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Citations for Greiner :
201 - 250 of 254 citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The cells were then used to inoculate (1:20 dilution, starting Abs600nm = 0.025) a sterile 96-well microplate (Greiner Bio-one) containing 50% LB broth ...
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bioRxiv - Immunology 2019Quote: ... Blood was collected using plain hematocrit tubes (Drummond; 1-000-7500-C/50) into K2-EDTA tubes (Terumo CapiJect; T-MQK or Greiner; 450480) and processed the same day ...
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bioRxiv - Evolutionary Biology 2021Quote: ... tRNAscan-SE was also used to identify tRNA genes in the mitochondrial contigs of the S. chalcedonica assembly. The mitogenome plot (Supp. Fig. 1) was produced with OGDRAW version 1.3.1 (Greiner, et al. 2019). The annotated A ...
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bioRxiv - Immunology 2020Quote: ... For intracellular cytokine staining 1E6 peritoneal cells were incubated for 4.5 hours at 37°C in 200μl sterile RPMI containing Brefeldin A and Monensin (both 1:1000) in cell repellent 96 well plates (Greiner Bio-One) after which cells were washed once and stained on ice for extracellular and intracellular as described with one additional Fc blocking step (10 minutes on ice ...
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bioRxiv - Microbiology 2022Quote: Vero and IGROV-1 cells were plated at 30,000 cells per well in a mClear 96-well plate (Greiner Bio-One). The nasopharyngeal swabs were added to the Vero or IGROV-1 cells at serial dilutions from 1:10 to 1:31 250 as described previously 63 ...
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bioRxiv - Molecular Biology 2023Quote: ... Serially diluted PFI-7 and GID4-Tracer at a concentration of 1 μM were pipetted into white 384-well plates (Greiner 781207) using an Echo acoustic dispenser (Labcyte) ...
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bioRxiv - Microbiology 2023Quote: ... One milliliter of the solution containing the perturbation and 1 mL of the initial culture were combined in a 2-mL 96-deepwell plate (Greiner, 780270).
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bioRxiv - Biophysics 2022Quote: ... in PBS buffer was incubated with 25 μl of fresh stock of 3 mg/ml fluorescamine in DMSO at room temperature for 1 hour in a 96-well plate (Greiner 650209). Fluorescence intensity (λEx = 365 nm ...
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bioRxiv - Biophysics 2023Quote: ... Multicellular spheroids are formed from 4000 cells in 100 μl medium by culturing for 1 day in non-adhesive U-well plates (Greiner, Kremsmünster). The next day ...
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bioRxiv - Microbiology 2023Quote: ... One milliliter of the solution containing the perturbation and 1 mL of the initial culture were combined in a 2-mL 96-deepwell plate (Greiner, 780270).
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bioRxiv - Microbiology 2023Quote: Murine macrophages (C57BL/6 or J774A.1) were seeded at 2×105 cells/ml in 24 well plates (Greiner Bio-One). IFN-γ was added 24 hours prior to infection ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were washed twice with PBS and mixed together in 1 ml PBS in a 24-well plate (Greiner Bio-One GmbH) at a final OD600 of 0.7 for S ...
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bioRxiv - Microbiology 2023Quote: ... The cell-pellet was then resuspended in 1 ml of TR salts medium and 5 µl of this suspension inoculated into 195 µl TR medium with either carbon source (∼105 colony forming units (CFUs ml−1) in a 96-well plate (Greiner Bio). The optical density (600 nm ...
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bioRxiv - Cell Biology 2023Quote: ... 900 µl of cell suspension containing 1 × 10 ⁶ cells were kept in a 15 mL tube (Greiner Bio-One, Kremsmünster, Austria), which was positioned horizontally before 100 µl of CTV staining solution was added to the upper end of the tube without mixing ...
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bioRxiv - Microbiology 2020Quote: ... SupT1-R5 cells with AAV (expressing CPSF6 shRNA or non-silencing control shRNA) were distributed at 72 h post-transduction into 96-well plates (1 × 105 cells/well; U-bottom; Greiner Bio-One) and infected with wild-type HIV-1 (1 μUnits of RT/cell) ...
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bioRxiv - Biochemistry 2020Quote: ... 1-2 μl of beads per sample were pipetted into the wells of a 384-well glass-bottom microplate (Greiner Bio-One) pre-filled with a prep of ATTO390-SUVs in buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... A suspension of Ins-1 cells was pipetted in a 384-well culture black microplate with optic bottom plate (Greiner-Bio, 781090) at ~17,000 cells/well in 25uL of the low glucose DMEM culture medium ...
