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Citations for Greiner :
51 - 100 of 2620 citations for E 7 Bromo 3 4 dihydrobenzo b oxepin 5 2H one O 2 2 2 trifluoroethyl oxime since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... supernatants were removed from Caco-2-ACE2 and VeroE6-TMPRSS2 cells seeded previously in µClear 96 well microplates (Greiner Bio-One) and replaced with 100 µl of the virus:sera dilutions followed by incubation for 18 hours at 37°C in 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... A 96-well 2-mL culture plate (Greiner) was filled with a concentrate of theophylline and L-rhamnose and was diluted with LB medium containing kanamycin and overnight grown bacteria to reach a final kanamycin concentration of 50 mg/L ...
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bioRxiv - Biochemistry 2020Quote: ... 1-2 μl of beads per sample were pipetted into the wells of a 384-well glass-bottom microplate (Greiner Bio-One) pre-filled with a prep of ATTO390-SUVs in buffer containing 25 mM HEPES pH 7.5 ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... Samples were taken every hour for 8 hours and diluted to an OD600 of approximately 0.03 in phosphate-buffered saline (PBS) with 2 mg/mL of kanamycin sulfate in round bottom plates (Greiner Bio-One #650101). Samples were run on an Attune NxT Flow Cytometer via the autosampler on the same day and at least 20,000 events per sample were captured ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μM of CDA5 or CDA5-Y34A was incubated in black flat bottom half volume opaque 96-well plates (Greiner Bio-One). For nucleotide response assays ...
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bioRxiv - Microbiology 2021Quote: ... ACE-2 transduced H1299-H2AZ cells were then sub-cloned at the single cell density in 384-well plates (Greiner Bio-One) in conditioned media derived from H1299 confluent cells ...
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bioRxiv - Microbiology 2020Quote: ... with the modification of mixing undiluted samples 1:1 with 2 mM TCEP in wells of a white 96-well plate (Greiner Bio-one) prior to use ...
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bioRxiv - Plant Biology 2021Quote: Four-day-old seedlings growing on 1/2 MS plates were transferred into individual wells of a transparent 48-well tissue culture plate (Greiner Bio-One) containing 500 μL of liquid MS media with/without elicitor addition ...
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bioRxiv - Cell Biology 2021Quote: ... were produced by inoculation of VeroE6/TMPRSS2 cells and titred as previously described.(33, 36) Caco-2-ACE2 cells and cardiac PCs were plated in μClear 96-well microplates (Greiner Bio-one) and the next day infected with either REMRQ0001 or B.1.1.7 at multiplicity of infection (MOI ...
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bioRxiv - Genetics 2022Quote: ... Transfected cells were washed twice with 2 mL of extracellular solution (ECS, see below) and re-plated onto 35-mm polystyrene cell culture dishes (Cellstar, Greiner Bio-One). hKV1.3 currents were recorded from activated peripheral blood mononuclear cells (PBMCs ...
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bioRxiv - Immunology 2023Quote: ... Antigen-coupled microspheres were washed and incubated with plasma samples at an appropriate sample dilution (1:50 for Isotypes and 1:100 for all Fc-receptors) for 2 hours at 37°C in 384-well plates (Greiner Bio-One). Unbound antibodies were washed away ...
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bioRxiv - Immunology 2024Quote: ... Antigen-coupled microspheres were washed and incubated with plasma samples at an appropriate sample dilution (1:1000 for all Isotypes and Fc-receptors) for 2 hours at 37°C in 384-well plates (Greiner Bio-One). Unbound antibodies were washed away ...
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bioRxiv - Immunology 2024Quote: ... 2-fold serial dilutions were prepared in PBS-T plus 1% BSA in non-binding 96-well plates (Greiner Bio-One, #655901) in triplicate at a starting dilution of 1:50 and a final volume of 60 μL/well ...
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bioRxiv - Immunology 2021Quote: ... in 50 μl of DMEM containing 2% FBS were seeded in each well of black μCLEAR flat-bottom 96-well plate (Greiner Bio-one™). The cells were incubated overnight at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μL of two-fold serial dilutions of PdPV1 in PBS were incubated with an equal volume of 2% (v/v) erythrocytes in PBS in U-shaped microtiter plates (Greiner Bio One, Germany) at 37 °C for 2 h ...
