRxiv - Genetics 2024Quote: ... The mixture was heated to 95°C for 10 minutes and sonicated in a Bioruptor (Diagenode, Belgium) (30 seconds sonication ...
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bioRxiv - Developmental Biology 2021Quote: ... snap frozen and stored at −80 °C or further processed using iDeal ChIP for Transcriptional Factors Kit (Diagenode) (Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... Cross-linking was reversed at 65°C overnight and immunoprecipitated DNA was recovered using the IPure Kit (Diagenode). Genomic regions of interest were identified by realtime PCR (qPCR ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154; H3K27me1: Diagenode: C15410045-50) diluted in 1% BSA ...
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bioRxiv - Developmental Biology 2024Quote: ... The sonication of samples was then performed at 4°C using a Bioruptor® Plus sonication device (Diagenode) at high-intensity settings ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was then incubated at 37 °C for 120 hours with constant agitation at 200 rpm and the product sonicated 10 min at 10 °C (0.5 min on/0.5 min off) using a Biorupter Pico (Diagenode). Aggregates were flash-frozen with liquid nitrogen in single-use aliquots for storage at -80 °C.
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bioRxiv - Physiology 2024Quote: ... and 500 µl volume) in a water bath sonicator set at 4°C (Bioruptor 300. Diagenode, Denville, NJ). After centrifuging at 10,000g for 10 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 × g ...
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Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell LinesbioRxiv - Systems Biology 2021Quote: ... SDS lysates were boiled at 95°C for 10 minutes and then 10 cycles in a Bioruptor Pico (Diagenode) of 30 seconds on and 30 second off per cycle ...
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Deep learning the collisional cross sections of the peptide universe from a million training samplesbioRxiv - Systems Biology 2020Quote: ... The samples were boiled at 95°C for 10 min and subsequently sonicated at maximum power (Bioruptor, Diagenode, Belgium). Proteolytic digestion was performed overnight at 37 °C by adding either i ...
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bioRxiv - Immunology 2020Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 xg ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then incubated at 4°C overnight with or without 12.5 μg of anti-5hmC antibody (C15220001, Diagenode) in freshly prepared 1X Immunoprecipitation (IP ...
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bioRxiv - Immunology 2022Quote: ... Sonicated chromatin was diluted ten times in ChIP Dilution Buffer and immunoprecipitation was done overnight at 4°C by incubating 2.5 μl (7 μg) of anti-H3K27ac antibody (C15410196; Diagenode) precoated on 25-μl protein A-coated magnetic beads (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... Primary antibody incubation was performed overnight at 4°C with gentle shaking using anti-H3K9me2 (Diagenode Cat. No. C15410060), Anti-Histone H3 (Sigma-Aldrich Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were sonicated (settings: 3 – 4 x 30 sec on /30 sec off, 4°C, power high; Bioruptor, Diagenode) and lysates were cleared by centrifugation at 16,000 x g at 4°C for 15 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended samples were boiled for 5 min at 95°C and sonicated for 15 min in the Bioruptor (Diagenode) at 30 sec on ...
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bioRxiv - Cell Biology 2023Quote: ... Chromatin was fragmented by sonication at 4°C using a Bioruptor 300 (Diagenode; 45 cycles, 30s on/30s off). Immunoprecipitation used 4 μg ChIP validated antibody against NF-κB p65 (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were sonicated for 5 cycles of 30 s on/off (2.5 min total sonication time) at 4°C in a Bioruptor Plus sonication system (Diagenode) and pelleted by centrifugation at 8000 RPM for 15 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with mixing for 30 min at 4°C before sonicating with a Bioruptor® Pico (Diagenode). Lysates were then spun for 10 min at 15,000 g ...
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bioRxiv - Cancer Biology 2024Quote: Hi-C libraries were built from 4 µg of DNA per sample and sonicated using a BioruptorPico device (Diagenode, cat ...
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bioRxiv - Molecular Biology 2020Quote: ... was then added at a final concentration of 1 % for 15 min at RT and samples were sonicated over 20 min (10 × 30 s pulses) at 4°C to cleave genomic DNA (Bioruptor, Diagenode). Anti-MAZ antibody was from Bethyl Laboratories (A301-652A) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split into 150 μl aliquots in 0.5 ml Eppendorf tubes and sonicated at 4°C for 30 cycles (30 seconds on/off) using a Bioruptor (Diagenode). Aliquots of the same samples were pooled and spun down at 12,000xg for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 30 min at 4°C with rocking and then sonicated (3 x 30 sec interval, high setting in a Bioruptor water bath sonicator, Diagenode). Lysates were cleared by centrifugation at 13,000 g for 15 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and for 15 minutes at 100°C in a proteinase K/elution buffer mix (1 µl of proteinase K in 100 µl DIB-buffer; Diagenode). Capture efficiency was determined by qPCR against spiked-in Lambda-DNA fragments in precapture and postcapture library samples ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitation was performed using IP star robot at 4°C (indirect 200µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10μg ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 95°C for 5 min and sonicated at 4°C for 2 cycles of 20 s at high intensity level using a BioRuptor (Diagenode). Samples were clarified by centrifugation at 16,000 x g for 15 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were sonicated at 4° C for 20 minutes using a waterbath sonicator cycling 30 seconds on and 30 seconds off (Bioruptor Pico, Diagenode). Sonicated lysates were cleared by centrifugation at 18,000 g for 10 minutes at 4° C ...
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bioRxiv - Molecular Biology 2020Quote: ... with minor modifications: Cells were resuspended in PBS at 4°C after flow cytometry sorting and incubated with ChIP Crosslink Gold (Diagenode #C01019027 ...
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bioRxiv - Neuroscience 2020Quote: ... PFFs are diluted in PBS and sonicated for 10 cycles (1 sec ON, 30 sec OFF, high intensity) in a bath sonicator at 10°C (BioRuptor; Diagenode).
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bioRxiv - Genomics 2021Quote: ... Lysates were sonicated for 15 cycles (30 sec on / 30 sec off) at 4°C using a Bioruptor Pico sonicator (Diagenode). After sonication ...
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bioRxiv - Cell Biology 2022Quote: ... cells were sonicated for 10 cycles of 30 sec “on” and 30 sec “off” at 4°C with Bioruptor (Diagenode) and the protein concentration was measured using Bradford protein assay (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... samples were sonicated for 15 min (30 sec on, 30 sec off at 10°C) with a Bioruptor Pico (Diagenode). Then ...