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Citations for Greiner :
101 - 150 of 336 citations for 8 Chloro 2 methylimidazol 1 2 a pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Caco-2 cells were seeded on the upper side of ThinCert membrane inserts (pore size, 3 μm) (Greiner Bio-One, Germany) in a 24-well plate overnight ...
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bioRxiv - Developmental Biology 2019Quote: ... Multi-mounts (≥ 2 embryos/well) were prepared with 300 μl ERM/E3 per well using flat (F) bottom 96-well plates (Greiner, 655101).
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bioRxiv - Cell Biology 2020Quote: ... or 3 μL of pelleted fraction 2-9 (duplicates) were added into a 96-well white plate (Greiner Bio-One, Germany). Furimazine (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... Approximately 350,000 cells were cultured in 2 ml of culture medium in a well (6-well plates were used, Greiner, Nurtinger, Germany) and incubated for 24 h with different doses of AKOS004122375 in the absence of fetal calf serum ...
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bioRxiv - Microbiology 2023Quote: Forty microliters of thawed cryo-stocks were transferred from the 96-microwell plates of the progenitor collection to the condensed collection in 2-mL 96-deepwell plates (Greiner, 780270) previously inoculated with 1 mL of commercial MRS with 10 µg/mL tetracycline and pre-warmed at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... supernatants were removed from Caco-2-ACE2 and VeroE6-TMPRSS2 cells seeded previously in µClear 96 well microplates (Greiner Bio-One) and replaced with 100 µl of the virus:sera dilutions followed by incubation for 18 hours at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... with 2% (vol/vol) heat-inactivated FBS) and flushed through a 40 µm mesh cell strainer (Greiner Bio-One, Monroe, NC). GFP-expressing cells were sorted from non-transfected cells on a Becton Dickinson (BD ...
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bioRxiv - Microbiology 2024Quote: ... Plate was then incubated at 37°C + 5% CO2 for 2 hours before 60% of the wells’ supernatant was carefully transferred into a black-well plate (Greiner, #655076). A VarioskanTM LUX plate reader (Thermo Fisher Scientific #VL0000D0 ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... Samples were taken every hour for 8 hours and diluted to an OD600 of approximately 0.03 in phosphate-buffered saline (PBS) with 2 mg/mL of kanamycin sulfate in round bottom plates (Greiner Bio-One #650101). Samples were run on an Attune NxT Flow Cytometer via the autosampler on the same day and at least 20,000 events per sample were captured ...
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bioRxiv - Biophysics 2020Quote: ... cells were collected by centrifugation at 114 RCF for 5 min and transferred into 2 ml of fresh medium in a cell culture-treated 6-well plate (#657160, Greiner Bio-One) to provide for sufficient space for further growth.
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bioRxiv - Bioengineering 2021Quote: ... 2 μM of CDA5 or CDA5-Y34A was incubated in black flat bottom half volume opaque 96-well plates (Greiner Bio-One). For nucleotide response assays ...
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bioRxiv - Microbiology 2021Quote: ... ACE-2 transduced H1299-H2AZ cells were then sub-cloned at the single cell density in 384-well plates (Greiner Bio-One) in conditioned media derived from H1299 confluent cells ...
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bioRxiv - Molecular Biology 2021Quote: ... spiked controls and diluted samples were mixed with 3 nM VHH72-h1 (S56A)-Flag3-His3 and 2.5 nM biotinylated SARS-CoV-2 RBD protein in white low binding 384-well microtitre plates (F-bottom, Greiner Cat nr 781904). After an incubation for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... were produced by inoculation of VeroE6/TMPRSS2 cells and titred as previously described.(33, 36) Caco-2-ACE2 cells and cardiac PCs were plated in μClear 96-well microplates (Greiner Bio-one) and the next day infected with either REMRQ0001 or B.1.1.7 at multiplicity of infection (MOI ...
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bioRxiv - Immunology 2023Quote: HMVEC were seeded at 5 × 103 cells/well in EBM-2 cell culture medium in a flat bottom 96-well plate (Greiner Bio-One). After overnight settling of the cells ...
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bioRxiv - Genetics 2022Quote: ... Transfected cells were washed twice with 2 mL of extracellular solution (ECS, see below) and re-plated onto 35-mm polystyrene cell culture dishes (Cellstar, Greiner Bio-One). hKV1.3 currents were recorded from activated peripheral blood mononuclear cells (PBMCs ...
