Labshake search
Citations for Greiner :
351 - 400 of 549 citations for 7 trifluoromethyl 1 2 3 4 tetrahydro 5 nitroisoquinoline hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: 12 two-fold serial dilutions of nitroxoline and 32 other compounds (Supplementary Table 2) were arrayed in technical duplicates in 384-well plates (ref. 781271 by Greiner BioOne) and inoculated with E ...
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bioRxiv - Biochemistry 2019Quote: ... The pre-diluted plasma samples and the pre-diluted protein standards (45 μl) were transferred to 5 μl bead stock in an assay plate (Greiner 384-well assay plate) using a liquid handler (SELMA ...
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bioRxiv - Microbiology 2021Quote: ... were collected from the facial vein using a sterile 5 mm goldenrod lancet (MEDIpoint, Mineola, NY) into collection tubes containing lithium heparin (Greiner Bio-One, Kremsmünster, Austria). Plasma was separated by centrifugation of blood samples at 1,500 × g for 15 min at 4°C and stored immediately at −80°C until analysis ...
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bioRxiv - Cell Biology 2021Quote: ... The stroma was cut in 9 × 7 mm pieces for the dermis and 5 × 10 mm pieces for the submucosa and placed onto transparent ThinCertTM inserts of 0.4 μm pore size (Greiner Bio-One Ltd; Cat#657641). Immediately afterwards ...
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bioRxiv - Immunology 2023Quote: ... Catalase activity was measured by adding 5 uL of sample protein extract to a 96-well UV well plate (UV-STAR, Greiner bio-one, Frickenhausen, Germany), which was diluted with 45 uL of 50 mM PBS (pH 7.0) ...
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bioRxiv - Microbiology 2023Quote: ... and a suspension containing 0.5 × 109 cells [26] in 5 ml of mTSB was transferred into a 50-ml cell culture flask (Cellstar® #690160, Greiner Bio-One, Germany). The cells were cultured for 20 h to allow them to attach to the bottom of the flask and form a biofilm ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were isolated with NP40 lysis buffer with a 5 min incubation on ice and passed through a 70 μm cell strainer (Greiner Bio-One EASYstrainer, ThermoFisher). Samples were pooled based on nuclei concentration (Countess II cell counter ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... Samples were taken every hour for 8 hours and diluted to an OD600 of approximately 0.03 in phosphate-buffered saline (PBS) with 2 mg/mL of kanamycin sulfate in round bottom plates (Greiner Bio-One #650101). Samples were run on an Attune NxT Flow Cytometer via the autosampler on the same day and at least 20,000 events per sample were captured ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μM of CDA5 or CDA5-Y34A was incubated in black flat bottom half volume opaque 96-well plates (Greiner Bio-One). For nucleotide response assays ...
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bioRxiv - Microbiology 2021Quote: ... ACE-2 transduced H1299-H2AZ cells were then sub-cloned at the single cell density in 384-well plates (Greiner Bio-One) in conditioned media derived from H1299 confluent cells ...
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bioRxiv - Molecular Biology 2021Quote: ... spiked controls and diluted samples were mixed with 3 nM VHH72-h1 (S56A)-Flag3-His3 and 2.5 nM biotinylated SARS-CoV-2 RBD protein in white low binding 384-well microtitre plates (F-bottom, Greiner Cat nr 781904). After an incubation for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... were produced by inoculation of VeroE6/TMPRSS2 cells and titred as previously described.(33, 36) Caco-2-ACE2 cells and cardiac PCs were plated in μClear 96-well microplates (Greiner Bio-one) and the next day infected with either REMRQ0001 or B.1.1.7 at multiplicity of infection (MOI ...
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bioRxiv - Genetics 2022Quote: ... Transfected cells were washed twice with 2 mL of extracellular solution (ECS, see below) and re-plated onto 35-mm polystyrene cell culture dishes (Cellstar, Greiner Bio-One). hKV1.3 currents were recorded from activated peripheral blood mononuclear cells (PBMCs ...
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bioRxiv - Immunology 2021Quote: ... in 50 μl of DMEM containing 2% FBS were seeded in each well of black μCLEAR flat-bottom 96-well plate (Greiner Bio-one™). The cells were incubated overnight at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μL of two-fold serial dilutions of PdPV1 in PBS were incubated with an equal volume of 2% (v/v) erythrocytes in PBS in U-shaped microtiter plates (Greiner Bio One, Germany) at 37 °C for 2 h ...
