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Citations for Greiner :
101 - 148 of 148 citations for Alpha 2 HS glycoprotein Fetuin A Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... One milliliter of the solution containing the perturbation and 1 mL of the initial culture were combined in a 2-mL 96-deepwell plate (Greiner, 780270).
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bioRxiv - Microbiology 2023Quote: Forty microliters of thawed cryo-stocks were transferred from the 96-microwell plates of the progenitor collection to the condensed collection in 2-mL 96-deepwell plates (Greiner, 780270) previously inoculated with 1 mL of commercial MRS with 10 µg/mL tetracycline and pre-warmed at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... supernatants were removed from Caco-2-ACE2 and VeroE6-TMPRSS2 cells seeded previously in µClear 96 well microplates (Greiner Bio-One) and replaced with 100 µl of the virus:sera dilutions followed by incubation for 18 hours at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... One milliliter of the solution containing the perturbation and 1 mL of the initial culture were combined in a 2-mL 96-deepwell plate (Greiner, 780270).
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bioRxiv - Microbiology 2023Quote: Murine macrophages (C57BL/6 or J774A.1) were seeded at 2×105 cells/ml in 24 well plates (Greiner Bio-One). IFN-γ was added 24 hours prior to infection ...
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bioRxiv - Microbiology 2023Quote: ... with 2% (vol/vol) heat-inactivated FBS) and flushed through a 40 µm mesh cell strainer (Greiner Bio-One, Monroe, NC). GFP-expressing cells were sorted from non-transfected cells on a Becton Dickinson (BD ...
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bioRxiv - Microbiology 2024Quote: ... Plate was then incubated at 37°C + 5% CO2 for 2 hours before 60% of the wells’ supernatant was carefully transferred into a black-well plate (Greiner, #655076). A VarioskanTM LUX plate reader (Thermo Fisher Scientific #VL0000D0 ...
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bioRxiv - Biochemistry 2020Quote: ... 1-2 μl of beads per sample were pipetted into the wells of a 384-well glass-bottom microplate (Greiner Bio-One) pre-filled with a prep of ATTO390-SUVs in buffer containing 25 mM HEPES pH 7.5 ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... Samples were taken every hour for 8 hours and diluted to an OD600 of approximately 0.03 in phosphate-buffered saline (PBS) with 2 mg/mL of kanamycin sulfate in round bottom plates (Greiner Bio-One #650101). Samples were run on an Attune NxT Flow Cytometer via the autosampler on the same day and at least 20,000 events per sample were captured ...
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bioRxiv - Biophysics 2020Quote: ... cells were collected by centrifugation at 114 RCF for 5 min and transferred into 2 ml of fresh medium in a cell culture-treated 6-well plate (#657160, Greiner Bio-One) to provide for sufficient space for further growth.
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bioRxiv - Bioengineering 2021Quote: ... 2 μM of CDA5 or CDA5-Y34A was incubated in black flat bottom half volume opaque 96-well plates (Greiner Bio-One). For nucleotide response assays ...
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bioRxiv - Microbiology 2021Quote: ... ACE-2 transduced H1299-H2AZ cells were then sub-cloned at the single cell density in 384-well plates (Greiner Bio-One) in conditioned media derived from H1299 confluent cells ...
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bioRxiv - Microbiology 2020Quote: ... with the modification of mixing undiluted samples 1:1 with 2 mM TCEP in wells of a white 96-well plate (Greiner Bio-one) prior to use ...
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bioRxiv - Molecular Biology 2021Quote: ... spiked controls and diluted samples were mixed with 3 nM VHH72-h1 (S56A)-Flag3-His3 and 2.5 nM biotinylated SARS-CoV-2 RBD protein in white low binding 384-well microtitre plates (F-bottom, Greiner Cat nr 781904). After an incubation for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2021Quote: Seedlings were initially grown on 1/2 MS plates for 3 days before being transferred to 96-well plates (655075, Greiner Bio-One) in 100 μL liquid MS for 5 days ...
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bioRxiv - Plant Biology 2021Quote: Four-day-old seedlings growing on 1/2 MS plates were transferred into individual wells of a transparent 48-well tissue culture plate (Greiner Bio-One) containing 500 μL of liquid MS media with/without elicitor addition ...
