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Citations for Greiner :
201 - 243 of 243 citations for Recombinant Human Neuropilin 1 Fc Chimera since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The cell-pellet was then resuspended in 1 ml of TR salts medium and 5 µl of this suspension inoculated into 195 µl TR medium with either carbon source (∼105 colony forming units (CFUs ml−1) in a 96-well plate (Greiner Bio). The optical density (600 nm ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were washed twice with PBS and mixed together in 1 ml PBS in a 24-well plate (Greiner Bio-One GmbH) at a final OD600 of 0.7 for S ...
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bioRxiv - Cell Biology 2023Quote: ... 900 µl of cell suspension containing 1 × 10 ⁶ cells were kept in a 15 mL tube (Greiner Bio-One, Kremsmünster, Austria), which was positioned horizontally before 100 µl of CTV staining solution was added to the upper end of the tube without mixing ...
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bioRxiv - Microbiology 2020Quote: ... SupT1-R5 cells with AAV (expressing CPSF6 shRNA or non-silencing control shRNA) were distributed at 72 h post-transduction into 96-well plates (1 × 105 cells/well; U-bottom; Greiner Bio-One) and infected with wild-type HIV-1 (1 μUnits of RT/cell) ...
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bioRxiv - Biochemistry 2020Quote: ... 1-2 μl of beads per sample were pipetted into the wells of a 384-well glass-bottom microplate (Greiner Bio-One) pre-filled with a prep of ATTO390-SUVs in buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... A suspension of Ins-1 cells was pipetted in a 384-well culture black microplate with optic bottom plate (Greiner-Bio, 781090) at ~17,000 cells/well in 25uL of the low glucose DMEM culture medium ...
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bioRxiv - Genomics 2020Quote: Overnight cultures were diluted 100-fold in 1 mL of LB in a 96-deepwell plate (Masterblock 96 well, 2ml, V-bottom plates by Greiner Bio-one). After 2.5 h at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... Confrontations were carried out by spreading mixtures of both interaction partners at prey-predator ratios of 10:1 on 20 x wMY agar-plates (120 x 120 x 17 mm, Greiner Bio-One). At indicated time points cells were washed off the plate and centrifuged at 3000 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... Serially diluted inhibitor and Tracer molecule 19c at a concentration of 1 µM determined previously as the Tracer 19c KD,app were pipetted into white 384-well plates (Greiner 781 207) using an Echo acoustic dispenser (Labcyte) ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of RPMI-1640 was added to all the wells of columns 1-9 and 12 of a flat-bottom 96-well microtiter plate (Greiner Bio-One). 200 μL of RPMI-1640 + 12.8 mg/mL BA was added to all wells of column 11 and 200 μL of RPMI-1640 + 64 μg/mL FLC was added to all wells of column 10 ...
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bioRxiv - Immunology 2022Quote: ... NR-54970) were seeded at a density of 1×104 per well in half area 96-well black opaque plates (Greiner Bio-One) and were grown overnight at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Plant Biology 2021Quote: Seedlings were initially grown on 1/2 MS plates for 3 days before being transferred to 96-well plates (655075, Greiner Bio-One) in 100 μL liquid MS for 5 days ...
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bioRxiv - Plant Biology 2021Quote: Four-day-old seedlings growing on 1/2 MS plates were transferred into individual wells of a transparent 48-well tissue culture plate (Greiner Bio-One) containing 500 μL of liquid MS media with/without elicitor addition ...
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bioRxiv - Cell Biology 2019Quote: ... 50 mM Tris·HCl pH 7.4 and 1 mM DTT in a 20 µl volume in non-binding clear bottom 384 well plates (Greiner Bio-One, 781906). Compounds ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cultures were diluted 1:100 into 1 mL of LB/chloramphenicol medium with different concentration of bile salts in 96 deep-well plates (Greiner bio-one), incubated at 37°C with vigorous shaking for a further 4 hours and analyzed by flow cytometry ...
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bioRxiv - Microbiology 2022Quote: ... Filtrate and diluted concentrate (1:100) were added to a black optical bottom 96 well plate (10 µL/well; Greiner Bio-One) in technical duplicate and each fraction was processed separately in the presence or absence of hydrolysis enzymes according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Concentration of cells in the medium was estimated using a Neubauer chamber and a 100 μL of medium containing 1×106 cells /mL plated per well in a 96-well plate (Greiner Sensoplate, Germany) coated with Poly-D-Lysine (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5 µL of cells at 1 × 105 cells/mL (500 cells/well) were dispensed into a 1536-well white solid-bottom tissue treated plate (Greiner Bio-One) using a Multidrop Combi dispenser ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cells were then inoculated at the starting OD600= 1 in SDG medium using a six-well microplate (Greiner Bio-One™) and were incubated at 30 °C and 350 rpm in a thermomixer for 48 hours ...
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bioRxiv - Bioengineering 2023Quote: ... a 1:1 volume of reagent and culture medium was added to each sample of a black 96 well plate (655892, Greiner Bio-one), shaken for 10 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... or DMSO (columns 1-2, 23-26, and 47-48) was dispensed into 1536 well black polystyrene assay plates (Greiner, Kremsmünste, Austria) using an Echo 550 liquid dispenser (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: The stable cell line of SNAPf–TDP-43 was seeded in complete DMEM containing 10% FBS and 1× PSQ at a density of 5 ∼ 6 × 105 cells/dish in cell culture dishes (35 mm, Greiner Bio-One). Stock solutions of doxycycline (2 mg/mL in DMSO) ...
