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Citations for Greiner :
51 - 100 of 129 citations for Lysozyme C LYZ Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Bio-layer interferometry (BLI) was performed at 25°C in 96-well black flat bottom plates (Greiner) using an acquisition rate of 5 Hz averaged by 20 using an Octet© R8 BLI System (FortéBio/Sartorius) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were grown in a humidified atmosphere with 5% CO2 at 37 °C in T175 flasks (Greiner). Cells were split twice a week by washing with Dulbecco’s phosphate buffered saline (DPBS ...
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bioRxiv - Immunology 2022Quote: ... Anti-Human Fc biosensors (ForteBio, catalog #18-0015) were submerged in wells of black 96-well microplates (Greiner, catalog #655209) containing 250 μL of kinetics buffer (PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... 5×103 human SCLC cells were seeded in a 96-well cell culture plate (Cat no.: 655180; Greiner Bio-One). After allowing cells to adhere during a 4h incubation at 37°C ...
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bioRxiv - Biochemistry 2021Quote: Human osteosarcoma U2OS cells stably expressing a fusion protein of GFP with human parkin (https://www.addgene.org/45875/) were seeded on a 35-mm Glass Bottom 4 Compartment Dish (Greiner Bio-One) (Tang et al ...
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bioRxiv - Microbiology 2022Quote: 25 μl of ferret sera or human mAbs were two-fold serially diluted in 96-well V-bottom plates (Greiner) with PBS prior to being mixed with equal volume of H7N9 Anhui/13 (8 HA units) ...
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bioRxiv - Neuroscience 2022Quote: ... Thirty microliters of 1 μM human PrP23–111 (10 mM sodium carbonate, pH 9.6) was bound to high binding 384-well white plates (Greiner #G781074) with shaking at 400 RPM for 1 h at 37 °C ...
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bioRxiv - Genetics 2021Quote: All hESC lines were maintained at 37°C and 5% CO2 in 6 well cell culture plates (Greiner) coated with 1% Matrigel hESC-Qualified Matrix (Corning ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Samples were preincubated for 30 min at 37°C and pipetted in triplicate into 384-well plates (Greiner). Thereafter ...
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bioRxiv - Microbiology 2023Quote: Biofilms formed under static conditions at 37°C for 24 h in cell culture 24-well plates (Greiner) were stained with safranin as described elsewhere57 ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were maintained at 37 °C and 5 % CO2 in cell culture flasks with filter lids (Greiner). The cells were split at regular intervals to keep the population density below 1×106 cells/ml ...
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bioRxiv - Microbiology 2023Quote: ... and stored with 25% glycerol at –80 °C in 96-well flat-bottom plates (Greiner Bio-One #655161).
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bioRxiv - Cancer Biology 2020Quote: ... A fresh frozen human brain tumor tissue piece (20-50 mg) was placed on a Petri dish (Greiner Bio-One 628160) on a cooled metal block ...
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bioRxiv - Neuroscience 2019Quote: ... and αSyn-Q-(Position#)C-Cy5 (acceptor) in PBS (pH 7.4) were dispensed into black/clear bottom 384-well plates (Greiner) using a Mantis liquid handler (Formulatrix) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Individual Gα reporter strains were grown in SCD LoFo pH = 7.0 at 30 °C to an OD = 1.0 in a 2.0 mL 96-well DeepWell block (Greiner; 780271-FD). Cells were normalized to an OD of 0.1 in SCD LoFo pH = 7.0 using a Biomek NXp liquid-handling robot ...
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bioRxiv - Immunology 2022Quote: ... and 1:750 for Fcγ-receptor binding) overnight at 4°C in 384 well plates (Greiner Bio-One, Germany). Secondary antibodies were PE-conjugated and incubated with samples at room temperature for 1 hour at a 1:100 dilution in sterile-filtered Assay Buffer (1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... for 90 minutes at 37°C with agitation at 300 rpm in sterile round-bottom 96-well plates (Greiner Bio-One ...
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bioRxiv - Microbiology 2022Quote: ... Cells were maintained at 37°C and 5.0% CO2 in 10 cm cell culture dishes (Greiner Bio-one, 664160).