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bioRxiv - Cell Biology 2022Quote: hPOs were seeded in 5 µL BME-2 droplets in sterile CELLSTAR® 96-well suspension culture plates (Greiner Bio-One GmbH) and overlaid with 100 µL hPO medium ...
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bioRxiv - Cell Biology 2020Quote: ... was allowed to polymerize in 8 µm inserts (662638; Greiner Bio-One) for 1 h at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... in 50 μl of DMEM containing 2% FBS were seeded in each well of black μCLEAR flat-bottom 96-well plate (Greiner Bio-one™). The cells were incubated overnight at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μL of two-fold serial dilutions of PdPV1 in PBS were incubated with an equal volume of 2% (v/v) erythrocytes in PBS in U-shaped microtiter plates (Greiner Bio One, Germany) at 37 °C for 2 h ...
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bioRxiv - Biochemistry 2021Quote: 50 µL of each purified soluble RBD variant at a protein concentration of 2 µg/ml was immobilized to Microlon clear plates (Greiner Bio-One 655081) at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: HEp-2 cells conjugated to NanoShuttlesTM were seeded on a 24-well ultralow-attachment plate (ULA, Cellstar® Greiner Bio-one, Kremsmünster, Austria) in 105 cells and a final volume of 400 μL/well.
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bioRxiv - Cancer Biology 2022Quote: Cells were plated at 300,000 cells/mL in RPMI medium with 10% serum at 2 mL per well in 6-well treated tissue culture plates (Greiner, Cat. # TCG-657160) to achieve 75% density the following day and incubated overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... T47D) in RPMI medium with 10% serum at 2 mL per well in 6-well treated tissue culture plates (Greiner, Cat. # TCG-657160) and incubated overnight ...
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bioRxiv - Microbiology 2023Quote: ... at each time point 2 x 200 µL of each culture were collected into a sterile 96-well plate (F-bottom) (Greiner Bio-One Cellstar) and then frozen at −80 °C ...
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bioRxiv - Immunology 2023Quote: ... 1,000,000 cells in 2 ml culturing media were used for the 25 mm diameter coverslips (in a 6-well cell culture plate Greiner Bio-One #657160) and 200,000 cells in 200 µl culturing media were added to the apical compartment of transwell (the basolateral compartment of transwells was filled with 600 µl culturing media) ...
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bioRxiv - Microbiology 2023Quote: ... was incubated with 50µL of magnetic beads coated with recombinant protein (2 µg protein/1.25 × 106 beads) in 96-well flat-bottom chimney plates (Greiner bio one, Frickenhausen, Germany) on a plate shaker at 300 rpm for 16 h at 4 °C in the dark ...
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bioRxiv - Immunology 2023Quote: ... Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One, 675061) as previously described (Hernández-Pérez et al. ...
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bioRxiv - Genetics 2023Quote: ... at 0.33 x 107 cells per well for the RNA sequencing experiment and 2 x 105 cells per well in a 96-well (M0562, Greiner, Sigma Aldrich, USA) for microscopy ...
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bioRxiv - Microbiology 2023Quote: ... was diluted to 100-fold the working concentration in DMSO (2 mM) in V-bottom polypropylene plates (Greiner Bio-One, cat. No. 651261). For the screening experiments ...
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bioRxiv - Microbiology 2023Quote: ... were diluted in DMEM and thirty microliters of each tested concentration were manually dispensed into a well (with 2-3 replicates for each concentration) of a 96-well white plate (Greiner Bio-One, NC). Thirty microliters of viruses pseudotyped with specific Envs were then added ...
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bioRxiv - Microbiology 2023Quote: ... 1- or 2-μl droplets were deposited in the wells of a 96-well plate with a non-binding surface (Greiner Bio-One, 655901), one droplet per well ...
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bioRxiv - Genomics 2022Quote: ... Vacuette Tube 8 ml K2E K2EDTA Separator (EDTA separator; Greiner Bio-One, 455040), BD Vacutainer Glass ACD Solution A tube (ACD-A ...
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bioRxiv - Cancer Biology 2023Quote: 24 well transwell with 8 μm pore membrane (Greiner bio-one, catalog: 662638) was coated with 10 μg/ml fibronectin in sterile DI water solution and incubated at 37° C for 2 hours ...
