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Citations for Greiner :
251 - 290 of 290 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 100 μl 1x passive lysis buffer was added to the cell monolayer which was rocked for 15 min and then 10 μl of each lysate was transferred to tissue culture treated white microplates (Greiner Bio-One). Luciferase measurements were carried out in a FLUOstar Optima microplate reader (BMG Labtech Ltd) ...
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bioRxiv - Microbiology 2022Quote: ... Filtrate and diluted concentrate (1:100) were added to a black optical bottom 96 well plate (10 µL/well; Greiner Bio-One) in technical duplicate and each fraction was processed separately in the presence or absence of hydrolysis enzymes according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: Caco-2 cells cultured in DMEM supplemented with 10 % FBS were seeded at about 70 % confluency in nine 12-well plates (CELLSTAR, Greiner Bio-One) to test 3 KRAS mutant status (WT ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Ten μL of the test compound or 10% (v/v) DMSO /water were dispensed into a 96 well plate (Greiner Bio-One) and 50 μL of the enzyme (in assay buffer containing 1 % DMSO ...
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bioRxiv - Biochemistry 2022Quote: ... Binding reactions were incubated for 20 min on ice before 10 μL of the reactions were pipetted into a 384-well microplate (Greiner Bio-One). Fluorescence polarization was measured using a Tecan Spark multimode microplate reader using appropriate filter settings for Cy5 fluorescence.
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bioRxiv - Developmental Biology 2023Quote: ... One million cells/mL was seeded in a total of 5.5 mL mTesr Plus containing 10 μM Y-27632 in each well of non-tissue culture treated 6-well plates (Greiner Bio-One). The plates were placed on a shaker (25 mm orbit ...
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bioRxiv - Microbiology 2024Quote: ... Cells were mixed (ratio 1:1) (U2OS-ACE2 GFP1-10 (104) and GFP11 cells (104)) and plated in a μClear 96-well plate (Greiner Bio-One). Serial dilution of VHHs were incubated with the cells at 37°C and infected with a SARS-CoV-2 strain ...
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bioRxiv - Immunology 2024Quote: ... The cells were resuspended in phenol-free complete RPMI-10 media without antibiotic and plated in 96- well plates (Greiner BioOne: #655083) and 10,000 AM ...
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bioRxiv - Cell Biology 2024Quote: ... and HBEpC cells (500,000 cells) were plated in 10 cm (A549 and HCC44 cells) or 6 cm (HBEpC cells) cell culture dishes (Greiner Bio-One) in growth medium and incubated at 37 °C and 5 % CO2 overnight ...
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bioRxiv - Neuroscience 2024Quote: Cortical homogenates to a final volume of 10 μL were pipetted in triplicate into a 384-well (pure white, low volume, conical) proxiplate (Greiner Bio-One). Lysate dilutions and antibody concentrations are summarized in Supplementary Table 3 ...
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bioRxiv - Immunology 2024Quote: ... without heat-inactivation were serially diluted in D10 medium (DMEM containing 10% heat-inactivated FBS) and incubated with SIVmac239Δvif in white 96-well plates (Greiner, cat. 82050-736) at 6 ng of SIV p27 per well in a final volume of 100 μl per well ...
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bioRxiv - Microbiology 2024Quote: ... of 1x passive lysis buffer were added to the cell monolayers and rocked for 20 min at room temperature and then 10 μL of each lysate was transferred to tissue culture treated white microplates (Greiner Bio-One). Luciferase measurements were carried out using a FLUOstar Optima microplate reader (BMG Labtech Ltd) ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated with primary antibodies (1:100 in 5% BSA-PBS, Table S1) in 10 µL drops in a humidified Terasaki plate (Greiner Bio-One) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 10 µl of the cells were transferred to different wells of a 96-well plate (Greiner Bio-One™ CellStar™ μClear™) containing 140 µl of MSM with elevated ferulate concentrations (20 mM – 80 mM) ...
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bioRxiv - Immunology 2021Quote: ... 50 μl of serially diluted mouse sera were combined with 65 TCID50 PVs in 50 μl of complete medium (DMEM supplemented with 10% FBS and non-essential aminoacids) in 96 well plates (Greiner Bio-One, Germany) and incubated at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.2-7.4) and attached at the bottom of a plastic Petri dish lid (35×10 mm; Greiner Bio-One International GmbH, Austria) filled with HL3.1 ...
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bioRxiv - Biochemistry 2020Quote: ... 10000 Huh7 cells were seeded in 100 μl culture medium (DMEM with 10% FBS, 2mM L-glutamine, penicillin-streptomycin) in a 96-well white microplate (Greiner Bio-One, Austria) pre-coated with poly-D-lysine (10μg/ml ...
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bioRxiv - Synthetic Biology 2021Quote: ... samples were gently mixed (not to generate bubbles) using a multichannel pipette and 10 μL of each was transferred into a 384-well plate (Greiner Bio-One #784076). Note that the volume of mixtures should be at least 20% in excess in PCR tubes not to face difficulties in the final pipetting step into the 384-well plate ...
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bioRxiv - Molecular Biology 2023Quote: ... at a final concentration of 0.2% v/v and immediately transferred onto a 10-well cell culture microscopy slide (CellView; Greiner Group AG, Kremsmünster, Austria) pre-treated with poly-lysine (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... colonies were picked and grown overnight at 37 °C in 200 µL of NonSPI2 or 10% BHI medium containing 20 µg/mL Cm and 50 µg/mL Strep in flat-bottom 96-well plates (Greiner Bio-One #655161) sealed with breathable seals (Excel Scientific #LMT-AERAS-EX ...
