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Citations for Greiner :
1 - 50 of 62 citations for Recombinant Human Epidermal Growth Factor Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... recombinant human TNFa (Shanghai Primegene Bi-Tech) was coated onto microtiter plates (Greiner Bio-One) in 0.05M carbonate-bicarbonate buffer overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 nM of recombinant human G6PD) in a dark 96-well plate (Greiner Bio-one). After 1 min incubation in the dark at 28◦C ...
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bioRxiv - Microbiology 2022Quote: ... and 1:750 for Fcγ-receptor binding) in a 384-well plate (Greiner, Germany) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: For growth curves in 96-well microplates (Greiner #655180), bacteria grown beforehand for ∼6 hours in LB ...
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bioRxiv - Microbiology 2023Quote: Growth assays were conducted in 96-well microtiter plates (Greiner), with each well containing 100 µl YP [2% peptone (Oxoid) ...
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bioRxiv - Cell Biology 2022Quote: ... in T-175 tissue culture flasks (Greiner, growth surface 175 cm2). When confluency was reached ...
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bioRxiv - Microbiology 2021Quote: ... Growth assays used 200μL culture volumes in 96-well plates (Greiner) sealed using Breathe-Easy® membranes (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Growth assays used 200μL culture volumes in 96-well plates (Greiner) sealed using Breathe-Easy® membranes (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Growth assays used 200μL culture volumes in 96-well plates (Greiner) sealed using Breathe-Easy® membranes (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... and 1:750 for Fcγ-receptor binding) overnight at 4°C in 384 well plates (Greiner Bio-One, Germany). Secondary antibodies were PE-conjugated and incubated with samples at room temperature for 1 hour at a 1:100 dilution in sterile-filtered Assay Buffer (1X PBS ...
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bioRxiv - Immunology 2022Quote: ... and 1:750 for Fcγ-receptor binding) overnight at 4°C in 384 well plates (Greiner Bio-One, Germany). Unbound antibodies were removed by washing and subclasses ...
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bioRxiv - Biochemistry 2024Quote: ... we measured directional reflectance and transmittance factors through the procedure above (Greiner et al. 2007), and the terms leaf reflectance and transmittance were used here for expediency.
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bioRxiv - Cell Biology 2023Quote: The cell suspension containing DNA (BRET/EbBRET biosensors and receptors) were seeded in white 96-well plates (Greiner, Stonehouse, UK) at 30,000 cells/well (100 μl per well) ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant HIV-1 Gp120 was immobilized on high-binding ELISA plates (Greiner #655081). Plates were washed with PBS-T and blocked with a solution of 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... His-SLUG or BSA diluted in PBS were added to a 96-well plate (Greiner Microlon) and incubated for 1 hour at room temperature followed by blocking with 3% BSA for 30 min ...
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bioRxiv - Immunology 2023Quote: ... Antigen-coupled microspheres were washed and incubated with plasma samples at an appropriate sample dilution (1:50 for Isotypes and 1:100 for all Fc-receptors) for 2 hours at 37°C in 384-well plates (Greiner Bio-One). Unbound antibodies were washed away ...
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bioRxiv - Biophysics 2022Quote: Measurements of bacterial growth were performed using 96-well plates (Cellstar transparent flat-bottom, Greiner). Overnight cultures were inoculated at an initial OD600 of 0.01 in the same medium as used for growth in other experiments ...
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bioRxiv - Cell Biology 2021Quote: The collection of yeast strains with N’-GFP tagged peroxi-ome proteins and Pex11-mScarlet were transferred manually from agar plates into 384-well polystyrene plates (Greiner) for growth in SD-URA liquid media ...
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bioRxiv - Molecular Biology 2024Quote: ... INTS13-14 complex at concentrations ranging from 0.25 nM to 4 μM were briefly incubated with the GFP-tagged peptide in a black 384-well plate (Greiner) in a total reaction volume of 30 µL ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were passaged into roller bottles (CellMaster®, Greiner Bio-One, growth surface 2125 cm2). The old medium was removed ...