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bioRxiv - Biochemistry 2021Quote: 50 µL of each purified soluble RBD variant at a protein concentration of 2 µg/ml was immobilized to Microlon clear plates (Greiner Bio-One 655081) at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: HEp-2 cells conjugated to NanoShuttlesTM were seeded on a 24-well ultralow-attachment plate (ULA, Cellstar® Greiner Bio-one, Kremsmünster, Austria) in 105 cells and a final volume of 400 μL/well.
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bioRxiv - Cancer Biology 2022Quote: Cells were plated at 300,000 cells/mL in RPMI medium with 10% serum at 2 mL per well in 6-well treated tissue culture plates (Greiner, Cat. # TCG-657160) to achieve 75% density the following day and incubated overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... T47D) in RPMI medium with 10% serum at 2 mL per well in 6-well treated tissue culture plates (Greiner, Cat. # TCG-657160) and incubated overnight ...
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bioRxiv - Microbiology 2023Quote: ... 1- or 2-μl droplets were deposited in the wells of a 96-well plate with a non-binding surface (Greiner Bio-One, 655901), one droplet per well ...
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bioRxiv - Microbiology 2023Quote: ... was diluted to 100-fold the working concentration in DMSO (2 mM) in V-bottom polypropylene plates (Greiner Bio-One, cat. No. 651261). For the screening experiments ...
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bioRxiv - Microbiology 2023Quote: ... at each time point 2 x 200 µL of each culture were collected into a sterile 96-well plate (F-bottom) (Greiner Bio-One Cellstar) and then frozen at −80 °C ...
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bioRxiv - Immunology 2023Quote: ... 1,000,000 cells in 2 ml culturing media were used for the 25 mm diameter coverslips (in a 6-well cell culture plate Greiner Bio-One #657160) and 200,000 cells in 200 µl culturing media were added to the apical compartment of transwell (the basolateral compartment of transwells was filled with 600 µl culturing media) ...
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bioRxiv - Microbiology 2023Quote: ... was incubated with 50µL of magnetic beads coated with recombinant protein (2 µg protein/1.25 × 106 beads) in 96-well flat-bottom chimney plates (Greiner bio one, Frickenhausen, Germany) on a plate shaker at 300 rpm for 16 h at 4 °C in the dark ...
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bioRxiv - Immunology 2023Quote: ... Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One, 675061) as previously described (Hernández-Pérez et al. ...
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bioRxiv - Genetics 2023Quote: ... at 0.33 x 107 cells per well for the RNA sequencing experiment and 2 x 105 cells per well in a 96-well (M0562, Greiner, Sigma Aldrich, USA) for microscopy ...
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bioRxiv - Cancer Biology 2020Quote: HMEC-1 cells or HMEC-1 cells overexpressing xCT or SLC1A3 were seeded into 24-well plates (Greiner Bio-One) at a density of 6.5 × 103 cells per well in 0.5 mL medium ...
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bioRxiv - Cell Biology 2021Quote: ... 1% L-glutamine and 1% penicillin–streptomycin without phenol red (ThermoFischer) in glass-bottom CELLview culture dishes (Greiner Bio-One) for 24h and serum starved for 32h ...
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bioRxiv - Biophysics 2020Quote: ... 0.5 μM (gPRM)14 and 1 μM mMDM2 in KMEI buffer supplemented with 2% DMSO in a total volume of 20 μL in a 384-well microplate (Greiner Bio-One, Cat. 781090). High-throughput screening was performed using compounds in a commercial library (Selleck-2148) ...
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bioRxiv - Cancer Biology 2022Quote: ... After 96 hours, tumor organoids were shock-frozen in precooled 2-methylbutan (Carl Roth, Karlsruhe, Germany) using a magnetic pen (Greiner Bio-One, Kremsmünster, Austria) to transfer them into cryovials.
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bioRxiv - Biochemistry 2020Quote: ... progress was tracked by the change in absorbance at 340 nm by the formation or consumption of NADPH (ε340= 6,220 M-1 cm-1) in UV-transparent 96 well plates (Greiner, Sigma-Aldrich) measured using a SpectraMax Plus microplate reader (Molecular Devices).
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bioRxiv - Genomics 2021Quote: ... The cells were plated at a density of 2 × 106 cells per 75 cm2 tissue culture flasks (CELLSTAR® Greiner Bio-One GmbH, Frickenhausen, Germany) in Minimum Essential Medium Eagle culture medium (MEM ...
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bioRxiv - Cancer Biology 2021Quote: ... were seeded on the upper compartments of 2 mg ⁄ ml type I collagen-coated culture inserts (8 µm pores -Greiner Bio-One SAS, Courtaboeuf, France). DMEM supplemented with 10% FCS was used as a chemoattractant ...