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bioRxiv - Biophysics 2020Quote: ... 0.5 μM (gPRM)14 and 1 μM mMDM2 in KMEI buffer supplemented with 2% DMSO in a total volume of 20 μL in a 384-well microplate (Greiner Bio-One, Cat. 781090). High-throughput screening was performed using compounds in a commercial library (Selleck-2148) ...
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bioRxiv - Cancer Biology 2022Quote: ... After 96 hours, tumor organoids were shock-frozen in precooled 2-methylbutan (Carl Roth, Karlsruhe, Germany) using a magnetic pen (Greiner Bio-One, Kremsmünster, Austria) to transfer them into cryovials.
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bioRxiv - Genomics 2021Quote: ... The cells were plated at a density of 2 × 106 cells per 75 cm2 tissue culture flasks (CELLSTAR® Greiner Bio-One GmbH, Frickenhausen, Germany) in Minimum Essential Medium Eagle culture medium (MEM ...
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bioRxiv - Bioengineering 2021Quote: ... of CCB cells seeded in DMEM with a density of 60 000 cells cm-2 in a cell disc (1000 cm2, Greiner Bio-One GmbH, Kremsmünster, Germany). Infected cells were incubated at 25 °C for three days post infection (p.i. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The SARS-CoV-2 3CLpro assay was initiated by dispensing 2 µL/well of 50 nM enzyme solution into 1536 black bottom microplates (Greiner Bio-One, Monroe, NC, USA) by a Multidrop Combi disperser (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: 333 microliters of pre-reduced and filter-sterilized BHIS (with no added cysteine) were added to 2-mL 96-deep well plates (Greiner Bio-One, Cat. #1986-2110) using a semi-automated BenchSmart pipettor (Mettler-Toledo ...
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bioRxiv - Cell Biology 2022Quote: ... Organoids were sheared into fragments prior to resuspension in ice-cold liquified BME-2 and seeded as droplets in pre-warmed non-treated CELLSTAR® 48-well suspension culture plates (Greiner Bio-One GmbH, Kremsmünster, AUT). Organoid-containing BME-2 droplets were polymerized for 15 min at 37°C after which the droplets were overlaid with hPO medium.
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bioRxiv - Cancer Biology 2021Quote: ... on top of a Transwell insert with 8 μm pores (Greiner Bio-One, Kremsmunster, Austria). A serum gradient was applied and cancer cells were allowed to migrate for four days ...
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bioRxiv - Cell Biology 2020Quote: Cells were seeded at 8×104 cells/ml in 96 well plates (Greiner Bio-One), 24 hours prior to drug treatment ...
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bioRxiv - Microbiology 2020Quote: ... 1L×L105 cells were gently added to the transwell insert (8 μm, Greiner Bio-One). DMEM with 10% FBS was added to the bottom of the lower chamber (24-well plate) ...
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bioRxiv - Cancer Biology 2021Quote: Transwell migration assays were performed in ThinCert 24-well transwell inserts (8 μm pore size, 662638, Greiner). 2.5×105 691-MES or 691-ADRN or 1×105 717-MES or 717-ADRN cells were allowed to migrate for 48 hours through a transwell to a gradient of B27 (20% B27 in the upper chamber of transwell to 100% B27 as chemoattractant in the lower chamber of 24-well plate ...
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bioRxiv - Cell Biology 2020Quote: hTCEpi cells were seeded at 8 x 105 cells per dish in 60 mm dishes (Greiner-BioOne) and cultured in 2 mL BPE-free KSFM media for 48 h ...
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bioRxiv - Developmental Biology 2020Quote: ... 2.0 × 104 HTR8 EVTs were placed into transwell inserts (8-μm pore, 6.5-mm diameter, Greiner BioOne). Media containing 3.8 × 104 human uterine microvascular endothelial cells ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... 4T1) were plated in the upper chamber of an 8 μm pore-size ThinCert (Greiner Bio-One) in serum-free media 24 hours after ASO or siRNA transfection ...
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bioRxiv - Cell Biology 2020Quote: 8 x 103 cells were plated per well in a black μClear 96-well plate (Greiner Bio-One) and transfected as described above ...
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bioRxiv - Cell Biology 2020Quote: ... 8 x 103 U2OS cells per well were seeded in a Cellstar 96-well plate (Greiner Bio-One) and transiently transfected with cDNAs encoding GFP-actin ...