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bioRxiv - Immunology 2022Quote: ... and 1:750 for Fcγ-receptor binding) overnight at 4°C in 384 well plates (Greiner Bio-One, Germany). Unbound antibodies were removed by washing and subclasses ...
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bioRxiv - Biophysics 2023Quote: ... Batch C) are mixed with 35 000 cells and transferred to a 35 mm Petri dish (Greiner AG, Austria). Samples are polymerized at 37°C for 60 minutes ...
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bioRxiv - Biophysics 2020Quote: Human umbilical vein endothelial cells (HUVECs) are seeded overnight on a 35 mm diameter glass bottom dish (Greiner Bio CELLVIEW Cell Culture Dish) until they form a confluent monolayer ...
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bioRxiv - Bioengineering 2023Quote: ... human brain-like ECs were grown in 50% PC-conditioned medium on 35 mm glass bottom Petri dishes (Greiner Bio-One, cat# 627960) coated with collagen IV (100 µg/mL ...
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bioRxiv - Bioengineering 2023Quote: Human brain-like ECs were cultured in 50% PC-conditioned medium on 35 mm glass bottom Petri dishes (Greiner Bio-One, cat# 627860) coated with collagen IV (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... and 1:100 for all other readouts) for 2 hours at 37°C in 384-well plates (Greiner Bio-One). The high affinity FcR was not tested due to its minimal role in tuning antibody effector function 48 ...
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bioRxiv - Biochemistry 2020Quote: ... All steps were performed at 30°C with shaking at 1000 rpm in a black 96-well plate (Greiner 655209), with a working volume of 200 μL in each well ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4°C overnight in distilled water prewashed (to remove anticoagulant) vacutainer tubes (Vacutainer, #454087, Greiner GmbH, Austria). The next day ...
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bioRxiv - Bioengineering 2021Quote: ... The collected cells were blocked in 2% BSA in PBS for 30 min at 4°C and then transferred to a 96 well V-shape microplate (Greiner) at a density of 100,000 per well ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4°C overnight in distilled water prewashed (to remove anticoagulant) vacutainer tubes (Vacutainer, #454087, Greiner GmbH, Austria). The next day ...
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bioRxiv - Immunology 2022Quote: ... and 1:100 for all other readouts) for 2 hours at 37°C in 384-well plates (Greiner Bio-One). Unbound antibodies were washed away ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Suspensions of 1.5 × 106 cells/ml were cultivated at 37°C with 7.5% of CO2 in Petri dishes (Petri Greiner ref: 664161) using complete DMEM medium (Pan DMEM (Biotech P04-03588 ...
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bioRxiv - Systems Biology 2019Quote: ... Figure S3B-C) separate 50-mL tubes with a ventilated cap (CELLSTAR CELLreactor filter top tube, Greiner Bio-One, 227245), each containing 35 mL of yeast culture ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The venom and inhibitors were preincubated for 30 min at 37 °C and pipetted in triplicate onto 384-well plates (Greiner). A Labsystems Multidrop Reagent dispenser was used to dispense the substrate ...
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bioRxiv - Developmental Biology 2019Quote: ... experiments and snapshot images of whole spinal cord were carried out using a Zeiss LSM880 microscope with a C-Apochromat 40x 1.2 NA water objective on slices placed directly on a glass-bottomed dish (Greiner BioOne) kept at 37°C and 5%CO2 ...
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bioRxiv - Evolutionary Biology 2020Quote: Overnight cultures were diluted 1:103 in fresh competence medium and grown for 4.5 h (37°C, 250 rpm) in a 24-well microtiter plate (1 mL, final OD ≈ 0.25; Greiner Bio-one). Microtiter plates were covered with rayon film adhesive covers (VWR) ...
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bioRxiv - Physiology 2021Quote: ... and samples were incubated for 30 minutes at 37°C with gentle shaking in 96-well Microplates (Greiner Bio-One). Glucose was measured by mixing 10 ul of homogenate in 100ul of Infinity Glucose Hexokinase Reagent (Thermo Fisher Scientific™ - TR15421) ...