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bioRxiv - Bioengineering 2021Quote: ... of CCB cells seeded in DMEM with a density of 60 000 cells cm-2 in a cell disc (1000 cm2, Greiner Bio-One GmbH, Kremsmünster, Germany). Infected cells were incubated at 25 °C for three days post infection (p.i. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The SARS-CoV-2 3CLpro assay was initiated by dispensing 2 µL/well of 50 nM enzyme solution into 1536 black bottom microplates (Greiner Bio-One, Monroe, NC, USA) by a Multidrop Combi disperser (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: 333 microliters of pre-reduced and filter-sterilized BHIS (with no added cysteine) were added to 2-mL 96-deep well plates (Greiner Bio-One, Cat. #1986-2110) using a semi-automated BenchSmart pipettor (Mettler-Toledo ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified protein with 25 mg/ml concentration was mixed with sparse matrix crystallization screening conditions in a 1:1 volumetric ratio by using 72 well Terasaki crystallization plates (Greiner-Bio, Germany). The mixture was sealed with 100% paraffin oil to slow down the vapor diffusion (Tekkim Kimya ...
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bioRxiv - Immunology 2021Quote: ... Cells were mixed (ratio 1:1) and plated at 8×103 per well in a μClear 96-well plate (Greiner Bio-One). The indicated SARS-CoV-2 strains were incubated with serially diluted mAb or sera for 15 minutes at room temperature and added to S-Fuse cells ...
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bioRxiv - Microbiology 2022Quote: ... Cells were mixed (ratio 1:1) and plated at 8×103 per well in a μClear 96-well plate (Greiner Bio-One). The indicated SARS-CoV-2 strains were incubated with mAb ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with Fibronectin (25 μg ml−1) was incubated at 1 hour at 37 °C to polymerize it in the inserts (8 μm ThinCert; Greiner bio-one). Inserts were then inverted and cells were seeded on the opposite side of the filter and allowed to attach to the matrix for 4h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... were mixed (ratio 1:1) and plated at a density of 8 × 103 per well in a μClear 96-well plate (Greiner Bio-One) as previously described (Buchrieser et al. ...
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bioRxiv - Immunology 2021Quote: ... Cells were mixed (ratio 1:1) and plated at 8×103 per well in a μClear 96-well plate (Greiner Bio-One). The indicated SARS-CoV-2 strains were incubated with monoclonal antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Cells were mixed (ratio 1:1) and plated at 8×103 per well in a μClear 96-well plate (Greiner Bio-One). The indicated SARS-CoV-2 strains were incubated with mAb ...
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bioRxiv - Microbiology 2022Quote: ... Cells were mixed (ratio 1:1) and plated at 8 × 103 per well in a μClear 96-well plate (Greiner Bio-One). The indicated SARS-CoV-2 strains were incubated with serially diluted monoclonal antibodies or sera for 15 min at room temperature and added to S-Fuse cells ...
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bioRxiv - Biochemistry 2023Quote: ... Product formation was monitored spectrophotometrically at 380 nm (ε = 15,800 M−1 cm−1) in individual 96-well plates (Greiner Bio-One) wells using a Biotek Synergy H1 multimode plate reader (Agilent) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were mixed (ratio 1:1) and plated at 8 × 103 per well in a μClear 96-well plate (Greiner Bio-One). The indicated SARS-CoV-2 strains were incubated with serially diluted monoclonal antibodies or sera for 15 min at room temperature and added to S-Fuse cells ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) in a 384-well microplate (Greiner) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Organoids were sheared into fragments prior to resuspension in ice-cold liquified BME-2 and seeded as droplets in pre-warmed non-treated CELLSTAR® 48-well suspension culture plates (Greiner Bio-One GmbH, Kremsmünster, AUT). Organoid-containing BME-2 droplets were polymerized for 15 min at 37°C after which the droplets were overlaid with hPO medium.
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bioRxiv - Microbiology 2022Quote: ... 20,000 cells were infected with HIV-1* or HIV-1*CA14SiR in a 96-well v-bottom microplate (Greiner Bio-one, cat. #650161) in a volume of 40 µl RPMI and transferred at 22 h p.i ...
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bioRxiv - Molecular Biology 2022Quote: Assays were performed as previously described where 80 nM UPF1-HD was pre-bound to 1 µM substrate29 but (Table S3) supplemented with 1 mM DTT and using clear 96 well half area plates (Greiner Bio-one 675101). All experiments were performed on a CLARIOstar Plus plate reader at 37°C with 1 mM ATP (BMG Labtech).