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bioRxiv - Cell Biology 2021Quote: ... were produced by inoculation of VeroE6/TMPRSS2 cells and titred as previously described.(33, 36) Caco-2-ACE2 cells and cardiac PCs were plated in μClear 96-well microplates (Greiner Bio-one) and the next day infected with either REMRQ0001 or B.1.1.7 at multiplicity of infection (MOI ...
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bioRxiv - Immunology 2023Quote: HMVEC were seeded at 5 × 103 cells/well in EBM-2 cell culture medium in a flat bottom 96-well plate (Greiner Bio-One). After overnight settling of the cells ...
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bioRxiv - Genetics 2022Quote: ... Transfected cells were washed twice with 2 mL of extracellular solution (ECS, see below) and re-plated onto 35-mm polystyrene cell culture dishes (Cellstar, Greiner Bio-One). hKV1.3 currents were recorded from activated peripheral blood mononuclear cells (PBMCs ...
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bioRxiv - Cell Biology 2022Quote: hPOs were seeded in 5 µL BME-2 droplets in sterile CELLSTAR® 96-well suspension culture plates (Greiner Bio-One GmbH) and overlaid with 100 µL hPO medium ...
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bioRxiv - Biophysics 2023Quote: ... or DMSO (columns 1-2, 23-26, and 47-48) was dispensed into 1536 well black polystyrene assay plates (Greiner, Kremsmünste, Austria) using an Echo 550 liquid dispenser (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... Antigen-coupled microspheres were washed and incubated with plasma samples at an appropriate sample dilution (1:50 for Isotypes and 1:100 for all Fc-receptors) for 2 hours at 37°C in 384-well plates (Greiner Bio-One). Unbound antibodies were washed away ...
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bioRxiv - Cell Biology 2023Quote: ... epithelium was cut in pieces (up to 32 for a mouse esophagus) and placed on top of a transparent ThinCert™ insert (Greiner Bio-One, 657641) with the epithelium facing upward and the submucosa facing the membrane ...
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bioRxiv - Immunology 2021Quote: ... in 50 μl of DMEM containing 2% FBS were seeded in each well of black μCLEAR flat-bottom 96-well plate (Greiner Bio-one™). The cells were incubated overnight at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μL of two-fold serial dilutions of PdPV1 in PBS were incubated with an equal volume of 2% (v/v) erythrocytes in PBS in U-shaped microtiter plates (Greiner Bio One, Germany) at 37 °C for 2 h ...
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bioRxiv - Biochemistry 2021Quote: 50 µL of each purified soluble RBD variant at a protein concentration of 2 µg/ml was immobilized to Microlon clear plates (Greiner Bio-One 655081) at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: HEp-2 cells conjugated to NanoShuttlesTM were seeded on a 24-well ultralow-attachment plate (ULA, Cellstar® Greiner Bio-one, Kremsmünster, Austria) in 105 cells and a final volume of 400 μL/well.
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bioRxiv - Cancer Biology 2022Quote: Cells were plated at 300,000 cells/mL in RPMI medium with 10% serum at 2 mL per well in 6-well treated tissue culture plates (Greiner, Cat. # TCG-657160) to achieve 75% density the following day and incubated overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... T47D) in RPMI medium with 10% serum at 2 mL per well in 6-well treated tissue culture plates (Greiner, Cat. # TCG-657160) and incubated overnight ...
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bioRxiv - Microbiology 2023Quote: ... at each time point 2 x 200 µL of each culture were collected into a sterile 96-well plate (F-bottom) (Greiner Bio-One Cellstar) and then frozen at −80 °C ...
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bioRxiv - Immunology 2023Quote: ... 1,000,000 cells in 2 ml culturing media were used for the 25 mm diameter coverslips (in a 6-well cell culture plate Greiner Bio-One #657160) and 200,000 cells in 200 µl culturing media were added to the apical compartment of transwell (the basolateral compartment of transwells was filled with 600 µl culturing media) ...
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bioRxiv - Microbiology 2023Quote: ... was incubated with 50µL of magnetic beads coated with recombinant protein (2 µg protein/1.25 × 106 beads) in 96-well flat-bottom chimney plates (Greiner bio one, Frickenhausen, Germany) on a plate shaker at 300 rpm for 16 h at 4 °C in the dark ...
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bioRxiv - Immunology 2023Quote: ... Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One, 675061) as previously described (Hernández-Pérez et al. ...