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bioRxiv - Genomics 2020Quote: Overnight cultures were diluted 100-fold in 1 mL of LB in a 96-deepwell plate (Masterblock 96 well, 2ml, V-bottom plates by Greiner Bio-one). After 2.5 h at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... Confrontations were carried out by spreading mixtures of both interaction partners at prey-predator ratios of 10:1 on 20 x wMY agar-plates (120 x 120 x 17 mm, Greiner Bio-One). At indicated time points cells were washed off the plate and centrifuged at 3000 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... Serially diluted inhibitor and Tracer molecule 19c at a concentration of 1 µM determined previously as the Tracer 19c KD,app were pipetted into white 384-well plates (Greiner 781 207) using an Echo acoustic dispenser (Labcyte) ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of RPMI-1640 was added to all the wells of columns 1-9 and 12 of a flat-bottom 96-well microtiter plate (Greiner Bio-One). 200 μL of RPMI-1640 + 12.8 mg/mL BA was added to all wells of column 11 and 200 μL of RPMI-1640 + 64 μg/mL FLC was added to all wells of column 10 ...
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bioRxiv - Immunology 2022Quote: ... NR-54970) were seeded at a density of 1×104 per well in half area 96-well black opaque plates (Greiner Bio-One) and were grown overnight at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Plant Biology 2021Quote: Seedlings were initially grown on 1/2 MS plates for 3 days before being transferred to 96-well plates (655075, Greiner Bio-One) in 100 μL liquid MS for 5 days ...
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bioRxiv - Plant Biology 2021Quote: Four-day-old seedlings growing on 1/2 MS plates were transferred into individual wells of a transparent 48-well tissue culture plate (Greiner Bio-One) containing 500 μL of liquid MS media with/without elicitor addition ...
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bioRxiv - Cell Biology 2019Quote: ... 50 mM Tris·HCl pH 7.4 and 1 mM DTT in a 20 µl volume in non-binding clear bottom 384 well plates (Greiner Bio-One, 781906). Compounds ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cultures were diluted 1:100 into 1 mL of LB/chloramphenicol medium with different concentration of bile salts in 96 deep-well plates (Greiner bio-one), incubated at 37°C with vigorous shaking for a further 4 hours and analyzed by flow cytometry ...
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bioRxiv - Microbiology 2022Quote: ... Filtrate and diluted concentrate (1:100) were added to a black optical bottom 96 well plate (10 µL/well; Greiner Bio-One) in technical duplicate and each fraction was processed separately in the presence or absence of hydrolysis enzymes according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Concentration of cells in the medium was estimated using a Neubauer chamber and a 100 μL of medium containing 1×106 cells /mL plated per well in a 96-well plate (Greiner Sensoplate, Germany) coated with Poly-D-Lysine (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5 µL of cells at 1 × 105 cells/mL (500 cells/well) were dispensed into a 1536-well white solid-bottom tissue treated plate (Greiner Bio-One) using a Multidrop Combi dispenser ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cells were then inoculated at the starting OD600= 1 in SDG medium using a six-well microplate (Greiner Bio-One™) and were incubated at 30 °C and 350 rpm in a thermomixer for 48 hours ...
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bioRxiv - Bioengineering 2023Quote: ... a 1:1 volume of reagent and culture medium was added to each sample of a black 96 well plate (655892, Greiner Bio-one), shaken for 10 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... or DMSO (columns 1-2, 23-26, and 47-48) was dispensed into 1536 well black polystyrene assay plates (Greiner, Kremsmünste, Austria) using an Echo 550 liquid dispenser (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: The stable cell line of SNAPf–TDP-43 was seeded in complete DMEM containing 10% FBS and 1× PSQ at a density of 5 ∼ 6 × 105 cells/dish in cell culture dishes (35 mm, Greiner Bio-One). Stock solutions of doxycycline (2 mg/mL in DMSO) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were counted by hemacytometer then seeded at a density of 1 million cells per well in 6-well poly-D-lysine pre-coated plates (Greiner Bio-One) and incubated at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 100 μl of cells was vigorously mixed with 100 μl of 1 mM D-luciferin in a white 96-well plate (655073, Greiner Bio-One), and light emission was immediately measured by the luminescence detection mode in Cytation 5 (Biotek) ...
