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Citations for Diagenode :
51 - 100 of 123 citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Samples were heated at 72 °C for 5 min and sonicated using a Bioruptor (Diagenode Inc) with fifteen 30 s on/off cycles at maximum intensity ...
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bioRxiv - Molecular Biology 2022Quote: ... After sonication for 5 cycles of 30 seconds ON/OFF at 4 °C (Bioruptor Pico, Diagenode), the mixture was centrifuged (13 000xg ...
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bioRxiv - Genomics 2022Quote: ... Proximity ligated chromatin pellet was sonicated and immunoprecipitated using 5 μg of H3K27ac antibodies (Diagenode, C15410196). Immunoprecipitated fragments were Biotin labeled (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: ... single-stranded DNA mixture was incubated with 5-mC antibody provided by MagMeDIP-seq Package (Diagenode), followed by 14-16 cycles of library amplification ...
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bioRxiv - Cancer Biology 2023Quote: ... was used and 5 cycles sonicated then (Bioruptor® Plus and Bioruptor® Next Gen, Diagenode). Then supernatant was diluted to dilution buffer 2.5 times ...
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bioRxiv - Cell Biology 2024Quote: ... the reverse crosslinking (5 h at 65°C) and elution were performed using IPure kit (Diagenode). The final elution was performed in 60 μL ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed using 75 µg of chromatin and 5 µg of Cas9 antibody (Diagenode #C15200229-100) or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913 ...
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bioRxiv - Developmental Biology 2021Quote: 5×105 fixed nuclei were sonicated to a 200-500bp length with the Bioruptor Pico sonicator (Diagenode). H3K27Ac ChIP was (Diagenode C15410174 ...
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bioRxiv - Molecular Biology 2021Quote: ... then the sample was sonicated at a high intensity (3 × 5 min) using a Bioruptor sonicator (Diagenode). To obtain organoid-MPF cocultures ...
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bioRxiv - Genomics 2022Quote: ... the nuclei were briefly sonicated for 5 seconds at 4°C in a Bioruptor Pico sonicator (Diagenode). Permeabilized nuclei were incubated for 10 minutes at 37°C in a thermoshaker ...
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bioRxiv - Plant Biology 2022Quote: ... Cells were ruptured by 5 × 15 sec sonication in an ice bath in Bioruptor UCD-200 (Diagenode) at medium intensity with 2 min breaks on ice ...
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bioRxiv - Genetics 2024Quote: ... 5 μg of genomic DNA from each treatment condition was sheared using a bioruptor sonication device (Diagenode) in low mode for two cycles (30s on + 60s off ...
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bioRxiv - Molecular Biology 2023Quote: ... and then sonicated for 5 min at high setting with 30sON/30sOFF in a Bioruptor sonicator (Diagenode) and centrifuged for 5 min at 19000g at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The pellet was resuspended in 5 volumes of washing buffer and sonicated in a Bioruptor Plus (Diagenode) for 5 cycles of 30 seconds ON ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates underwent 5 minutes of sonication with on/off intervals of 30 seconds using a Bioruptor (Diagenode), followed by centrifugation at 20,000 g for 10 minutes to retrieve the protein supernatant ...
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bioRxiv - Cancer Biology 2024Quote: ... and sonicated for 5 cycles (30 s ON, 30 s OFF) with the Bioruptor Pico sonicator (Diagenode). Chromatin was cleared by centrifugation (13,000 rpm for 10 min at 4 C) ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended nuclei were sonicated 5 times 30 sec on followed by 30 sec off using the Bioruptor (Diagenode). Insoluble proteins were removed by centrifugation for 10 min at 14,000 rpm at 4 °C.
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bioRxiv - Cancer Biology 2021Quote: ... Adapter-ligated DNA was subjected to immunoprecipitation with a primary monoclonal antibody against 5-methyl cytosine (Diagenode C15200081) using a previously published protocol [71] ...
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bioRxiv - Genomics 2023Quote: ... 5 × 105 fixed nuclei were sonicated to a 200–500 bp length with the Bioruptor Pico sonicator (Diagenode). H3K27Ac ChIP (Diagenode ...
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bioRxiv - Cell Biology 2024Quote: ... Chromatin corresponding to 5 μg of DNA was incubated with 2 μg of anti-H3K4me3 (Diagenode; Cat#: C15410003) or anti-H3K9me3 (Diagenode ...
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bioRxiv - Plant Biology 2024Quote: ... Chromatin sonicated 3 times for 5 min (30 sec on/off intervals) in a Bioruptor® Plus (Diagenode) at the low setting to generate DNA fragments in the range of 200-800 bp ...
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bioRxiv - Neuroscience 2021Quote: ... the adaptor-ligated DNA was used for immunoprecipitation (IP) with a mouse monoclonal anti-5-hydroxymethylcytosine antibody (Diagenode, C15200200). For this ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (5 cycles, each 30 s on and 30 s off) using a Biorupter (Diagenode). A centrifugation step (at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Crosslinking was terminated by the addition of 1/10 volume 1.25 M glycine for 5 min at room temperature followed by cell lysis and sonication (Bioruptor, Diagenode), resulting in an average chromatin fragment size of 200 bp ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended samples were boiled for 5 min at 95°C and sonicated for 15 min in the Bioruptor (Diagenode) at 30 sec on ...
