Labshake search
Citations for Greiner :
1 - 50 of 56 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... in a 50 ml tube (Corning 352070) (when having a frozen cell pellet as starting material, 3 ml of washing buffer and 15 ml tubes [Greiner Bio-One 188-271] were used). The debris and leaking RNA were removed by centrifugation (500 g ...
-
bioRxiv - Biochemistry 2020Quote: ... Solutions were transferred to a 96-well plate (Greiner Bio-one), and fluorescence polarization was measured in a Tecan M1000-Pro microplate reader (Tecan ...
-
bioRxiv - Cancer Biology 2024Quote: ... the solution was transferred to an opaque 96-well plate (Greiner) and the fluorescence was measured on a Filtermax F5 multi-mode plate reader ...
-
bioRxiv - Cell Biology 2021Quote: ... solutions were transferred to white 96-well plate (Greiner Bio-One, Germany) and luminescence intensity was measured ...
-
bioRxiv - Genomics 2021Quote: ... Solution was then filtered through a 40-μmcell strainer (Greiner Bio-One), and propidium iodide was added at a final concentration of 5 ul/ml ...
-
bioRxiv - Microbiology 2023Quote: ... Homogenized solutions were transferred to white flat-bottom 96-well plates (Greiner Bio-One ...
-
bioRxiv - Molecular Biology 2020Quote: ... prepared as 2 μg/ mL solutions in black-bottom 96-well plates (Greiner), with 100 μL total volume per well ...
-
bioRxiv - Molecular Biology 2023Quote: ... The resulting solution was transferred to a black 96-well microplate (Fluotrac, Greiner) and fluorescence was measured by BMG Clariostar fluorimeter (BMG Latec ...
-
bioRxiv - Microbiology 2024Quote: ... The resulting solution was added to a black-walled flat-bottom 96-well plate (Greiner) and incubated at 30°C in a shaking plate incubator protected from the light ...
-
bioRxiv - Cancer Biology 2024Quote: ... Compound solution (50 nL) was added to 384-well assay plates (Greiner, catalog no. 784075). To each well containing compound solution was added assay buffer solution (5 µL) ...
-
bioRxiv - Bioengineering 2023Quote: ... This solution was then transferred to a dark 96 well plate (Greiner Bio-one, Germany) for fluorescence reading by excitation at 484 ± 10 nm and emission at 518 ± 10 nm wavelengths in a microplate reader (FLUOstar Omega ...
-
bioRxiv - Cell Biology 2024Quote: ... 70 µl of collagen solution per well was loaded into a 96-well plate (655090, Greiner) and polymerized for 1 hour at 37 °C.
-
bioRxiv - Biochemistry 2020Quote: ... The eluted phage solution was transferred to a 96 deep well plate (Greiner Bio-One, Frickenhausen, Germany) and incubated with 150 μL E ...
-
bioRxiv - Cancer Biology 2023Quote: ... and loaded the solution (25 µL per well) into a high-binding 384-well plate (Greiner, 781077). The plate was placed on an orbital shaker ...
-
bioRxiv - Immunology 2024Quote: ... hearts were mounted in Ce3D clearing solution in 35 mm glass bottom CELLview culture dishes (Greiner; 627861).
-
bioRxiv - Cell Biology 2020Quote: ... diluted 1:1000 in 400 µl of antibody solution (2.5% BSA, 0.05% Triton X-100, 1×PBS) in a 12-well culture plate (Greiner) and incubated overnight for at least 15 h at RT ...
-
bioRxiv - Systems Biology 2020Quote: ... 180 µL of each culture solution was transferred to Greiner 96-well microwell white plate (Greiner, Frickenhausen, Germany) and pre-incubated with shaking at 37°C for 15 min ...
-
bioRxiv - Genomics 2021Quote: ... in Hanks Balanced Salt Solution without Mg2+/Ca2+ and filtered through a 70-μmcell strainer (Greiner Bio-One). Cells were pelleted at 2000g 5min room temperature and washed twice with 10% Fetal Bovine Serum (Thermofisher ...
-
bioRxiv - Microbiology 2024Quote: ... electroporated cells were resuspended in 50% matrigel-ODM solution and plated in black wall 96-well plates (Greiner) pre-coated with 5% matrigel ...