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bioRxiv - Genetics 2023Quote: ... at 0.33 x 107 cells per well for the RNA sequencing experiment and 2 x 105 cells per well in a 96-well (M0562, Greiner, Sigma Aldrich, USA) for microscopy ...
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bioRxiv - Microbiology 2023Quote: ... were diluted in DMEM and thirty microliters of each tested concentration were manually dispensed into a well (with 2-3 replicates for each concentration) of a 96-well white plate (Greiner Bio-One, NC). Thirty microliters of viruses pseudotyped with specific Envs were then added ...
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bioRxiv - Microbiology 2023Quote: ... 1- or 2-μl droplets were deposited in the wells of a 96-well plate with a non-binding surface (Greiner Bio-One, 655901), one droplet per well ...
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bioRxiv - Microbiology 2023Quote: ... was diluted to 100-fold the working concentration in DMSO (2 mM) in V-bottom polypropylene plates (Greiner Bio-One, cat. No. 651261). For the screening experiments ...
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bioRxiv - Biophysics 2020Quote: ... 0.5 μM (gPRM)14 and 1 μM mMDM2 in KMEI buffer supplemented with 2% DMSO in a total volume of 20 μL in a 384-well microplate (Greiner Bio-One, Cat. 781090). High-throughput screening was performed using compounds in a commercial library (Selleck-2148) ...
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bioRxiv - Immunology 2022Quote: ... two-fold serial dilutions of recombinant Bma-LEC-1 and Bma-LEC-2 were prepared in V-bottom 96-well plates (Greiner Bio-One, Kremsmünster,Austria) followed by the addition of a 4% solution of trypsinized ...
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bioRxiv - Cancer Biology 2022Quote: ... After 96 hours, tumor organoids were shock-frozen in precooled 2-methylbutan (Carl Roth, Karlsruhe, Germany) using a magnetic pen (Greiner Bio-One, Kremsmünster, Austria) to transfer them into cryovials.
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bioRxiv - Cell Biology 2023Quote: ... vivax cases were infected into PHH lot BGW at day 2 post-seed (for case 1) or day 3 post-seed (for cases 2 and 3) in 384-well plates (Greiner Bio-One cat 781956) using the same methods for initiating P ...
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bioRxiv - Genomics 2021Quote: ... The cells were plated at a density of 2 × 106 cells per 75 cm2 tissue culture flasks (CELLSTAR® Greiner Bio-One GmbH, Frickenhausen, Germany) in Minimum Essential Medium Eagle culture medium (MEM ...
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bioRxiv - Cancer Biology 2021Quote: ... were seeded on the upper compartments of 2 mg ⁄ ml type I collagen-coated culture inserts (8 µm pores -Greiner Bio-One SAS, Courtaboeuf, France). DMEM supplemented with 10% FCS was used as a chemoattractant ...
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bioRxiv - Bioengineering 2021Quote: ... of CCB cells seeded in DMEM with a density of 60 000 cells cm-2 in a cell disc (1000 cm2, Greiner Bio-One GmbH, Kremsmünster, Germany). Infected cells were incubated at 25 °C for three days post infection (p.i. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The SARS-CoV-2 3CLpro assay was initiated by dispensing 2 µL/well of 50 nM enzyme solution into 1536 black bottom microplates (Greiner Bio-One, Monroe, NC, USA) by a Multidrop Combi disperser (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: 333 microliters of pre-reduced and filter-sterilized BHIS (with no added cysteine) were added to 2-mL 96-deep well plates (Greiner Bio-One, Cat. #1986-2110) using a semi-automated BenchSmart pipettor (Mettler-Toledo ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A new 18G butterfly catheter attached to a new 3 mL syringe was then used to draw the experimental 1-2 mL blood sample and was immediately transferred to EDTA tubes (Greiner Bio-One, Monroe, NC, USA) at times 0.25 ...
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bioRxiv - Cell Biology 2022Quote: ... Organoids were sheared into fragments prior to resuspension in ice-cold liquified BME-2 and seeded as droplets in pre-warmed non-treated CELLSTAR® 48-well suspension culture plates (Greiner Bio-One GmbH, Kremsmünster, AUT). Organoid-containing BME-2 droplets were polymerized for 15 min at 37°C after which the droplets were overlaid with hPO medium.