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bioRxiv - Biochemistry 2021Quote: 50 µL of each purified soluble RBD variant at a protein concentration of 2 µg/ml was immobilized to Microlon clear plates (Greiner Bio-One 655081) at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: HEp-2 cells conjugated to NanoShuttlesTM were seeded on a 24-well ultralow-attachment plate (ULA, Cellstar® Greiner Bio-one, Kremsmünster, Austria) in 105 cells and a final volume of 400 μL/well.
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bioRxiv - Microbiology 2023Quote: ... at each time point 2 x 200 µL of each culture were collected into a sterile 96-well plate (F-bottom) (Greiner Bio-One Cellstar) and then frozen at −80 °C ...
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bioRxiv - Immunology 2023Quote: ... 1,000,000 cells in 2 ml culturing media were used for the 25 mm diameter coverslips (in a 6-well cell culture plate Greiner Bio-One #657160) and 200,000 cells in 200 µl culturing media were added to the apical compartment of transwell (the basolateral compartment of transwells was filled with 600 µl culturing media) ...
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bioRxiv - Microbiology 2023Quote: ... was incubated with 50µL of magnetic beads coated with recombinant protein (2 µg protein/1.25 × 106 beads) in 96-well flat-bottom chimney plates (Greiner bio one, Frickenhausen, Germany) on a plate shaker at 300 rpm for 16 h at 4 °C in the dark ...
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bioRxiv - Microbiology 2023Quote: ... was diluted to 100-fold the working concentration in DMSO (2 mM) in V-bottom polypropylene plates (Greiner Bio-One, cat. No. 651261). For the screening experiments ...
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bioRxiv - Immunology 2023Quote: ... Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One, 675061) as previously described (Hernández-Pérez et al. ...
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bioRxiv - Genomics 2020Quote: ... Samples were processed in 2 mL microcentrifuge tubes (Greiner) containing two steel ball bearings (5/32” type 2B ...
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bioRxiv - Synthetic Biology 2020Quote: ... 2 mL in 14-mL round-bottom tubes (Greiner) (Figure 6A-C ...
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bioRxiv - Cell Biology 2022Quote: ... 4 compartments (Greiner Bio-One, 627870), two days before imaging ...
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bioRxiv - Biophysics 2020Quote: ... 0.5 μM (gPRM)14 and 1 μM mMDM2 in KMEI buffer supplemented with 2% DMSO in a total volume of 20 μL in a 384-well microplate (Greiner Bio-One, Cat. 781090). High-throughput screening was performed using compounds in a commercial library (Selleck-2148) ...
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bioRxiv - Immunology 2022Quote: ... two-fold serial dilutions of recombinant Bma-LEC-1 and Bma-LEC-2 were prepared in V-bottom 96-well plates (Greiner Bio-One, Kremsmünster,Austria) followed by the addition of a 4% solution of trypsinized ...
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bioRxiv - Cancer Biology 2022Quote: ... After 96 hours, tumor organoids were shock-frozen in precooled 2-methylbutan (Carl Roth, Karlsruhe, Germany) using a magnetic pen (Greiner Bio-One, Kremsmünster, Austria) to transfer them into cryovials.
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bioRxiv - Physiology 2021Quote: ... and plasma into 2 mL EDTA tubes (Vacuette®, Greiner Bio-One Brasil Produtos Médicos Hospitalares Ltda ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2% DMSO (v/v) in a 384-well microplate (Greiner). A final concentration of 50 µM Boc-Lys(TFA)-AMC was added and incubated for 1 hour at room temperature followed by the addition of developer solution containing a final concentration of 50 µM TSA ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were cultured in 2 L roller bottles (Greiner) at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... 2×107 cells were seeded into T75 culture flasks (Greiner) and cultured in the presence of 100 ng/mL recombinant mouse M-CSF (Peprotech ...
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bioRxiv - Biochemistry 2024Quote: ... 2 g/L BSA) were mixed in a microplate (Greiner, 384-well ...
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bioRxiv - Biochemistry 2024Quote: ... 2 g/L BSA) was prepared in a microplate (Greiner, 384 well ...