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bioRxiv - Cancer Biology 2022Quote: Cells were plated at 300,000 cells/mL in RPMI medium with 10% serum at 2 mL per well in 6-well treated tissue culture plates (Greiner, Cat. # TCG-657160) to achieve 75% density the following day and incubated overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... T47D) in RPMI medium with 10% serum at 2 mL per well in 6-well treated tissue culture plates (Greiner, Cat. # TCG-657160) and incubated overnight ...
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bioRxiv - Microbiology 2022Quote: ... A 10 uL Neon™ pipette tip was used to extract 10 uL of the cell/gRNA/Cas9 solution which was then electroporated using the Neon™ Transfection System (voltage: 1650 V, width: 10 ms, pulses: 3) and transferred to a prepared 6-well dish (Greiner #82050-842) containing growth medium ...
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bioRxiv - Plant Biology 2024Quote: ... and incubated in 100 μL of 10 μM coelenterazine water solution in darkness overnight in white 96-well plates (Greiner Bio-One, #655075). After replacing the coelenterazine solution with 75 μL of distilled water ...
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bioRxiv - Bioengineering 2024Quote: ... of these pre-culture cells were inoculated into a 5 or 10-mL TB media supplemented with antibiotics in 50-mL falcon tube with a filter cap (Greiner Bio-One #227245) and cultured at 30°C or 25°C at 200 rpm for 48 hours using a TAITEC BR-43FL incubator shaker.
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bioRxiv - Cell Biology 2024Quote: ... 2nd generation virus was prepared in HEK293T cells grown to about 80% confluency in 10 cm cell culture dish (Greiner Bio-One; 664160). For each reaction ...
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bioRxiv - Systems Biology 2021Quote: ... which was later divided into three technical replicates of 10 μl during transferring to 384 well micro-plates (low binding microplate, 384 well, E18063G5, Greiner Bio-One, Kremsmünster, Austria). In total ...
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bioRxiv - Biochemistry 2021Quote: ... which was later divided into three technical replicates of 10 μl upon transferring to 384-well micro-plates (low binding microplate, 384 well, E18063G5, Greiner Bio-One, Kremsmünster, Austria). In total ...
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bioRxiv - Biophysics 2022Quote: ... solubilized wildtype rat receptors and C1/C2 heterodimers were further diluted 2- and 4-times in acquisition buffer together with increasing concentrations of Glutamate or Glutamate + 10 µM BINA on white 384 well plates (polystyrene, flat-bottom, small volume, medium-binding, Greiner Bio-One SAS, France). Dose-response curves at different time points after storage of the samples at RT were obtained by LRET acquisitions on a Spark 20M (Tecan ...
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bioRxiv - Neuroscience 2021Quote: ... 4.2 µl RNase-free water) was added to 10 µl RNA for each sample in a nuclease-free PCR tube (Greiner Bio-One, Monroe, NC). No reverse transcriptase and no RNA controls were also assessed by PCR ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: 200,000 mouse EpCAM+ primary cells and 200,000 CCL206 fibroblasts (n=4 independent isolations) were seeded in 1 ml Matrigel diluted 1:1 in DMEM containing 10% FBS in 6-well plates (Greiner Bio-One, The Netherlands). 12.000 or 30.000 nylon reference microfibers were mixed with Matrigel and cells prior to seeding ...
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bioRxiv - Cell Biology 2021Quote: ... The stroma was cut in 9 × 7 mm pieces for the dermis and 5 × 10 mm pieces for the submucosa and placed onto transparent ThinCertTM inserts of 0.4 μm pore size (Greiner Bio-One Ltd; Cat#657641). Immediately afterwards ...
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bioRxiv - Biochemistry 2024Quote: ... and the FUS solution was well-mixed and immediately dropped onto a glass bottom 10-compartment cell culture slide (CELLview, Cat. #543079, Greiner Bio-One, Kremsmünstern, Austria). FUS droplets were observed with the 473 nm laser line of a confocal microscope (FV1200 ...
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bioRxiv - Microbiology 2020Quote: ... Vero E6 cells were seeded at 3×104 in 100 μl DMEM+10% heat-inactivated FBS in 96-well Operetta plates (Greiner Bio-One, Monroe, NC, USA). The following day ...
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bioRxiv - Biophysics 2022Quote: ... A serial dilution of AcmJRL was dispensed in triplicates (10 μL each) in a black 384-well plate (Greiner Bio-One, Germany, cat no 781900). The solution of the fluorescent ligand (10 μL ...
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bioRxiv - Molecular Biology 2021Quote: ... Snails were exposed individually to the 10 to 12 miracidia in wells of a 12-well tissue culture plate (Greiner Bio-One, North Carolina, USA) at room temperature ...
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bioRxiv - Immunology 2023Quote: A volume of 10 mL of fresh blood was collected from all subjects on Acid Citrate Dextrose (ACD) tubes (Greiner Bio-One GmbH, Kremsmünster, Austria). For flow cytometry measurements ...
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bioRxiv - Microbiology 2024Quote: A serial dilution of EclA or EclA-C (10 µL, 0.4 – 400 µM) was transferred in triplicates to a black 384-well plate (Greiner Bio-One, Germany, catalog no. 781900) and then 10 µL of 20 nM of fluorescent ligand 11 in TBS/Ca buffer was added to each well ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were routinely propagated in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) in non-adherent flasks (Cellstar flasks for suspension culture, Greiner Bio One, Les Ulis, France). For routine culture ...
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bioRxiv - Biophysics 2022Quote: ... 10 μL of this mix was added to 10 μL previously prepared dilutions of the test compounds in black 384-well microtiter plates (Greiner Bio-One, Germany, cat. no. 781900) in triplicate ...