-
bioRxiv - Neuroscience 2023Quote: ... Drug compound solutions were prepared in assay buffer in clear V-bottom 96-well plates (GR-651161, Greiner). Typically ...
-
bioRxiv - Biophysics 2024Quote: ... Culture solutions (50 mL) were dispensed into dark well tissue culture plates (Greiner Bio-One CELLSTAR, 384-well) along with BCM (when used ...
-
bioRxiv - Immunology 2020Quote: ... and 80 U/ml DNase (Life Tech) in Hank’s balanced-salt solution before forcing tissue suspensions through a cell strainer (70 μm, Greiner). Red blood cells were lysed using Red Blood Cell Lysing Buffer Hybri Max (Sigma ...
-
bioRxiv - Biochemistry 2022Quote: ... and a 200 μL aliquot of each solution was transferred to a 96-well UV-Star microtiter plate (Greiner) for analysis with a microplate reader (Spark ...
-
bioRxiv - Molecular Biology 2024Quote: ... These protein solutions were then dispensed in triplicate into a clear-bottom 384-well microplate (Greiner Bio-One, Austria) to a final volume of 50 µL per well ...
-
bioRxiv - Microbiology 2024Quote: ... 100μl of each sample was supplemented with 5µM 11S and 2µl furimazine (1:100 dilution of stock solution) in a 96-well plate (Greiner, REF655073) with an approximate final well volume of 105μl ...
-
bioRxiv - Biophysics 2024Quote: ... Compounds 1-4 were diluted to 50x in DMSO and serially diluted 1:2 six times and 3.1 µL of each solution was added to 150 µL of SPR buffer in a 384-well plate (Greiner). NADH was dissolved in SPR buffer with 2% DMSO at 50X and diluted 1:2 six times in 150 uL of SPR buffer with 2% DMSO ...
-
bioRxiv - Immunology 2022Quote: ... 200 nl of the small molecule stock solution (10 mM in DMSO) were transferred to white 384-well microplates (Greiner) using an Echo 555 Acoustic Technology Liquid Handler (Labcyte) ...
-
bioRxiv - Microbiology 2020Quote: ... Caspase 3/7 activity was measured at each time point by incubating cells in equal amounts of Caspase Glo solution and cell media for 30 min at RT and reading luminescence in a white 96-well plate (Greiner) with a Synergy HT or Synergy H1 plate reader (Biotek).
-
bioRxiv - Microbiology 2022Quote: ... A 200μL aliquot of inoculated organic acid solution for each strain was transferred to a 96-well U-Bottom plate (Greiner), in triplicate wells ...
-
bioRxiv - Synthetic Biology 2022Quote: ... CF-TXTL was conducted using 15 μL reaction solutions at 37 °C in a 96-well plate (polystyrene, solid bottom, half area, black-walled, Greiner Bio-One International GmbH ...
-
bioRxiv - Microbiology 2023Quote: ... 120 μL of the pre-cooled nitrocefin solutions were mixed inside pre-cooled clear 96-well plate (Greiner F-bottom) with 60 μL of pre-incubated PSMα solution (510 μM PSMα ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 700 µL of 2.15 × concentrated solutions of assembled DNA nanostructures were placed in 50 ml centrifuge tubes (Greiner Bio-One) and supplemented with 700 µL of PB(2 ×)M9 ...
-
bioRxiv - Biochemistry 2024Quote: ... 80 µl of the assayed solution were transferred to a transparent 96-well flat-bottom plate (Greiner Bio-One, Germany). Kd and IC50 determinations for compounds 20 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and fixed with 8% formaldehyde and 0.25% glutaraldehyde solution (1:1 v/v) for 30 minutes in Cellview cell culture slides (PS, 75/25 mm, glass bottom; Greiner). The fixed samples were washed with MilliQ water and covered with Vectashield Antifade Mounting Medium (Vector Laboratories) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The reactions were incubated for 1 hour at 37 °C and the 35 µl of solutions were transferred to a 384-well clear plate (Greiner). 35µl of 5mg/ml X-Gal ...
-
bioRxiv - Biochemistry 2024Quote: ... Typically 100 nL of the GP1-GP2e-Fab complex (∼5mg/ml) was combined with 100 nL of reservoir solution in 96-well sitting drop plates (Greiner), and the mixture was allowed to equilibrate against 90 uL of reservoir solution (62) ...