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bioRxiv - Genomics 2021Quote: ... The cells were plated at a density of 2 × 106 cells per 75 cm2 tissue culture flasks (CELLSTAR® Greiner Bio-One GmbH, Frickenhausen, Germany) in Minimum Essential Medium Eagle culture medium (MEM ...
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bioRxiv - Cancer Biology 2021Quote: ... were seeded on the upper compartments of 2 mg ⁄ ml type I collagen-coated culture inserts (8 µm pores -Greiner Bio-One SAS, Courtaboeuf, France). DMEM supplemented with 10% FCS was used as a chemoattractant ...
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bioRxiv - Bioengineering 2021Quote: ... of CCB cells seeded in DMEM with a density of 60 000 cells cm-2 in a cell disc (1000 cm2, Greiner Bio-One GmbH, Kremsmünster, Germany). Infected cells were incubated at 25 °C for three days post infection (p.i. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The SARS-CoV-2 3CLpro assay was initiated by dispensing 2 µL/well of 50 nM enzyme solution into 1536 black bottom microplates (Greiner Bio-One, Monroe, NC, USA) by a Multidrop Combi disperser (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: 333 microliters of pre-reduced and filter-sterilized BHIS (with no added cysteine) were added to 2-mL 96-deep well plates (Greiner Bio-One, Cat. #1986-2110) using a semi-automated BenchSmart pipettor (Mettler-Toledo ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A new 18G butterfly catheter attached to a new 3 mL syringe was then used to draw the experimental 1-2 mL blood sample and was immediately transferred to EDTA tubes (Greiner Bio-One, Monroe, NC, USA) at times 0.25 ...
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bioRxiv - Microbiology 2024Quote: ... Plate was then incubated at 37°C + 5% CO2 for 2 hours before 60% of the wells’ supernatant was carefully transferred into a black-well plate (Greiner, #655076). A VarioskanTM LUX plate reader (Thermo Fisher Scientific #VL0000D0 ...
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bioRxiv - Cell Biology 2022Quote: ... Organoids were sheared into fragments prior to resuspension in ice-cold liquified BME-2 and seeded as droplets in pre-warmed non-treated CELLSTAR® 48-well suspension culture plates (Greiner Bio-One GmbH, Kremsmünster, AUT). Organoid-containing BME-2 droplets were polymerized for 15 min at 37°C after which the droplets were overlaid with hPO medium.
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bioRxiv - Cancer Biology 2024Quote: ... and ∼2 x 104 cells (in 100 µL) were seeded on the upper compartment of each trans-well insert (Greiner Bio-One ThinCert, 8 µm pore) that had been treated for 30-60 min with a coating solution (1X PBS ...
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bioRxiv - Immunology 2021Quote: ... 1 ml was transferred into a 2 ml cryovial (Greiner, Belgium). The vials were frozen using a Mr ...
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bioRxiv - Microbiology 2020Quote: ... 5 mL (Greiner Bio-One, catalog # 606 180).
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bioRxiv - Molecular Biology 2023Quote: ... U-2-OS Flp-In cells grown in 96-well plates (Greiner) were treated with 1μg/ml tetracycline for 24 hours to induce expression of GFP-tagged SPT2 ...
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bioRxiv - Plant Biology 2021Quote: ... Four-mm leaf discs from 4th or 5th leaves of 5-week-old Nb plants were washed in double-distilled (mQ) water for 2h and incubated in 200 μl of mQ water in 96-well plates (Greiner Bio-One, #655075) under aluminum foil overnight ...
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bioRxiv - Cell Biology 2023Quote: NHDFs (passage 4-10) were seeded at 3 x 104/well on gelatinised glass-bottomed 24-well plates (662892; Greiner Bio-One) in FGM and cultured for 3 days ...
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bioRxiv - Molecular Biology 2020Quote: ... prepared as 2 μg/ mL solutions in black-bottom 96-well plates (Greiner), with 100 μL total volume per well ...
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bioRxiv - Neuroscience 2023Quote: ... in a 4-well petri dish (Greiner Bio One, #627170) and were cultured in DMEM/F12 – Glutamax (GIBCO ...