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bioRxiv - Physiology 2021Quote: ... and samples were incubated for 30 minutes at 37°C in 96-well Microplates UV-Star (Greiner Bio-One – 655801) with gentle shaking ...
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bioRxiv - Immunology 2021Quote: ... and 1:100 for all other readouts) for 2 hours at 37°C in 384-well plates (Greiner Bio-One). Unbound antibodies were washed away ...
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bioRxiv - Microbiology 2020Quote: ... were inoculated from −80 °C freezer stocks into 200 µL of the medium of interest in shallow 96-well plates (Greiner) and grown overnight for 18 h while shaking at 37° C ...
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bioRxiv - Synthetic Biology 2022Quote: ... CF-TXTL was conducted using 15 μL reaction solutions at 37 °C in a 96-well plate (polystyrene, solid bottom, half area, black-walled, Greiner Bio-One International GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... 20 μmol/L unphosphorylated and phosphorylated C1mC2 fragments were incubated with 20-fold SYPRO Orange for 10 min at 25°C in black 96-well plates (Greiner), and SYPRO Orange fluorescence measured using appropriate excitation and emission filter settings using a ClarioStar plate reader (BMG Labtech).
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bioRxiv - Biochemistry 2023Quote: CatL activity at a concentration of 5 nM in the presence of seven different concentrations of the inhibitors and in the absence of inhibitor (control sample) was monitored in real time for 30 min at 37 °C in 96-well black flat bottom microplates (Greiner) using an INFINITE M1000 pro plate reader (Tecan) ...
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bioRxiv - Microbiology 2023Quote: 3 mL cultures of ΔmxaF/pAZ1 were grown in MP medium supplemented with 15 mM succinate and 50 μg/mL kanamycin overnight at 29 °C and 200 rpm in 14 mL vented cap culture tubes (Greiner). Multiple cultures were inoculated into 250 mL of MP medium supplemented with 15 mM succinate and 50 μg/mL kanamycin within a 500 mL Ultra Yield flask (Thompson Instrument ...
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bioRxiv - Microbiology 2023Quote: ... LUVs were incubated at 0.4 mM lipid concentration in 10 mM sodium acetate (pH 5) at 25°C in a black p96-well plaque (Greiner), and fluorescence titrations were performed with an FP-8200 Jasco spectrofluorometer equipped with a Peltier-thermostated ETC-272T (25°C) ...
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bioRxiv - Molecular Biology 2024Quote: ... and the emission spectra were recorded at 25°C from 300 to 500 in a 96-well black bottom microplate (Greiner). A bandwidth of 7.5 nm was used for the excitation and emission beams ...
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bioRxiv - Immunology 2024Quote: BLI competition assays were performed using an Octet RED384 instrument at 30°C in a 96-well polypropylene black 96-well microplate (Greiner). Antibody competition assays were done using an in-tandem assay ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were cultured at 30°C with 200 RPM shaking in 50 mL CELLreactor™ filter top tubes (Greiner Bio-On). Strains were grown overnight in 5 mL synthetic maltose media lacking uracil ...
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bioRxiv - Systems Biology 2019Quote: Bone marrow cell suspensions were recovered from tibias and femurs of BALB/c mice (Janvier) and suspended in bacteriologic Petri dishes (Greiner, Germany) at a concentration of 1.35×106 ml−1 in complete medium i.e ...
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bioRxiv - Cancer Biology 2019Quote: ... Assays were carried out after 10 min incubation at 37°C in black half area 96-well plates (Greiner Bio-One) in 0.15 M NaCl ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cultures were then inoculated into fresh media supplemented with different glycans and cell membrane treated glycan media to an initial OD600 of 0.05 and incubated at 37 °C without shaking in a 96-well plate (Greiner Bio-One). Cell growth was monitored by plate reader (Tecan F200).
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bioRxiv - Synthetic Biology 2021Quote: ... The cultures were inoculated into both fresh media and conditioned media with an initial OD600 of 0.05 and anaerobically grown at 37 °C in a 96-well plate (Greiner Bio-One) without shaking ...