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bioRxiv - Cell Biology 2020Quote: ... supplied with 1ml in-house-generated granulocyte-macrophage colony stimulating factor (GM-CSF) into 94mm petri dishes (Greiner, 632180). At day 3 ...
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bioRxiv - Microbiology 2022Quote: ... Growth took place in 96-well cell culture plates (F-bottom; Greiner Bio-One, Fischer Scientific, US) with 200 μL working volume in thermostated plate-shakers at 30 °C and 600 rpm (PST-60HL ...
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bioRxiv - Microbiology 2022Quote: ... or 40 µgmL-1tetracycline (for selection of the DsRed-tagged strains) in the well of a 48-well plate (Greiner Bio-One) ...
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bioRxiv - Microbiology 2020Quote: ... histolytica trophozoites maintained in the logarithmic phase of growth were seeded into 96-well plates (Greiner Bio-One) at 5,000 cells/well to a total volume of 100 μl/well ...
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bioRxiv - Microbiology 2019Quote: ... Nearly all fitness assays were performed in 1.2 mL of growth medium in either a 24-well transparent microplate (Greiner) or in a 96-deepwell plate (Costar) ...
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bioRxiv - Cell Biology 2023Quote: IMR32 cells were plated in 40 μL of growth medium (5000 cells/well) in white 384-well plates (Greiner). The next day each well was transfected with 100 ng pTI-ARE-LUC using FuGENE HD (4 μL per ug DNA ...
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bioRxiv - Immunology 2019Quote: ... The cells were resuspended in growth medium and transferred to a 96-well U bottom plate (Greiner BioOne, Cellstar, #650180) and harvested by centrifugation ...
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bioRxiv - Microbiology 2020Quote: Isolates growth curves were determined from Brain Heart Infusion broth (BHI) cultures incubated in 96-well flat bottom plates (Greiner) for 24 h at 37 °C with continuous optical density monitoring at 600 nm (Tecan Infinite® 200 PRO) ...
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bioRxiv - Microbiology 2022Quote: In vitro growth curves were obtained by growing 200 µL of Xcc suspensions in 96-well flat-bottom microtiter plates (Greiner) within a FLUOStar Omega apparatus (BMG Labtech ...
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bioRxiv - Synthetic Biology 2023Quote: ... The 20 µL of pre-cultured cell sample was mixed with 180 µL of SC–His–Leu–Ura+Ade media and transferred into a flat-bottom transparent 96-well plate (Greiner Bio-One #655090). Samples were then analyzed using Infinite 200 PRO plate reader (TECAN ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl Nano-Glo HiBiT Lytic Reagent containing 1 μl furimazine and 2 μl recombinant LgBiT were added to 50 μl lysate in a white flat-bottom 96 well plate (Greiner) in technical triplicates ...
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bioRxiv - Plant Biology 2023Quote: ... individual fronds of Lemna minor were placed in 100 μl of growth media per well in black 96 multi-well plates (Greiner Bio-one) displaying a low auto-fluorescence thereby minimizing interference with the Chl fluorescence signal detection.
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bioRxiv - Microbiology 2022Quote: Human blood from healthy volunteers was collected in plastic vacuette tubes (Greiner) with informed consent in accordance with the Declaration of Helsinki ...
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bioRxiv - Immunology 2023Quote: ... After 7 days of cell culture were plated in a density of 17-20 × 106 cells/mL in BMDM medium supplemented with 5ng/mL of recombinant mouse macrophage colony stimulating factor (mCSF, R&D Biosystems, Minneapolis, USA, 416-ML-500) onto non-treated 100mm tissue culture Petri dishes (Greiner Bio-One, St. Gallen, Switzerland) for 7 days at 37°C and 5% CO2 the cells were harvested by incubation with 0.05% Trypsin.