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bioRxiv - Microbiology 2023Quote: ... 40 mM 2-Chloroacetamide, 50 mM HEPES pH 8.5), boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). For the intracellular proteomes ...
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bioRxiv - Molecular Biology 2023Quote: ... High Molecular Weight Genomic DNA (5 µg) was sheared with the 40 kb program using a Diagenode Megaruptor (Diagenode) to generate DNA fragments of approximately 30 kb ...
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Spirolactone, an unprecedented antifungal β-lactone spiroketal macrolide from Streptomyces iranensisbioRxiv - Biochemistry 2024Quote: ... sonicating on high for 5 x 60 sec on/30sec off using the bioruptor pico sonication water bath (Diagenode) and finally disrupting twice with the tissuelyser (QIAGEN ...
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bioRxiv - Genomics 2024Quote: ... 5 μl of each annealed A and B transposome were combined with 10 μl of unloaded Tagmentase (Diagenode, C01070010), briefly vortexed ...
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bioRxiv - Cell Biology 2024Quote: ... After 2 × 5 cycles of sonication (30 s “ON”, 30 s “OFF”, High intensity) with a Bioruptor UCD200 (Diagenode), the chromatin solution was diluted 10-fold to a final volume of 3 mL with ChIP dilution buffer before IP ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split into 150 μl aliquots in 0.5 ml Eppendorf tubes and sonicated at 4°C for 30 cycles (30 seconds on/off) using a Bioruptor (Diagenode). Aliquots of the same samples were pooled and spun down at 12,000xg for 5 min ...
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bioRxiv - Immunology 2020Quote: ... chromatin was sheared using a Bioruptor Pico Sonicator (5 cycles: 30 seconds ON - 30 seconds OFF, fragments 100 - 300 bp, Diagenode) and 10 % of sheared chromatin was kept as input control whilst 90 % was immunoprecipitated overnight using antibodies against H3K27ac ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation at 95C for 5 min the sample was sonicated 3 times for 30 sec on/off with a Bioruptor (Diagenode) on high power ...
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bioRxiv - Cell Biology 2020Quote: ... assisted by sonication (5 cycles of 30 s ON, 30 s OFF at high power, Bioruptor Plus ultrasonic disintegrator, Diagenode). Protein concentration was measured by the BCA assay.
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bioRxiv - Developmental Biology 2022Quote: ... in VFG media for 10 minutes at room temperature followed by a 5-minute quench with glycine (in True MicroChIP kit, Diagenode) at room temperature ...
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bioRxiv - Genomics 2022Quote: ... and chromatin was fragmented into 1–5-kb fragments by sonication (high level, 33 cycles, 30 s ON, 50 s OFF) using a Bioruptor (Diagenode). Chromatin immunoprecipitation was used to enrich the complex fragments with magnetic beads of protein G (Thermo ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Pathology 2021Quote: ... and fragmented by 5 cycles of sonication on a Bioruptor (per cycle: power = high, interval = 30s on/30s off, 5min; Diagenode) to facilitate efficient DNA clustering onto the Illumina sequencing platform ...
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bioRxiv - Cancer Biology 2021Quote: ... the remaining pellet was resuspended in 50μl RIPA supplemented with 1x Protease Inhibitor Cocktail and sonicated for 3-5 min with Picoruptor® (Diagenode). 10μg of proteins were then separated through precast 4-12% NuPAGE® Bis-Tris gels (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: MeDIP-Seq libraries for Ion Torrent semiconductor sequencing were performed on the Ion Torrent PGM using a modified protocol from Ion Plus Fragment Library kit (Catalog # 4471252) combined with a 5-hydroxymethylcytosine (5hmC Kit, Catalog # AF-110–0016) immunoprecipitation kit (Diagenode) according to a previously optimized protocol (Guerrero-Bosagna & Jensen ...
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bioRxiv - Molecular Biology 2022Quote: ... were sonicated with 5 cycles of 30 s on followed by 30 s off at 4°C (Bioruptor Plus, Diagenode). After the centrifugation for 15 min at 4°C and 16,100 ×g ...
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bioRxiv - Bioengineering 2024Quote: ... The cell lysates were heated at 95[ for 5 minutes and sonicated for 15 min using a Bioruptor Plus (Diagenode). The supernatant was collected after centrifugation at 18,000 g for 30 min at 4[ ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2mM EDTA) and briefly sonicated to remove flagella (ON 5 sec – OFF 30 sec x 3 Cycles, bioruptor Pico, Diagenode). The samples were transferred in low retention tubes ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysis was obtained by pulsed ultrasonication (5 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300). Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 ml of thawed OFs were sonicated with a closed-type sonication system (UCD-200T, Biorupter, Diagenode Inc., NJ, USA) and were sequentially centrifuged at 300 g for 10 min (model 5500 with tube 430829 ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to the supernatant and sonicated for 30 s ON/OFF for 5 min at high power in Bioruptor (Diagenode). After centrifugation at 16,000 × g for 10 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... was dissolved in 75 µL lysis buffer, boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). Lysates from the secretomes and intracellular fractions were centrifuged (10 min ...