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bioRxiv - Cell Biology 2023Quote: ... 100 μl of cells was vigorously mixed with 100 μl of 1 mM D-luciferin in a white 96-well plate (655073, Greiner Bio-One), and light emission was immediately measured by the luminescence detection mode in Cytation 5 (Biotek) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were counted by hemacytometer then seeded at a density of 1 million cells per well in 6-well poly-D-lysine pre-coated plates (Greiner Bio-One) and incubated at 37°C ...
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bioRxiv - Immunology 2024Quote: ... S-Fuse cells were mixed (at a 1:1 GFP1-10 / GFP11 ratio) and plated at 8 × 103 cells per well in a μClear 96-well plate (Greiner Bio-One). The tested SARS-CoV-2 strains were incubated with serially diluted chip effluent or supernatants from ECM digestion for 20 min at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and HSJD-GBM-001 (GBM1) were cultured in suspension in low-attachment 250 mL/75 cm2 flasks (Greiner #658, Supp. Table 1). The medium for DIPG4 ...
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bioRxiv - Biophysics 2020Quote: ... were collected by centrifugation at 114 RCF for 5 min and suspended in 1 ml of fresh medium in a cell culture-treated 12-well plate (#665180, Greiner Bio-One, Austria). The starting cell count was equal to 70,400 for target sample ...
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bioRxiv - Molecular Biology 2021Quote: ... and mixed with biotinylated RBD protein (final concentration 1 nM) in white low binding 384well microtitre plates (F-bottom, Greiner Cat nr 781904). As isotype control antibody ...
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bioRxiv - Pathology 2021Quote: ... diluted to a concentration of 1 mM and 40 nL were dispensed in 384 well plates (Greiner µClear #781091, Greiner Bio-One GmbH) using an acoustic liquid handler (Echo® 550 ...
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bioRxiv - Pathology 2021Quote: ... diluted to a concentration of 1 mM and 40 nL were dispensed in 384 well plates (Greiner µClear #781091, Greiner Bio-One GmbH) using an acoustic liquid handler (Echo® 550 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were prepared by aliquoting 180 μL of gel into ThinCert well inserts (1 μM pore size, Greiner Bio-One Ltd, Gloucestershire, UK). 900 μL of assay media was pipetted into the wells after 5 minutes and left to recover for 5 minutes before 100 μL of media was added to each insert ...
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bioRxiv - Biochemistry 2024Quote: ... After 1 µl of protein solution (10 mg/ml) was mixed with 1 µl of reservoir solution on a sitting plate (96-well CrystalQuick plates, Greiner Bio-One, Germany), the plate was incubated at 20 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were anesthetized in 0.015% tricaine- S and embedded dorsally in 1% LMP agarose in E3 medium + PTU in a lumox membrane-bottomed dish (Greiner Bio-one, Monroe, NC). Images were acquired on a Leica TCS SP8X laser-scanning confocal microscope equipped with LASX software ...
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bioRxiv - Cell Biology 2019Quote: ... 200 μL of this diluted culture was diluted 1:10 into YPD four times in a sterile 96-well plate (Greiner Bio-one, Kremsmünster, Austria). 5 μL of the serial dilutions were transferred from the 96-well plate onto YPD agar plates with or without additional 5 mM guanidinium hydrochloride (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... albicans cells were prepared in 0.05×PDB at a concentration of 1×106 cells/mL and 100 μL was added to each well of a black polystyrene microtiter plate (Greiner Bio-One, Frickenhausen, Germany). Fluorescence intensity was followed for 7 min 30 sec at an excitation/emission wavelength of 622/670 nm using a multimode microplate reader (CLARIOstar Plus ...
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bioRxiv - Microbiology 2019Quote: ... were subcultured at a ratio of 1:2 every 2 - 4 weeks and maintained in 25 cm2 culture flasks (Cellstar; Greiner Bio-One, Monroe NC) at 32°C ...
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bioRxiv - Neuroscience 2021Quote: ... were seeded at a density of 1 × 106 cells/well onto tissue culture inserts containing porous membrane (1 µm pores; Greiner Bio-One GmbH, Kremsmünster, Austria) in a 6-well cell culture plates ...
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bioRxiv - Systems Biology 2022Quote: ... We picked three single colonies for each strain and inoculated them into separate wells containing 200 μL of LB (with 50 mg L-1 kanamycin for transposon insertion strains) in 96-well flat-bottom polypropylene plates (Greiner Bio-One ref: 655261) and incubated them at 30 °C overnight shaking at 800 rpm.
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bioRxiv - Biochemistry 2023Quote: ... 150,000 to 200,000 HEK293-EBNA cells were plated in 1 mL complete DMEM per well of 12-well cell culture plates (#665180, Greiner bio-one, Merck KGaA). After 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... vivax cases were infected into PHH lot BGW at day 2 post-seed (for case 1) or day 3 post-seed (for cases 2 and 3) in 384-well plates (Greiner Bio-One cat 781956) using the same methods for initiating P ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were washed in 1%FBS in Dulbecco’s PBS 3 times before straining through 75 µm and 30 µm strainers (Greiner Bio-One and Miltenyi Biotec) in Dulbecco’s PBS ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted and washed with DSM 457 lacking a carbon source before being diluted 100-fold into fresh medium containing the appropriate carbon source and grown as triplicate 1 mL cultures in 48-well plates (Greiner Bio-One, Kremsmünster, Austria). The growth was monitored by measuring the optical density at 600 nm (OD600 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A new 18G butterfly catheter attached to a new 3 mL syringe was then used to draw the experimental 1-2 mL blood sample and was immediately transferred to EDTA tubes (Greiner Bio-One, Monroe, NC, USA) at times 0.25 ...