-
bioRxiv - Biophysics 2021Quote: ... Sample solutions (200 μl) were applied in triplicate to each well of the 96-well microplate (Greiner-Bio-One, Tokyo, Japan), and sealed with a film (PowerSeal CRISTAl VIEW ...
-
bioRxiv - Molecular Biology 2020Quote: ... Compound solution and Boc-Gln-Ala-Arg-MCA (10 μM final concentration) were added into the 384-well black plate (Greiner 784076). Then ...
-
bioRxiv - Genetics 2022Quote: ... brains were dissected in ice cold Ca2+ free Ringer’s solution and placed on a CellView glass bottom imaging dish (35x10mm; Greiner Bio One) treated with Heptane glue ...
-
bioRxiv - Biophysics 2023Quote: ... 35.000 cells are mixed with 3 ml collagen solution (final collagen concentration of 1.2 mg/ml) and transferred to a 35 mm Petri dish (Greiner AG, Austria) and polymerized at 37° for 60 minutes ...
-
bioRxiv - Biochemistry 2021Quote: ... Sitting drops were set up using 400 nL of a 1:1 mixture of each sample protein and crystallization solutions (672 different commercially available conditions) equilibrated against a 150 μL reservoir in multiwell plates (Greiner Bio-One). The crystallization plates were stored at 4 °C in a RockImager1000® (Formulatrix ...
-
bioRxiv - Immunology 2021Quote: ... 1 µL of DMSO solutions was spiked into dry well of F-bottom clear cell culture treated 96-wells plates (Greiner Bio One), with columns 1 and 12 devoted to controls and used to calculate the Z’-factor ...
-
bioRxiv - Developmental Biology 2023Quote: ... a handmade device was created: 1.5 % agarose solution was added to glass-bottomed dishes (CELLview™, #627860, Greiner Bio-One, Kremsmünster, Austria), and a 7-unified cover-glass (18 mm x 18 mm with 0.12-0.17 mm thickness ...
-
bioRxiv - Genetics 2022Quote: ... Transfected cells were washed twice with 2 mL of extracellular solution (ECS, see below) and re-plated onto 35-mm polystyrene cell culture dishes (Cellstar, Greiner Bio-One). hKV1.3 currents were recorded from activated peripheral blood mononuclear cells (PBMCs ...
-
bioRxiv - Cancer Biology 2023Quote: Heparinized blood from patients was diluted with phosphate-buffered saline (PBS) and layered onto Biocoll Separation Solution (Biochrom) in Leucosep tubes (Greiner Bio-One) followed by a density-gradient centrifugation (800g without brake at room temperature) ...
-
bioRxiv - Biochemistry 2024Quote: ... 100 nL of compound stock solutions were dispensed into white CELLSTAR® 96-well plates with tissue culture-treated surface (Greiner, 655083). Similarly ...
-
bioRxiv - Genetics 2020Quote: ... The plate was incubated in the dark for 30 min on a plate rocker at room temperature and 100 μL of the solution was transferred to a flat bottom white wall 96-well plate (Greiner Bio-One, Austria). The luminescence was measured using a Cytation3 imaging reader and the Gen5 software V3.03 (BioTek ...
-
bioRxiv - Biophysics 2022Quote: ... 0.83 μL of 30 mg/mL lysozyme solution was mixed with the equal volume of ∼3000 commercially available sparse matrix and grid screen crystallization cocktail solutions in 72-well Terasaki crystallization plates (Cat#654180, Greiner Bio-One, Austria). Each well was sealed with 16.6 μL of paraffin oil (Cat#ZS.100510.5000 ...
-
bioRxiv - Biophysics 2023Quote: ... Equal volumes of crystallization conditions were mixed with 0.83 μL of 30 mg/mL lysozyme solution (1:1 v/v) in a 72-well Terasaki plate (Cat#654180, Greiner Bio-One, Austria). Then ...
-
bioRxiv - Microbiology 2024Quote: ... 100 µL of the filtered supernatant was mixed with 100 µL of CAS solution in a well of a 96-well microplate with flat bottoms (Greiner Bio-One #655161). Controls included uninoculated media mixed with CAS solution and an iron-free variant of the CAS solution (APO-CAS) ...