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bioRxiv - Cell Biology 2022Quote: 2’500 siRNA transfected cells were seeded 24h after transfection per well in 100 µl of standard growth media in cell-repellent 96 well microplates (Greiner Bio-one, 650790). Plates were incubated 24 hours at 37°C in 5% CO2 to allow spheroid formation ...
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bioRxiv - Microbiology 2021Quote: ... grown overnight in 50 µL of D10 growth media in a 96-well black-walled poly-L-lysine coated plate (Greiner Bio-One, 655936). Relative luciferase units (RLU ...
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bioRxiv - Microbiology 2021Quote: ... grown overnight in 50 μL of D10 growth media in a 96-well black-walled poly-L-lysine coated plate (Greiner Bio-One, 655936). Relative luciferase units (RLU ...
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bioRxiv - Microbiology 2022Quote: ... grown overnight in 50 µL of D10 growth media in a 96-well black-walled poly-L-lysine coated plate (Greiner Bio-One, 655936). Relative luciferase units (RLU ...
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bioRxiv - Microbiology 2023Quote: ... was incubated with 50µL of magnetic beads coated with recombinant protein (2 µg protein/1.25 × 106 beads) in 96-well flat-bottom chimney plates (Greiner bio one, Frickenhausen, Germany) on a plate shaker at 300 rpm for 16 h at 4 °C in the dark ...
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bioRxiv - Immunology 2022Quote: ... two-fold serial dilutions of recombinant Bma-LEC-1 and Bma-LEC-2 were prepared in V-bottom 96-well plates (Greiner Bio-One, Kremsmünster,Austria) followed by the addition of a 4% solution of trypsinized ...
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bioRxiv - Microbiology 2021Quote: The growth of different strains on different aromatic substrates was tested by cultivations in 96- well plates (Greiner Bio-One™ CellStar™ μClear™). The cells were pre-cultivated in MSM supplemented with 5 mM aromatic substrate (ferulate/vanillate/ p-coumarate ...
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bioRxiv - Neuroscience 2022Quote: ... human 3DNAs were seeded and cultured on PLO/Laminin-coated 96-well plates (Greiner) and cultured in BrainPhys media with supplements (23) ...
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bioRxiv - Microbiology 2023Quote: ... Human cells ( co-cultured) were harvested from 6-well plates (Greiner Bio-One, Austria) or small petri dishes (diameter of 3 cm ...
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bioRxiv - Immunology 2022Quote: ... human naïve Tconv and tTreg cells were cultured in 96-well round bottom plates (Greiner) at 1 × 104 cells per well using IMDM (Gibco ...
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bioRxiv - Bioengineering 2021Quote: Human neuroblastoma cells SH-SY5Y (ATCC) were grown in tissue culture flasks (Greiner, Stroudwater, UK) containing high glucose Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: Human iPSC-derived cortical neurons and HEK293T cells were seeded in 6 well plates (Greiner) at a density of 2*106 cells and 0.5*106 cells per well ...
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bioRxiv - Genomics 2023Quote: Human PBMCs were prepared from the whole blood of a male healthy donor with LeucosepTM (Greiner®). Isolated 2×106 PBMC cells were incubated with PMA/ionomycin (i.e ...
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bioRxiv - Microbiology 2020Quote: ... and human bone osteosarcoma epithelial (U2OS) cells were cultured as adherent cells directly on tissue culture plastic (Greiner) in DMEM growth medium (high glucose ...
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bioRxiv - Genomics 2021Quote: Human PBMCs were prepared from the whole blood freshly collected from a male healthy donor with Leucosep (Greiner). Isolated 2×106 PBMC cells were incubated with PMA/ionomycin (i.e. ...
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bioRxiv - Immunology 2023Quote: For M1 EpXT analysis IgGs from IVIG and human plasma were purified in a 96 well plate (Greiner) using the Protein G AssayMAP Bravo (Agilent ...