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bioRxiv - Immunology 2024Quote: ... S-Fuse cells were mixed (at a 1:1 GFP1-10 / GFP11 ratio) and plated at 8 × 103 cells per well in a μClear 96-well plate (Greiner Bio-One). The tested SARS-CoV-2 strains were incubated with serially diluted chip effluent or supernatants from ECM digestion for 20 min at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and HSJD-GBM-001 (GBM1) were cultured in suspension in low-attachment 250 mL/75 cm2 flasks (Greiner #658, Supp. Table 1). The medium for DIPG4 ...
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bioRxiv - Biophysics 2020Quote: ... were collected by centrifugation at 114 RCF for 5 min and suspended in 1 ml of fresh medium in a cell culture-treated 12-well plate (#665180, Greiner Bio-One, Austria). The starting cell count was equal to 70,400 for target sample ...
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bioRxiv - Molecular Biology 2021Quote: ... and mixed with biotinylated RBD protein (final concentration 1 nM) in white low binding 384well microtitre plates (F-bottom, Greiner Cat nr 781904). As isotype control antibody ...
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bioRxiv - Pathology 2021Quote: ... diluted to a concentration of 1 mM and 40 nL were dispensed in 384 well plates (Greiner µClear #781091, Greiner Bio-One GmbH) using an acoustic liquid handler (Echo® 550 ...
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bioRxiv - Pathology 2021Quote: ... diluted to a concentration of 1 mM and 40 nL were dispensed in 384 well plates (Greiner µClear #781091, Greiner Bio-One GmbH) using an acoustic liquid handler (Echo® 550 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were prepared by aliquoting 180 μL of gel into ThinCert well inserts (1 μM pore size, Greiner Bio-One Ltd, Gloucestershire, UK). 900 μL of assay media was pipetted into the wells after 5 minutes and left to recover for 5 minutes before 100 μL of media was added to each insert ...
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bioRxiv - Biochemistry 2024Quote: ... After 1 µl of protein solution (10 mg/ml) was mixed with 1 µl of reservoir solution on a sitting plate (96-well CrystalQuick plates, Greiner Bio-One, Germany), the plate was incubated at 20 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were anesthetized in 0.015% tricaine- S and embedded dorsally in 1% LMP agarose in E3 medium + PTU in a lumox membrane-bottomed dish (Greiner Bio-one, Monroe, NC). Images were acquired on a Leica TCS SP8X laser-scanning confocal microscope equipped with LASX software ...
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bioRxiv - Cell Biology 2019Quote: ... 200 μL of this diluted culture was diluted 1:10 into YPD four times in a sterile 96-well plate (Greiner Bio-one, Kremsmünster, Austria). 5 μL of the serial dilutions were transferred from the 96-well plate onto YPD agar plates with or without additional 5 mM guanidinium hydrochloride (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... two-fold serial dilutions of recombinant Bma-LEC-1 and Bma-LEC-2 were prepared in V-bottom 96-well plates (Greiner Bio-One, Kremsmünster,Austria) followed by the addition of a 4% solution of trypsinized ...
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bioRxiv - Microbiology 2022Quote: ... albicans cells were prepared in 0.05×PDB at a concentration of 1×106 cells/mL and 100 μL was added to each well of a black polystyrene microtiter plate (Greiner Bio-One, Frickenhausen, Germany). Fluorescence intensity was followed for 7 min 30 sec at an excitation/emission wavelength of 622/670 nm using a multimode microplate reader (CLARIOstar Plus ...
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bioRxiv - Microbiology 2019Quote: ... were subcultured at a ratio of 1:2 every 2 - 4 weeks and maintained in 25 cm2 culture flasks (Cellstar; Greiner Bio-One, Monroe NC) at 32°C ...
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bioRxiv - Neuroscience 2021Quote: ... were seeded at a density of 1 × 106 cells/well onto tissue culture inserts containing porous membrane (1 µm pores; Greiner Bio-One GmbH, Kremsmünster, Austria) in a 6-well cell culture plates ...
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bioRxiv - Systems Biology 2022Quote: ... We picked three single colonies for each strain and inoculated them into separate wells containing 200 μL of LB (with 50 mg L-1 kanamycin for transposon insertion strains) in 96-well flat-bottom polypropylene plates (Greiner Bio-One ref: 655261) and incubated them at 30 °C overnight shaking at 800 rpm.